Phospholipase c eta 1 (PLCH1) (NM_001130960) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	PLC-L3; PLC eta 1; PLCL3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC326398 representing NM_001130960. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCAGACCTTGAAGTGTATAAAAACTTAAGTCCAGAAAAAGTTGAAAGATGCATGAGTGTGATGCAG TCCGGGACACAGATGATCAAGCTGAAACGTGGAACCAAAGGGCTTGTCCGCCTCTTTTACCTGGATGAG CACCGGACACGCCTCCGATGGCGACCCTCTAGGAAGAGTGAGAAGGCAAAAATACTTATTGATTCCATT TACAAAGTGACTGAGGGCCGGCAGTCTGAAATATTCCACAGACAAGCTGAGGGGAACTTCGACCCCAGC TGCTGCTTCACCATCTACCATGGCAACCACATGGAGTCCCTGGACCTCATCACCTCCAACCCCGAGGAG GCCCGCACCTGGATCACAGGCCTCAAGTACCTGATGGCTGGCATCAGTGATGAAGACTCCCTTGCCAAA AGGCAGAGGACCCATGACCAATGGGTGAAGCAGACCTTTGAGGAAGCTGATAAGAATGGTGACGGCTTG CTGAATATTGAAGAGATACATCAGCTGATGCATAAACTGAATGTTAATCTGCCCCGAAGAAAAGTCAGA CAAATGTTTCAGGAAGCCGACACAGATGAGAATCAGGGAACTTTGACATTTGAAGAGTTCTGTGTTTTT TACAAAATGATGTCTTTGAGACGAGACCTTTATTTGTTACTTTTGAGCTACAGTGACAAGAAAGATCAC CTAACTGTGGAAGAACTGGCTCAGTTTTTGAAGGTGGAGCAAAAGATGAATAATGTGACAACGGACTAT TGTCTTGACATCATAAAGAAGTTTGAAGTTTCAGAAGAAAATAAGGTGAAAAATGTTCTTGGCATAGAA GGCTTCACGAACTTCATGCGTAGTCCTGCCTGTGACATATTTAACCCATTGCACCATGAAGTGTACCAA GACATGGATCAGCCCCTCTGCAACTACTACATTGCTTCCTCTCACAATACATACCTGACTGGAGACCAG CTCCTTTCTCAGTCCAAAGTGGATATGTATGCACGGGTGCTGCAAGAGGGCTGTCGCTGTGTGGAAGTT GACTGTTGGGATGGCCCAGATGGAGAGCCAGTAGTACATCATGGTTACACTCTCACTTCAAAAATTCTC TTCAGAGATGTTGTGGAGACCATCAACAAGCATGCCTTTGTGAAGAATGAGTTTCCTGTTATATTGTCT ATCGAGAATCACTGCAGTATCCAGCAGCAAAGGAAGATTGCTCAGTACCTGAAAGGAATATTCGGAGAC AAACTGGACCTGTCATCTGTTGATACAGGGGAGTGCAAGCAGCTTCCAAGCCCTCAAAGTTTGAAAGGC AAAATTCTAGTGAAGGGTAAGAAGTTGCCTTATCACCTTGGGGATGATGCAGAGGAAGGGGAAGTTTCT GATGAGGACAGTGCAGATGAAATTGAAGACGAGTGCAAATTCAAGCTCCATTATAGTAATGGGACCACT GAGCATCAGGTGGAATCTTTCATAAGGAAAAAACTGGAGTCACTGTTAAAAGAATCTCAAATTCGAGAT AAAGAAGATCCTGATAGTTTCACAGTGCGGGCACTACTGAAGGCCACGCATGAAGGCTTAAATGCACAC CTGAAGCAGAGTCCAGATGTAAAGGAAAGTGGAAAGAAATCACATGGACGATCCCTCATGACCAACTTT GGAAAACATAAGAAAACTACAAAATCACGGTCTAAATCTTACAGTACTGATGATGAGGAAGACACACAG CAGAGTACTGGCAAGGAGGGTGGCCAGCTGTACAGATTGGGTCGCCGAAGGAAAACCATGAAGCTCTGC CGAGAACTCTCTGATTTGGTTGTGTACACAAACTCCGTGGCCGCTCAGGACATTGTGGATGACGGAACC ACAGGAAATGTGTTATCATTCAGTGAAACAAGAGCACATCAGGTTGTTCAGCAAAAATCAGAGCAGTTC ATGATTTATAATCAAAAGCAACTCACGAGGATTTACCCCTCTGCCTACCGCATTGATTCCAGTAACTTC AACCCTCTCCCCTACTGGAACGCAGGCTGCCAGCTAGTGGCACTGAATTATCAATCTGAAGGACGAATG ATGCAGTTAAACCGAGCCAAATTCAAGGCAAATGGCAATTGTGGCTATGTCCTCAAACCCCAGCAAATG TGCAAAGGTACTTTCAACCCTTTCTCTGGTGACCCTCTTCCTGCCAACCCCAAAAAGCAGCTCATCCTG AAAGTTATCAGTGGACAGCAACTCCCCAAACCTCCAGACTCCATGTTTGGAGATCGAGGCGAGATCATT GACCCTTTTGTTGAAGTTGAAATTATTGGATTGCCAGTAGATTGTTGTAAAGATCAAACCCGTGTGGTA GATGACAATGGATTTAACCCTGTGTGGGAAGAAACACTGACATTTACAGTACACATGCCAGAAATAGCT TTGGTTCGGTTCCTTGTGTGGGATCACGATCCCATTGGACGAGACTTTGTTGGACAAAGAACTGTGACC TTCAGCAGCTTAGTGCCTGGCTACCGGCATGTCTATTTGGAAGGACTGACAGAAGCATCCATATTTGTA CACATAACCATCAATGAAATCTATGGAAAGTGGAGCCCTTTAATACTCAACCCCAGTTATACTATATTG CACTTTCTAGGAGCTACAAAGAACAGACAACTCCAGGGTCTGAAGGGACTGTTCAATAAGAATCCTAGG CACAGTTCTTCAGAAAACAATTCCCATTATGTACGGAAGCGATCCATTGGAGATAGAATTCTGCGACGC ACAGCTAGCGCCCCAGCCAAAGGCAGGAAAAAGAGCAAAATGGGCTTCCAAGAAATGGTGGAGATAAAG GATTCTGTGTCCGAGGCCACAAGAGATCAAGATGGCGTGCTGAGGAGGACCACACGCAGTTTGCAAGCA CGCCCTGTCTCTATGCCTGTTGACAGAAACCTTCTGGGAGCTTTGTCGCTGCCTGTATCTGAAACAGCA AAAGACATTGAAGGAAAAGAAAACTCTCTAGCAGAAGATAAAGATGGCAGAAGAAAAGGGAAAGCAAGT ATAAAAGATCCACATTTTCTAAATTTCAACAAAAAGTTATCATCCTCCTCCAGTGCTCTGCTCCACAAA GATACCAGCCAAGGGGACACCATTGTATCTACTGCCCACATGTCAGTCACAGGAGAACAGCTGGGCATG TCAAGTCCTAGGGGTGGGAGAACCACATCAAATGCCACAAGCAATTGCCAGGAAAACCCCTGTCCCAGC AAGTCTCTCTCCCCAAAGCAGCATTTGGCTCCCGATCCTGTAGTTAACCCCACACAAGATCTGCATGGT GTGAAAATCAAGGAAAAGGGTAATCCTGAGGACTTTGTGGAAGGGAAAAGCATCTTGTCAGGAAGCGTC CTTTCTCATAGCAACCTAGAAATTAAGAACCTGGAAGGTAATAGGGGTAAGGGCCGAGCTGCAACATCC TTTTCTTTGTCAGACGTCTCCATGCTCTGTTCTGACATACCTGACCTACATTCAACTGCAATTCTGCAG GAGAGTGTAATTTCCCATCTTATTGACAATGTCACTTTAACAAATGAGAATGAGCCGGGCAGTTCCATC TCAGCCCTGATTGGCCAGTTTGATGAGACCAACAATCAGGCTCTCACAGTTGTTTCTCATCTTCATAAT ACCAGTGTGATGTCAGGCCATTGTCCCTTGCCTAGCCTGGGCCTAAAAATGCCCATCAAGCATGGTTTT TGCAAGGGAAAATCCAAGTCTTCCTTCCTGTGCTCATCTCCGGAGCTGATAGCACTCTCGAGTTCTGAG ACCACCAAACATGCAACGAACACAGTTTATGAAACTACCTGCACTCCCATCTCTAAAACCAAACCAGAT GATGACCTTTCTAGTAAGGCCAAGACAGCGGCCTTAGAAAGCAACCTGCCTGGATCCCCTAATACTTCT CGTGGCTGGTTACCAAAAAGTCCTACCAAGGGAGAAGACTGGGAAACACTGAAGAGCTGCAGCCCTGCC TCTTCCCCTGATTTGACCCTGGAGGATGTAATAGCTGATCCCACTCTCTGTTTCAATTCTGGGGAGAGC AGCCTTGTGGAAATTGATGGAGAATCAGAAAATCTTTCTCTAACAACCTGTGAATATAGGAGAGAGGGC ACAAGTCAACTTGCTTCTCCTTTAAAACTCAAGTACAATCAGGGTGTGGTAGAACACTTTCAAAGAGGT TTGAGAAACGGCTACTGTAAAGAGACCCTCCGCCCTTCTGTCCCTGAAATATTCAACAATATTCAAGAT GTCAAAACTCAAAGTATTTCTTATCTAGCCTATCAGGGTGCTGGCTTTGTGCATAATCATTTCTCAGAT TCAGATGCAAAAATGTTCCAGACCTGTGTGCCCCAGCAATCTAGTGCTCAAGATATGCATGTCCCTGTA CCCAAGCAGTTGGCACATCTTCCTTTGCCTGCTCTGAAACTGCCTAGTCCTTGCAAATCCAAAAGTCTG GGGGACTTAACATCAGAGGACATTGCCTGCAATTTTGAGAGCAAGTACCAGTGTATTAGTAAGAGTTTT GTTACAACTGGCATTAGAGACAAGAAGGGCGTGACTGTGAAGACAAAGTCGTTAGAGCCTATAGATGCC CTGACCGAGCAGCTTCGGAAGCTTGTGTCCTTTGACCAGGAAGACAACTGCCAAGTGCTATATTCAAAG CAGGATGCCAATCAGCTCCCCCGGGCACTGGTCAGGAAGTTGTCATCCAGAAGTCAGAGCAGAGTGCGC AATATTGCTAGTCGTGCCAAGGAGAAACAGGAAGCCAACAAGCAGAAAGTTCCAAACCCCAGCAATGGG GCAGGAGTGGTTCTTAGAAACAAACCCTCAGCACCCACCCCTGCAGTGAATCGCCACTCCACCGGCTCC TACATCGCAGGCTACCTGAAGAACACGAAAGGGGGTGGCCTTGAAGGCCGGGGCATCCCAGAGGGGGCA TGCACGGCTCTTCACTATGGCCACGTTGACCAGTTTTGTTCAGATAATTCTGTTTTGCAGACTGAGCCA AGCAGTGATGATAAACCAGAAATTTATTTTCTTTTGAGACTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001130960
Insert Size	5082 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001130960.1
RefSeq Size	6445 bp
RefSeq ORF	5082 bp
Locus ID	23007
UniProt ID	Q4KWH8
Protein Families	Druggable Genome
MW	189.2 kDa
Gene Summary	PLCH1 is a member of the PLC-eta family of the phosphoinositide-specific phospholipase C (PLC) superfamily of enzymes that cleave phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) to generate second messengers inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG) (Hwang et al., 2005 [PubMed 15702972]).[supplied by OMIM, Jun 2009] Transcript Variant: This variant (1) encodes the longest isoform (a).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.