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p114RhoGEF (ARHGEF18) (NM_001130955) Human Untagged Clone
CAT＃: SC326348
ARHGEF18 (untagged)-Human Rho/Rac guanine nucleotide exchange factor (GEF) 18 (ARHGEF18), transcript variant 2
CNY 22,520.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | P114-RhoGEF; p114RhoGEF; RP78; SA-RhoGEF |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC326348 representing NM_001130955.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGTCACAGTGGGGACCAATATCCTGCCCTCCAGACCCGCTGCTTCAGCCAATACAGCAAGGGAAGAC GCAGCTCTGTTTTCTAGAAGGATCCCACCGAGGCATAAAAACGGCGCAGCCCAGCCTGGCGCCGCGCCG GGTCCCGGAGCCCCGGGCGCGAACATGGGGAATGCGCACTCCAAAAGCGGGGACAGGCACAGCGCGCTC CCCGGCCGCCCCGAGCTGTCTTTTTACGGCTCTTTCCCCAGGAAATGGAGCGAGAACGTCTTCTTGGAT AACGAGCTGCTGACCTCCAAGATCCTGTCTGTGCTGCGGCCGCAGTCGGAGCGGGGCTTCCGCGCCGGG GACCTCCGCTACCCGACCCACTTTCTCAGCACCAACTCTGTCCTTGCCTCTGTCACAGCCTCGCTCAAG GAGCACCCCCGGGGCACCCTCCTGTCCGATGGCAGCCCGGCCCTGTCCAGGAATGTCGGTATGACGGTC TCTCAGAAAGGGGGTCCCCAGCCAACACCGAGCCCGGCTGGCCCTGGGACGCAACTCGGACCAATCACA GGAGAGATGGATGAAGCCGATTCTGCGTTTTTAAAATTTAAGCAGACAGCTGATGACTCTCTGTCCCTT ACATCTCCAAACACCGAGTCCATTTTTGTAGAAGATCCCTACACCGCCTCGCTGAGGAGTGAGATTGAG TCAGACGGCCACGAGTTTGAAGCTGAGTCCTGGAGCCTCGCCGTGGATGCAGCCTACGCCAAGAAGCAA AAGAGGGAGGTGGTGAAAAGACAAGATGTCCTTTATGAGCTGATGCAGACAGAGGTGCACCACGTGCGG ACGCTCAAGATCATGCTGAAGGTGTACTCCAGGGCCCTGCAGGAGGAGCTGCAGTTCAGCAGCAAGGCC ATTGGCCGCCTCTTCCCATGCGCTGACGACCTGCTGGAGACGCACAGCCACTTCCTCGCTCGGCTCAAG GAGCGCCGCCAGGAGTCCCTGGAGGAGGGCAGTGACCGGAATTATGTCATCCAGAAAATCGGCGACCTC CTGGTTCAGCAGTTTTCAGGTGAAAATGGGGAGAGAATGAAAGAAAAGTACGGTGTGTTTTGTAGTGGC CACAATGAAGCTGTTAGTCATTACAAGTTGCTGCTTCAGCAAAACAAGAAATTTCAAAACTTGATCAAG AAAATTGGCAACTTCTCCATCGTGCGGCGGCTTGGCGTGCAGGAGTGCATTCTCCTGGTTACACAACGC ATAACCAAATACCCAGTGCTGGTGGAGCGCATCATCCAGAACACGGAAGCTGGCACTGAGGACTATGAA GACCTGACCCAGGCCTTGAACCTCATCAAAGATATCATCTCACAAGTGGACGCCAAGGTCAGTGAGTGT GAGAAGGGCCAGCGCCTCAGGGAGATCGCAGGGAAGATGGACCTGAAGTCTTCCAGCAAACTCAAGAAC GGGCTCACCTTCCGCAAGGAAGACATGCTTCAGCGGCAGCTCCACCTGGAGGGCATGCTATGCTGGAAG ACCACATCAGGGCGCTTGAAAGATATCCTGGCTATCCTGCTGACCGACGTACTTTTGCTGCTACAAGAA AAAGATCAGAAATACGTCTTTGCTTCTGTGGACTCAAAGCCACCCGTCATCTCGTTACAAAAGCTCATC GTGAGGGAAGTGGCCAACGAGGAGAAAGCGATGTTTCTGATCAGCGCCTCCTTGCAAGGGCCGGAGATG TATGAAATCTACACGAGCTCCAAAGAGGACAGGAACGCCTGGATGGCCCACATCCAAAGGGCTGTGGAG AGCTGCCCTGACGAGGAGGAGGGGCCCTTCAGCCTGCCCGAAGAGGAAAGGAAGGTGGTCGAGGCCCGC GCCACGAGACTCCGGGACTTTCAAGAGCGGTTGAGCATGAAAGACCAGCTGATCGCACAGAGCCTCCTA GAGAAACAGCAGATCTACCTGGAGATGGCCGAGATGGGCGGCCTCGAAGACCTGCCCCAGCCCCGAGGC CTATTCCGTGGAGGGGACCCATCCGAGACCCTGCAGGGGGAGCTAATTCTCAAGTCGGCCATGAGCGAG ATCGAGGGCATCCAGAGCCTGATCTGCAGGCAGCTGGGCAGCGCCAACGGCCAGGCGGAAGACGGAGGC AGCTCCACAGGCCCGCCCAGGAGGGCTGAGACCTTCGCGGGCTACGACTGCACAAACAGCCCCACCAAG AATGGCAGTTTCAAGAAGAAAGTCAGCAGCACTGACCCCAGGCCCCGAGACTGGCGAGGCCCCCCAAAC AGCCCGGACTTGAAGCTCAGTGACAGTGACATTCCTGGGAGCTCTGAGGAATCGCCGCAGGTGGTGGAG GCGCCAGGCACGGAATCCGATCCCCGTCTGCCCACCGTCCTGGAGTCGGAGCTTGTCCAGCGGATCCAG ACACTGTCCCAGCTGCTCCTGAACCTTCAGGCGGTAATCGCCCACCAGGACAGCTATGTGGAGACGCAG CGGGCTGCCATCCAGGAGCGGGAGAAGCAGTTCCGGCTGCAGTCGACGCGTGGGAACCTGCTGCTGGAG CAGGAGCGGCAACGCAACTTCGAGAAGCAGCGGGAGGAGCGCGCGGCCCTGGAGAAGCTGCAGAGCCAG CTGCGGCACGAGCAGCAGCGCTGGGAGCGCGAGCGCCAGTGGCAGCACCAGGAGCTGGAGCGTGCGGGC GCGCGGCTGCAGGAGCGCGAGGGCGAGGCGCGGCAGCTACGCGAGCGGCTGGAGCAGGAGCGGGCCGAG CTGGAGCGCCAGCGCCAGGCCTACCAGCACGACCTGGAGCGGCTGCGCGAGGCCCAGCGTGCCGTGGAG CGCGAGCGGGAGCGCCTGGAGCTGCTGCGCCGCCTCAAGAAGCAGAACACCGCGCCAGGCGCGCTGCCG CCCGACACACTGGCCGAGGCCCAGCCCCCAAGCCACCCTCCCAGCTTCAACGGGGAAGGGCTGGAGGGC CCTCGTGTGAGCATGCTGCCATCCGGCGTGGGGCCAGAGTACGCAGAGCGCCCCGAGGTGGCTCGCCGG GACAGCGCCCCCACCGAGAACCGGCTGGCCAAGAGCGATGTGCCCATCCAGCTGCTCAGCGCCACCAAC CAGTTCCAGAGGCAGGCGGCCGTGCAGCAGCAGATCCCCACCAAGCTGGCGGCCTCCACCAAGGGTGGC AAGGACAAGGGCGGCAAGAGCAGGGGCTCTCAGCGCTGGGAGAGCTCAGCGTCCTTCGACCTGAAGCAG CAGCTGCTGCTCAACAAGCTCATGGGGAAAGATGAGAGCACCTCACGGAACCGCCGCTCGCTGAGCCCT ATCCTGCCCGGCAGACACAGTCCTGCGCCCCCACCAGACCCTGGCTTCCCCGCCCCGAGCCCACCGCCA GCTGACAGCCCCTCCGAGGGCTTCTCTCTCAAGGCCGGGGGCACAGCCCTCCTGCCCGGGCCCCCAGCT CCCTCGCCACTGCCGGCCACACCACTCAGCGCCAAGGAGGACGCCAGCAAAGAAGACGTCATCTTCTTC TAA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-RsrII |
| ACCN | NM_001130955 |
| Insert Size | 3522 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001130955.1 |
| RefSeq Size | 5773 bp |
| RefSeq ORF | 3522 bp |
| Locus ID | 23370 |
| UniProt ID | Q6ZSZ5 |
| MW | 130.8 kDa |
| Gene Summary | Rho GTPases are GTP binding proteins that regulate a wide spectrum of cellular functions. These cellular processes include cytoskeletal rearrangements, gene transcription, cell growth and motility. Activation of Rho GTPases is under the direct control of guanine nucleotide exchange factors (GEFs). The protein encoded by this gene is a guanine nucleotide exchange factor and belongs to the Rho GTPase GEF family. Family members share a common feature, a Dbl (DH) homology domain followed by a pleckstrin (PH) homology domain. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Nov 2018] Transcript Variant: This variant (2) differs in the 5' UTR and the 5' coding region, compared to variant 1. The resulting isoform (b) contains a distinct N-terminus, compared to isoform a. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC226350 | ARHGEF18 (Myc-DDK-tagged)-Human Rho/Rac guanine nucleotide exchange factor (GEF) 18 (ARHGEF18), transcript variant 2 |
CNY 8,168.00 |
|
| RC226350L3 | Lenti ORF clone of Human Rho/Rac guanine nucleotide exchange factor (GEF) 18 (ARHGEF18), transcript variant 2, Myc-DDK-tagged |
CNY 13,590.00 |
|
| RC226350L4 | Lenti ORF clone of Human Rho/Rac guanine nucleotide exchange factor (GEF) 18 (ARHGEF18), transcript variant 2, mGFP tagged |
CNY 13,590.00 |
|
| RG226350 | ARHGEF18 (tGFP-tagged) - Human Rho/Rac guanine nucleotide exchange factor (GEF) 18 (ARHGEF18), transcript variant 2 |
CNY 12,830.00 |
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