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p114RhoGEF (ARHGEF18) (NM_001130955) Human Tagged ORF Clone
CAT＃: RG226350
- TrueORF®
ARHGEF18 (tGFP-tagged) - Human Rho/Rac guanine nucleotide exchange factor (GEF) 18 (ARHGEF18), transcript variant 2
ORF Plasmid: DDK
"NM_001130955" in other vectors (4)
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CNY 12,830.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | P114-RhoGEF; p114RhoGEF; RP78; SA-RhoGEF |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG226350 representing NM_001130955
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTCACAGTGGGGACCAATATCCTGCCCTCCAGACCCGCTGCTTCAGCCAATACAGCAAGGGAAGACG CAGCTCTGTTTTCTAGAAGGATCCCACCGAGGCATAAAAACGGCGCAGCCCAGCCTGGCGCCGCGCCGGG TCCCGGAGCCCCGGGCGCGAACATGGGGAATGCGCACTCCAAAAGCGGGGACAGGCACAGCGCGCTCCCC GGCCGCCCCGAGCTGTCTTTTTACGGCTCTTTCCCCAGGAAATGGAGCGAGAACGTCTTCTTGGATAACG AGCTGCTGACCTCCAAGATCCTGTCTGTGCTGCGGCCGCAGTCGGAGCGGGGCTTCCGCGCCGGGGACCT CCGCTACCCGACCCACTTTCTCAGCACCAACTCTGTCCTTGCCTCTGTCACAGCCTCGCTCAAGGAGCAC CCCCGGGGCACCCTCCTGTCCGATGGCAGCCCGGCCCTGTCCAGGAATGTCGGTATGACGGTCTCTCAGA AAGGGGGTCCCCAGCCAACACCGAGCCCGGCTGGCCCTGGGACGCAACTCGGACCAATCACAGGAGAGAT GGATGAAGCCGATTCTGCGTTTTTAAAATTTAAGCAGACAGCTGATGACTCTCTGTCCCTTACATCTCCA AACACCGAGTCCATTTTTGTAGAAGATCCCTACACCGCCTCGCTGAGGAGTGAGATTGAGTCAGACGGCC ACGAGTTTGAAGCTGAGTCCTGGAGCCTCGCCGTGGATGCAGCCTACGCCAAGAAGCAAAAGAGGGAGGT GGTGAAAAGACAAGATGTCCTTTATGAGCTGATGCAGACAGAGGTGCACCACGTGCGGACGCTCAAGATC ATGCTGAAGGTGTACTCCAGGGCCCTGCAGGAGGAGCTGCAGTTCAGCAGCAAGGCCATTGGCCGCCTCT TCCCATGCGCTGACGACCTGCTGGAGACGCACAGCCACTTCCTCGCTCGGCTCAAGGAGCGCCGCCAGGA GTCCCTGGAGGAGGGCAGTGACCGGAATTATGTCATCCAGAAAATCGGCGACCTCCTGGTTCAGCAGTTT TCAGGTGAAAATGGGGAGAGAATGAAAGAAAAGTACGGTGTGTTTTGTAGTGGCCACAATGAAGCTGTTA GTCATTACAAGTTGCTGCTTCAGCAAAACAAGAAATTTCAAAACTTGATCAAGAAAATTGGCAACTTCTC CATCGTGCGGCGGCTTGGCGTGCAGGAGTGCATTCTCCTGGTTACACAACGCATAACCAAATACCCAGTG CTGGTGGAGCGCATCATCCAGAACACGGAAGCTGGCACTGAGGACTATGAAGACCTGACCCAGGCCTTGA ACCTCATCAAAGATATCATCTCACAAGTGGACGCCAAGGTCAGTGAGTGTGAGAAGGGCCAGCGCCTCAG GGAGATCGCAGGGAAGATGGACCTGAAGTCTTCCAGCAAACTCAAGAACGGGCTCACCTTCCGCAAGGAA GACATGCTTCAGCGGCAGCTCCACCTGGAGGGCATGCTATGCTGGAAGACCACATCAGGGCGCTTGAAAG ATATCCTGGCTATCCTGCTGACCGACGTACTTTTGCTGCTACAAGAAAAAGATCAGAAATACGTCTTTGC TTCTGTGGACTCAAAGCCACCCGTCATCTCGTTACAAAAGCTCATCGTGAGGGAAGTGGCCAACGAGGAG AAAGCGATGTTTCTGATCAGCGCCTCCTTGCAAGGGCCGGAGATGTATGAAATCTACACGAGCTCCAAAG AGGACAGGAACGCCTGGATGGCCCACATCCAAAGGGCTGTGGAGAGCTGCCCTGACGAGGAGGAGGGGCC CTTCAGCCTGCCCGAAGAGGAAAGGAAGGTGGTCGAGGCCCGCGCCACGAGACTCCGGGACTTTCAAGAG CGGTTGAGCATGAAAGACCAGCTGATCGCACAGAGCCTCCTAGAGAAACAGCAGATCTACCTGGAGATGG CCGAGATGGGCGGCCTCGAAGACCTGCCCCAGCCCCGAGGCCTATTCCGTGGAGGGGACCCATCCGAGAC CCTGCAGGGGGAGCTAATTCTCAAGTCGGCCATGAGCGAGATCGAGGGCATCCAGAGCCTGATCTGCAGG CAGCTGGGCAGCGCCAACGGCCAGGCGGAAGACGGAGGCAGCTCCACAGGCCCGCCCAGGAGGGCTGAGA CCTTCGCGGGCTACGACTGCACAAACAGCCCCACCAAGAATGGCAGTTTCAAGAAGAAAGTCAGCAGCAC TGACCCCAGGCCCCGAGACTGGCGAGGCCCCCCAAACAGCCCGGACTTGAAGCTCAGTGACAGTGACATT CCTGGGAGCTCTGAGGAATCGCCGCAGGTGGTGGAGGCGCCAGGCACGGAATCCGATCCCCGTCTGCCCA CCGTCCTGGAGTCGGAGCTTGTCCAGCGGATCCAGACACTGTCCCAGCTGCTCCTGAACCTTCAGGCGGT AATCGCCCACCAGGACAGCTATGTGGAGACGCAGCGGGCTGCCATCCAGGAGCGGGAGAAGCAGTTCCGG CTGCAGTCGACGCGTGGGAACCTGCTGCTGGAGCAGGAGCGGCAACGCAACTTCGAGAAGCAGCGGGAGG AGCGCGCGGCCCTGGAGAAGCTGCAGAGCCAGCTGCGGCACGAGCAGCAGCGCTGGGAGCGCGAGCGCCA GTGGCAGCACCAGGAGCTGGAGCGTGCGGGCGCGCGGCTGCAGGAGCGCGAGGGCGAGGCGCGGCAGCTA CGCGAGCGGCTGGAGCAGGAGCGGGCCGAGCTGGAGCGCCAGCGCCAGGCCTACCAGCACGACCTGGAGC GGCTGCGCGAGGCCCAGCGTGCCGTGGAGCGCGAGCGGGAGCGCCTGGAGCTGCTGCGCCGCCTCAAGAA GCAGAACACCGCGCCAGGCGCGCTGCCGCCCGACACACTGGCCGAGGCCCAGCCCCCAAGCCACCCTCCC AGCTTCAACGGGGAAGGGCTGGAGGGCCCTCGTGTGAGCATGCTGCCATCCGGCGTGGGGCCAGAGTACG CAGAGCGCCCCGAGGTGGCTCGCCGGGACAGCGCCCCCACCGAGAACCGGCTGGCCAAGAGCGATGTGCC CATCCAGCTGCTCAGCGCCACCAACCAGTTCCAGAGGCAGGCGGCCGTGCAGCAGCAGATCCCCACCAAG CTGGCGGCCTCCACCAAGGGTGGCAAGGACAAGGGCGGCAAGAGCAGGGGCTCTCAGCGCTGGGAGAGCT CAGCGTCCTTCGACCTGAAGCAGCAGCTGCTGCTCAACAAGCTCATGGGGAAAGATGAGAGCACCTCACG GAACCGCCGCTCGCTGAGCCCTATCCTGCCCGGCAGACACAGTCCTGCGCCCCCACCAGACCCTGGCTTC CCCGCCCCGAGCCCACCGCCAGCTGACAGCCCCTCCGAGGGCTTCTCTCTCAAGGCCGGGGGCACAGCCC TCCTGCCCGGGCCCCCAGCTCCCTCGCCACTGCCGGCCACACCACTCAGCGCCAAGGAGGACGCCAGCAA AGAAGACGTCATCTTCTTC AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG226350 representing NM_001130955
Red=Cloning site Green=Tags(s) MVTVGTNILPSRPAASANTAREDAALFSRRIPPRHKNGAAQPGAAPGPGAPGANMGNAHSKSGDRHSALP GRPELSFYGSFPRKWSENVFLDNELLTSKILSVLRPQSERGFRAGDLRYPTHFLSTNSVLASVTASLKEH PRGTLLSDGSPALSRNVGMTVSQKGGPQPTPSPAGPGTQLGPITGEMDEADSAFLKFKQTADDSLSLTSP NTESIFVEDPYTASLRSEIESDGHEFEAESWSLAVDAAYAKKQKREVVKRQDVLYELMQTEVHHVRTLKI MLKVYSRALQEELQFSSKAIGRLFPCADDLLETHSHFLARLKERRQESLEEGSDRNYVIQKIGDLLVQQF SGENGERMKEKYGVFCSGHNEAVSHYKLLLQQNKKFQNLIKKIGNFSIVRRLGVQECILLVTQRITKYPV LVERIIQNTEAGTEDYEDLTQALNLIKDIISQVDAKVSECEKGQRLREIAGKMDLKSSSKLKNGLTFRKE DMLQRQLHLEGMLCWKTTSGRLKDILAILLTDVLLLLQEKDQKYVFASVDSKPPVISLQKLIVREVANEE KAMFLISASLQGPEMYEIYTSSKEDRNAWMAHIQRAVESCPDEEEGPFSLPEEERKVVEARATRLRDFQE RLSMKDQLIAQSLLEKQQIYLEMAEMGGLEDLPQPRGLFRGGDPSETLQGELILKSAMSEIEGIQSLICR QLGSANGQAEDGGSSTGPPRRAETFAGYDCTNSPTKNGSFKKKVSSTDPRPRDWRGPPNSPDLKLSDSDI PGSSEESPQVVEAPGTESDPRLPTVLESELVQRIQTLSQLLLNLQAVIAHQDSYVETQRAAIQEREKQFR LQSTRGNLLLEQERQRNFEKQREERAALEKLQSQLRHEQQRWERERQWQHQELERAGARLQEREGEARQL RERLEQERAELERQRQAYQHDLERLREAQRAVERERERLELLRRLKKQNTAPGALPPDTLAEAQPPSHPP SFNGEGLEGPRVSMLPSGVGPEYAERPEVARRDSAPTENRLAKSDVPIQLLSATNQFQRQAAVQQQIPTK LAASTKGGKDKGGKSRGSQRWESSASFDLKQQLLLNKLMGKDESTSRNRRSLSPILPGRHSPAPPPDPGF PAPSPPPADSPSEGFSLKAGGTALLPGPPAPSPLPATPLSAKEDASKEDVIFF SGPTRTRRLE - GFP Tag - V |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001130955 |
| ORF Size | 3519 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001130955.1, NP_001124427.1 |
| RefSeq Size | 5773 bp |
| RefSeq ORF | 3360 bp |
| Locus ID | 23370 |
| UniProt ID | Q6ZSZ5 |
| Gene Summary | Rho GTPases are GTP binding proteins that regulate a wide spectrum of cellular functions. These cellular processes include cytoskeletal rearrangements, gene transcription, cell growth and motility. Activation of Rho GTPases is under the direct control of guanine nucleotide exchange factors (GEFs). The protein encoded by this gene is a guanine nucleotide exchange factor and belongs to the Rho GTPase GEF family. Family members share a common feature, a Dbl (DH) homology domain followed by a pleckstrin (PH) homology domain. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Nov 2018] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC226350 | ARHGEF18 (Myc-DDK-tagged)-Human Rho/Rac guanine nucleotide exchange factor (GEF) 18 (ARHGEF18), transcript variant 2 |
CNY 8,168.00 |
|
| RC226350L3 | Lenti ORF clone of Human Rho/Rac guanine nucleotide exchange factor (GEF) 18 (ARHGEF18), transcript variant 2, Myc-DDK-tagged |
CNY 13,590.00 |
|
| RC226350L4 | Lenti ORF clone of Human Rho/Rac guanine nucleotide exchange factor (GEF) 18 (ARHGEF18), transcript variant 2, mGFP tagged |
CNY 13,590.00 |
|
| SC326348 | ARHGEF18 (untagged)-Human Rho/Rac guanine nucleotide exchange factor (GEF) 18 (ARHGEF18), transcript variant 2 |
CNY 22,520.00 |
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