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PDE10A (NM_001130690) Human Untagged Clone
CAT＃: SC326233
PDE10A (untagged)-Human phosphodiesterase 10A (PDE10A), transcript variant 1
CN¥ 15,200.00
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Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ADSD2; HSPDE10A; IOLOD; LINC00473; PDE10A19 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_001130690, the custom clone sequence may differ by one or more nucleotides
ATGGAAGATGGACCTTCTAATAATGCGAGCTGCTTCCGAAGGCTGACCGAGTGCTTCCTGAGCCCCAGTT TGACAGATGAAAAAGTGAAGGCATATCTTTCTCTTCACCCCCAGGTATTAGATGAATTTGTATCTGAAAG TGTTAGTGCAGAGACAGTAGAGAAATGGCTGAAGAGGAAGAACAACAAATCAGAAGATGAATCAGCTCCT AAGGAAGTCAGCAGGTACCAAGATACGAATATGCAGGGAGTTGTATATGAACTAAACAGCTATATAGAAC AACGGTTGGACACAGGAGGAGACAACCAGCTACTCCTCTATGAACTGAGCAGCATCATTAAAATAGCCAC AAAAGCCGATGGATTTGCACTGTATTTCCTTGGAGAGTGCAATAATAGCCTGTGTATATTCACGCCACCT GGGATAAAGGAAGGAAAACCCCGCCTCATCCCTGCTGGGCCCATCACTCAGGGCACCACCGTCTCTGCTT ATGTGGCCAAGTCCAGGAAAACACTGCTAGTAGAAGACATCCTTGGAGATGAACGATTTCCAAGAGGTAC TGGACTGGAATCAGGGACTCGTATCCAGTCTGTTCTTTGCTTACCAATTGTCACTGCAATTGGTGACTTG ATTGGTATTCTCGAGCTGTATCGGCACTGGGGCAAAGAAGCCTTCTGTCTTAGTCACCAGGAGGTTGCAA CAGCAAATCTTGCCTGGGCTTCAGTAGCAATACATCAGGTGCAGGTATGCAGAGGCCTTGCCAAACAGAC AGAATTGAATGACTTCCTACTCGACGTATCAAAAACATATTTTGATAACATAGTTGCAATAGATTCTCTA CTTGAACACATAATGATATATGCAAAAAACCTGGTGAATGCCGATCGTTGTGCGCTTTTCCAGGTGGACC ATAAGAACAAGGAGTTATATTCAGACCTTTTTGATATTGGAGAGGAAAAGGAAGGAAAACCTGTCTTCAA GAAGACCAAAGAGATAAGATTTTCAATTGAGAAAGGAATTGCTGGCCAAGTAGCAAGAACAGGGGAAGTC CTGAACATTCCAGATGCCTATGCAGACCCACGCTTTAACAGAGAAGTAGACTTGTACACAGGCTACACCA CGCGGAACATCCTGTGCATGCCCATCGTCAGCCGAGGCAGCGTGATAGGTGTGGTGCAGATGGTCAACAA AATCAGTGGCAGTGCCTTCTCTAAAACAGATGAAAACAACTTCAAAATGTTTGCCGTCTTTTGTGCTTTA GCCTTACACTGTGCTAATATGTATCATAGAATTCGCCACTCAGAGTGCATTTACCGGGTAACGATGGAAA AGCTGTCCTACCATAGCATTTGTACTTCAGAAGAGTGGCAAGGTCTCATGCAATTCACCCTTCCCGTGCG TCTCTGCAAAGAAATTGAATTATTCCACTTTGACATTGGTCCTTTTGAAAACATGTGGCCTGGAATTTTT GTCTACATGGTTCATCGGTCCTGTGGGACATCCTGCTTTGAGCTTGAAAAGTTGTGTCGTTTTATTATGT CTGTGAAGAAGAACTATCGGCGGGTTCCTTATCACAACTGGAAGCATGCGGTCACTGTAGCACACTGCAT GTATGCCATACTTCAGAACAATCACACGCTTTTCACAGACCTTGAGCGCAAAGGACTGCTGATTGCGTGT CTGTGTCATGACCTGGACCACAGGGGCTTCAGTAACAGCTACCTGCAGAAGTTCGACCACCCTCTGGCCG CTCTCTACTCCACTTCCACCATGGAGCAGCACCACTTCTCCCAGACTGTGTCCATCCTCCAGTTGGAAGG GCACAATATCTTCTCCACTCTGAGCTCCAGTGAATATGAGCAGGTGCTTGAGATCATCCGCAAAGCCATC ATTGCCACAGACCTTGCTTTATACTTTGGAAACAGGAAGCAGTTGGAAGAGATGTACCAGACCGGATCAC TAAACCTTAATAATCAATCACATAGAGACCGTGTAATTGGTTTGATGATGACTGCCTGTGACCTTTGTTC TGTGACAAAACTGTGGCCCGTTACAAAATTGACGGCAAATGATATATATGCAGAATTCTGGGCTGAGGGT GATGAAATGAAGAAATTGGGAATACAGCCTATTCCTATGATGGACAGAGACAAGAAGGATGAAGTCCCCC AAGGCCAGCTTGGGTTCTACAATGCCGTGGCCATTCCCTGCTATACAACCCTTACCCAGATCCTCCCTCC CACGGAGCCTCTTCTGAAAGCATGCAGGGATAATCTCAGTCAGTGGGAGAAGGTGATTCGAGGGGAGGAG ACTGCAACCTGGATTTCATCCCCATCCGTGGCTCAGAAGGCAGCTGCATCTGAAGATTGA |
| Restriction Sites | Please inquire |
| ACCN | NM_001130690 |
| Insert Size | 2600 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001130690.1, NP_001124162.1 |
| RefSeq Size | 8207 bp |
| RefSeq ORF | 2370 bp |
| Locus ID | 10846 |
| UniProt ID | Q9Y233 |
| Protein Families | Druggable Genome |
| Protein Pathways | Progesterone-mediated oocyte maturation, Purine metabolism |
| Gene Summary | The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase family. It plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This protein can hydrolyze both cAMP and cGMP to the corresponding nucleoside 5' monophosphate, but has higher affinity for cAMP, and is more efficient with cAMP as substrate. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Dec 2011] Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC226169 | PDE10A (Myc-DDK-tagged)-Human phosphodiesterase 10A (PDE10A), transcript variant 1 |
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| RC226169L1 | Lenti-ORF clone of PDE10A (Myc-DDK-tagged)-Human phosphodiesterase 10A (PDE10A), transcript variant 1 |
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| RC226169L2 | Lenti-ORF clone of PDE10A (mGFP-tagged)-Human phosphodiesterase 10A (PDE10A), transcript variant 1 |
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| RC226169L3 | Lenti-ORF clone of PDE10A (Myc-DDK-tagged)-Human phosphodiesterase 10A (PDE10A), transcript variant 1 |
CN¥ 10,260.00 |
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| RC226169L4 | Lenti-ORF clone of PDE10A (mGFP-tagged)-Human phosphodiesterase 10A (PDE10A), transcript variant 1 |
CN¥ 10,260.00 |
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| RG226169 | PDE10A (tGFP-tagged) - Human phosphodiesterase 10A (PDE10A), transcript variant 1 |
CN¥ 9,220.00 |
