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Apc5 (ANAPC5) (NM_001137559) Human Untagged Clone
CAT＃: SC326144
ANAPC5 (untagged)-Human anaphase promoting complex subunit 5 (ANAPC5), transcript variant 2
CNY 10,360.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | APC5 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC326144 representing NM_001137559.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCTGAAGGCGAGTTGAAGGATATGGAACAGTTTTTTGATGACCTTTCAGATTCTTTCTCTGGAACT GAACCAGAGGTTCACAAAACAAGTGTAGTAGGTTTGTTTCTGCGTCACATGATCTTGGCCTACAGTAAG CTTTCTTTCAGCCAAGTGTTTAAACTGTACACTGCCCTTCAGCAGTACTTCCAGAATGGTGAGAAAAAG ACAGTGGAGGATGCTGATATGGAACTGACCAGTAGAGATGAGGGTGAAAGAAAAATGGAAAAAGAAGAA CTTGATGTATCTGTAAGAGAAGAGGAGGTATCTTGCAGTGGGCCTCTGTCCCAAAAACAAGCAGAATTT TTTCTTTCTCAACAGGCTTCTTTGCTAAAGAATGATGAGACTAAGGCCCTCACTCCAGCTTCCTTGCAG AAGGAATTAAACAATTTGTTGAAATTTAATCCTGATTTTGCTGAAGCGCATTATCTCAGCTACTTAAAC AACCTCCGTGTCCAAGATGTTTTCAGTTCAACACACAGTCTCCTCCATTATTTTGATCGTCTGATTCTT ACCGGAGCCGAAAGCAAAAGTAATGGGGAAGAGGGCTATGGCCGGAGCTTGAGATACGCCGCTCTGAAT CTTGCCGCCCTGCACTGCCGCTTCGGTCACTATCAACAGGCAGAGCTCGCCCTGCAGGAGGCAATTAGG ATTGCCCAGGAGTCCAACGATCACGTGTGTCTCCAGCACTGTTTGAGCTGGCTTTATGTGCTGGGGCAG AAGAGATCCGATAGCTATGTTCTGCTGGAGCATTCTGTGAAGAAGGCAGTACATTTTGGGTTACCGAGA GCTTTTGCTGGGAAGACGGCAAACAAGCTGATGGATGCCCTAAAGGACTCCGACCTCCTGCACTGGAAA CACAGCCTGTCAGAGCTCATCGATATCAGCATCGCACAGAAAACGGCCATCTGGAGGCTGTATGGCCGC AGCACCATGGCACTGCAACAGGCCCAGATGTTGCTGAGCATGAACAGCCTGGAGGCGGTGAATGCGGGC GTGCAGCAGAACAACACAGAGTCCTTTGCTGTCGCACTCTGCCACCTCGCAGAGCTACACGCGGAGCAG GGCTGTTTTGCTGCAGCTTCTGAAGTGTTAAAGCACTTGAAGGAACGATTTCCGCCTAATAGTCAGCAC GCCCAGTTATGGATGCTATGTGATCAAAAAATACAGTTTGACAGAGCAATGAATGATGGCAAATATCAT TTGGCTGATTCACTTGTTACAGGAATCACAGCTCTCAATAGCATAGAGGGTGTTTATAGGAAAGCGGTT GTATTACAAGCTCAGAACCAAATGTCAGAGGCACATAAGCTTTTACAAAAATTGTTGGTTCATTGTCAG AAACTGAAGAACACAGAAATGGTGATCAGTGTCCTACTGTCCGTGGCAGAGCTGTACTGGCGATCTTCC TCCCCTACCATCGCGCTGCCCATGCTCCTGCAGGCTCTGGCCCTCTCCAAGGAGTACCGGTTACAGTAC TTGGCCTCTGAAACAGTGCTGAACTTGGCTTTTGCGCAGCTCATTCTTGGAATCCCAGAACAGGCCTTA AGTCTTCTCCACATGGCCATCGAGCCCATCTTGGCTGACGGGGCTATCCTGGACAAAGGTCGTGCCATG TTCTTAGTGGCCAAGTGCCAGGTGGCTTCAGCAGCTTCCTACGATCAGCCGAAGAAAGCAGAAGCTCTG GAGGCTGCCATCGAGAACCTCAATGAAGCCAAGAACTATTTTGCAAAGGTTGACTGCAAAGAGCGCATC AGGGACGTCGTTTACTTCCAGGCCAGACTCTACCATACCCTGGGGAAGACCCAGGAGAGGAACCGGTGT GCGATGCTCTTCCGGCAGCTGCATCAGGAGCTGCCCTCTCATGGGGTACCCTTGATAAACCATCTCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001137559 |
| Insert Size | 1932 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001137559.1 |
| RefSeq Size | 2364 bp |
| RefSeq ORF | 1932 bp |
| Locus ID | 51433 |
| UniProt ID | Q9UJX4 |
| Protein Families | Druggable Genome |
| Protein Pathways | Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation, Ubiquitin mediated proteolysis |
| MW | 72.5 kDa |
| Gene Summary | This gene encodes a tetratricopeptide repeat-containing component of the anaphase promoting complex/cyclosome (APC/C), a large E3 ubiquitin ligase that controls cell cycle progression by targeting a number of cell cycle regulators such as B-type cyclins for 26S proteasome-mediated degradation through ubiquitination. The encoded protein is required for the proper ubiquitination function of APC/C and for the interaction of APC/C with transcription coactivators. It also interacts with polyA binding protein and represses internal ribosome entry site-mediated translation. Multiple transcript variants encoding different isoforms have been found for this gene. These differences cause translation initiation at a downstream AUG and result in a shorter protein (isoform b), compared to isoform a. [provided by RefSeq, Nov 2008] Transcript Variant: This variant (2) contains a distinct 5' UTR and lacks an in-frame portion of the internal coding region, compared to variant 1. These differences cause translation initiation at a downstream AUG and result in a shorter isoform (b) compared to isoform a. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC226883 | ANAPC5 (Myc-DDK-tagged)-Human anaphase promoting complex subunit 5 (ANAPC5), transcript variant 2 |
CNY 4,784.00 |
|
| RC226883L3 | Lenti-ORF clone of ANAPC5 (Myc-DDK-tagged)-Human anaphase promoting complex subunit 5 (ANAPC5), transcript variant 2 |
CNY 7,600.00 |
|
| RC226883L4 | Lenti-ORF clone of ANAPC5 (mGFP-tagged)-Human anaphase promoting complex subunit 5 (ANAPC5), transcript variant 2 |
CNY 7,600.00 |
|
| RG226883 | ANAPC5 (tGFP-tagged) - Human anaphase promoting complex subunit 5 (ANAPC5), transcript variant 2 |
CNY 6,270.00 |
