United States
Germany
Japan
United Kingdom
China
CoCoA (CALCOCO1) (NM_001143682) Human Untagged Clone
CAT＃: SC326102
CALCOCO1 (untagged)-Human calcium binding and coiled-coil domain 1 (CALCOCO1), transcript variant 2
CNY 9,790.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | calphoglin; Cocoa; PP13275 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC326102 representing NM_001143682.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGAAGAATCACCACTAAGCCGGGCACCATCCCGTGGTGGAGTCAACTTTCTCAATGTAGCCCGGACC TACATCCCCAACACCAAGGTGGAATGTCACTACACCCTTCCCCCAGGCACCATGCCCAGTGCCAGTGAC TGGATTGGCATCTTCAAGGTGGAGGCTGCCTGTGTTCGGGATTACCACACATTTGTGTGGTCTTCCGTG CCTGAAAGTACAACTGATGGTTCCCCCATTCACACCAGTGTCCAGTTCCAAGAGCCAAGGCCCATGGAT GAACTGGTGACCCTGGAGGAGGCTGATGGGGGCTCTGACATCCTGCTGGTTGTCCCCAAGGCAACTGTG TTACAGAACCAGCTCGATGAGAGCCAGCAAGAACGGAATGACCTGATGCAGCTGAAGCTACAGCTGGAG GGACAGGTGACAGAGCTGAGGAGCCGAGTGCAGGAGCTCGAGAGGGCTCTGGCAACTGCCAGGCAGGAG CACACGGAGCTGATGGAACAGTACAAGGGGATTTCCCGGTCCCATGGGGAGATCACAGAAGAGAGGGAC ATCCTGAGCCGGCAACAGGGAGACCATGTGGCACGCATCCTGGAGCTAGAGGATGACATCCAGACCATC AGTGAGAAAGTGCTGACGAAGGAAGTGGAGCTGGACAGGCTTAGAGACACAGTGAAGGCCCTGACTCGG GAACAAGAGAAGCTCCTTGGGCAACTGAAAGAAGTACAAGCAGACAAGGAGCAAAGTGAGGCCGAGCTG GAGCCCTTGAAGGAGCAGCTTCGAGGGGCCCAGGAGCTTGCAGCCTCAAGCCAGCAGAAAGCCACCCTT CTTGGGGAGGAGTTGGCCAGTGCAGCAGCAGCCAGGGACCGCACCATAGCCGAACTACACCGCAGCCGC CTGGAAGTGGCTGAAGTTAACGGCAGGCTGGCTGAGCTCGGTTTGCACTTGAAGGAAGAAAAATGCCAA TGGAGCAAGGAGCGGGCAGGGCTGCTGCAGAGTGTGGAGGCAGAGAAGGACAAGATCCTGAAGCTGAGT GCAGAGATACTTCGATTGGAGAAGGCAGTTCAGGAGGAGAGGACCCAAAACCAAGTGTTCAAGACTGAG CTGGCCCGGGAGAAGGATTCTAGCCTGGTACAGTTGTCAGAAAGTAAGCGGGAGCTGACAGAGCTGCGG TCAGCCCTGCGTGTGCTCCAGAAGGAAAAGGAGCAGTTACAGGAGGAGAAACAGGAATTGCTAGAGTAC ATGAGAAAGCTAGAGGCCCGCCTGGAGAAGGTGGCAGATGAGAAGTGGAATGAGGATGCCACCACAGAG GATGAGGAGGCCGCTGTGGGGCTGAGCTGCCCGGCAGCTCTGACAGACTCAGAGGACGAGTCCCCAGAA GACATGAGGCTCCCACCCTATGGCCTTTGTGAGCGTGGAGACCCAGGCTCCTCTCCTGCTGGGCCTCGA GAGGCTTCTCCCCTTGTTGTCATCAGCCAGCCGGCTCCCATTTCTCCTCACCTCTCTGGGCCAGCTGAG GACAGTAGCTCTGACTCGGAGGCTGAAGATGAGAAGTCAGTCCTGATGGCAGCTGTGCAGAGTGGGGGT GAGGAGGCCAACTTACTGCTTCCTGAACTGGGCAGTGCCTTCTATGACATGGCCAGTGGCTTTACAGTG GGTACCCTGTCAGAAACCAGCACTGGGGGCCCTGCCACCCCCACATGGAAGGAGTGTCCTATCTGTAAG GAGCGCTTTCCTGCTGAGAGTGACAAGGATGCCCTGGAGGACCACATGGATGGACACTTCTTTTTCAGC ACCCAGGACCCCTTCACCTTTGAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001143682 |
| Insert Size | 1821 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001143682.1 |
| RefSeq Size | 2790 bp |
| RefSeq ORF | 1821 bp |
| Locus ID | 57658 |
| UniProt ID | Q9P1Z2 |
| Protein Families | Transcription Factors |
| MW | 67.5 kDa |
| Gene Summary | Functions as a coactivator for aryl hydrocarbon and nuclear receptors (NR). Recruited to promoters through its contact with the N-terminal basic helix-loop-helix-Per-Arnt-Sim (PAS) domain of transcription factors or coactivators, such as NCOA2. During ER-activation acts synergistically in combination with other NCOA2-binding proteins, such as EP300, CREBBP and CARM1. Involved in the transcriptional activation of target genes in the Wnt/CTNNB1 pathway. Functions as a secondary coactivator in LEF1-mediated transcriptional activation via its interaction with CTNNB1. Coactivator function for nuclear receptors and LEF1/CTNNB1 involves differential utilization of two different activation regions (By similarity). In association with CCAR1 enhances GATA1- and MED1-mediated transcriptional activation from the gamma-globin promoter during erythroid differentiation of K562 erythroleukemia cells (PubMed:24245781).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) omits an inframe coding exon and uses an alternate (in-frame) splice acceptor site, compared to variant 2. This results in a shorter protein (isoform 2). |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC227956 | CALCOCO1 (Myc-DDK-tagged)-Human calcium binding and coiled-coil domain 1 (CALCOCO1), transcript variant 2 |
CNY 4,512.00 |
|
| RC227956L3 | Lenti-ORF clone of CALCOCO1 (Myc-DDK-tagged)-Human calcium binding and coiled-coil domain 1 (CALCOCO1), transcript variant 2 |
CNY 6,460.00 |
|
| RC227956L4 | Lenti-ORF clone of CALCOCO1 (mGFP-tagged)-Human calcium binding and coiled-coil domain 1 (CALCOCO1), transcript variant 2 |
CNY 6,460.00 |
|
| RG227956 | CALCOCO1 (tGFP-tagged) - Human calcium binding and coiled-coil domain 1 (CALCOCO1), transcript variant 2 |
CNY 5,040.00 |
