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GUCY1A3 (NM_001130686) Human Untagged Clone
CAT＃: SC325830
GUCY1A3 (untagged)-Human guanylate cyclase 1, soluble, alpha 3 (GUCY1A3), transcript variant 6
CNY 5,990.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | GC-SA3; GUC1A3; GUCA3; GUCSA3; GUCY1A1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC325830 representing NM_001130686.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTTCTGCACGAAGCTCAAGGATCTCAAGATCACAGGAGAGTGTCCTTTCTCCTTACTGGCACCAGGT CAAGTTCCTAACGAGTCTTCAGAGGAGGCAGCAGGAAGCTCAGAGAGCTGCAAAGCAACCGTGCCCATC TGTCAAGACATTCCTGAGAAGAACATACAAGAAAGTCTTCCTCAAAGAAAAACCAGTCGGAGCCGAGTC TATCTTCACACTTTGGCAGAGAGTATTTGCAAACTGATTTTCCCAGAGTTTGAACGGCTGAATGTTGCA CTTCAGAGAACATTGGCAAAGCACAAAATAAAAGAAAGCAGGAAATCTTTGGAAAGAGAAGACTTTGAA AAAACAATTGCAGAGCAAGCAGTTGCAGCAGGAGTTCCAGTGGAGGTTATCAAAGAATCTCTTGGTGAA GAGGTTTTTAAAATATGTTACGAGGAAGATGAAAACATCCTTGGGGTGGTTGGAGGCACCCTTAAAGAT TTTTTAAACAGCTTCAGTACCCTTCTGAAACAGAGCAGCCATTGCCAAGAAGCAGGAAAAAGGGGCAGG CTTGAGGACGCCTCCATTCTATGCCTGGATAAGGAGGATGATTTTCTACATGTTTACTACTTCTTCCCT AAGAGAACCACCTCCCTGATTCTTCCCGGCATCATAAAGGCAGCTGCTCACGTATTATATGAAACGGAA GTGGAAGTGTCGTTAATGCCTCCCTGCTTCCATAATGATTGCAGCGAGTTTGTGAATCAGCCCTACTTG TTGTACTCCGTTCACATGAAAAGCACCAAGCCATCCCTGTCCCCCAGCAAACCCCAGTCCTCGCTGGTG ATTCCCACATCGCTATTCTGCAAGACATTTCCATTCCATTTCATGTTTGACAAAGATATGACAATTCTG CAATTTGGCAATGGCATCAGAAGGCTGATGAACAGGAGAGACTTTCAAGGAAAGCCTAATTTTGAAGAA TACTTTGAAATTCTGACTCCAAAAATCAACCAGACGTTTAGCGGGATCATGACTATGTTGAATATGCAG TTTGTTGTACGAGTGAGGAGATGGGACAACTCTGTGAAAAAATCTTCAAGGGTAAGGAAAACATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001130686 |
| Insert Size | 1101 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001130686.1 |
| RefSeq Size | 1658 bp |
| RefSeq ORF | 1101 bp |
| Locus ID | 2982 |
| Protein Families | Druggable Genome |
| Protein Pathways | Gap junction, Long-term depression, Purine metabolism, Vascular smooth muscle contraction |
| MW | 41.7 kDa |
| Gene Summary | Soluble guanylate cyclases are heterodimeric proteins that catalyze the conversion of GTP to 3',5'-cyclic GMP and pyrophosphate. The protein encoded by this gene is an alpha subunit of this complex and it interacts with a beta subunit to form the guanylate cyclase enzyme, which is activated by nitric oxide. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq, Jan 2012] Transcript Variant: This variant (6) differs in the 5' UTR and uses an alternate splice site in the 3' coding region that results in a premature translation termination site, compared to variant 1. The encoded protein (isoform C) has a shorter and distinct C-terminus, compared to isoform A. |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC225555 | GUCY1A3 (Myc-DDK-tagged)-Human guanylate cyclase 1, soluble, alpha 3 (GUCY1A3), transcript variant 6 |
CNY 3,656.00 |
|
| RC225555L3 | Lenti-ORF clone of GUCY1A3 (Myc-DDK-tagged)-Human guanylate cyclase 1, soluble, alpha 3 (GUCY1A3), transcript variant 6 |
CNY 5,890.00 |
|
| RC225555L4 | Lenti-ORF clone of GUCY1A3 (mGFP-tagged)-Human guanylate cyclase 1, soluble, alpha 3 (GUCY1A3), transcript variant 6 |
CNY 5,890.00 |
|
| RG225555 | GUCY1A3 (tGFP-tagged) - Human guanylate cyclase 1, soluble, alpha 3 (GUCY1A3), transcript variant 6 |
CNY 4,370.00 |
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