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Myosin Phosphatase (PPP1R12A) (NM_001143886) Human Untagged Clone
CAT＃: SC325374
PPP1R12A (untagged)-Human protein phosphatase 1, regulatory (inhibitor) subunit 12A (PPP1R12A), transcript variant 3
CNY 6,912.00
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | GUBS; M130; MBS; MYPT1 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_001143886 edited
GGGCGGCCGCGAATTCGGCACGAGGCCCTCTCCGCCTCCCCCTTCCCCCCCTCGCGATAA GAAGACCCGGCGGCAGGAGAGGGGATGAAGATGGCGGACGCGAAGCAGAAGCGGAACGAG CAGCTGAAACGCTGGATCGGCTCCGAGACGGACCTCGAGCCTCCGGTGGTGAAGCGCCAG AAGACCAAGGTGAAGTTCGACGATGGCGCCGTCTTCCTGGCTGCTTGCTCCAGCGGCGAC ACGGACGAGGTCCTCAAGCTGCTGCACCGCGGCGCCGACATCAATTACGCCAATGTGGAC GGACTCACTGCCCTGCACCAGGCTTGCATTGATGACAATGTTGATATGGTGAAGTTTCTG GTAGAAAATGGAGCAAATATTAATCAACCTGATAATGAAGGCTGGATACCACTACATGCA GCAGCTTCCTGTGGATATCTTGATATTGCAGAGTTTTTGATTGGTCAAGGAGCACATGTA GGGGCTGTCAACAGTGAAGGAGATACACCTTTAGATATTGCGGAGGAGGAGGCAATGGAA GAGCTACTTCAAAATGAAGTTAATCGGCAAGGGGTTGATATAGAAGCAGCTCGAAAGGAA GAAGAACGGATCATGCTTAGAGATGCCAGGCAGTGGCTAAATAGTGGTCATATAAATGAT GTCCGGCATGCAAAATCTGGAGGTACAGCACTTCACGTTGCAGCTGCTAAAGGCTATACG GAAGTTTTAAAACTTTTAATACAGGCAGGCTATGATGTTAATATTAAAGACTATGATGGC TGGACACCTCTTCATGCTGCAGCTCATTGGGGTAAAGAAGAAGCATGTCGAATTTTAGTG GACAATCTGTGTGATATGGAGATGGTCAACAAAGTGGGCCAAACAGCCTTTGATGTAGCA GATGAAGACATTTTAGGATATTTAGAAGAGTTGCAAAAGAAACAAAATCTGCTCCATAGT GAAAAACGGGACAAGAAATCTCCACTAATTGAATCAACAGCAAATATGGACAATAATCAG TCACAGAAGACCTTTAAAAACAAAGAGACGTTGATTATTGAACCAGAGAAAAATGCATCC CGTATTGAATCTCTGGAACAAGAAAAGGTTGATGAAGAAGAAGAAGGAAAGAAGGATGAG TCTAGCTGCTCTAGTGAAGAAGATGAGGAAGATGACTCGGAATCAGAAGCTGAAACAGAT AAGACAAAACCCCTGGCTTCTGTAACTAATGCCAACACTTCTAGTACACAAGCAGCTCCT GTAGCTGTTACAACACCTACTGTGTCATCAGGTCAAGCAACACCTACATCACCTATTAAA AAGTTTCCAACCACAGCTACAAAAATTTCTCCCAAAGAAGAAGAGAGAAAAGATGAGTCT CCTGCAACTTGGAGGTTAGGACTTAGAAAGACGGGCAGCTATGGTGCACTTGCTGAAATC ACAGCATCTAAAGAGGGTCAGAAAGAAAAAGATACTGCAGGTGTTACACGTTCAGCTTCA AGTCCCAGACTTTCCTCCTCTTTGGATAATAAAGAAAAGGAGAAAGATAGTAAAGGAACT AGGCTTGCATATGTTGCACCTACAATACCAAGACGACTAGCCAGTACATCTGACATTGAA GAGAAAGAAAACAGAGATTCTTCAAGTTTGCGAACAAGTAGTTCATATACAAGGAGAAAA TGGGAAGATGATCTTAAAAAAAATAGCTCAGTTAATGAAGGATCAACGTATCATAAAAGT TGCTCCTTTGGTAGAAGACAAGATGATTTGATTAGTTCTAGTGTTCCAAGCACCACATCA ACACCAACAGTTACCTCTGCAGCTGGGCTTCAGAAAAGCCTGCTTTCCAGCACAAGCACT ACTACAAAGATTACAACGGGTTCTTCCTCAGCAGGCACACAAAGCAGTACCTCAAATCGT TTGTGGGCTGAGGATAGTACTGAGAAAGAAAAGGACAGTGTTCCTACGGCAGTGACCATT CCTGTTGCTCCAACTGTTGTAAATGCTGCAGCTTCTACCACAACCCTGACTACAACTACT GCTGGCACTGTCTCCTCCACAACAGAGGTCAGGGAGAGACGCAGATCATACCTCACTCCT GTTAGGGATGAAGAGTCTGAATCCCAAAGAAAAGCAAGATCTAGACAAGCAAGACAATCT AGAAGATCAACACAGGGAGTGACATTAACTGATCTTCAAGAAGCTGAGAAAACAATAGGA AGAAGTCGTTCTACCCGAACCAGAGAACAAGAAAATGAAGAAAAAGAAAAAGAGGAAAAA GAGAAACAAGATAAAGAGAAACAAGAAGAAAAGAAGGAGTCAGAAACATCTAGAGAAGAT GAATATAAACAAAAGTACTCCAGAACGTATGATGAGACTTACCAGCGTTATAGGCCAGTA TCAACTTCAAGTTCAACCACTCCATCCTCTTCACTTTCTACTATGAGCAGTTCACTGTAT GCTTCAAGTCAACTAAACAGGCCAAATAGTCTTGTAGGCATAACTTCTGCTTACTCCAGA GGAATAACAAAAGAAAATGAAAGAGAGGGAGAAAAAAGAGAAGAGGAGAAAGAAGGAGAA GATAAATCACAACCTAAATCAATCAGAGAACGACGACGACCAAGAGAGAAAAGAAGATCT ACAGGAGTTTCATTTTGGACACAAGATAGTGATGAAAATGAACAAGAACAACAATCAGAC ACAGAAGAGGGATCCAATAAGAAAGAAACTCAGACGGATTCCATTTCTAGATATGAAACC AGTTCTACATCAGCTGGTGATCGATATGATTCCTTGCTGGGTCGCTCTGGATCATACAGT TACTTAGAAGAAAGAAAACCTTACAGCAGCAGGCTAGAAAAGGATGACTCAACTGACTTT AAAAAGCTTTATGAACAAATTCTAGCTGAAAATGAAAAGCTGAAGGCACAGCTACATGAT ACAAATATGGAACTAACAGATCTTAAATTACAGTTGGAAAAGGCCACCCAGAGACAAGAA AGATTTGCTGATAGATCACTGTTGGAAATGGAAAAAAGGGAACGAAGAGCTCTAGAAAGA AGAATATCTGAAATGGAAGAAGAGCTCAAAATGTTACCAGACCTAAAAGCAGACAACCAG AGGCTAAAGGATGAAAATGGGGCCTTGATCAGAGTTATAAGCAAACTTTCCAAAAAAAAA AAAAAAAAACTCGACTCTAGATTGCGGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCG GGTGGCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAG TGCCCACCAGCCTTGTCCTAATAAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCT TCTATAATAT |
| Restriction Sites | Please inquire |
| ACCN | NM_001143886 |
| Insert Size | 3500 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001143886.1, NP_001137358.1 |
| RefSeq Size | 5337 bp |
| RefSeq ORF | 2832 bp |
| Locus ID | 4659 |
| UniProt ID | O14974 |
| Protein Families | Druggable Genome |
| Protein Pathways | Focal adhesion, Long-term potentiation, Regulation of actin cytoskeleton, Vascular smooth muscle contraction |
| Gene Summary | Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2009] Transcript Variant: This variant (3) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (b) is shorter at the N-terminus compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC227497 | PPP1R12A (Myc-DDK-tagged)-Human protein phosphatase 1, regulatory (inhibitor) subunit 12A (PPP1R12A), transcript variant 3 |
CNY 10,260.00 |
|
| RC227497L3 | Lenti-ORF clone of PPP1R12A (Myc-DDK-tagged)-Human protein phosphatase 1, regulatory (inhibitor) subunit 12A (PPP1R12A), transcript variant 3 |
CNY 12,160.00 |
|
| RC227497L4 | Lenti-ORF clone of PPP1R12A (mGFP-tagged)-Human protein phosphatase 1, regulatory (inhibitor) subunit 12A (PPP1R12A), transcript variant 3 |
CNY 12,160.00 |
|
| RG227497 | PPP1R12A (tGFP-tagged) - Human protein phosphatase 1, regulatory (inhibitor) subunit 12A (PPP1R12A), transcript variant 3 |
CNY 11,310.00 |
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