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RAD54 (RAD54L) (NM_001142548) Human Untagged Clone
CAT＃: SC325315
RAD54L (untagged)-Human RAD54-like (S. cerevisiae) (RAD54L), transcript variant 2
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | hHR54; HR54; hRAD54; RAD54A |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC325315 representing NM_001142548.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAGGAGGAGCTTGGCTCCCAGCCAGCTGGCCAAGAGAAAACCTGAAGGCAGGTCCTGTGATGATGAA GACTGGCAACCTGGCCTAGTGACTCCTAGGAAACGGAAATCCAGCAGTGAGACCCAGATCCAGGAGTGT TTCCTGTCTCCTTTTCGGAAACCTTTGAGTCAGCTAACCAATCAACCACCTTGTCTGGACAGCAGTCAG CATGAAGCATTTATTCGAAGCATTTTGTCAAAGCCTTTCAAAGTCCCCATTCCAAATTATCAAGGTCCT CTGGGCTCTCGAGCATTGGGCCTGAAAAGGGCTGGGGTCCGCCGGGCCCTCCATGACCCCCTGGAAAAA GATGCCTTGGTTCTGTATGAGCCTCCCCCGCTGAGCGCTCATGACCAGCTGAAGCTTGACAAGGAGAAA CTCCCTGTCCATGTGGTTGTTGACCCTATTCTCAGTAAGGTTTTGCGGCCTCATCAGAGAGAGGGAGTG AAATTCCTGTGGGAGTGTGTCACCAGTCGGCGCATCCCTGGCAGCCATGGCTGCATCATGGCTGATGAG ATGGGCCTAGGAAAGACGCTGCAGTGCATCACATTGATGTGGACACTTTTACGCCAGAGTCCAGAGTGC AAGCCAGAAATTGACAAGGCAGTGGTGGTGTCGCCTTCCAGCCTGGTGAAGAACTGGTACAATGAGGTT GGGAAATGGCTCGGAGGGAGGATCCAACCTCTGGCCATCGATGGAGGATCTAAGGATGAAATAGACCAA AAGCTGGAAGGATTCATGAACCAGCGTGGAGCCAGGGTGTCTTCTCCCATCCTCATCATTTCCTATGAG ACCTTCCGCCTTCATGTTGGAGTCCTCCAGAAAGGAAGTGTTGGTCTGGTCATATGTGACGAGGGACAC AGGCTCAAGAACTCTGAGAATCAGACTTACCAAGCCCTGGACAGCTTGAACACCAGCCGGCGGGTGCTC ATCTCCGGAACTCCCATCCAGAATGATCTGCTTGAGTATTTCAGCTTGGTACATTTTGTTAATTCCGGC ATCCTAGGGACTGCCCATGAATTCAAGAAGCATTTTGAATTGCCAATTTTGAAGGGTCGAGACGCTGCT GCTAGTGAGGCAGACAGGCAGCTAGGAGAGGAGCGGCTGCGGGAGCTCACCAGCATTGTGAATAGATGC CTGATACGGAGGACTTCTGATATCCTTTCTAAATATCTGCCTGTGAAGATTGAGCAGGTCGTTTGTTGT AGGCTGACACCCCTTCAGACTGAGTTATACAAGAGGTTTCTGAGACAAGCCAAACCGGCAGAAGAATTG CTTGAGGGCAAGATGAGTGTGTCTTCCCTTTCTTCCATCACCTCGCTAAAGAAGCTTTGTAATCATCCA GCTCTAATCTATGATAAGTGTGTGGAAGAGGAGGATGGCTTTGTGGGTGCCTTGGACCTCTTCCCTCCT GGTTACAGCTCTAAGGCCCTGGAGCCCCAGCTGTCAGGTAAGATGCTGGTCCTGGATTATATTCTGGCG GTGACCCGAAGCCGTAGCAGTGACAAAGTAGTGCTGGTGTCGAATTACACCCAGACTTTGGATCTCTTT GAGAAGCTGTGCCGTGCCCGAAGGTACTTATACGTCCGCCTGGATGGCACGATGTCCATTAAGAAGCGA GCCAAGGTTGTAGAACGCTTCAATAGTCCATCGAGCCCTGACTTTGTCTTCATGCTGAGCAGCAAAGCT GGGGGCTGTGGCCTCAATCTCATTGGGGCTAACCGGCTGGTCATGTTTGACCCTGACTGGAACCCAGCC AATGATGAACAAGCCATGGCCCGGGTCTGGCGAGATGGTCAAAAGAAGACTTGCTATATCTACCGCCTG CTGTCTGCAGGGACCATTGAGGAGAAGATCTTCCAGCGTCAGAGCCACAAGAAGGCACTGAGCAGCTGT GTGGTGGATGAGGAGCAGGATGTAGAGCGCCACTTCTCTCTGGGCGAGTTGAAGGAGCTGTTTATCCTG GATGAAGCTAGCCTCAGTGACACACATGACAGGTTGCACTGCCGACGTTGTGTCAACAGCCGTCAGATC CGGCCACCCCCTGATGGTTCTGACTGCACTTCAGACCTGGCAGGGTGGAACCACTGCACTGATAAGTGG GGGCTCCGGGATGAGGTACTCCAGGCTGCCTGGGATGCTGCCTCCACTGCCATCACCTTCGTCTTCCAC CAGCGTTCTCATGAGGAGCAGCGGGGCCTCCGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001142548 |
| Insert Size | 2244 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001142548.1 |
| RefSeq Size | 2567 bp |
| RefSeq ORF | 2244 bp |
| Locus ID | 8438 |
| UniProt ID | Q92698 |
| Protein Families | Druggable Genome, Stem cell - Pluripotency |
| Protein Pathways | Homologous recombination |
| MW | 84.4 kDa |
| Gene Summary | The protein encoded by this gene belongs to the DEAD-like helicase superfamily, and shares similarity with Saccharomyces cerevisiae Rad54, a protein known to be involved in the homologous recombination and repair of DNA. This protein has been shown to play a role in homologous recombination related repair of DNA double-strand breaks. The binding of this protein to double-strand DNA induces a DNA topological change, which is thought to facilitate homologous DNA paring, and stimulate DNA recombination. Alternative splicing results in multiple transcript variants encoding the same protein.[provided by RefSeq, Dec 2008] Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same isoform (1). |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC227119 | RAD54L (Myc-DDK-tagged)-Human RAD54-like (S. cerevisiae) (RAD54L), transcript variant 2 |
CNY 5,472.00 |
|
| RC227119L3 | Lenti ORF clone of Human RAD54-like (S. cerevisiae) (RAD54L), transcript variant 2, Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC227119L4 | Lenti ORF clone of Human RAD54-like (S. cerevisiae) (RAD54L), transcript variant 2, mGFP tagged |
CNY 7,700.00 |
|
| RG227119 | RAD54L (tGFP-tagged) - Human RAD54-like (S. cerevisiae) (RAD54L), transcript variant 2 |
CNY 6,370.00 |
