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PARN (NM_001134477) Human Untagged Clone
CAT＃: SC325208
PARN (untagged)-Human poly(A)-specific ribonuclease (deadenylation nuclease) (PARN), transcript variant 2
CNY 7,220.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | DAN; DKCB6; PFBMFT4 |
| Vector | pCMV6 series |
| Sequence Data |
>NCBI ORF sequence for NM_001134477, the custom clone sequence may differ by one or more nucleotides
ATGGACTTTTTGCTATTTCAGTTTGGCCTTTGCACTTTTAAGTATGACTACACAGATTCA AAGTATATAACGAAGTCATTTAACTTCTATGTTTTCCCGAAACCCTTCAATAGATCCTCA CCAGATGTCAAATTTGTTTGTCAGAGCTCCAGCATTGACTTTCTAGCAAGCCAGGGATTT GATTTTAATAAAGTTTTTCGAAATGGAATTCCATATTTAAATCAGGAAGAAGAAAGACAG TTAAGAGAGCAGTATGATGAAAAACGTTCACAGGCGAATGGTGCAGGAGCTCTGTCCTAT GTATCTCCTAACACTTCAAAATGTCCTGTCACGATTCCTGAGGATCAAAAGAAGTTTATT GACCAAGTGGTAGAGAAAATAGAGGATTTATTACAAAGTGAAGAAAACAAGAACTTGGAT TTAGAGCCATGTACCGGGTTCCAAAGAAAACTAATTTATCAGACTTTGAGCTGGAAGTAT CCGAAAGGCATTCATGTTGAGACTTTAGAAACTGAAAAGAAGGAGCGATATATAGTTATC AGCAAAGTAGATGAAGAAGAACGCAAAAGAAGAGAGCAGCAGAAACATGCCAAAGAACAG GAGGAGCTGAATGATGCTGTGGGATTTTCTAGAGTCATTCACGCCATTGCTAATTCGGGA AAACTTGTTATTGGACACAATATGCTCTTGGACGTCATGCACACAGTTCATCAGTTCTAC TGCCCTCTGCCTGCGGACTTAAGTGAGTTTAAAGAGATGACAACATGTGTTTTCCCCAGA CTCTTGGATACTAAATTGATGGCCAGCACACAACCTTTTAAGGATATCATTAACAACACA TCCCTTGCGGAATTGGAAAAGCGGTTAAAAGAGACACCTTTCAACCCTCCTAAAGTTGAA AGTGCCGAAGGTTTTCCAAGTTATGACACAGCCTCTGAACAACTCCACGAGGCAGGCTAC GATGCCTACATCACAGGGCTGTGCTTCATCTCCATGGCCAATTACCTAGGTTCTTTTCTC AGCCCTCCAAAAATTCATGTGTCTGCCAGATCAAAACTCATTGAACCTTTTTTTAACAAG TTATTTCTTATGAGGGTCATGGATATCCCCTATCTAAACTTGGAAGGACCAGACTTGCAG CCTAAACGTGATCATGTTCTCCATGTGACATTCCCCAAAGAATGGAAAACCAGCGACCTT TACCAGCTTTTCAGTGCCTTTGGTAACATTCAGATATCCTGGATTGATGACACATCAGCA TTTGTTTCCCTTAGCCAGCCCGAGCAAGTAAAGATTGCTGTCAATACCAGCAAATATGCA GAAAGCTATCGGATCCAAACCTATGCTGAATATATGGGGAGAAAACAGGAAGAGAAGCAG ATCAAAAGAAAGTGGACTGAAGATAGCTGGAAGGAGGCTGACAGCAAACGGTTAAACCCC CAGTGCATACCCTACACCCTGCAGAATCACTATTACCGCAACAATAGTTTTACAGCTCCC AGCACAGTAGGAAAGAGAAATTTGAGTCCTAGTCAAGAGGAAGCTGGCCTGGAGGACGGA GTGTCAGGGGAGATTTCCGACACTGAGCTTGAGCAGACCGATTCCTGTGCAGAGCCCCTC TCAGAGGGAAGGAAAAAGGCCAAGAAATTAAAAAGAATGAAGAAGGAGCTTTCTCCAGCA GGAAGCATCTCGAAGAACAGCCCTGCCACACTCTTTGAAGTTCCTGACACATGG |
| Restriction Sites | Please inquire |
| ACCN | NM_001134477 |
| Insert Size | 3000 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001134477.1, NP_001127949.1 |
| RefSeq Size | 3000 bp |
| RefSeq ORF | 3000 bp |
| Locus ID | 5073 |
| UniProt ID | O95453 |
| Protein Families | Transcription Factors |
| Protein Pathways | RNA degradation |
| Gene Summary | The protein encoded by this gene is a 3'-exoribonuclease, with similarity to the RNase D family of 3'-exonucleases. It prefers poly(A) as the substrate, hence, efficiently degrades poly(A) tails of mRNAs. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs. This protein is also involved in silencing of certain maternal mRNAs during oocyte maturation and early embryonic development, as well as in nonsense-mediated decay (NMD) of mRNAs that contain premature stop codons. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008] Transcript Variant: This variant (2) uses an alternate donor splice site in the 5' terminal exon compared to transcript variant 1, resulting in translation initiation from an alternate, in-frame downstream AUG and a shorter isoform (2) compared to isoform 1. |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Human Telomerase RNA Processing and Quality Control
,Tseng, CK;Wang, HF;Burns, AM;Schroeder, MR;Gaspari, M;Baumann, P;,
Cell Rep
,PubMed ID 26628367
[PARN]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC225978 | PARN (Myc-DDK-tagged)-Human poly(A)-specific ribonuclease (deadenylation nuclease) (PARN), transcript variant 2 |
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| RC225978L3 | Lenti-ORF clone of PARN (Myc-DDK-tagged)-Human poly(A)-specific ribonuclease (deadenylation nuclease) (PARN), transcript variant 2 |
CNY 8,270.00 |
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| RC225978L4 | Lenti-ORF clone of PARN (mGFP-tagged)-Human poly(A)-specific ribonuclease (deadenylation nuclease) (PARN), transcript variant 2 |
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| RG225978 | PARN (tGFP-tagged) - Human poly(A)-specific ribonuclease (deadenylation nuclease) (PARN), transcript variant 2 |
CNY 7,030.00 |
