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EDC3 (NM_001142443) Human Untagged Clone
CAT＃: SC325143
EDC3 (untagged)-Human enhancer of mRNA decapping 3 homolog (S. cerevisiae) (EDC3), transcript variant 1
CNY 7,220.00
Product images
CNY 6,281.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | hYjeF_N2-15q23; LSM16; MRT50; YJDC; YJEFN2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC325143 representing NM_001142443.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCTACAGATTGGCTGGGAAGTATTGTGTCCATCAATTGTGGAGATAGCTTGGGTGTCTATCAGGGA AGAGTGTCAGCTGTGGATCAGGTCAGCCAGACCATTTCTCTCACCCGGCCTTTCCATAATGGAGTGAAG TGTCTTGTTCCAGAAGTCACCTTCAGGGCAGGTGACATTACGGAGTTAAAAATTCTGGAGATACCAGGA CCTGGAGACAACCAACATTTTGGAGACCTTCATCAAACAGAATTAGGCCCCTCTGGTGCTGGCTGCCAA GTGGGCATCAATCAGAATGGCACAGGCAAGTTTGTCAAGAAGCCAGCCTCTTCCAGCAGTGCCCCTCAG AATATCCCTAAGAGGACAGATGTGAAGAGCCAGGATGTTGCCGTTTCCCCGCAGCAGCAACAGTGCTCA AAGAGCTATGTCGACAGGCACATGGAATCCTTGAGTCAGTCCAAAAGTTTCCGTCGTCGGCACAACTCC TGGTCATCTAGTAGCAGGCACCCAAATCAGGCAACTCCCAAGAAAAGTGGTTTAAAGAATGGCCAGATG AAGAATAAAGATGACGAGTGCTTCGGGGATGATATTGAGGAGATCCCAGACACAGATTTTGATTTTGAA GGGAACCTGGCTCTTTTTGACAAGGCAGCTGTGTTTGAGGAGATTGATACCTATGAAAGGAGAAGTGGT ACCCGTTCCCGGGGCATCCCAAATGAAAGGCCCACTCGGTACCGCCATGATGAGAACATCTTGGAGTCC GAGCCCATTGTCTATCGACGGATCATAGTGCCCCACAACGTGAGCAAGGAGTTCTGCACGGACTCTGGC CTGGTTGTCCCAAGTATTTCCTATGAGCTGCATAAAAAGCTGTTGTCCGTGGCTGAGAAGCATGGGCTG ACCCTTGAGCGGAGACTGGAGATGACAGGTGTGTGTGCCAGTCAGATGGCACTGACCCTCCTCGGAGGA CCTAACAGGTTGAATCCCAAAAATGTTCACCAGAGGCCTACAGTGGCTCTACTGTGTGGACCTCATGTG AAGGGGGCTCAGGGTATCAGCTGTGGAAGGCACCTAGCCAACCATGATGTCCAGGTCATCCTTTTCCTG CCCAATTTTGTCAAGATGTTGGAATCTATCACCAATGAGCTGTCGCTCTTCAGCAAGACCCAAGGCCAA CAAGTGTCTAGCCTCAAAGATCTGCCCACTAGCCCTGTGGACCTGGTCATCAACTGCCTGGATTGCCCT GAGAACGTCTTCCTGCGCGATCAACCCTGGTACAAGGCAGCTGTGGCCTGGGCCAACCAGAACCGGGCA CCAGTACTCAGCATAGACCCTCCTGTGCATGAAGTCGAACAGGGCATTGATGCCAAATGGTCACTGGCA CTGGGCCTGCCTCTGCCACTGGGGGAGCACGCAGGCCGTATCTATTTGTGCGACATTGGCATTCCCCAG CAGGTCTTCCAGGAGGTGGGCATCAACTACCACTCGCCCTTTGGCTGCAAGTTTGTTATCCCACTGCAC TCTGCTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001142443 |
| Insert Size | 1527 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001142443.1 |
| RefSeq Size | 4086 bp |
| RefSeq ORF | 1527 bp |
| Locus ID | 80153 |
| UniProt ID | Q96F86 |
| Protein Pathways | RNA degradation |
| MW | 56.1 kDa |
| Gene Summary | This gene encodes a protein that is important in mRNA degradation. The encoded protein is a component of a decapping complex that promotes efficient removal of the monomethylguanosine (m7G) cap from mRNAs, as part of the 5' to 3' mRNA decay pathway. Mutations in this gene have been identified in human patients with an autosomal recessive form of intellectual disability. [provided by RefSeq, May 2017] Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Variants 1-4 encode the same isoform (1). |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC226857 | EDC3 (Myc-DDK-tagged)-Human enhancer of mRNA decapping 3 homolog (S. cerevisiae) (EDC3), transcript variant 1 |
CNY 3,784.00 |
|
| RC226857L3 | Lenti ORF clone of Human enhancer of mRNA decapping 3 homolog (S. cerevisiae) (EDC3), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC226857L4 | Lenti ORF clone of Human enhancer of mRNA decapping 3 homolog (S. cerevisiae) (EDC3), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG226857 | EDC3 (tGFP-tagged) - Human enhancer of mRNA decapping 3 homolog (S. cerevisiae) (EDC3), transcript variant 1 |
CNY 4,370.00 |
