IST1 (NM_014761) Human Untagged Clone

CAT#: SC324384

IST1 (untagged)-Human KIAA0174 (KIAA0174)



  "NM_014761" in other vectors (7)

CNY 3,656.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


IST1 rabbit polyclonal antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms CHMP8; OLC1
Vector pCMV6-AC
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>OriGene sequence for NM_014761.2 GAACCCTGAAGTCGGTGTCTGCTGCGTTCACGGCAGGATTCGGTTAGGAGGAACAGCACA
GCATGCTGGGCTCTGGATTTAAAGCTGAGCGCTTAAGAGTGAATTTGAGATTAGTCATAA
ATCGCCTTAAACTATTGGAGAAAAAGAAAACGGAACTGGCCCAGAAAGCAAGGAAGGAGA
TTGCTGACTATCTGGCTGCTGGGAAAGATGAACGAGCTCGGATCCGTGTGGAGCACATTA
TCCGGGAAGACTACCTCGTGGAGGCCATGGAGATCCTGGAGCTGTACTGTGACCTGCTGC
TGGCTCGGTTTGGCCTTATCCAGTCTATGAAGGAACTAGATTCTGGTCTGGCTGAATCTG
TGTCTACATTGATCTGGGCTGCTCCTCGACTCCAGTCAGAAGTGGCTGAGTTGAAAATAG
TTGCTGATCAGCTCTGTGCCAAGTATAGCAAGGAATATGGCAAGCTATGTAGGACCAACC
AGATTGGAACTGTGAATGACAGGCTAATGCACAAGCTGAGTGTGGAAGCCCCACCCAAAA
TCCTGGTGGAGAGATACCTGATTGAAATTGCAAAGAATTACAACGTACCCTATGAACCTG
ACTCTGTGGTCATGGCAGAAGCTCCTCCTGGGGTAGAGACAGATCTTATTGATGTTGGAT
TCACAGATGATGTGAAGAAAGGAGGCCCTGGAAGAGGAGGGAGTGGTGGCTTCACAGCAC
CAGTTGGTGGACCTGATGGAACGGTGCCAATGCCCATGCCCATGCCCATGCCTATGCCAT
CTGCAAATACGCCTTTCTCATATCCACTGCCAAAGGGACCATCAGATTTCAATGGACTGC
CAATGGGGACTTATCAGGCCTTTCCCAATATTCATCCACCTCAGATACCAGCAACTCCCC
CATCGTATGAATCTATGACATTAATGCTGATAAGAATATCTCTTCTGCACAGATTGTTGG
TCCTGGACCCAAGCCAGAAGCCTCTGCAAAGCTTCCTTCCAGACCTGCAGATAACTATGA
CAACTTTGTCCTACCAGAGTTGCCATCTGTGCCAGACACACTACCAACTGCATCTGCTGG
TGCCAGCACCTCAGCATCTGAAGACATTGACTTTGATGATCTTTCCCGGAGGTTTGAAGA
GCTGAAAAAGAAAACATAGGTCTCTTAAACCAGGCAACTTTCACGTTTTGGGAGTTGAGA
CTGAGCAATTTCTCCTTGTAACAAAGAATCTCCATGAAATTCTGTTTCATCTGTTAACCG
TCACTCAGCACAACACTCCCTCTGGGCTCTCTTCCTGCTCCTCCAGATTCTGCTGCTTTC
CAGTTCTCTGTTGATCCTGAGACTAACAATTGGAGACTGAGGCCAGAGCAACTGGCTCCT
GGCAGCTGTGCTTGTCCGTTTCCTGTCAGAGTGATCCCAGGTTTCCTCCTGGCCCGTCCC
ATGGTCCCTCCACAGGAGTGTGAGAGGATGGGGGAAGCACTGTGGGAAGACCACCAAAGA
TGGCTGGACAGTGGGAGAGAGCACGTTGTGAAGCATCCCAGCCTCGTGTTGAGGTTCCAG
ACTTAGAAACAGACCCCTCTGTACAGGGGGATTGTGGTGAGTGAGAATCAAGGCCACCTT
GTGTGTTTTCTCACTCTCGAATGCAAGTGGGAGAGGGAAAATGACTCGGGACGCCATTGT
AACGGTTCCTGGAAGCTGGGCCCTCTCATTGGCATATACAGTACTCCTCGCTGCAGGGCA
CTGTCCCACCGGGATCCAGTTGCAAAGTTTGTCTTGACAGTTGAAGGCCTCGCTTAGTTG
TACTGGATTCTCAGGGAGCCCTCTGTGGCCTTTTGCTTTGCGTGCTGTTTCCCTTGTACC
AGAGGGCGGCACCGTGGAAATTCTGTTTTCCCTGTAGCATATTGTGTTGGATTGCATTAC
TGGCAGAGAAAGGACAAGGTGCCATTCAAGTCCTAGGGTGGGCTTCCAGCTGCCTTAATA
GAAGTACTCAAGTCTTTTGGGTAGTGAGCTGGAAAGCCTACAGGAAAAGAGGGGTACCTG
TTTTCATTTGAAAACTTTGATTCATGGAACCTTTAAAACTAATCTCAGAAAAATTTTTGG
TGCCCATGCAGCTGTAGTTGTTCACTGCTTTCCTGGATGGATGGGACTCTTATGTCATAA
CTTCTGTTACTCCTTTGGCCCATAGCTAAGGTCATCCTTCCCCACAGGGGTGGCTTTGGG
ATTGGATGATACAGCTTTTGCTTCTGTGTAGTATACCTGTACATACTTGTTTCAGGCAGC
CTTTCTTTAATGTTTTCAGTTGGTTTGTATTCTGTAGCTCAGTAGCTGCTAATAAAGTTA
AAGATCCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites Please inquire     
ACCN NM_014761
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_014761.2, NP_055576.2
RefSeq Size 2377 bp
RefSeq ORF 1083 bp
Locus ID 9798
UniProt ID P53990
Domains DUF292
Gene Summary This gene encodes a protein with MIT-interacting motifs that interacts with components of endosomal sorting complexes required for transport (ESCRT). ESCRT functions in vesicle budding, such as that which occurs during membrane abscission in cytokinesis. There is a pseudogene for this gene on chromosome 19. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2012]
Transcript Variant: This variant (2) uses an alternate splice site in the coding region, which results in a frameshift, compared to variant 1. The encoded isoform (b, also known as hIST1b) has a distinct C-terminus and is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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