United States
Germany
Japan
United Kingdom
China
BCAR3 (NM_003567) Human Untagged Clone
CAT＃: SC324095
BCAR3 (untagged)-Human breast cancer anti-estrogen resistance 3 (BCAR3)
CNY 6,048.00
| Cited in 2 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | AND-34; MIG7; NSP2; SH2D3B |
| Vector | pCMV6-AC |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>OriGene sequence for NM_003567.2
CCACGCGTCCGGCGTCCCCAGCTCCGGGAGCAGCCGCGGCGCGGGCGGTGCGAGGCCGGC
CGCGCGGCGCCTTTGAGCCCCAGCCGGAGGTCCAGAGATCCTGAGAGCCCGAGCGCCCAG CGCCGCGCCGGGAGAGTTGAGAATTATGGCTGCAGGAAAATTTGCAAGCCTTCCCAGAAA CATGCCGGTGAATCACCAGTTCCCCCTGGCCTCATCCATGGACCTTCTGAGCAGCAGGTC CCCTCTCGCTGAGCATCGCCCAGATGCCTATCAAGATGTGTCTATACATGGCACCCTTCC ACGGAAGAAAAAAGGTCCTCCTCCCATAAGGTCCTGTGATGACTTCAGTCACATGGGCAC CCTCCCCCACTCCAAATCCCCACGGCAGAACTCGCCTGTGACCCAGGATGGCATCCAGGA GAGCCCATGGCAGGACCGGCACGGCGAAACCTTCACCTTCAGGGATCCACATCTTCTGGA CCCAACTGTGGAATATGTGAAGTTCTCCAAGGAGAGGCACATCATGGACAGGACCCCCGA GAAACTGAAGAAGGAGCTGGAGGAGGAGCTGCTCCTGAGCAGCGAGGACCTGCGCAGCCA TGCCTGGTACCACGGCCGCATCCCCCGACAGGTGTCTGAAAACCTTGTGCAGCGAGATGG TGACTTCCTAGTTCGTGACTCTCTGTCCAGCCCTGGGAACTTTGTCCTGACCTGTCAGTG GAAGAACCTCGCTCAGCACTTCAAAATCAACCGGACAGTTCTGCGACTCAGCGAGGCCTA CAGCCGCGTGCAGTACCAGTTCGAGATGGAGAGCTTCGACTCCATCCCCGGCCTGGTGCG CTGCTACGTGGGCAACCGCCGGCCCATCTCCCAGCAGAGTGGCGCCATCATCTTCCAGCC CATCAACAGGACGGTGCCTCTGCGGTGCCTGGAGGAGCATTATGGCACCTCCCCAGGCCA GGCCCGGGAGGGCAGCCTCACCAAGGGAAGGCCGGATGTGGCCAAGAGGCTGAGCCTCAC CATGGGTGGCGTCCAGGCCCGAGAGCAGAATTTGCCCAGGGGAAACCTCCTCAGAAACAA AGAAAAGAGTGGTAGCCAGCCCGCCTGCCTGGATCACATGCAGGACAGAAGAGCCTTGTC CCTCAAAGCCCACCAGTCAGAGAGCTACCTGCCGATTGGCTGCAAGCTGCCACCTCAGTC CTCGGGTGTGGACACAAGCCCCTGCCCAAACTCACCTGTGTTCAGGACGGGAAGCGAGCC TGCCCTGAGCCCAGCAGTGGTTCGGAGGGTCTCCTCAGACGCCAGGGCTGGGGAGGCGCT GAGGGGATCAGACAGTCAACTGTGCCCTAAGCCCCCGCCTAAGCCCTGCAAGGTGCCGTT CCTCAAGGTTCCCTCGTCTCCCTCTGCCTGGCTCAACTCAGAGGCCAACTACTGTGAACT GAACCCAGCGTTTGCCACAGGCTGCGGCAGGGGAGCAAAGCTACCCTCATGTGCCCAGGG AAGCCACACAGAACTGCTCACAGCCAAGCAGAATGAGGCGCCAGGTCCCCGGAACTCTGG CGTCAACTACTTGATCCTTGATGATGATGACAGGGAAAGACCTTGGGAACCTGCGGCAGC TCAGATGGAGAAGGGGCAGTGGGACAAGGGCGAGTTTGTGACGCCCCTCCTGGAGACTGT CTCCTCCTTCAGGCCCAACGAGTTTGAGTCAAAGTTCCTTCCCCCTGAGAATAAGCCCCT GGAAACAGCAATGTTGAAACGTGCAAAAGAACTGTTCACCAACAACGACCCCAAGGTCAT CGCCCAGCACGTACTGAGCATGGACTGCAGGGTTGCTAGGATACTTGGAGTCTCTGAAGA GATGAGGAGGAACATGGGGGTGAGCTCAGGCCTGGAACTCATTACCTTGCCTCACGGACA CCAGCTGCGCCTGGACATAATTGAAAGACACAACACAATGGCCATCGGCATTGCAGTGGA CATTCTGGGATGCACGGGCACTTTGGAGGACCGAGCGGCCACTCTGAGTAAGATCATCCA GGTGGCGGTGGAACTGAAGGATTCCATGGGGGACCTCTATTCCTTCTCAGCTCTCATGAA AGCCCTGGAAATGCCACAGATCACAAGGTTAGAAAAGACGTGGACTGCTCTGCGGCACCA GTACACCCAAACTGCCATTCTCTATGAGAAACAGCTGAAGCCCTTCAGCAAACTCCTGCA TGAAGGCAGAGAGTCCACATGTGTTCCCCCAAACAATGTATCAGTCCCACTGCTGATGCC GCTTGTGACGTTAATGGAGCGCCAGGCTGTGACTTTTGAAGGAACCGACATGTGGGAAAA AAACGACCAGAGCTGTGAAATCATGCTGAACCATTTGGCAACAGCGCGATTCATGGCCGA GGCTGCAGACAGCTACCGGATGAATGCTGAGAGGATCCTGGCAGGTTTTCAACCAGATGA AGAAATGAATGAAATCTGCAAGACTGAATTTCAAATGCGATTGCTATGGGGCAGCAAAGG TGCACAAGTCAATCAGACAGAGAGATATGAGAAATTCAACCAGATTTTAACTGCCCTCTC GCGTAAATTGGAACCTCCTCCTGTAAAGCAGGCAGAGCTTTGATAACTCTCCAGAGAACC TTTAGAATATCTTTTCAAGTTTCCCCAGCTTCATCTTTGGGAAAGCTTACTGTTTTTGAT AAAGTAATAATGTGCAAATCTGACAATATACAAGCTTTTAGTATCCACAGGATATTAAAC GTGTAAATTGCACAGAGCACACTTATTTATGAATTGTCTAAAGTTACTACTGATTTTAAA ATGAATAATTTATTATTAAGGTAACTACTGCTAATGTTGATCAGCAAATTTAAGAGAAGA CCTAGCTATGTTGGCTGGTTGCTTTCTATTATCATGGTATTTGACCATTTTAGTTTTAAT TCCATGTCAGATAAGTGTAAATAGAAGAGTTTAAAAGCATGAAACATTTCAGAAGGTATC AGTTATATGATATTCTTTAAACAAATATGAAAAATGTAAATACTCATGAATGAAAATACA TCTTTTTGTGAAAAAAAAAAAAAAAAAAA |
| Restriction Sites | ECoRI-NOT |
| ACCN | NM_003567 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003567.2, NP_003558.1 |
| RefSeq Size | 3184 bp |
| RefSeq ORF | 2478 bp |
| Locus ID | 8412 |
| UniProt ID | O75815 |
| Domains | SH2, RasGEF |
| Protein Families | Druggable Genome |
| Gene Summary | Breast tumors are initially dependent on estrogens for growth and progression and can be inhibited by anti-estrogens such as tamoxifen. However, breast cancers progress to become anti-estrogen resistant. Breast cancer anti-estrogen resistance gene 3 was identified in the search for genes involved in the development of estrogen resistance. The gene encodes a component of intracellular signal transduction that causes estrogen-independent proliferation in human breast cancer cells. The protein contains a putative src homology 2 (SH2) domain, a hall mark of cellular tyrosine kinase signaling molecules, and is partly homologous to the cell division cycle protein CDC48. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012] Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein (isoform 1). |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Protein Tyrosine Phosphatase a Phosphotyrosyl-789 Binds BCAR3 To Position Cas for Activation at Integrin-Mediated Focal Adhesions
,Guobin Sun, Suzanne Y. S. Cheng, Min Chen, Chinten James Lim, and Catherine J. Pallen,
Mol. Cell. Biol., Sep 2012; 32: 3776 - 3789.
[BCAR3]
|
|
Breast cancer antiestrogen resistance-3 expression regulates breast cancer cell migration through promotion of p130Cas membrane localization and membrane ruffling
,RS Schrecengost, RB Riggins, KS Thomas, MS Guerrero, and AH Bouton,
Cancer Res. 2007 Jul 1;67(13):6174-82.
[BCAR3]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207930 | BCAR3 (Myc-DDK-tagged)-Human breast cancer anti-estrogen resistance 3 (BCAR3) |
CNY 6,040.00 |
|
| RC207930L1 | Lenti ORF clone of Human breast cancer anti-estrogen resistance 3 (BCAR3), Myc-DDK-tagged |
CNY 8,440.00 |
|
| RC207930L2 | Lenti ORF clone of Human breast cancer anti-estrogen resistance 3 (BCAR3), mGFP tagged |
CNY 7,700.00 |
|
| RC207930L3 | Lenti ORF clone of Human breast cancer anti-estrogen resistance 3 (BCAR3), Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC207930L4 | Lenti ORF clone of Human breast cancer anti-estrogen resistance 3 (BCAR3), mGFP tagged |
CNY 7,700.00 |
|
| RG207930 | BCAR3 (tGFP-tagged) - Human breast cancer anti-estrogen resistance 3 (BCAR3) |
CNY 7,640.00 |
|
| SC125362 | BCAR3 (untagged)-Human breast cancer anti-estrogen resistance 3 (BCAR3) |
CNY 2,950.00 |
