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UPF1 (NM_002911) Human Untagged Clone
CAT＃: SC323999
UPF1 (untagged)-Human UPF1 regulator of nonsense transcripts homolog (yeast) (UPF1)
CNY 7,792.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | HUPF1; NORF1; pNORF1; RENT1; smg-2; UTF |
| Vector | pCMV6-AC |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>OriGene sequence for NM_002911.3
GGCGACGGCGGCGGTGGCGGCAGTTCCTGCTCTAGGCTGCGAGCGGCTGGCGGCTTCGAG
GGGAGCTGAGGCGCGGAGGGGCTCGGCGGCAGCGGCGGCGGCTCGGCACTGTTACCTCTC GGTCCGGCTGGCGCCGGGGCGGGCGGTTTGGTCCTTTCCGGGCGCGCGGGGGCGACAGCG GCAGCGACCCGAGGCCTGCGGCCTAGGCCTCAGCGCGGCGGCGGGCTCGAGTGCAGCGCG GAACCGGCCCGAGGGCCCTACCCGGAGGCACCATGAGCGTGGAGGCGTACGGGCCCAGCT CGCAGACTCTCACTTTCCTGGACACGGAGGAGGCCGAGCTGCTTGGCGCCGACACACAGG GCTCCGAGTTCGAGTTCACCGACTTTACTCTTCCTAGCCAGACGCAGACGCCCCCCGGCG GCCCCGGCGGCCCGGGCGGTGGCGGCGCGGGAGGCCCGGGCGGCGCGGGCGCGGGCGCTG CGGCGGGACAGCTCGACGCGCAGGTTGGGCCCGAAGGCATCCTGCAGAACGGGGCTGTGG ACGACAGTGTAGCCAAGACCAGCCAGTTGTTGGCTGAGTTGAACTTCGAGGAAGATGAAG AAGACACCTATTACACGAAGGACCTCCCCATACACGCCTGCAGTTACTGTGGAATACACG ATCCTGCCTGCGTGGTTTACTGTAATACCAGCAAGAAGTGGTTCTGCAACGGACGTGGAA ATACTTCTGGCAGCCACATTGTAAATCACCTTGTGAGGGCAAAATGCAAAGAGGTGACCC TGCACAAGGACGGGCCCCTGGGGGAGACAGTCCTGGAGTGCTACAACTGCGGCTGTCGCA ACGTCTTCCTCCTCGGCTTCATCCCGGCCAAAGCTGACTCAGTGGTGGTGCTGCTGTGCA GGCAGCCCTGTGCCAGCCAGAGCAGCCTCAAGGACATCAACTGGGACAGCTCGCAGTGGC AGCCGCTGATCCAGGACCGCTGCTTCCTGTCCTGGCTGGTCAAGATCCCCTCCGAGCAGG AGCAGCTGCGGGCACGCCAGATCACGGCACAGCAGATCAACAAGCTGGAGGAGCTGTGGA AGGAAAACCCTTCTGCCACGCTGGAGGACCTGGAGAAGCCGGGGGTGGACGAGGAGCCGC AGCATGTCCTCCTGCGGTACGAGGACGCCTACCAGTACCAGAACATATTCGGGCCCCTGG TCAAGCTGGAGGCCGACTACGACAAGAAGCTGAAGGAGTCCCAGACTCAAGATAACATCA CTGTCAGGTGGGACCTGGGCCTTAACAAGAAGAGAATCGCCTACTTCACTTTGCCCAAGA CTGACTCTGACATGCGGCTCATGCAGGGGGATGAGATATGCCTGCGGTACAAAGGGGACC TTGCGCCCCTGTGGAAAGGGATCGGCCACGTCATCAAGGTCCCTGATAATTATGGCGATG AGATCGCCATTGAGCTGCGGAGCAGCGTGGGTGCACCTGTGGAGGTGACTCACAACTTCC AGGTGGATTTTGTGTGGAAGTCGACCTCCTTTGACAGGATGCAGAGCGCATTGAAAACGT TTGCCGTGGATGAGACCTCGGTGTCTGGCTACATCTACCACAAGCTGTTGGGCCACGAGG TGGAGGACGTAATCATCAAGTGCCAGCTGCCCAAGCGCTTCACGGCGCAGGGCCTCCCCG ACCTCAACCACTCCCAGGTTTATGCCGTGAAGACTGTGCTGCAAAGACCACTGAGCCTGA TCCAGGGCCCGCCAGGCACGGGGAAGACGGTGACGTCGGCCACCATCGTCTACCACCTGG CCCGGCAAGGCAACGGGCCGGTGCTGGTGTGTGCTCCGAGCAACATCGCCGTGGACCAGC TAACGGAGAAGATCCACCAGACGGGGCTAAAGGTCGTGCGCCTCTGCGCCAAGAGCCGTG AGGCCATCGACTCCCCGGTGTCTTTTCTGGCCCTGCACAACCAGATCAGGAACATGGACA GCATGCCTGAGCTGCAGAAGCTGCAGCAGCTGAAAGACGAGACTGGGGAGCTGTCGTCTG CCGACGAGAAGCGGTACCGGGCCTTGAAGCGCACCGCAGAGAGAGAGCTGCTGATGAACG CAGATGTCATCTGCTGCACATGTGTGGGCGCCGGTGACCCGAGGCTGGCCAAGATGCAGT TCCGCTCCATTTTAATCGACGAAAGCACCCAGGCCACCGAGCCGGAGTGCATGGTTCCCG TGGTCCTCGGGGCCAAGCAGCTGATCCTTGTAGGCGACCACTGCCAGCTGGGCCCAGTGG TGATGTGCAAGAAGGCGGCCAAGGCCGGGCTGTCACAGTCGCTCTTCGAGCGCCTGGTGG TGCTGGGCATCCGGCCCATCCGCCTGCAGGTCCAGTACCGGATGCACCCTGCACTCAGCG CCTTCCCATCCAACATCTTCTACGAGGGCTCCCTCCAGAATGGTGTCACTGCAGCGGATC GTGTGAAGAAGGGATTTGACTTCCAGTGGCCCCAACCCGATAAACCGATGTTCTTCTACG TGACCCAGGGCCAAGAGGAGATTGCCAGCTCGGGCACCTCCTACCTGAACAGGACCGAGG CTGCGAACGTGGAGAAGATCACCACGAAGTTGCTGAAGGCAGGCGCCAAGCCGGACCAGA TTGGCATCATCACGCCCTACGAGGGCCAGCGCTCCTACCTGGTGCAGTACATGCAGTTCA GCGGCTCCCTGCACACCAAGCTCTACCAGGAGGTGGAGATCGCCAGTGTGGACGCCTTTC AGGGACGCGAGAAGGACTTCATCATCCTGTCCTGTGTGCGGGCCAACGAGCACCAAGGCA TTGGCTTTTTAAATGACCCCAGGCGTCTGAACGTGGCCCTGACCAGAGCAAGGTATGGCG TCATCATTGTGGGCAACCCGAAGGCACTATCAAAGCAGCCGCTCTGGAACCACCTGCTGA ACTACTATAAGGAGCAGAAGGTGCTGGTGGAGGGGCCGCTCAACAACCTGCGTGAGAGCC TCATGCAGTTCAGCAAGCCACGGAAGCTGGTCAACACTATCAACCCGGGAGCCCGCTTCA TGACCACAGCCATGTATGATGCCCGGGAGGCCATCATCCCAGGCTCCGTCTATGATCGGA GCAGCCAGGGCCGGCCTTCCAGCATGTACTTCCAGACCCATGACCAGATTGGCATGATCA GTGCCGGCCCTAGCCACGTGGCTGCCATGAACATTCCCATCCCCTTCAACCTGGTCATGC CACCCATGCCACCGCCTGGCTATTTTGGACAAGCCAACGGGCCTGCTGCAGGGCGAGGCA CCCCGAAAGGCAAGACTGGTCGTGGGGGACGCCAGAAGAACCGCTTTGGGCTTCCTGGAC CCAGCCAGACTAACCTCCCCAACAGCCAAGCCAGCCAGGATGTGGCGTCACAGCCCTTCT CTCAGGGCGCCCTGACGCAGGGCTACATCTCCATGAGCCAGCCTTCCCAGATGAGCCAGC CCGGCCTCTCCCAGCCGGAGCTGTCCCAGGACAGTTACCTTGGTGACGAGTTTAAATCAC AAATCGACGTGGCGCTCTCACAGGACTCCACGTACCAGGGAGAGCGGGCTTACCAGCATG GCGGGGTGACGGGGCTGTCCCAGTATTAAAAGGTGGCGGCGGAAGAGCTAAGCAACGTGG CTTAGTCCATCAGCATCTTATTCTGGGTAATAAAAAATAAAAATAAACGGATACCTGTTT TCCACTGCTAAAACTGAAGCACCACTGTGTGAGCAACAGGAAGGGAGAGCGCACGAGGGA GAGGAGCCGAGGCCGAGCGCCCCCTGCTGGCCCGCGGCGGCGAGGAGCAGAGGGAGCGGA GGAGGGGCCGGCCCGCGGGAGCCGCGGCCACCAGGAGGCCCCGCTCCGTCCCATCGGGGC TGCGGCCAGGGCGGAGGGAGGAAGACCCTCATCTCAGAGTAGCCCTTTCCTCTGTTCTTT TATTTCTTTTTCTCTTTGATTGAAAGGGGACTACGTCTTAGCAGGAAAAAAAACTTCGCA TTTCTGTGCCCGAGCAGGCTCCTTGCAAAGACAGCAGCGTGCGGGGCAGAGCCCCGGGAG GGCGCGTCTGTCCACGCCTACCGGACGCGCCGAGGTCGCGCTGCCTGTGTTCTCCGAGGG CCTTCATTTAAAGAAAATAAGGGTGTTTTGGGTTTTTCTCTTTGTTTTTTTCAAGATTCT TTTAAAGGAGTACTGAAGAATACTTTCCTAAGTTTGTCTCTAAAATCTTAGCGGTGGACC TGGGAGATTTGAGAAGCTTCCAGAAACAGTTTAAACAAGCCAGCGCTACTGGAGAAGAGG AGCAACACCTGTGCCGCGGCCGGAGGAGTTTTGTTGTTGGTTTTAGCTTCCAGTGGCTTC TTTCTGCGGGGCATCAGGCTGCTGGGGTAGCCGCCCGCCGAGCCTGGAAGCTGCTCGTTC TCCGCTGGACTCAGAAGCCAAGCTGCTTCCCGCCTAGACTCGGCGCAGGGCCCCGCACCG GTGAGGAAGGTGCTTTTGGCCCCATTGCGAGGGGCCTTGGCCAGGACTGGCCCTGTGGCC AGGAGGCGAGAAGGTGGCTGTTCCCGGATTGACGGCTTTTTCCCGGGGGCCTTTGGAAGA TTTGGTGGAAGGACAAGAGGGCCTGTCCCTGTCCCCGTCCCCAGGAGGTACCGACAGTCC CTGTGCTGGTTAGACACGGAGCGCTGCACACCGAAAGCCCAAATTGGGAGCTCTGCCTGC CGGCAACTTTGCTGATGGGGTGATTGCTGCTTCTGGGGGGTAAGGAAACAAGTTACAGAA ATTACCGCGTTCTGTGTGAAGGGACTGAGGGTGTGGTGTCATTGGCAGAGGGTCATTTTA GGAGAGCTGCCCCAGCCCCTCGAACGCCTGGCTTGGGGTGTCATTCTGCCTGGCGGCCAG GCCTCCAGCTTCCCCTGCCCCGGGCCTGGGGCTGTCACTGGCCCTGATCCGAACACCTCC AGATTCCGGCTTCTACATGGGACAGACGGGGACGCACAGGCCACCTTCCTTCTGGCAGGG ACTCTTATTTATTCCCATTGCTCTAGGGCTTTCGGTTTCCCCTTCTTCCGGTAGGCCGCG TAGAGGCATGCACCGGGTAGGTTTCCGCGGTGACCCCGCGGCGGCCTGAGGGACGCTCCC TGCCCCATCCCGGCTGTTGGGCTGGGCCGCTTTGCCTCTGCTTCGCCCTGTGCTGTGTTC TCCAGCTTTGTAGCAGCAGCCTTGACAAACCCAGGCGCACTGTACCAAGGCAATGTAACT TTTGATTTTCGGTCAATTTAAGTTCTTTTGTCACCAAATATTAATAAACAGTTTTGACTT CAAAAAAAAAAAAAAAA |
| Restriction Sites | ECoRI-NOT |
| ACCN | NM_002911 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002911.3, NP_002902.2 |
| RefSeq Size | 5360 bp |
| RefSeq ORF | 3357 bp |
| Locus ID | 5976 |
| UniProt ID | Q92900 |
| Protein Families | Druggable Genome |
| Gene Summary | This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located only in the cytoplasm. When translation ends, it interacts with the protein that is a functional homolog of yeast Upf2p to trigger mRNA decapping. Use of multiple polyadenylation sites has been noted for this gene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014] Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. It encodes isoform 2, which is shorter than isoform 1. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208018 | UPF1 (Myc-DDK-tagged)-Human UPF1 regulator of nonsense transcripts homolog (yeast) (UPF1) |
CNY 11,672.00 |
|
| RC208018L1 | Lenti ORF clone of Human UPF1 regulator of nonsense transcripts homolog (yeast) (UPF1), Myc-DDK-tagged |
CNY 14,072.00 |
|
| RC208018L2 | Lenti ORF clone of Human UPF1 regulator of nonsense transcripts homolog (yeast) (UPF1), mGFP tagged |
CNY 13,020.00 |
|
| RC208018L3 | Lenti ORF clone of Human UPF1 regulator of nonsense transcripts homolog (yeast) (UPF1), Myc-DDK-tagged |
CNY 14,072.00 |
|
| RC208018L4 | Lenti ORF clone of Human UPF1 regulator of nonsense transcripts homolog (yeast) (UPF1), mGFP tagged |
CNY 13,020.00 |
|
| RG208018 | UPF1 (tGFP-tagged) - Human UPF1 regulator of nonsense transcripts homolog (yeast) (UPF1) |
CNY 12,260.00 |
|
| SC118343 | UPF1 (untagged)-Human UPF1 regulator of nonsense transcripts homolog (yeast) (UPF1) |
CNY 7,792.00 |
