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UPF1 (NM_002911) Human Untagged Clone
CAT＃: SC118343
UPF1 (untagged)-Human UPF1 regulator of nonsense transcripts homolog (yeast) (UPF1)
CNY 7,792.00
| Cited in 2 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | HUPF1; NORF1; pNORF1; RENT1; smg-2; UTF |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC118343 sequence for NM_002911 edited (data generated by NextGen Sequencing)
ATGAGCGTGGAGGCGTACGGGCCCAGCTCGCAGACTCTCACTTTCCTGGACACGGAGGAG GCCGAGCTGCTTGGCGCCGACACACAGGGCTCCGAGTTCGAGTTCACCGACTTTACTCTT CCTAGCCAGACGCAGACGCCCCCCGGCGGCCCCGGCGGCCCGGGCGGTGGCGGCGCGGGA GGCCCGGGCGGCGCGGGCGCGGGCGCTGCGGCGGGACAGCTCGACGCGCAGGTTGGGCCC GAAGGCATCCTGCAGAACGGGGCTGTGGACGACAGTGTAGCCAAGACCAGCCAGTTGTTG GCTGAGTTGAACTTCGAGGAAGATGAAGAAGACACCTATTACACGAAGGACCTCCCCATA CACGCCTGCAGTTACTGTGGAATACACGATCCTGCCTGCGTGGTTTACTGTAATACCAGC AAGAAGTGGTTCTGCAACGGACGTGGAAATACTTCTGGCAGCCACATTGTAAATCACCTT GTGAGGGCAAAATGCAAAGAGGTGACCCTGCACAAGGACGGGCCCCTGGGGGAGACAGTC CTGGAGTGCTACAACTGCGGCTGTCGCAACGTCTTCCTCCTCGGCTTCATCCCGGCCAAA GCTGACTCAGTGGTGGTGCTGCTGTGCAGGCAGCCCTGTGCCAGCCAGAGCAGCCTCAAG GACATCAACTGGGACAGCTCGCAGTGGCAGCCGCTGATCCAGGACCGCTGCTTCCTGTCC TGGCTGGTCAAGATCCCCTCCGAGCAGGAGCAGCTGCGGGCACGCCAGATCACGGCACAG CAGATCAACAAGCTGGAGGAGCTGTGGAAGGAAAACCCTTCTGCCACGCTGGAGGACCTG GAGAAGCCGGGGGTGGACGAGGAGCCGCAGCATGTCCTCCTGCGGTACGAGGACGCCTAC CAGTACCAGAACATATTCGGGCCCCTGGTCAAGCTGGAGGCCGACTACGACAAGAAGCTG AAGGAGTCCCAGACTCAAGATAACATCACTGTCAGGTGGGACCTGGGCCTTAACAAGAAG AGAATCGCCTACTTCACTTTGCCCAAGACTGACTCTGACATGCGGCTCATGCAGGGGGAT GAGATATGCCTGCGGTACAAAGGGGACCTTGCGCCCCTGTGGAAAGGGATCGGCCACGTC ATCAAGGTCCCTGATAATTATGGCGATGAGATCGCCATTGAGCTGCGGAGCAGCGTGGGT GCACCTGTGGAGGTGACTCACAACTTCCAGGTGGATTTTGTGTGGAAGTCGACCTCCTTT GACAGGATGCAGAGCGCATTGAAAACGTTTGCCGTGGATGAGACCTCGGTGTCTGGCTAC ATCTACCACAAGCTGTTGGGCCACGAGGTGGAGGACGTAATCATCAAGTGCCAGCTGCCC AAGCGCTTCACGGCGCAGGGCCTCCCCGACCTCAACCACTCCCAGGTTTATGCCGTGAAG ACTGTGCTGCAAAGACCACTGAGCCTGATCCAGGGCCCGCCAGGCACGGGGAAGACGGTG ACGTCGGCCACCATCGTCTACCACCTGGCCCGGCAAGGCAACGGGCCGGTGCTGGTGTGT GCTCCGAGCAACATCGCCGTGGACCAGCTAACGGAGAAGATCCACCAGACGGGGCTAAAG GTCGTGCGCCTCTGCGCCAAGAGCCGTGAGGCCATCGACTCCCCGGTGTCTTTTCTGGCC CTGCACAACCAGATCAGGAACATGGACAGCATGCCTGAGCTGCAGAAGCTGCAGCAGCTG AAAGACGAGACTGGGGAGCTGTCGTCTGCCGACGAGAAGCGGTACCGGGCCTTGAAGCGC ACCGCAGAGAGAGAGCTGCTGATGAACGCAGATGTCATCTGCTGCACATGTGTGGGCGCC GGTGACCCGAGGCTGGCCAAGATGCAGTTCCGCTCCATTTTAATCGACGAAAGCACCCAG GCCACCGAGCCGGAGTGCATGGTTCCCGTGGTCCTCGGGGCCAAGCAGCTGATCCTTGTA GGCGACCACTGCCAGCTGGGCCCAGTGGTGATGTGCAAGAAGGCGGCCAAGGCCGGGCTG TCACAGTCGCTCTTCGAGCGCCTGGTGGTGCTGGGCATCCGGCCCATCCGCCTGCAGGTC CAGTACCGGATGCACCCTGCACTCAGCGCCTTCCCATCCAACATCTTCTACGAGGGCTCC CTCCAGAATGGTGTCACTGCAGCGGATCGTGTGAAGAAGGGATTTGACTTCCAGTGGCCC CAACCCGATAAACCGATGTTCTTCTACGTGACCCAGGGCCAAGAGGAGATTGCCAGCTCG GGCACCTCCTACCTGAACAGGACCGAGGCTGCGAACGTGGAGAAGATCACCACGAAGTTG CTGAAGGCAGGCGCCAAGCCGGACCAGATTGGCATCATCACGCCCTACGAGGGCCAGCGC TCCTACCTGGTGCAGTACATGCAGTTCAGCGGCTCCCTGCACACCAAGCTCTACCAGGAG GTGGAGATCGCCAGTGTGGACGCCTTTCAGGGACGCGAGAAGGACTTCATCATCCTGTCC TGTGTGCGGGCCAACGAGCACCAAGGCATTGGCTTTTTAAATGACCCCAGGCGTCTGAAC GTGGCCCTGACCAGAGCAAGGTATGGCGTCATCATTGTGGGCAACCCGAAGGCACTATCA AAGCAGCCGCTCTGGAACCACCTGCTGAACTACTATAAGGAGCAGAAGGTGCTGGTGGAG GGGCCGCTCAACAACCTGCGTGAGAGCCTCATGCAGTTCAGCAAGCCACGGAAGCTGGTC AACACTATCAACCCGGGAGCCCGCTTCATGACCACAGCCATGTATGATGCCCGGGAGGCC ATCATCCCAGGCTCCGTCTATGATCGGAGCAGCCAGGGCCGGCCTTCCAGCATGTACTTC CAGACCCATGACCAGATTGGCATGATCAGTGCCGGCCCTAGCCACGTGGCTGCCATGAAC ATTCCCATCCCCTTCAACCTGGTCATGCCACCCATGCCACCGCCTGGCTATTTTGGACAA GCCAACGGGCCTGCTGCAGGGCGAGGCACCCCGAAAGGCAAGACTGGTCGTGGGGGACGC CAGAAGAACCGCTTTGGGCTTCCTGGACCCAGCCAGACTAACCTCCCCAACAGCCAAGCC AGCCAGGATGTGGCGTCACAGCCCTTCTCTCAGGGCGCCCTGACGCAGGGCTACATCTCC ATGAGCCAGCCTTCCCAGATGAGCCAGCCCGGCCTCTCCCAGCCGGAGCTGTCCCAGGAC AGTTACCTTGGTGACGAGTTTAAATCACAAATCGACGTGGCGCTCTCACAGGACTCCACG TACCAGGGAGAGCGGGCTTACCAGCATGGCGGGGTGACGGGGCTGTCCCAGTATTAA Clone variation with respect to NM_002911.3 >OriGene 5' read for NM_002911 unedited
CCGCGGAATTCGCACGAGGCCGGGGCGGGCGGTTTGGTCCTTTCCGGGCGCGCGGGGGCG ACAGCGGCAGCGACCCGAGGCCTGCGGCCTAGGCCTCAGCGCGGCGGCGGGCTCGAGTGC AGCGCGGAACCGGCCCGAGGGCCCTACCCGGAGGCACCATGAGCGTGGAGGCGTACGGGC CCAGCTCGCAGACTCTCACTTTCCTGGACACGGAGGAGGCCGAGCTGCTTGGCGCCGACA CACAGGGCTCCGAGTTCGAGTTCACCGACTTTACTCTTCCTAGCCAGACGCAGACGCCCC CCGGCGGCCCCGGCGGCCCGGGCGGTGGCGGCGCGGGAGGCCCGGGCGGCGCGGGCGCGG GCGCTGCGGCGGGACAGCTCGACGCGCAGGTTGGGCCCGAAGGCATCCTGCAGAACGGGG CTGTGGACGACAGTGTAGCCAAGACCAGCCAGTTGTTGGCTGAGTTGAACTTCGAGGAAG ATGAAGAAGACACCTATTACACGAAGGACCTCCCCATACACGCCTGCAGTTACTGTGGAA TACACGATCCTGCCTGCGTGGTTTACTGTAATACCAGCAAGAAGTGGTTCTGCAACGGAC GTGGAAATACTTCTGGCAGCCACATTGTAAATCACCTTGTGAGGGCAAAATGCAAAGAGG TGACCCTGCACAAGGACGGGGCCCCTGGGGGAGACAGTCCCTGGAGTGCTACAACTGGCG GCTGTCGCAACGTCTTTCTCCTCGGCTTCATCCCGGGCAAAGCTGACTCAATGGGTGGGG GCTGCTGTGCAGGCAACCCCTGTGCCAACCCGAGCAGCCTCAAAGGACATAACTGGGACA ACTCGCAGTGGCAGACCGGTGATCCAGGAACGCGGGTTCCTGTCCTGGGTTGGGCAAAGA ACCCA >OriGene 3' read for NM_002911 unedited
AACGTAGGGGAAACAGGTATCCGTTTTTTTTTATTTTTTATTACCCAGNATAAGATGCTG ATGGACTAAGCCACGTTGCTTAGCTCTTCCGCCGCCACCTTTTAATACTGGGACAGCCCC GTCACCCCGCCATGCTGGTAAGCCCGCTCTCCCTGGTACGTGGAGTCCTGTGAGAGCGCC ACGTCGATTTGTGATTTAAACTCGTCACCAAGGTAACTGTCCTGGGACAGCTCCGGCTGG GAGAGGCCGGGCTGGCTCATCTGGGAAGGCTGGCTCATGGAGATGTAGCCCTGCGTCAGG GCGCCCTGAGAGAAGGGCTGTGACGCCACATCCTGGCTGGCTTGGCTGTTGGGGAGGTTA GTCTGGCTGGGTCCAGGAAGCCCAAAGCGGTTCTTCTGGCGTCCCCCACGACCAGTCTTG CCTTTCGGGGTGCCTCGCCCTGCAGCAGGCCCGTTGGCTTGTCCAAAATAGCCAGGCGGT GGCATGGGTGGCATGACCAGGTTGAAGGGGATGGGAATGTTCATGGCAGCCACGTGGCTA GGGCCGGCACTGATCATGCCAATCTGGTCATGGGTCTGGAAGTACATGCTGGAAGGCCGG CCCTGGCTGCTCCGATCATAGACGGAGCCTGGGATGATGGCCTCCCGGGCATCATACATG GCTGTGGTCATGAAGCGGGCTCCCGGTTGATAGTGTTGACCAGCTTCCGTGGCTTGCTGA ACTGCATGAGGCTTTCACCGCAGTTGTTGAGCGGGCCCTTCAACAGCACCCTTTTGTTCC TTATAGTAGTTCAGCAGGTGGGTTTCAGAGCCGGCTGCTTTGTTAAGTGCTCTCGGTTGC CCCCAATGATGAACCCCTAACCTTGCTCTGGTCAGGGCCACCCTCAACCCCCTTGGGTTA TTTTAAAAAACCATGGCTCTGGGCTTTCTTGCCCCACAACGGGAAGGT |
| Restriction Sites | ECoRI-NOT |
| ACCN | NM_002911 |
| Insert Size | 4000 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002911.2, NP_002902.2 |
| RefSeq Size | 5300 bp |
| RefSeq ORF | 3357 bp |
| Locus ID | 5976 |
| UniProt ID | Q92900 |
| Protein Families | Druggable Genome |
| Gene Summary | This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located only in the cytoplasm. When translation ends, it interacts with the protein that is a functional homolog of yeast Upf2p to trigger mRNA decapping. Use of multiple polyadenylation sites has been noted for this gene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014] Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. It encodes isoform 2, which is shorter than isoform 1. |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
The Zinc-Finger Antiviral Protein ZAP Inhibits LINE and Alu Retrotransposition
,Moldovan, JB;Moran, JV;,
PLoS Genet.
,PubMed ID 25951186
[UPF1]
|
|
A luciferase reporter assay to investigate the differential selenium-dependent stability of selenoprotein mRNAs.
,null,
The Journal of nutritional biochemistry
,PubMed ID 22209284
[UPF1]
|
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208018 | UPF1 (Myc-DDK-tagged)-Human UPF1 regulator of nonsense transcripts homolog (yeast) (UPF1) |
CNY 11,672.00 |
|
| RC208018L1 | Lenti ORF clone of Human UPF1 regulator of nonsense transcripts homolog (yeast) (UPF1), Myc-DDK-tagged |
CNY 14,072.00 |
|
| RC208018L2 | Lenti ORF clone of Human UPF1 regulator of nonsense transcripts homolog (yeast) (UPF1), mGFP tagged |
CNY 13,020.00 |
|
| RC208018L3 | Lenti ORF clone of Human UPF1 regulator of nonsense transcripts homolog (yeast) (UPF1), Myc-DDK-tagged |
CNY 14,072.00 |
|
| RC208018L4 | Lenti ORF clone of Human UPF1 regulator of nonsense transcripts homolog (yeast) (UPF1), mGFP tagged |
CNY 13,020.00 |
|
| RG208018 | UPF1 (tGFP-tagged) - Human UPF1 regulator of nonsense transcripts homolog (yeast) (UPF1) |
CNY 12,260.00 |
|
| SC323999 | UPF1 (untagged)-Human UPF1 regulator of nonsense transcripts homolog (yeast) (UPF1) |
CNY 7,792.00 |
