NIM1K (NM_153361) Human Untagged Clone

CAT#: SC323705

NIM1 (untagged)-Kinase deficient mutant (K103M) of Human serine/threonine-protein kinase NIM1 (MGC42105)



  "NM_153361" in other vectors (7)

CNY 7,220.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
Rabbit Polyclonal Anti-NIM1 Antibody
    • 100 ul

CNY 5,250.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms NIM1
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_153361, the custom clone sequence may differ by one or more nucleotides


ATGACTGCAGTGTATATGAATGGAGGTGGCCTGGTGAACCCCCACTATGCCCGGTGGGATCGGCGCGACA
GTGTAGAAAGTGGCTGTCAGACCGAGAGTAGCAAGGAGGGTGAGGAGGGACAGCCCCGCCAGCTGACGCC
CTTCGAGAAACTGACACAGGACATGTCCCAGGATGAGAAGGTGGTGAGGGAGATCACGCTGGGGAAACGG
ATAGGCTTCTACCGAATTCGAGGGGAAATCGGAAGTGGAAACTTCTCCCAAGTGAAGCTTGGGATTCACT
CCCTAACCAAAGAAAAGGTGGCCATTAAGATCCTGGACAAGACCAAGTTAGACCAGAAAACCCAGAGGCT
ACTATCCCGAGAAATCTCCAGCATGGAAAAGCTGCACCATCCCAACATCATCCGCCTTTACGAAGTGGTG
GAGACCCTATCCAAGCTGCACTTGGTGATGGAGTATGCAGGGGGTGGGGAGCTCTTCGGAAAAATTAGCA
CTGAGGGGAAGCTCTCTGAACCAGAAAGCAAGCTCATCTTCTCCCAGATTGTGTCTGCCGTGAAGCACAT
GCATGAAAACCAAATTATTCATAGAGATCTGAAAGCAGAAAATGTATTCTATACCAGTAATACTTGTGTG
AAGGTGGGCGATTTTGGATTCAGCACAGTAAGCAAAAAAGGTGAAATGCTGAACACTTTCTGTGGGTCTC
CTCCCTACGCTGCGCCTGAACTCTTCCGGGACGAGCACTACATCGGCATTTACGTGGATATCTGGGCCTT
GGGGGTGCTTTTGTACTTCATGGTGACTGGCACCATGCCATTTCGGGCAGAAACCGTGGCCAAACTAAAA
AAGAGCATCCTCGAGGGCACATACAGTGTACCGCCGCACGTGTCAGAGCCCTGCCACCGACTCATCCGAG
GAGTCCTTCAGCAGATCCCCACGGAGAGGTACGGAATCGACTGCATCATGAATGATGAATGGATGCAAGG
GGTGCCATACCCTACACCTTTGGAACCTTTCCAACTGGATCCCAAACATTTGTCGGAAACCAGCACTCTC
AAGGAAGAAGAAAATGAGGTCAAAAGCACTTTAGAACATTTGGGCATTACAGAAGAGCATATTCGAAATA
ACCAAGGGAGAGATGCTCGCAGCTCAATCACAGGGGTCTATAGAATTATTTTACATAGAGTCCAAAGGAA
GAAGGCTTTGGAAAGTGTCCCAGTCATGATGCTACCAGACCCTAAAGAAAGAGACCTCAAAAAAGGGTCC
CGTGTCTACAGAGGGATAAGACACACATCCAAATTTTGCTCGATTTTATAA


>OriGene 5' read for NM_153361 unedited
TCCGCCCGTCGAGCAACGGGCGGTAGGCGCTGTACGGATGGGAGGTCTATATAAGCAGAGCTCGTTTAGT
GAACCGTCAGAATCTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGTGCCGGTCAGG
TCGGCCGCCCCTGACAGCTCCGGGAGCCTCAAGCGCGACAGCGGCGCCCTCACCTCGGGACATCCACACA
CCGACCGCTCCTGCTCCAGAGGCAACAACCCAGCGCGCCTAGCCTGGCGCCGTGCAGCGAAGCCCAAGAG
CTGGCCTCGCCACGAAGAGATGGGGTCTCACTGTGTTGCCTAGGCTGGTCTTGAGCTCCTGGACTCAAAC
CATCCACCCGTCTCGGTCTCCCAAAGTGCTGGGATTATAGGTGTGAGCCACCACAACCAGCCTATTTTCT
TTTTAAAGGTTGAACCAGCCAAATTTTCGAGACAGCTCACGGCTTAGAGGAAGGTTCATCTAAATAAAGG
CCGGCTAAAGTGACATTGCAGGGATTAAATCCTTCTTTGGCTGCCTGTGTGACCAGAAGGCTTATTTGCA
AGTTTCTTCTTTCCTGGGGTCCAGATTATTAGGTCTCCAGCGCCCTGCAGCTTGACAGAAAGAGAAGCAT
GAAATGAAGGTCAGAGATGAGATCCCGCAGCAGGGACGTGGGGGCCTCCCAGGGGCATTTACGCACCAGA
GTGCAAGATTCTCTGGCCATCAAGGGAAATAGCAAACAGAAGCCTTTGTCCTGGGGCACAGCCACCTACC
ACAAAGCATCAGACTCCACGTCTGGCCAGAAAGTTCCTGGAGTCCCATCAGCCAGTGGGTATGTACATGT
GCCTAATTGTACAGCTAGAGCCTGCAAGTTCAACGTGAGGGAGGTGGCAAATGTCTGAAGTGAAGCGAGC
AGCTCTGGCTGGCTGGCAGAACTCACCTACCACGTCACTGGCTTCCTTCTCACTTAGAGGC
>SC323705 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
TACTCTTCGCTAGWGGAGACGTGAGCCCCCGTGGACGATGACTGCAGTGTATATGAATGGAGGTGGCCTG
GTGAACCCCCACTATGCCCGGTGGGATCGGCGCGACAGTGTAGAAAGTGGCTGTCAGACCGAGAGTAGCA
AGGAGGGTGAGGAGGGACAGCCCCGCCAGCTGACGCCCTTCGAGAAACTGACACAGGACATGTCCCAGGA
TGAGAAGGTGGTGAGGGAGATCACGCTGGGGAAACGGATAGGCTT
Restriction Sites Please inquire     
ACCN NM_153361
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_153361.2, NP_699192.1
RefSeq Size 2326 bp
RefSeq ORF 1311 bp
Locus ID 167359
UniProt ID Q8IY84
Protein Families Druggable Genome, Protein Kinase
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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