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RSK4 (RPS6KA6) (NM_014496) Human Untagged Clone
CAT＃: SC323689
RPS6KA6 (untagged)-Kinase deficient mutant (K455M) of Human ribosomal protein S6 kinase, 90kDa, polypeptide 6 (RPS6KA6)
CNY 5,464.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | p90RSK6; PP90RSK4; RSK-4; RSK4; S6K-alpha-6 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323689 sequence for NM_014496 edited (data generated by NextGen Sequencing)
ATGCTACCATTCGCTCCTCAGGACGAGCCCTGGGACCGAGAAATGGAAGTGTTCAGCGGC GGCGGCGCGAGCAGCGGCGAGGTAAATGGTCTTAAAATGGTTGATGAGCCAATGGAAGAG GGAGAAGCAGATTCTTGTCATGATGAAGGAGTTGTTAAAGAAATCCCTATTACTCATCAT GTTAAGGAAGGCTATGAGAAAGCAGATCCTGCACAGTTTGAGTTGCTCAAGGTTCTTGGT CAGGGGTCATTTGGAAAGGTTTTTCTTGTTAGAAAGAAGACCGGTCCTGATGCTGGGCAG CTCTATGCAATGAAGGTGTTAAAAAAAGCCTCTTTAAAAGTTCGAGACAGAGTTCGGACA AAGATGGAGAGGGATATACTGGTGGAAGTAAATCATCCATTTATTGTCAAATTGCACTAT GCCTTTCAGACTGAAGGGAAACTGTACTTAATACTGGATTTTCTCAGGGGAGGAGATGTT TTCACAAGATTATCCAAAGAGGTTCTGTTTACAGAGGAAGATGTGAAATTCTACCTCGCA GAACTGGCCCTTGCTTTGGATCATCTGCACCAATTAGGAATTGTTTATAGAGACCTGAAG CCAGAAAACATTTTGCTTGATGAAATAGGACATATCAAATTAACAGATTTTGGACTCAGC AAGGAGTCAGTAGATCAAGAAAAGAAGGCTTACTCATTTTGTGGTACAGTAGAGTATATG GCTCCTGAAGTAGTAAATAGGAGAGGCCATTCCCAGAGTGCTGATTGGTGGTCATATGGT GTTCTTATGTTTGAAATGCTTACTGGTACTCTGCCATTTCAAGGTAAAGACAGAAATGAG ACCATGAATATGATATTAAAAGCAAAACTTGGAATGCCTCAATTTCTTAGTGCTGAAGCA CAAAGTCTTCTAAGGATGTTATTCAAAAGGAATCCAGCAAATAGATTGGGATCAGAAGGA GTTGAAGAAATCAAAAGACATCTGTTTTTTGCAAATATTGACTGGGATAAATTATATAAA AGAGAAGTTCAACCTCCTTTCAAACCTGCTTCTGGAAAACCAGATGATACTTTTTGTTTT GATCCTGAATTTACTGCAAAAACACCTAAAGATTCTCCCGGTTTGCCAGCCAGTGCAAAT GCTCATCAGCTCTTCAAAGGATTCAGCTTTGTTGCAACTTCTATTGCAGAAGAATATAAA ATCACTCCTATCACAAGTGCAAATGTATTACCAATTGTTCAGATAAATGGAAATGCTGCA CAATTTGGTGAAGTATATGAATTGAAGGAGGATATTGGTGTTGGCTCCTACTCTGTTTGC AAGCGATGCATACATGCAACTACCAACATGGAATTTGCAGTGATGATCATTGACAAAAGT AAGCGAGACCCTTCAGAAGAGATTGAAATATTGATGCGCTATGGACAACATCCCAACATT ATTACTTTGAAGGATGTCTTTGATGATGGTAGATATGTTTACCTTGTTACGGATTTAATG AAAGGAGGAGAGTTACTTGACCGTATTCTCAAACAAAAATGTTTCTCGGAACGGGAGGCT AGTGATATACTATATGTAATAAGTAAGACAGTTGACTATCTTCATTGTCAAGGAGTTGTT CATCGTGATCTTAAACCTAGTAATATTTTATACATGGATGAATCAGCCAGTGCAGATTCA ATCAGGATATGTGATTTTGGGTTTGCAAAACAACTTCGAGGAGAAAATGGACTTCTCTTA ACTCCATGCTACACTGCAAACTTTGTTGCACCTGAGGTTCTTATGCAACAGGGATATGAT GCTGCTTGTGATATCTGGAGTTTAGGAGTCCTTTTTTACACAATGTTGGCTGGCTACACT CCATTTGCTAATGGCCCCAATGATACTCCTGAAGAGATACTGCTGCGTATAGGCAATGGA AAATTCTCTTTGAGTGGTGGAAACTGGGACAATATTTCAGACGGAGCAAAGGATTTGCTT TCCCATATGCTTCATATGGACCCACATCAGCGGTATACTGCTGAACAAATATTAAAGCAC TCATGGATAACTCACAGAGACCAGTTGCCAAATGATCAGCCAAAGAGAAATGATGTGTCA CATGTTGTTAAGGGAGCAATGGTTGCAACATACTCTGCCCTGACTCACAAGACCTTTCAA CCAGTCCTAGAGCCTGTAGCTGCTTCAAGCTTAGCCCAGCGACGGAGCATGAAAAAGCGA ACATCAACTGGCCTGTAA Clone variation with respect to NM_014496.4 1364 a=>t >OriGene 5' read for mutant NM_014496 unedited
ACCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCTCCGTGGAGTGG GCAGGTTTACCGAGGCGCTAGCAGGGGCTTTTACTACTCTTCAGTCGCCTCCCCAGCCCAGCTCCCAGCC TCTGAGAGACCCGGCGGCGGCGGCGGCGGGCCAGTTCAATAGACAGGATGCGCGGCCGCGCCAGCGGTAG GCGGCAGCTCCTGTTTGAGTGCTCCTGAAGGGAGATGCTACCATTCGCTCCTCAGGACGAGCCCTGGGAC CCGAGAAATGGGAAGTGTTCAGGCGGCCGGCGGGCGCGAGCAGCGGCGAAGGTAAAATGTCCTTAAAATG TTTGATGAGCCAAGGAAAAGGGGAGAAGGCAGAATTCTTGGTCTGAATGAAGGAGTGGTTAAGGAATTCC TTTTAACCAATATTTGTAAGGGAAGGTAATGAAAAGCAGTTCCGGCCCGTTTAGTTGGCTCAGGGTTTTT GTCAGGGTCTATTTGAAAGGTTTCTCTGTTAGAAAAAAACGGCCGTATGCTGGGCGCTTATGCTGAAAGT GTAAAAAACCTTTTAAATGTCGAGACAGATTGCGAAGAGAAGAAGGGAATCGTGGAAGATACTCACTATA TGGCAATTCCATGCCCTGAACTAGGAACTTGCTATACTGAATTCTAGGGAGAAGTTTCCAGATTCCAAGT TGTTCAAGAGATGAAATCTCGAATGCTGTCGTACTGCCATAGGATGGAGTCAACACACGTGTGGAAAGAA CAATGAATTGGGCCAAGCGTGTCAACAA >SC323689 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
AGAGAGWTTGGTGTTGGCTCCTACTCTGTTTGCAAGCGATGCATACATGCAACTACCAACATGGAATTTG CAGTGATGATCATTGACAAAAGTAAGCGAGACCCTTCAGAAGAGATTGAAATATTGATGCGCTATGGACA ACATCCCAACATTATTACTTTGAAGGATGTCTTTGATGATGGTAGATATGTTTACCTTGTTACGGATTTA ATGAAAGGAGGAGAGTTACTTGACCGTATTCTCAAACAAAAATGT |
| Restriction Sites | Please inquire |
| ACCN | NM_014496 |
| Insert Size | 5000 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_014496.1, NP_055311.1 |
| RefSeq Size | 2640 bp |
| RefSeq ORF | 2238 bp |
| Locus ID | 27330 |
| UniProt ID | Q9UK32 |
| Domains | pkinase, S_TK_X, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Long-term potentiation, MAPK signaling pathway, mTOR signaling pathway, Neurotrophin signaling pathway, Oocyte meiosis, Progesterone-mediated oocyte maturation |
| Gene Summary | This gene encodes a member of ribosomal S6 kinase family, serine-threonine protein kinases which are regulated by growth factors. The encoded protein may be distinct from other members of this family, however, as studies suggest it is not growth factor dependent and may not participate in the same signaling pathways. [provided by RefSeq, Jan 2010] Transcript Variant: This variant (2) differs in the 5' UTR and 5' coding region, compared to variant 1. The encoded isoform (2) is the same length as isoform 1, but has a distinct N-terminus. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC211545 | RPS6KA6 (Myc-DDK-tagged)-Human ribosomal protein S6 kinase, 90kDa, polypeptide 6 (RPS6KA6) |
CNY 5,456.00 |
|
| RC211545L1 | Lenti ORF clone of Human ribosomal protein S6 kinase, 90kDa, polypeptide 6 (RPS6KA6), Myc-DDK-tagged |
CNY 7,856.00 |
|
| RC211545L2 | Lenti ORF clone of Human ribosomal protein S6 kinase, 90kDa, polypeptide 6 (RPS6KA6), mGFP tagged |
CNY 9,690.00 |
|
| RC211545L3 | Lenti ORF clone of Human ribosomal protein S6 kinase, 90kDa, polypeptide 6 (RPS6KA6), Myc-DDK-tagged |
CNY 9,690.00 |
|
| RC211545L4 | Lenti ORF clone of Human ribosomal protein S6 kinase, 90kDa, polypeptide 6 (RPS6KA6), mGFP tagged |
CNY 9,690.00 |
|
| RG211545 | RPS6KA6 (tGFP-tagged) - Human ribosomal protein S6 kinase, 90kDa, polypeptide 6 (RPS6KA6) |
CNY 7,056.00 |
|
| SC114987 | RPS6KA6 (untagged)-Human ribosomal protein S6 kinase, 90kDa, polypeptide 6 (RPS6KA6) |
CNY 5,464.00 |
|
| SC323492 | RPS6KA6 (untagged)-Kinase deficient mutant (K105M) of Human ribosomal protein S6 kinase, 90kDa, polypeptide 6 (RPS6KA6) |
CNY 5,464.00 |
|
| SC323631 | RPS6KA6 (untagged)-Kinase deficient mutant (K455M) of Human ribosomal protein S6 kinase, 90kDa, polypeptide 6 (RPS6KA6) |
CNY 5,464.00 |
