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ZAP70 (NM_001079) Human Untagged Clone
CAT＃: SC323675
ZAP70 (untagged)-Kinase deficient mutant (K369M) of Human zeta-chain (TCR) associated protein kinase 70kDa (ZAP70), transcript variant 1
CN¥ 4,616.00
CN¥ 7,220.00
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Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ADMIO2; IMD48; SRK; STCD; STD; TZK; ZAP-70 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323675 sequence for NM_001079 edited (data generated by NextGen Sequencing)
ATGCCAGACCCCGCGGCGCACCTGCCCTTCTTCTACGGCAGCATCTCGCGTGCCGAGGCC GAGGAGCACCTGAAGCTGGCGGGCATGGCGGACGGGCTCTTCCTGCTGCGCCAGTGCCTG CGCTCGCTGGGCGGCTATGTGCTGTCGCTCGTGCACGATGTGCGCTTCCACCACTTTCCC ATCGAGCGCCAGCTCAACGGCACCTACGCCATTGCCGGCGGCAAAGCGCACTGTGGACCG GCAGAGCTCTGCGAGTTCTACTCGCGCGACCCCGACGGGCTGCCCTGCAACCTGCGCAAG CCGTGCAACCGGCCGTCGGGCCTCGAGCCGCAGCCGGGGGTCTTCGACTGCCTGCGAGAC GCCATGGTGCGTGACTACGTGCGCCAGACGTGGAAGCTGGAGGGCGAGGCCCTGGAGCAG GCCATCATCAGCCAGGCCCCGCAGGTGGAGAAGCTCATTGCTACGACGGCCCACGAGCGG ATGCCCTGGTACCACAGCAGCCTGACGCGTGAGGAGGCCGAGCGCAAACTTTACTCTGGG GCGCAGACCGACGGCAAGTTCCTGCTGAGGCCGCGGAAGGAGCAGGGCACATACGCCCTG TCCCTCATCTATGGGAAGACGGTGTACCACTACCTCATCAGCCAAGACAAGGCGGGCAAG TACTGCATTCCCGAGGGCACCAAGTTTGACACGCTCTGGCAGCTGGTGGAGTATCTGAAG CTGAAGGCGGACGGGCTCATCTACTGCCTGAAGGAGGCCTGCCCCAACAGCAGTGCCAGC AACGCCTCAGGGGCTGCTGCTCCCACACTCCCAGCCCACCCATCCACGTTGACTCATCCT CAGAGACGAATCGACACCCTCAACTCAGATGGATACACCCCTGAGCCAGCACGCATAACG TCCCCAGACAAACCGCGGCCGATGCCCATGGACACGAGCGTGTATGAGAGCCCCTACAGC GACCCAGAGGAGCTCAAGGACAAGAAGCTCTTCCTGAAGCGCGATAACCTCCTCATAGCT GACATTGAACTTGGCTGCGGCAACTTTGGCTCAGTGCGCCAGGGCGTGTACCGCATGCGC AAGAAGCAGATCGACGTGGCCATCATGGTGCTGAAGCAGGGCACGGAGAAGGCAGACACG GAAGAGATGATGCGCGAGGCGCAGATCATGCACCAGCTGGACAACCCCTACATCGTGCGG CTCATTGGCGTCTGCCAGGCCGAGGCCCTCATGCTGGTCATGGAGATGGCTGGGGGCGGG CCGCTGCACAAGTTCCTGGTCGGCAAGAGGGAGGAGATCCCTGTGAGCAATGTGGCCGAG CTGCTGCACCAGGTGTCCATGGGGATGAAGTACCTGGAGGAGAAGAACTTTGTGCACCGT GACCTGGCGGCCCGCAACGTCCTGCTGGTTAACCGGCACTACGCCAAGATCAGCGACTTT GGCCTCTCCAAAGCACTGGGTGCCGACGACAGCTACTACACTGCCCGCTCAGCAGGGAAG TGGCCGCTCAAGTGGTACGCACCCGAATGCATCAACTTCCGCAAGTTCTCCAGCCGCAGC GATGTCTGGAGCTATGGGGTCACCATGTGGGAGGCCTTGTCCTACGGCCAGAAGCCCTAC AAGAAGATGAAAGGGCCGGAGGTCATGGCCTTCATCGAGCAGGGCAAGCGGATGGAATGC CCACCAGAGTGTCCACCCGAACTGTACGCACTCATGAGTGACTGCTGGATCTACAAGTGG GAGGATCGCCCCGACTTCCTGACCGTGGAGCAGCGCATGCGAGCCTGTTACTACAGCCTG GCCAGCAAGGTGGAAGGGCCCCCAGGCAGCACACAGAAGGCTGAGGCTGCCTGTGCCTGA Clone variation with respect to NM_001079.3 1106 a=>t;1677 g=>a >OriGene 5' read for mutant NM_001079 unedited
ACCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGGAGCGGCAGGGCCTC CCTCTCAGGCGGGGGGCTTGGGCTGCAGGGAAATCCAAACCGGCTTTGTAAGCCCCGATTCCTCACCCAG AACCGGCTCTCCATTGGCATTGGGACCAGAGACCCCGCAAGTGGCCTGTTTGCCTGGACATCCACCTGTA CGTCCCCAGGTTTCGGGAGGCCCAGGGGCGATGCCAGACCCCGCGGCGCACCTGCCCTTCTTCTAGGGCA GCATCTCGCGTGCCGAGGCCGAGGAGCACCTGAAGCTGGCGGGCATGGCGGACGGGCTCTTCCTGCTGCG CCAGTGCCTGCGCTCGCTGGGCGGCTATGTGCTGTCGCTCGTGCACGATGTGCGCTTCCACCACTTTCCC ATCGGAGCGCAGCTCAACGGCACCTACGCATTGCCGGCGGCAAAGCCGCACTGGTGGACCGGCAGAGAGC TTCTGCAAGTTCTAACTGGCCGGACCCGGACGGGCTGGCCTTGCAACTGGCCCAGGCTGTGCAACGGCGT CGGGCCTTAAGCGGAAACGGGGGTCTCCTCGCAACTGCCTGCAGAACGCCATGGGGCGGTGACACTACTG TGCCTAACTGTGAAACCTGGGAGGGCAAGGCCCTTGGGACAAGGCCTATTATACACAGGCCCGAGAGTGT GGGAAACTCTAGTGCTACAGGGCCTAGACCGGATTCTGTGTACAACAGACCGTAGACTTGGAGAGCCAAG CCAACTATACCTGGGGCAACACTGCAGATCCGTGAGAGCTCGAAGAGTACAGACCATATCCGTGCTATAT GGACCGGTGTACCCATATCTACTTAAAACGGTCATATCGTTCGAGACAAATGGCATCGCATCTGGTGAGC CTA >SC323675 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
TACTGGCTGCGGCACTTTGGCTCAGTGCGCCAGGGCGTGTACCGCATGCGCAAGTATGGCCGCCCCTGCC GTGGTGGGAGCACCGCCGCCTGGGGCAGAGGGGAGTGGCTTCACCGGGCTGTGGGACGGGAGCCGGGATG TCTGTCTCACAGCAGTTTGCCTGGGAAACAGACTCTGGGGCAGGACGTTGCACGCTGGAGGATTCCCTGA GAGAGCTCGGGACACCTGACGGGGGTGGGATGGGGCCTGGGCAGG |
| Restriction Sites | Please inquire |
| ACCN | NM_001079 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001079.3, NP_001070.2 |
| RefSeq Size | 2450 bp |
| RefSeq ORF | 1860 bp |
| Locus ID | 7535 |
| UniProt ID | P43403 |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Natural killer cell mediated cytotoxicity, Primary immunodeficiency, T cell receptor signaling pathway |
| Gene Summary | This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208838 | ZAP70 (Myc-DDK-tagged)-Human zeta-chain (TCR) associated protein kinase 70kDa (ZAP70), transcript variant 1 |
CN¥ 6,912.00 |
|
| RC208838L1 | Lenti ORF clone of Human zeta-chain (TCR) associated protein kinase 70kDa (ZAP70), transcript variant 1, Myc-DDK-tagged |
CN¥ 9,312.00 |
|
| RC208838L2 | Lenti ORF clone of Human zeta-chain (TCR) associated protein kinase 70kDa (ZAP70), transcript variant 1, mGFP tagged |
CN¥ 5,890.00 |
|
| RC208838L3 | Lenti ORF clone of Human zeta-chain (TCR) associated protein kinase 70kDa (ZAP70), transcript variant 1, Myc-DDK-tagged |
CN¥ 5,890.00 |
|
| RC208838L4 | Lenti ORF clone of Human zeta-chain (TCR) associated protein kinase 70kDa (ZAP70), transcript variant 1, mGFP tagged |
CN¥ 9,312.00 |
|
| RG208838 | ZAP70 (tGFP-tagged) - Human zeta-chain (TCR) associated protein kinase 70kDa (ZAP70), transcript variant 1 |
CN¥ 8,512.00 |
|
| SC310215 | ZAP70 (untagged)-Human zeta-chain (TCR) associated protein kinase 70kDa (ZAP70), transcript variant 1 |
CN¥ 4,616.00 |
