IRAKM (IRAK3) (NM_007199) Human Untagged Clone
CAT#: SC323662
IRAK3 (untagged)-Kinase deficient mutant (K192M) of Human interleukin-1 receptor-associated kinase 3 (IRAK3), transcript variant 1
CNY 4,448.00
CNY 7,220.00
Cited in 1 publication. |
Product images
![](https://cdn.origene.com/img/defaults-img.jpg)
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | ASRT5; IRAKM |
Vector | pCMV6-XL4 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF within SC323662 sequence for NM_007199 edited (data generated by NextGen Sequencing)
ATGGCGGGGAACTGTGGGGCCCGCGGCGCGCTGTCGGCGCACACGCTGCTGTTCGACCTG CCGCCCGCGCTGCTCGGAGAGCTCTGCGCTGTTCTGGACAGCTGCGACGGCGCGCTGGGC TGGCGCGGCCTGGCAGAGAGACTTTCAAGCAGCTGGCTGGATGTTCGTCATATTGAAAAG TATGTAGACCAAGGTAAAAGTGGAACAAGAGAATTACTTTGGTCCTGGGCACAGAAAAAC AAGACCATCGGTGACCTTTTACAGGTCCTCCAGGAGATGGGACATCGTCGAGCTATTCAT TTAATTACAAACTATGGAGCAGTGTTGAGTCCTTCAGAGAAGAGTTATCAGGAAGGTGGA TTTCCAAATATATTATTCAAGGAAACAGCCAATGTCACCGTGGATAATGTTCTTATTCCT GAACATAATGAAAAAGGAGTACTGCTTAAATCTTCCATCAGCTTTCAAAATATCATAGAA GGAACTAGAAATTTCCACAAAGACTTCCTAATTGGAGAAGGAGAGATTTTTGAGGTATAC AGAGTGGAGATTCAAAACCTAACATATGCTGTCATGTTATTTAAACAGGAGAAAAAAATG CAGTGTAAGAAGCATTGGAAGAGGTTTTTATCTGAGCTTGAAGTTTTACTACTGTTTCAT CACCCAAACATACTAGAGTTGGCTGCATATTTTACAGAGACTGAGAAGTTCTGTCTGATT TATCCATACATGAGAAATGGAACACTTTTTGACAGATTGCAGTGTGTAGGTGACACGGCC CCACTCCCTTGGCACATTCGAATCGGTATATTAATAGGAATATCCAAAGCCATTCACTAC CTGCACAACGTTCAACCATGCTCGGTCATCTGTGGCAGTATATCAAGTGCAAACATCCTT TTGGATGATCAGTTTCAACCCAAACTAACTGATTTTGCCATGGCACACTTCCGGTCCCAC CTAGAACATCAGAGTTGTACCATAAATATGACCAGCAGCAGCAGTAAACATCTGTGGTAC ATGCCAGAAGAGTACATCAGACAGGGGAAACTTTCCATTAAAACAGATGTCTACAGCTTT GGAATTGTAATAATGGAAGTTCTAACAGGATGTAGAGTAGTGTTAGATGATCCAAAACAT ATCCAGCTGCGGGATCTCCTTAGAGAATTGATGGAGAAGAGAGGCCTGGATTCATGTCTC TCATTTCTAGATAAGAAAGTGCCTCCCTGCCCTCGGAATTTCTCTGCCAAGCTCTTCTGT TTGGCAGGCCGGTGTGCTGCAACGCGGGCAAAGTTAAGACCATCAATGGATGAAGTTTTA AATACTCTTGAAAGTACTCAAGCCAGCTTGTATTTTGCTGAAGATCCTCCCACATCACTA AAGTCCTTCAGGTGTCCTTCTCCTCTATTCCTGGAGAATGTACCAAGTATTCCAGTGGAA GATGATGAAAGCCAGAATAACAATTTACTACCTTCTGATGAAGGCCTGAGGATAGACAGA ATGACTCAGAAAACTCCTTTTGAATGCAGCCAGTCTGAGGTTATGTTTCTGAGCTTGGAC AAAAAGCCAGAGAGCAAGAGAAATGAGGAAGCTTGCAACATGCCCAGTTCTTCTTGTGAA GAAAGTTGGTTCCCAAAGTATATAGTTCCATCCCAGGACTTAAGGCCCTATAAGGTAAAT ATAGATCCTTCTTCAGAAGCTCCAGGGCATTCTTGCAGGAGCAGGCCAGTGGAGAGCAGC TGTTCCTCCAAATTTTCCTGGGATGAATATGAACAGTACAAAAAAGAATAA Clone variation with respect to NM_007199.2 439 a=>g;575 a=>t;576 a=>g >OriGene 5' read for mutant NM_007199 unedited
ACCGCCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGA ACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGGGCCTGTCGCAGGC GTGCAGGGACCTGGACTCCGCCTCGTCCCCGGGGCTCGGGCAGCCGAGCCATGGCGGGGAACTGTGGGGC CCGCGGCGCGCTGTCGGCGCACACGCTGCTGTTCGACCTGCCGCCCGCGCTGCTCGGAGAGCTCTGCGCT GTTCTGGACAGCTGCGACGGGCGCGCTGGGCTGGGCGCGGCCTGGCAGAGAGAACTTTTCAAGCAGGCTG TGGCGGGGATGGTTCGTCCATATTGAAAAAGTATGGTAAGACACCAAGGGTTAAAGTGTGGACAAGAGAA AATAACCTTTGGTCCCTGGGGCCCAGAAAAAACAAGAACCATCGTTGAACCCTTTAACAGGGTCCCTCCC AGAAGAATGGACACATCGCCAAACTATTTTCTTTTATTTTCCAAACCAATGGGACCCTGGGTTGAATTCC TTTCAAAAAAAAATTTTTACGGGAGGGGGGGATTTCCCAAATATTTTTTTCAGGGGAACCCCCCAAGGGT CCCCGGGGGAAAAGTGTTTTATTTTCCGGCCCATAGGGAAGGGGGTCTGGGGTTTAACTTTTCCCCTCCC CTTTTTAAAAATTCTAAAAAGGGACCTAAAATTTTTCCACAAAAAAATCTCCAATTGGGGAAAAGGAAGA AATTTTTTTGGGGGTACCAAAGAGGGGAATATACAAACCCACCCCACATCTGGTCCCGGTGCGGTTTAAC ACACGGGAAGAAAATAGCATGTCGTTGTGAAGACACCTGTGGGAAGAGATGTGTTTCTCGGCATCGCATG TATTTTACGGATGTGCTGTACTCCCGCAACATCACTAGTGGTGTGCGTATATTATTCGACAGCAACGCA >SC323662 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
CCATGMGCAATGGGCGKAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCA GAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGGGCCTGTCGCAGGCGTGCAGG GACCTGGACTCCGCCTCGTCCCCGGGGCTCGGGCAGCCGAGCCATGGCGGGGAACTGTGGGGCCCGCGGC GCGCTGTCGGCGCACACGCTGCTGTTCGACCTGCCGCCCGCGCTG |
Restriction Sites | Please inquire |
ACCN | NM_007199 |
Insert Size | 2500 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_007199.1, NP_009130.1 |
RefSeq Size | 2288 bp |
RefSeq ORF | 1791 bp |
Locus ID | 11213 |
UniProt ID | Q9Y616 |
Domains | DEATH, pkinase, TyrKc, S_TKc |
Protein Families | Druggable Genome, Protein Kinase |
Protein Pathways | Apoptosis, Neurotrophin signaling pathway |
Gene Summary | This gene encodes a member of the interleukin-1 receptor-associated kinase protein family. Members of this family are essential components of the Toll/IL-R immune signal transduction pathways. This protein is primarily expressed in monocytes and macrophages and functions as a negative regulator of Toll-like receptor signaling. Mutations in this gene are associated with a susceptibility to asthma. Alternate splicing results in multiple transcript variants. [provided by RefSeq, May 2010] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
---|
The Opposite Effects of Acute and Chronic Alcohol on Lipopolysaccharide-Induced Inflammation Are Linked to IRAK-M in Human Monocytes
,Pranoti Mandrekar, Shashi Bala, Donna Catalano, Karen Kodys, and Gyongyi Szabo,
J. Immunol., Jul 2009; 183: 1320 - 1327.
[IRAK3]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC209245 | IRAK3 (Myc-DDK-tagged)-Human interleukin-1 receptor-associated kinase 3 (IRAK3), transcript variant 1 |
CNY 4,440.00 |
|
RC209245L1 | Lenti ORF clone of Human interleukin-1 receptor-associated kinase 3 (IRAK3), transcript variant 1, Myc-DDK-tagged |
CNY 6,840.00 |
|
RC209245L2 | Lenti ORF clone of Human interleukin-1 receptor-associated kinase 3 (IRAK3), transcript variant 1, mGFP tagged |
CNY 6,840.00 |
|
RC209245L3 | Lenti ORF clone of Human interleukin-1 receptor-associated kinase 3 (IRAK3), transcript variant 1, Myc-DDK-tagged |
CNY 6,840.00 |
|
RC209245L4 | Lenti ORF clone of Human interleukin-1 receptor-associated kinase 3 (IRAK3), transcript variant 1, mGFP tagged |
CNY 6,840.00 |
|
RG209245 | IRAK3 (tGFP-tagged) - Human interleukin-1 receptor-associated kinase 3 (IRAK3), transcript variant 1 |
CNY 6,040.00 |
|
SC115680 | IRAK3 (untagged)-Human interleukin-1 receptor-associated kinase 3 (IRAK3), transcript variant 1 |
CNY 4,448.00 |