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LYN (NM_002350) Human Untagged Clone
CAT＃: SC323651
LYN (untagged)-Kinase deficient mutant (K275M) of Human v-yes-1 Yamaguchi sarcoma viral related oncogene homolog (LYN), transcript variant 1
CNY 5,728.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | JTK8; p53Lyn; p56Lyn |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_002350 edited
GCCACCCCCGGCCCCGCGCCAGCAGCCCCTCGCCGCGCGTCCAGCGTTCCCGGCCAGCAG CCTCCCCATACGCAGGTCCTGCTGGGCCGCCCCGTCGCGCCCCCCACTCTGAACTCAAGT CACCGTGGAGCTCCGCCGCCCCGAAACTTTCACCGCGAGCGGGAAATATGGGATGTATAA AATCAAAAGGGAAAGACAGCTTGAGTGACGATGGAGTAGATTTGAAGACTCAACCAGTAC GTAATACTGAAAGAACTATTTATGTGAGAGATCCAACGTCCAATAAACAGCAAAGGCCAG TTCCAGAATCTCAGCTTTTACCTGGACAGAGGTTTCAAACTAAAGATCCAGAGGAACAAG GAGACATTGTGGTAGCCTTGTACCCCTATGATGGCATCCACCCGGACGACTTGTCTTTCA AGAAAGGAGAGAAGATGAAAGTCCTGGAGGAGCATGGAGAATGGTGGAAAGCAAAGTCCC TTTTAACAAAAAAAGAAGGCTTCATCCCCAGCAACTATGTGGCCAAACTCAACACCTTAG AAACAGAAGAGTGGTTTTTCAAGGATATAACCAGGAAGGACGCAGAAAGGCAGCTTTTGG CACCAGGAAATAGCGCTGGAGCTTTCCTTATTAGAGAAAGTGAAACATTAAAAGGAAGCT TCTCTCTGTCTGTCAGAGACTTTGACCCTGTGCATGGTGATGTTATTAAGCACTACAAAA TTAGAAGTCTGGATAATGGGGGCTATTACATCTCTCCACGAATCACTTTTCCCTGTATCA GCGACATGATTAAACATTACCAAAAGCAGGCAGATGGCTTGTGCAGAAGATTGGAGAAGG CTTGTATTAGTCCCAAGCCACAGAAGCCATGGGATAAAGATGCCTGGGAGATCCCCCGGG AGTCCATCAAGTTGGTGAAAAGGCTTGGCGCTGGGCAGTTTGGGGAAGTCTGGATGGGTT ACTATAACAACAGTACCAAGGTGGCTGTGATGACCCTGAAGCCAGGAACTATGTCTGTGC AAGCCTTCCTGGAAGAAGCCAACCTCATGAAGACCCTGCAGCATGACAAGCTCGTGAGGC TCTACGCTGTGGTCACCAGGGAGGAGCCCATTTACATCATCACCGAGTACATGGCCAAGG GCAGTTTGCTGGATTTCCTGAAGAGCGATGAAGGTGGCAAAGTGCTGCTTCCAAAGCTCA TTGACTTTTCTGCTCAGATTGCAGAGGGAATGGCATACATCGAGCGGAAGAACTACATTC ACCGGGACCTGCGAGCAGCTAATGTTCTGGTCTCCGAGTCACTCATGTGCAAAATTGCAG ATTTTGGCCTTGCTAGAGTAATTGAAGATAATGAGTACACAGCAAGGGAAGGTGCTAAGT TCCCTATTAAGTGGACGGCTCCAGAAGCAATCAACTTTGGATGTTTCACTATTAAGTCTG ATGTGTGGTCCTTTGGAATCCTCCTATACGAAATTGTCACCTATGGGAAAATTCCCTACC CAGGGAGAACTAATGCCGACGTGATGACCGCCCTGTCCCAGGGCTACAGGATGCCCCGTG TGGAGAACTGCCCAGATGAGCTCTATGACATTATGAAAATGTGCTGGAAAGAAAAGGCAG AAGAGAGACCAACGTTTGACTACTTACAGAGCGTCCTGGATGATTTCTACACAGCCACGG AAGGGCAATACCAGCAGCAGCCTTAGAGCACAGGGAGACCCGTCCATTTGGCAGGGGTGG CTGCCTCATTTAGAGAGGAAAAGTAACCATCACTGGTTGCACTTATGATTTCATGTGCGG GGATCATCTGCCGTGCCTGGATCCTGAAATAGAGGCTAAATTACTCAGGAAGAACACCCT CTAAATGGGAAAGTATTCTGTACTCTTAGATGGATTCTCCACTCAGTTGCAACTTGGACT TGTCCTCAGCAGCTGGTAATCTTGCTCTGCTTGACAACATCTGAGTGCAGCCGTTTGAGA AGAAAACATCTATTCTCTCCAAAAATGCACCCAACTAGCTCTATGTTTACAAATGGACAT AGGACTCAAAGTTTCAGAGACCATTGCAATGAATCCCCAATAATTGCAGAACTAAACTCA TTTATAAAGCTAAAATAACCGGATATATACATAGCATGACATTTCTTTGTGCTTTGGCTT ACTTGTTTAAAAAAAAAAAAAAAAAA >OriGene 5' read for mutant NM_002350 unedited
ACCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGCCACCCCCGGCCCCG CGCCAGCAGCCCCTCGCCGCGCGTCCAGCGTTCCCGGCCAGCAGCCTCCCCATACGCAGGTCCTGCTGGG CCGCCCCGTCGCGCCCCCCACTCTGAACTCAAGTCACCGTGGAGCTCCGCCGCCCCGAAACTTTCACCGC GAGCGGGAAATATGGGATGTATAAAATCAAAAGGGAAAGACAGCTTTGAGTGACGATGGAGTAGATTTGA AGACTCAACCAGTACGTAAATACTGAAAAGAAACTATTTTATGTTGAAGAAAATCAAACGTCCCAAATAA AACAGCAAAAGGCCCAGTTTCAGAGATTCTCAGGCTTTTTACCCTGGGAACAGGAAGGTTTCAAAACTTA AAGATATCCCGAGAGGAACCAAGGGAAACTTTGGGGTAAGGCTTGTGAACCCCTATTGAGTGGGACTCCA CCCCCGAGCGATTTTGCCTTTCCAGGAAGGAAGAAAGATGAAGTCTCTTGGAGGGACAGGGGAAAGGGGG AAGAGCAAATTCCCTTTACACAAAAGAGAGGGTTTCTCCCACAGCCTATGGGCCAAACTACACCCCTTAA AACAGAGAGTGTTTTTAGGGATATCCCCAAGAGACGCAAGGGCCTTTTGGACACGAGAAATAGCTGTGAG CTCTCATATGAGAATGGACACTTAAGAGGCTCTCTCGTGCTGCAGAACTTAGACCTGTCGATGAGGTTTA GACACTCAATTATATCTAGATAGGCGCTAATCTTCCGAAACACTTCGTACCCGGTTATATTTTCAAACGC AATGTGTGCAAAATGGAAAGACGGTTA >SC323673 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
ARCTGGGRACCCCGGGAGTCATCAAGTTGGTGAAAAGGCTTGGCGCTGGGCAGTTTGGGGAAGTCTGGAT GGGTTACTATAACAACAGTACCAAGGTGGCTGTGATGACCCTGAAGCCAGGAACTATGTCTGTGCAAGCC TTCCTGGAAGAAGCCAACCTCATGAAGACCCTGCAGCATGACAAGCTCGTGAGGCTCTACGCTGTGGTCA CCAGGGAGGAGCCCATTTACATCATCACCGAGTACATGGCCAAGG |
| Restriction Sites | Please inquire |
| ACCN | NM_002350 |
| Insert Size | 3000 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002350.1, NP_002341.1 |
| RefSeq Size | 2298 bp |
| RefSeq ORF | 1539 bp |
| Locus ID | 4067 |
| UniProt ID | P07948 |
| Domains | pkinase, SH2, TyrKc, SH3, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | B cell receptor signaling pathway, Chemokine signaling pathway, Epithelial cell signaling in Helicobacter pylori infection, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Long-term depression |
| Gene Summary | This gene encodes a tyrosine protein kinase, which maybe involved in the regulation of mast cell degranulation, and erythroid differentiation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2011] Transcript Variant: This variant (1) encodes the longer isoform (A). |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC212851 | LYN (Myc-DDK-tagged)-Human v-yes-1 Yamaguchi sarcoma viral related oncogene homolog (LYN), transcript variant 1 |
CNY 5,720.00 |
|
| RC212851L1 | Lenti-ORF clone of LYN (Myc-DDK-tagged)-Human v-yes-1 Yamaguchi sarcoma viral related oncogene homolog (LYN), transcript variant 1 |
CNY 8,120.00 |
|
| RC212851L2 | Lenti-ORF clone of LYN (mGFP-tagged)-Human v-yes-1 Yamaguchi sarcoma viral related oncogene homolog (LYN), transcript variant 1 |
CNY 8,120.00 |
|
| RC212851L3 | Lenti-ORF clone of LYN (Myc-DDK-tagged)-Human v-yes-1 Yamaguchi sarcoma viral related oncogene homolog (LYN), transcript variant 1 |
CNY 6,460.00 |
|
| RC212851L4 | Lenti-ORF clone of LYN (mGFP-tagged)-Human v-yes-1 Yamaguchi sarcoma viral related oncogene homolog (LYN), transcript variant 1 |
CNY 8,120.00 |
|
| RG212851 | LYN (tGFP-tagged) - Human v-yes-1 Yamaguchi sarcoma viral related oncogene homolog (LYN), transcript variant 1 |
CNY 7,320.00 |
|
| SC108492 | LYN (untagged)-Human v-yes-1 Yamaguchi sarcoma viral related oncogene homolog (LYN), transcript variant 1 |
CNY 5,728.00 |
|
| SC323673 | LYN (untagged)-Kinase deficient mutant (K275M) of Human v-yes-1 Yamaguchi sarcoma viral related oncogene homolog (LYN), transcript variant 1 |
CNY 5,728.00 |
