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MEK5 (MAP2K5) (NM_002757) Human Untagged Clone
CAT＃: SC323639
MAP2K5 (untagged)-Kinase deficient mutant (K195M) of Human mitogen-activated protein kinase kinase 5 (MAP2K5), transcript variant B
CNY 3,656.00
CNY 7,030.00
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热销推荐Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | HsT17454; MAPKK5; MEK5; PRKMK5 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_002757, the custom clone sequence may differ by one or more nucleotides
ATGCTGTGGCTAGCCCTTGGCCCCTTTCCTGCCATGGAGAACCAGGTGCTGGTAATTCGCATCAAGATCC CAAATAGTGGCGCGGTGGACTGGACAGTGCACTCCGGGCCGCAGTTACTCTTCAGGGATGTGCTGGATGT GATAGGCCAGGTTCTGCCTGAAGCAACAACTACAGCATTTGAATATGAAGATGAAGATGGTGATCGAATT ACAGTGAGAAGTGATGAGGAAATGAAGGCAATGCTGTCATATTATTATTCCACAGTAATGGAACAGCAAG TAAATGGACAGTTAATAGAGCCTCTGCAGATATTTCCAAGAGCCTGCAAGCCTCCTGGGGAACGGAACAT ACATGGCCTGAAGGTGAATACTCGGGCCGGACCCTCTCAACACAGCAGCCCAGCAGTCTCAGATTCACTT CCAAGCAATAGCTTAAAGAAGTCTTCTGCTGAACTGAAAAAAATACTAGCCAATGGCCAGATGAATGAAC AAGACATACGATATCGGGACACTCTTGGTCATGGCAACGGAGGCACAGTCTACAAAGCATATCATGTCCC GAGTGGGAAAATATTAGCTGTAAAGGTCATACTACTAGATATTACACTGGAACTTCAGAAGCAAATTATG TCTGAATTGGAAATTCTTTATAAGTGCGATTCATCATATATCATTGGATTTTATGGAGCATTTTTTGTAG AAAACAGGATTTCAATATGTACAGAATTCATGGATGGGGGATCTTTGGATGTATATAGGAAAATGCCAGA ACATGTCCTTGGAAGAATTGCAGTAGCAGTTGTTAAAGGCCTTACTTATTTGTGGAGTTTAAAGATTTTA CATAGAGACGTGAAGCCCTCCAATATGCTAGTAAACACAAGAGGACAGGTTAAGCTGTGTGATTTTGGAG TTAGCACTCAGCTGGTGAATTCTATAGCCAAGACGTATGTTGGAACAAATGCTTATATGGCGCCTGAAAG GATTTCAGGGGAGCAGTATGGAATTCATTCTGATGTCTGGAGCTTAGGAATCTCTTTTATGGAGATTCAG AAAAACCAGGGATCTTTAATGCCTCTCCAGCTTCTGCAGTGCATTGTTGATGAGGATTCGCCCGTCCTTC CAGTTGGAGAGTTCTCGGAGCCATTTGTACATTTCATCACTCAGTGTATGCGAAAACAGCCAAAAGAAAG GCCAGCACCTGAAGAATTGATGGGCCACCCGTTCATCGTGCAGTTCAATGATGGAAATGCCGCCGTGGTG TCCATGTGGGTGTGCCGGGCGCTGGAGGAGAGGCGGAGCCAGCAGGGGCCCCCGTGA >OriGene 5' read for mutant NM_002757 unedited
ACCGCCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGA ACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGTCGCCGCCGCCGC AGCCGCCGCCGGTCCGCGCGGCCTCGGGTGGCCGGAGCTCAGCCTGCGCGCGCCGCGCCCTGTGTCTCCG GGTGGGGCAGAAGACTCGCCCCTTGAACCTCCCGCGGGGACTCTCCGTGGTGTGGCGGCCCTGGGGCTCT TTCTTAATAGCCCCGGACTGAGTCCCCCTCCAGTCGAGGACCCTCTCCCTAGTCCCACTGACGAACGGGT GTGGACAACCTGTGCCCGCTGTGTATTCTCCCCCCAACCCGAGTTCCTTGCCCTGTGCTTGCCCTCCCTA TATACCCCACCCCGGGCGGGCAGAGAACTTTTCACCCATACCCGTTTGCGCTTCACCCTCCCGAAACTTT TCGGAACCCTCCCGCTAGTTTCCCTGCCGGGCCCTTTTGCCCCGTTTTCCCGGGGGCCCCCCTCCCCGGG GAGAAACACCCTAGAACCCCCGACAAGACCTGGGGACGGGCCGGGGGCCCCGGGGGGGTCCTTTCCTGAA TCACCCCTTCCCTCTTTTCCTTTCCCCCTTAATCCCCTTTCCCTGGTTTTTTCCCCCTTTTGCCCTGGGG CCGGCCCCCCCCCTTTTGCCCCCTTTGGGGACCCCCTCTTAAAACCGGGGGCCAGGGGGGGTTTTCCCTA TCCCCCCCGAAGTTGGGCCCCCCTTTAAACCGTGATGGGGCTGGCGGAGCCCCTTGGTGCCCTCTTTTTC CCCCCCGGGAGACAACACATGGTGGGGTGGTTATCTCTCCACGAATACACACAATAATTGTGCGCCGCGG AGCAGATCAGAGTCAGATCACGCGGGCGCACAATATCTTCTCTGAGAGTGCTGCTGATGTGTATTGACCA CGATCTTTGCGTGCTACACACCATCACCATTGTATGTATGGATGAGAACGAGATGGCGCTACCTAGTCCG TAAGGCGATCTGCATGATT >SC323639 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
GMGATGATGACAAGACTACGATATCGGGACACTCTTGGTCATGGCAACGGAGGCACAGTCTACAAAGCAT ATCATGTCCCGAGTGGGAAAATATTAGCTGTAATGGTCATACTACTAGATATTACACTGGAACTTCAGAA GCAAATTATGTCTGAATTGGAAATTCTTTATAAGTGCGATTCATCATATATCATTGGATTTTATGGAGCA TTTTTTGTAGAAAACAGGATTTCAATATGTACAGAATTCATGGAT |
| Restriction Sites | Please inquire |
| ACCN | NM_002757 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002757.2, NP_002748.1 |
| RefSeq Size | 2334 bp |
| RefSeq ORF | 1317 bp |
| Locus ID | 5607 |
| UniProt ID | Q13163 |
| Domains | PB1, pkinase, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Gap junction, MAPK signaling pathway, Neurotrophin signaling pathway |
| Gene Summary | The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically interacts with and activates MAPK7/ERK5. This kinase itself can be phosphorylated and activated by MAP3K3/MEKK3, as well as by atypical protein kinase C isoforms (aPKCs). The signal cascade mediated by this kinase is involved in growth factor stimulated cell proliferation and muscle cell differentiation. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been described. [provided by RefSeq, May 2011] Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (B) has the same N- and C-termini but is shorter compared to isoform A. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217115 | MAP2K5 (Myc-DDK-tagged)-Human mitogen-activated protein kinase kinase 5 (MAP2K5), transcript variant 2 |
CNY 3,656.00 |
|
| RC217115L3 | Lenti ORF clone of Human mitogen-activated protein kinase kinase 5 (MAP2K5), transcript variant 2, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC217115L4 | Lenti ORF clone of Human mitogen-activated protein kinase kinase 5 (MAP2K5), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG217115 | MAP2K5 (tGFP-tagged) - Human mitogen-activated protein kinase kinase 5 (MAP2K5), transcript variant 2 |
CNY 4,370.00 |
|
| SC310389 | MAP2K5 (untagged)-Human mitogen-activated protein kinase kinase 5 (MAP2K5), transcript variant 2 |
CNY 7,030.00 |
|
| SC323569 | MAP2K5 (untagged)-Kinase deficient mutant (K195M) of Human mitogen-activated protein kinase kinase 5 (MAP2K5), transcript variant B |
CNY 3,656.00 |
