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PRPF4B (NM_003913) Human Untagged Clone
CAT＃: SC323624
PRPF4B (untagged)-Kinase deficient mutant (K717M) of Human PRP4 pre-mRNA processing factor 4 homolog B (yeast) (PRPF4B)
CN¥ 7,016.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | dJ1013A10.1; PR4H; PRP4; PRP4H; PRP4K |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323624 sequence for NM_003913 edited (data generated by NextGen Sequencing)
ATGGCCGCCGCGGAGACCCAGTCGCTACGGGAGCAGCCAGAGATGGAAGATGCTAATTCT GAAAAGAGTATAAATGAAGAAAATGGAGAAGTATCAGAAGACCAGTCTCAAAATAAGCAC AGTCGTCACAAAAAAAAGAAGCATAAACACAGAAGTAAACATAAGAAACATAAACATTCC TCAGAAGAAGACAAGGATAAAAAACATAAACATAAGCATAAACATAAGAAACACAAAAGA AAAGAGATTATTGATGCTTCTGATAAAGAGGGTATGTCTCCAGCAAAAAGAACTAAACTT GATGATTTAGCTTTGCTAGAAGACTTGGAAAAACAGAGAGCCTTGATTAAGGCCGAACTT GATAATGAGTTAATGGAAGGAAAGGTCCAGTCTGGTATGGGGCTCATTTTGCAAGGTTAT GAGTCTGGCTCTGAAGAAGAGGGGGAAATTCATGAAAAGGCAAGAAATGGAAATAGGTCT AGTACTAGATCTTCAAGTACAAAGGGGAAACTTGAACTTGTGGACAATAAAATTACTACA AAGAAACGAAGTAAAAGCAGATCCAAAGAACGGACTAGACATAGGTCTGATAAAAAGAAA AGTAAGGGGGGTATTGAAATCGTTAAAGAGAAAACAACTAGGAGCAAGTCAAAGGAGAGG AAAAAATCTAAAAGCCCATCCAAAAGAAGTAAGTCTCAAGATCAAGCAAGGAAATCAAAA TCCCCTACCCTTAGAAGGCGATCTCAAGAGAAAATTGGTAAGGCCAGATCTCCTACTGAT GATAAGGTTAAAATTGAAGATAAAAGTAAATCAAAAGATAGGAAAAAATCCCCAATTATA AATGAAAGTAGAAGTCGCGATCGAGGTAAAAAATCCAGATCCCCAGTTGATTTAAGAGGT AAATCCAAAGACAGAAGGTCACGGTCCAAAGAGAGAAAATCAAAACGGTCTGAAACTGAT AAAGAAAAGAAGCCAATTAAATCTCCCTCTAAAGATGCTTCATCTGGGAAAGAAAATAGG TCACCCAGCAGAAGACCTGGTCGTAGTCCTAAAAGAAGAAGTTTGTCTCCAAAACCACGT GATAAATCAAGAAGAAGCAGGTCTCCACTTTTGAATGATAGAAGATCTAAGCAGAGCAAA TCCCCCTCGCGGACACTGTCTCCTGGGAGAAGAGCCAAGAGCCGATCCTTAGAAAGAAAA CGACGAGAACCAGAGAGGAGACGACTTTCTTCTCCAAGAACACGACCTCGAGATGATATC CTCAGTAGACGTGAAAGATCAAAAGATGCCAGCCCCATCAATAGATGGTCTCCAACCCGA AGAAGAAGTAGATCTCCCATTAGAAGGAGGTCTCGTTCCCCACTCAGACGTAGCAGGTCT CCAAGAAGAAGAAGCAGATCTCCTCGGAGAAGGGACAGAGGTCGGAGGAGCAGATCACGC TTGCGAAGGCGGTCTCGATCACGCGGTGGTCGTAGACGAAGGAGCAGAAGCAAAGTAAAG GAAGATAAATTTAAAGGAAGTCTTTCTGAAGGAATGAAAGTTGAGCAGGAATCTTCGTCT GATGATAACCTTGAAGACTTTGATGTAGAGGAAGAAGATGAAGAAGCCCTAATAGAACAG AGAAGAATCCAAAGGCAGGCAATTGTTCAGAAATATAAATACCTTGCTGAAGATAGCAAC ATGTCTGTGCCATCTGAACCAAGCAGCCCCCAGAGCAGTACGAGAACACGATCACCATCT CCAGATGACATTCTGGAGCGAGTAGCTGCTGATGTTAAAGAGTATGAACGGGAAAATGTT GATACATTTGAGGCCTCAGTGAAAGCCAAGCATAATCTAATGACAGTTGAACAGAATAAT GGTTCATCTCAGAAGAAGTTGTTGGCACCTGATATGTTTACAGAATCTGATGATATGTTT GCTGCGTATTTTGATAGTGCTCGTCTTCGGGCCGCTGGCATTGGAAAAGATTTCAAAGAG AATCCCAACCTCAGAGATAACTGGACCGATGCAGAAGGCTATTATCGTGTGAACATAGGT GAAGTCCTAGATAAACGTTACAATGTGTATGGCTACACTGGGCAAGGTGTATTCAGTAAT GTTGTACGAGCCAGAGATAATGCAAGAGCCAACCAAGAAGTGGCTGTAATGATCATCAGA AACAATGAGCTCATGCAAAAGACTGGTTTAAAAGAATTAGAGTTCTTGAAAAAACTTAAT GATGCTGATCCTGATGACAAATTTCATTGTCTGAGACTCTTCAGGCACTTCTATCACAAG CAGCATCTTTGTCTGGTATTCGAGCCTCTCAGCATGAACTTACGAGAGGTGTTAAAAAAA TATGGTAAAGATGTTGGTCTTCATATTAAAGCTGTAAGATCCTATAGTCAGCAGTTGTTC CTGGCATTGAAACTCCTTAAAAGATGCAATATCCTACATGCAGATATCAAGCCAGACAAT ATCCTGGTTAATGAATCCAAAACTATTTTAAAGCTTTGCGATTTTGGGTCGGCTTCACAT GTTGCGGATAATGACATAACACCTTATCTTGTCAGTAGATTTTATCGTGCTCCTGAAATC ATTATAGGTAAAAGCTATGACTATGGTATAGATATGTGGTCTGTAGGTTGCACCTTATAC GAACTCTATACTGGAAAAATTTTATTCCCTGGCAAAACCAATAACCATATGCTGAAGCTT GCAATGGATCTCAAAGGAAAGATGCCAAATAAGATGATTCGAAAAGGTGTGTTCAAAGAT CAGCATTTTGATCAAAATCTCAACTTCATGTACATAGAAGTTGATAAAGTAACAGAGAGG GAGAAAGTTACTGTTATGAGCACCATTAATCCAACTAAGGACCTGTTGGCTGACTTGATT GGGTGCCAGAGACTTCCTGAAGACCAACGTAAGAAAGTACACCAGCTAAAGGACTTGTTG GACCAGATTCTGATGTTGGACCCAGCTAAACGAATTAGCATCAACCAGGCCCTACAGCAC GCCTTCATCCAGGAAAAAATTTAA Clone variation with respect to NM_003913.4 2150 a=>t >OriGene 5' read for mutant NM_003913 unedited
CCGCCCGTTGAGCAATGGGCGGTAGGCGTGTACGGAGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCGGGAGCCGCCGC CACCGCCGCCGAGGAGTCAGGAAGTTCAAGATGGCCGCCGCGGAGACCCAGTCGCTACGGGAGCAGCCAG AGATGGAAGATGCTAATTCTGAAAAGAGTATAAATGAAGAAAATGGAGAAGTATCAGAAGACCAGTCTCA AAATAAGCACAGTCGTCACAAAAAAAAGAAGCATAAACACAGAAGTAAACATAAGAAACATAAACGTTCC TCAGAGGAGAACAAGGATAAAAAACATAAACATAAGCTTAACCTTAAGAAACCCAAAAGAAAAGGAGATT ATTGATGGCTTCCTGATAAGAAGGGTATGGTCTCCGGCAAAAAGACCTAAACTGATTGATTTACCTTGGT TGGAGAACTTGGAAAAACGGAGAGCCTGGTTAAAGGCGAACTTGTTAGGAGTTTGGGAGGGAGGGTCCGG TTGTGATGGGGGCTATTTTGCAGGTTAGACCGGTTCTGAAGAGGGGGGAATTCGGAAAGGGAAAATGAAA TGGTATATATATATCTCGATAAAGGGGACTCTGCTTTGTGCCCTAAAATTCCTCAGAACGAATTAACCGT CCCAGACGGCTTCCTTGGTGGATAAGAAGATGGGGGTTGAACGTTAGGAAACTTGGCGTCTAGGAGAAAT TAGCTCCAAATGTCCATAGCGGACATCTCTCTAGAGTCAGAATGAGCGATCTCGAAAGGATGAAGATACA TAGGATCCTAAGTAGTGCTTCGTAATCTTCTTTG >SC323624 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
GKGCWTGGCTAMCTGGGCAGGTGTATTCAGTAATGTTGTACGAGCCAGAGATAATGCAAGAGCCAACCAA GAAGTGGCTGTAATGATCATCAGAAACAATGAGCTCATGCAAAAGACTGGTTTAAAAGAATTAGAGTTCT TGAAAAAACTTAATGATGCTGATCCTGATGACAAATTTCATTGTCTGAGACTCTTCAGGCACTTCTATCA CAAGCAGCATCTTTGTCTGGTATTCGAGCCTCTCAGCATGAACTT |
| Restriction Sites | Please inquire |
| ACCN | NM_003913 |
| Insert Size | 5000 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003913.3, NP_003904.2 |
| RefSeq Size | 6681 bp |
| RefSeq ORF | 3024 bp |
| Locus ID | 8899 |
| UniProt ID | Q13523 |
| Domains | pkinase, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | Pre-mRNA splicing occurs in two sequential transesterification steps, and the protein encoded by this gene is thought to be involved in pre-mRNA splicing and in signal transduction. This protein belongs to a kinase family that includes serine/arginine-rich protein-specific kinases and cyclin-dependent kinases (CDKs). This protein is regarded as a CDK-like kinase (Clk) with homology to mitogen-activated protein kinases (MAPKs). [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) represents the protein-coding transcript. |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205508 | PRPF4B (Myc-DDK-tagged)-Human PRP4 pre-mRNA processing factor 4 homolog B (yeast) (PRPF4B) |
CN¥ 7,016.00 |
|
| RC205508L1 | Lenti ORF clone of Human PRP4 pre-mRNA processing factor 4 homolog B (yeast) (PRPF4B), Myc-DDK-tagged |
CN¥ 9,416.00 |
|
| RC205508L2 | Lenti ORF clone of Human PRP4 pre-mRNA processing factor 4 homolog B (yeast) (PRPF4B), mGFP tagged |
CN¥ 10,550.00 |
|
| RC205508L3 | Lenti ORF clone of Human PRP4 pre-mRNA processing factor 4 homolog B (yeast) (PRPF4B), Myc-DDK-tagged |
CN¥ 10,550.00 |
|
| RC205508L4 | Lenti ORF clone of Human PRP4 pre-mRNA processing factor 4 homolog B (yeast) (PRPF4B), mGFP tagged |
CN¥ 10,550.00 |
|
| RG205508 | PRPF4B (tGFP-tagged) - Human PRP4 pre-mRNA processing factor 4 homolog B (yeast) (PRPF4B) |
CN¥ 8,616.00 |
|
| SC107962 | PRPF4B (untagged)-Human PRP4 pre-mRNA processing factor 4 homolog B (yeast) (PRPF4B) |
CN¥ 7,016.00 |
