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LCK (NM_005356) Human Untagged Clone
CAT＃: SC323616
LCK (untagged)-Kinase deficient mutant (K273M) of Human lymphocyte-specific protein tyrosine kinase (LCK), transcript variant 2
CNY 3,800.00
CNY 8,270.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | IMD22; LSK; p56lck; pp58lck; YT16 |
| Vector | pCMV6-XL6 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_005356, the custom clone sequence may differ by one or more nucleotides
ATGGGCTGTGGCTGCAGCTCACACCCGGAAGATGACTGGATGGAAAACATCGATGTGTGTGAGAACTGCC ATTATCCCATAGTCCCACTGGATGGCAAGGGCACGCTGCTCATCCGAAATGGCTCTGAGGTGCGGGACCC ACTGGTTACCTACGAAGGCTCCAATCCGCCGGCTTCCCCACTGCAAGACAACCTGGTTATCGCTCTGCAC AGCTATGAGCCCTCTCACGACGGAGATCTGGGCTTTGAGAAGGGGGAACAGCTCCGCATCCTGGAGCAGA GCGGCGAGTGGTGGAAGGCGCAGTCCCTGACCACGGGCCAGGAAGGCTTCATCCCCTTCAATTTTGTGGC CAAAGCGAACAGCCTGGAGCCCGAACCCTGGTTCTTCAAGAACCTGAGCCGCAAGGACGCGGAGCGGCAG CTCCTGGCGCCCGGGAACACTCACGGCTCCTTCCTCATCCGGGAGAGCGAGAGCACCGCGGGATCGTTTT CACTGTCGGTCCGGGACTTCGACCAGAACCAGGGAGAGGTGGTGAAACATTACAAGATCCGTAATCTGGA CAACGGTGGCTTCTACATCTCCCCTCGAATCACTTTTCCCGGCCTGCATGAACTGGTCCGCCATTACACC AATGCTTCAGATGGGCTGTGCACACGGTTGAGCCGCCCCTGCCAGACCCAGAAGCCCCAGAAGCCGTGGT GGGAGGACGAGTGGGAGGTTCCCAGGGAGACGCTGAAGCTGGTGGAGCGGCTGGGGGCTGGACAGTTCGG GGAGGTGTGGATGGGGTACTACAACGGGCACACGAAGGTGGCGGTGAAGAGCCTGAAGCAGGGCAGCATG TCCCCGGACGCCTTCCTGGCCGAGGCCAACCTCATGAAGCAGCTGCAACACCAGCGGCTGGTTCGGCTCT ACGCTGTGGTCACCCAGGAGCCCATCTACATCATCACTGAATACATGGAGAATGGGAGTCTAGTGGATTT TCTCAAGACCCCTTCAGGCATCAAGTTGACCATCAACAAACTCCTGGACATGGCAGCCCAAATTGCAGAA GGCATGGCATTCATTGAAGAGCGGAATTATATTCATCGTGACCTTCGGGCTGCCAACATTCTGGTGTCTG ACACCCTGAGCTGCAAGATTGCAGACTTTGGCCTAGCACGCCTCATTGAGGACAACGAGTACACAGCCAG GGAGGGGGCCAAGTTTCCCATTAAGTGGACAGCGCCAGAAGCCATTAACTACGGGACATTCACCATCAAG TCAGATGTGTGGTCTTTTGGGATCCTGCTGACGGAAATTGTCACCCACGGCCGCATCCCTTACCCAGGGA TGACCAACCCGGAGGTGATTCAGAACCTGGAGCGAGGCTACCGCATGGTGCGCCCTGACAACTGTCCAGA GGAGCTGTACCAACTCATGAGGCTGTGCTGGAAGGAGCGCCCAGAGGACCGGCCCACCTTTGACTACCTG CGCAGTGTGCTGGAGGACTTCTTCACGGCCACAGAGGGCCAGTACCAGCCTCAGCCTTGA >OriGene 5' read for mutant NM_005356 unedited
CCCGCCCGTCCAGCACTGGGCGGTAGGCGCTGTACGGCTGGGAGGTTCTATATAAGCAGAGCTCGTTTAG TGAACCGTCAGAATCTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGACGGCGAA GGGAGCTGAGACTGTCCAGGCAGCCAGGCTAGGCCAGGAGGACCATGTGAATGGGGCCAGAGGGCTCCCG GGCTGGGCAGGGACCATGGGCTGTGGCTGCAGCTCACACCCGGAAGATGACTGGATGGAAAACATCGATG TGTGTGAGAACTGCCATTATCCCATAGTCCCACTGGATGGCAAGGCACGCTGCTCATCCGAAATGGCTCT GAGGTGCGGGGACCCACTTGGTTACCTACCGGAAGGCCTCCCATTTCCGCCCGGCTTTCCCCACTGTGCA AGGGTGACCCCCAGGCCAGCAGGGGCCTGGAAAAACCAAGGCCCTGCCGGATCCCTGGCCTGGTTGCCCT CCCCCCCTCTTCCCCCACCCTACTTTTTTCCCCGTTCCTGGCTTTTCTTGTGCCCCCCCCCCCTGGTACC TCCAAGGCTTCTGCCCCGATCCCAGTCTGGTTTCCCCCGGTACCCCCTTTTGTTTTTCCAAAAAACCTGG TTTTATCTCTGCACAGCGTTGAAACCCCCTCCCCACAAGAAAATTGGGGGTTTAGAAAAGGGGGAAAAGC CCGCATTCCCCGGGACGCGAATTTTTTTTTTAAATTTTGTTTGGGGAAACCCCGGGGGGGGAGCGGATTT TCCCGACCCCAACCCCCCTGGGGGGGCCTTTATCAACCTTTGGGGGGGCCCCCCTTTTGACAAAAATTTT AGGCTCCCTTATTTTTCTGAACCCCGGGTTGGGGGGAGCTGGGTTATAGGGGGGTAGGAGGTTTTTTAGA AGGAGGTCTATACTACTCACTTATCACCCACTCTCCCCACCCTCGTGGTGTCAGAAGGCGATTGGTGGAG AGCCCTATTTCCCGAGCCCGAAGGTATGCCTTTTATGTTGTGCACAGAGACAATCCGTGTACAGCGACGC TCTTTTATACAAACGTATCAT >SC323616 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
GATGCWGCGCCTGRTACTGKGRCGGCTGGGGCTGGMAGTTCGGGGAGGTGTGGATGGGGTACTACAACGG GCACACGAAGGTGGCGGTGATGAGCCTGAAGCAGGGCAGCATGTCCCCGGACGCCTTCCTGGCCGAGGCC AACCTCATGAAGCAGCTGCAACACCAGCGGCTGGTTCGGCTCTACGCTGKGGTCACCCAGGAGCCCATCT ACATCATCACTGAATACATGGARAATGGGAGTCTAGTGGATTTTC |
| Restriction Sites | Please inquire |
| ACCN | NM_005356 |
| Insert Size | 2740 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005356.2, NP_005347.2 |
| RefSeq Size | 2032 bp |
| RefSeq ORF | 1530 bp |
| Locus ID | 3932 |
| UniProt ID | P06239 |
| Domains | pkinase, SH2, TyrKc, SH3, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase, Stem cell - Pluripotency |
| Protein Pathways | Natural killer cell mediated cytotoxicity, Primary immunodeficiency, T cell receptor signaling pathway |
| Gene Summary | This gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein is a key signaling molecule in the selection and maturation of developing T-cells. It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to the plasma membrane and pericentrosomal vesicles, and binds to cell surface receptors, including CD4 and CD8, and other signaling molecules. Multiple alternatively spliced variants encoding different isoforms have been described. [provided by RefSeq, Aug 2016] Transcript Variant: This variant (2) is transcribed from the distal type II promoter and contains a distinct 5' UTR compared to variant 1. Variants 1 and 2 encode the same isoform (a). |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC219375 | LCK (Myc-DDK-tagged)-Human lymphocyte-specific protein tyrosine kinase (LCK), transcript variant 2 |
CNY 5,688.00 |
|
| RC219375L1 | Lenti ORF clone of Human lymphocyte-specific protein tyrosine kinase (LCK), transcript variant 2, Myc-DDK-tagged |
CNY 8,088.00 |
|
| RC219375L2 | Lenti ORF clone of Human lymphocyte-specific protein tyrosine kinase (LCK), transcript variant 2, mGFP tagged |
CNY 6,370.00 |
|
| RC219375L3 | Lenti ORF clone of Human lymphocyte-specific protein tyrosine kinase (LCK), transcript variant 2, Myc-DDK-tagged |
CNY 6,370.00 |
|
| RC219375L4 | Lenti ORF clone of Human lymphocyte-specific protein tyrosine kinase (LCK), transcript variant 2, mGFP tagged |
CNY 8,088.00 |
|
| RG219375 | LCK (tGFP-tagged) - Human lymphocyte-specific protein tyrosine kinase (LCK), transcript variant 2 |
CNY 7,288.00 |
|
| SC116770 | LCK (untagged)-Human lymphocyte-specific protein tyrosine kinase (LCK), transcript variant 2 |
CNY 3,800.00 |
