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CSNK1G1 (NM_022048) Human Untagged Clone
CAT＃: SC323608
CSNK1G1 (untagged)-Kinase deficient mutant (K73M) of Human casein kinase 1, gamma 1 (CSNK1G1)
CNY 3,656.00
CNY 7,220.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CK1gamma1 |
| Vector | pCMV6-XL6 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323608 sequence for NM_022048 edited (data generated by NextGen Sequencing)
ATGGACCATCCTAGTAGGGAAAAGGATGAAAGACAACGGACAACTAAACCCATGGCACAA AGGAGTGCACACTGCTCTCGACCATCTGGCTCCTCATCGTCCTCTGGGGTTCTTATGGTG GGACCCAACTTCAGGGTTGGCAAGAAGATAGGATGTGGGAACTTCGGAGAGCTCAGATTA GGTAAAAATCTCTACACCAATGAATATGTAGCAATCAAACTGGAACCAATAAAATCACGT GCTCCACAGCTTCATTTAGAGTACAGATTTTATAAACAGCTTGGCAGTGCAGGTGAAGGT CTCCCACAGGTGTATTACTTTGGACCATGTGGGAAATATAATGCCATGGTGCTGGAGCTC CTTGGCCCTAGCTTGGAGGACTTGTTTGACCTCTGTGACCGAACATTTACTTTGAAGACG GTGTTAATGATAGCCATCCAGCTGCTTTCTCGAATGGAATACGTGCACTCAAAGAACCTC ATTTACCGAGATGTCAAGCCAGAGAACTTCCTGATTGGTCGACAAGGCAATAAGAAAGAG CATGTTATACACATTATAGACTTTGGACTGGCCAAGGAATACATTGACCCCGAAACCAAA AAACACATACCTTATAGGGAACACAAAAGTTTAACTGGAACTGCAAGATATATGTCTATC AACACGCATCTTGGCAAAGAGCAAAGCCGGAGAGATGATTTGGAAGCCCTAGGCCATATG TTCATGTATTTCCTTCGAGGCAGCCTCCCCTGGCAAGGACTCAAGGCTGACACATTAAAA GAGAGATATCAAAAAATTGGTGACACCAAAAGGAATACTCCCATTGAAGCTCTCTGTGAG AACTTTCCAGAGGAGATGGCAACCTACCTTCGATATGTCAGGCGACTGGACTTCTTTGAA AAACCTGATTATGAGTATTTACGGACCCTCTTCACAGACCTCTTTGAAAAGAAAGGCTAC ACCTTTGACTATGCCTATGATTGGGTTGGGAGACCTATTCCTACTCCAGTAGGGTCAGTT CACGTAGATTCTGGTGCATCTGCAATAACTCGAGAAAGCCACACACATAGGGATCGGCCA TCACAACAGCAGCCTCTTCGAAATCAGGTGGTTAGCTCAACCAATGGAGAGCTGAATGTT GATGATCCCACGGGAGCCCACTCCAATGCACCAATCACAGCTCATGCCGAGGTGGAGGTA GTGGAGGAAGCTAAGTGCTGCTGTTTCTTTAAGAGGAAAAGGAAGAAGACTGCTCAGCGC CACAAGTGA Clone variation with respect to NM_022048.3 >OriGene 5' read for mutant NM_022048 unedited
CCCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGGTTCACCTAAGATTG AGACCTAGTGACTACATTTCCTACGGGAACAAATAAATGGTTTTTCATCTCCCGGAGATACATTACAAAC AAATATGGTGCTAAAAGAACTCCTTACCTTTCTCTGACTACAATTTATTTGGACATACTTTTGTATTGAA GAGAGGTATACATACTGAAGCTACTTGCTGTACTATAGGAGACTCTGTCCTGTAGGATCATGGACCATCC TAGTAGGGAAAAGGATGAAAGACAACGGACAACTAAAACCCATGGCAACAAAGGAGTGCACACTGCTCTC GACCATCTGGCTCCTCATCGTCCCTCTGGGGTTCCTTATGGGTGGGACCCCACTTTCAGGGTTGGCAAAG AAGATAGGATGGTGGGACCTCCGGAAGAGCTAGATAGGTAAAAATCTCTCCCCAATGAATTTGTAGCATT CATGCCTGAAACAATAAATCCCGTTCTCCCCGGCTTCATTAAAATCCGATTTATAAAACGCTGGGCAGTG CAGTGAAGGTTTCCAAAGTTGATTTCCTTTGACCCTGGGGGAAATAAATGCCTGGGTCGTGGACTCTTTG GCCCTCACTGAGAGCTTTGTTGACCTGTGACCGCACTTCTTTGAACCGGTATAGTACGCACCTCTCTCCC AGGGAACTGACTCAAACTAATACGAGTAGAGCGACTCGAGTGCCAGCATGAGACGTTAACTTAGCTGAAC GCGAACCTGGCGGACAGACATTAGAACAGATCAGATCGAGTCTCAC >SC323608 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
CTCGMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCA GAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGGTTCACCTAAGATTGAGACCT AGTGACTACATTTCCTACGGGAACAAATAAATGGTTTTTCATCTCCCGGAGATACATTACAAACAAATAT GGTGCTAAAAGAACTCCTTACCTTTCTCTGACTACAATTTATTTG |
| Restriction Sites | Please inquire |
| ACCN | NM_022048 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_022048.3, NP_071331.2 |
| RefSeq Size | 8163 bp |
| RefSeq ORF | 1269 bp |
| Locus ID | 53944 |
| UniProt ID | Q9HCP0 |
| Domains | pkinase, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Hedgehog signaling pathway |
| Gene Summary | This gene encodes a member of the casein kinase I gene family. This family is comprised of serine/threonine kinases that phosphorylate acidic proteins such as caseins. The encoded kinase plays a role in cell cycle checkpoint arrest in response to stalled replication forks by phosphorylating Claspin. A mutation in this gene may be associated with non-syndromic early-onset epilepsy (NSEOE). [provided by RefSeq, Jul 2016] Transcript Variant: This variant (1) represents the longest transcript and encodes the shortest isoform (1). Both variants 1 and 4 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC224794 | CSNK1G1 (Myc-DDK-tagged)-Human casein kinase 1, gamma 1 (CSNK1G1) |
CNY 3,656.00 |
|
| RC224794L1 | Lenti ORF clone of Human casein kinase 1, gamma 1 (CSNK1G1), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC224794L2 | Lenti ORF clone of Human casein kinase 1, gamma 1 (CSNK1G1), mGFP tagged |
CNY 5,890.00 |
|
| RC224794L3 | Lenti ORF clone of Human casein kinase 1, gamma 1 (CSNK1G1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC224794L4 | Lenti ORF clone of Human casein kinase 1, gamma 1 (CSNK1G1), mGFP tagged |
CNY 5,890.00 |
|
| RG224794 | CSNK1G1 (tGFP-tagged) - Human casein kinase 1, gamma 1 (CSNK1G1) |
CNY 5,256.00 |
|
| SC311378 | CSNK1G1 (untagged)-Human casein kinase 1, gamma 1 (CSNK1G1) |
CNY 7,220.00 |
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