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YES1 (NM_005433) Human Untagged Clone
CAT＃: SC323601
YES1 (untagged)-Kinase deficient mutant (K305M) of Human v-yes-1 Yamaguchi sarcoma viral oncogene homolog 1 (YES1)
CNY 4,048.00
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | c-yes; HsT441; P61-YES; Yes |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_005433, the custom clone sequence may differ by one or more nucleotides
ATGGGCTGCATTAAAAGTAAAGAAAACAAAAGTCCAGCCATTAAATACAGACCTGAAAATACTCCAGAGC CTGTCAGTACAAGTGTGAGCCATTATGGAGCAGAACCCACTACAGTGTCACCATGTCCGTCATCTTCAGC AAAGGGAACAGCAGTTAATTTCAGCAGTCTTTCCATGACACCATTTGGAGGATCCTCAGGGGTAACGCCT TTTGGAGGTGCATCTTCCTCATTTTCAGTGGTGCCAAGTTCATATCCTGCTGGTTTAACAGGTGGTGTTA CTATATTTGTGGCCTTATATGATTATGAAGCTAGAACTACAGAAGACCTTTCATTTAAGAAGGGTGAAAG ATTTCAAATAATTAACAATACGGAAGGAGATTGGTGGGAAGCAAGATCAATCGCTACAGGAAAGAATGGT TATATCCCGAGCAATTATGTAGCGCCTGCAGATTCCATTCAGGCAGAAGAATGGTATTTTGGCAAAATGG GGAGAAAAGATGCTGAAAGATTACTTTTGAATCCTGGAAATCAACGAGGTATTTTCTTAGTAAGAGAGAG TGAAACAACTAAAGGTGCTTATTCCCTTTCTATTCGTGATTGGGATGAGATAAGGGGTGACAATGTGAAA CACTACAAAATTAGGAAACTTGACAATGGTGGATACTATATCACAACCAGAGCACAATTTGATACTCTGC AGAAATTGGTGAAACACTACACAGAACATGCTGATGGTTTATGCCACAAGTTGACAACTGTGTGTCCAAC TGTGAAACCTCAGACTCAAGGTCTAGCAAAAGATGCTTGGGAAATCCCTCGAGAATCTTTGCGACTAGAG GTTAAACTAGGACAAGGATGTTTCGGCGAAGTGTGGATGGGAACATGGAATGGAACCACGAAAGTAGCAA TCAAAACACTAAAACCAGGTACAATGATGCCAGAAGCTTTCCTTCAAGAAGCTCAGATAATGAAAAAATT AAGACATGATAAACTTGTTCCACTATATGCTGTTGTTTCTGAAGAACCAATTTACATTGTCACTGAATTT ATGTCAAAAGGAAGCTTATTAGATTTCCTTAAGGAAGGAGATGGAAAGTATTTGAAGCTTCCACAGCTGG TTGATATGGCTGCTCAGATTGCTGATGGTATGGCATATATTGAAAGAATGAACTATATTCACCGAGATCT TCGGGCTGCTAATATTCTTGTAGGAGAAAATCTTGTGTGCAAAATAGCAGACTTTGGTTTAGCAAGGTTA ATTGAAGACAATGAATACACAGCAAGACAAGGTGCAAAATTTCCAATCAAATGGACAGCTCCTGAAGCTG CACTGTATGGTCGGTTTACAATAAAGTCTGATGTCTGGTCATTTGGAATTCTGCAAACAGAACTAGTAAC AAAGGGCCGAGTGCCATATCCAGGTATGGTGAACCGTGAAGTACTAGAACAAGTGGAGCGAGGATACAGG ATGCCGTGCCCTCAGGGCTGTCCAGAATCCCTCCATGAATTGATGAATCTGTGTTGGAAGAAGGACCCTG ATGAAAGACCAACATTTGAATATATTCAGTCCTTCTTGGAAGACTACTTCACTGCTACAGAGCCACAGTA CCAGCCAGGAGAAAATTTATAA >OriGene 5' read for mutant NM_005433 unedited
CCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAAC CGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGAAGTGACACGGACC GGTCGCCTATCCTGACCACAGCAAAGCGGCCCGGAGCCCGCGGAGGGGACCTGACGGGGGCGTAGGCGCC GGAAGGCTGGGGGCCCCGGAGCCGGGCCGGCGCGGCCCGAGTTCCGGTGAGCGGACGGCGGCGCGCGCAG ATTTGATAATGGGCTGCATTAAAAGTAAAGAAAACAAAAGTCCAGCCATTAAATACAGACCTGAAAATAC TCCCAGAGCCCTGTCAGTACAAGTGTGAGCCCATTATGGGAGCAGACCCCACTACAGTTGTCCACCATGT CCCGTCATCCTTCAGCAAAGGGGAACAGCAGTTTAATTTTCAGCAGTTCTTTCCCATGACCACCATTTTG GGAGGATCCTTCAGGGGTACCGCCCTTTTGGGAGGGTGCATTCTTCCTCATTTTTCAGGTGGGTGCAAGT TTCTATATCCTCGTGGTTTAACCAGGTGGTGTACCATATTTTTGGCCCTAAATGATATATGAGCTAGAAC CTACGAGAGACTTTTCTTTAGAAGGGTAAAGATTCCATATATTAACATCCGGAAGGAGATGGGGAACCGA TCTACTCGCTAGGAAAATGGTATTCCAGACATTGTGACGCTCGCAATCCTTCGGCAAATGTTTGGCCAAT GGAAAATGTCGAAATCTTGAATCCGGATCCAGGAATTCTGATAGAAAAGTGAAACTAAAGCCCTATCCTA TACCCAGTGTGAATATGGGTTTACGTGAGAAAACACAATATGAGAT >SC323601 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
CTGCATCCTCGAGATCTTTGCGACTAGAGGTTAAACTAGGACAAGGATGTTTCGGCGAAGTGTGGATGGG AACATGGAATGGAACCACGAAAGTAGCAATCATGACACTAAAACCAGGTACAATGATGCCAGAAGCTTTC CTTCAAGAAGCTCAGATAATGAAAAAATTAAGACATGATAAACTTGTTCCACTATATGCTGTTGTTTCTG AAGAACCAATTTACATTGTCACTGAATTTATGTCAAAAGGAAGCT |
| Restriction Sites | Please inquire |
| ACCN | NM_005433 |
| Insert Size | 4230 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005433.3, NP_005424.1 |
| RefSeq Size | 4685 bp |
| RefSeq ORF | 1632 bp |
| Locus ID | 7525 |
| UniProt ID | P07947 |
| Domains | pkinase, SH2, TyrKc, SH3, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Adherens junction, Tight junction |
| Gene Summary | This gene is the cellular homolog of the Yamaguchi sarcoma virus oncogene. The encoded protein has tyrosine kinase activity and belongs to the src family of proteins. This gene lies in close proximity to thymidylate synthase gene on chromosome 18, and a corresponding pseudogene has been found on chromosome 22. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208432 | YES1 (Myc-DDK-tagged)-Human v-yes-1 Yamaguchi sarcoma viral oncogene homolog 1 (YES1) |
CNY 4,040.00 |
|
| RC208432L1 | Lenti ORF clone of Human v-yes-1 Yamaguchi sarcoma viral oncogene homolog 1 (YES1), Myc-DDK-tagged |
CNY 6,440.00 |
|
| RC208432L2 | Lenti ORF clone of Human v-yes-1 Yamaguchi sarcoma viral oncogene homolog 1 (YES1), mGFP tagged |
CNY 5,890.00 |
|
| RC208432L3 | Lenti ORF clone of Human v-yes-1 Yamaguchi sarcoma viral oncogene homolog 1 (YES1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC208432L4 | Lenti ORF clone of Human v-yes-1 Yamaguchi sarcoma viral oncogene homolog 1 (YES1), mGFP tagged |
CNY 6,440.00 |
|
| RG208432 | YES1 (tGFP-tagged) - Human v-yes-1 Yamaguchi sarcoma viral oncogene homolog 1 (YES1) |
CNY 5,640.00 |
|
| SC116734 | YES1 (untagged)-Human v-yes-1 Yamaguchi sarcoma viral oncogene homolog 1 (YES1) |
CNY 4,048.00 |
