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LIM kinase 2 (LIMK2) (NM_005569) Human Untagged Clone
CAT＃: SC323590
LIMK2 (untagged)-Kinase deficient mutant (K360M) of Human LIM domain kinase 2 (LIMK2), transcript variant 2a
CNY 4,760.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_005569, the custom clone sequence may differ by one or more nucleotides
ATGTCCGCGCTGGCGGGTGAAGATGTCTGGAGGTGTCCAGGCTGTGGGGACCACATTGCTCCAAGCCAGA TATGGTACAGGACTGTCAACGAAACCTGGCACGGCTCTTGCTTCCGGTGTTCAGAATGCCAGGATTCCCT CACCAACTGGTACTATGAGAAGGATGGGAAGCTCTACTGCCCCAAGGACTACTGGGGGAAGTTTGGGGAG TTCTGTCATGGGTGCTCCCTGCTGATGACAGGGCCTTTTATGGTGGCTGGGGAGTTCAAGTACCACCCAG AGTGCTTTGCCTGTATGAGCTGCAAGGTGATCATTGAGGATGGGGATGCATATGCACTGGTGCAGCATGC CACCCTCTACTGTGGGAAGTGCCACAATGAGGTGGTGCTGGCACCCATGTTTGAGAGACTCTCCACAGAG TCTGTTCAGGAGCAGCTGCCCTACTCTGTCACGCTCATCTCCATGCCGGCCACCACTGAAGGCAGGCGGG GCTTCTCCGTGTCCGTGGAGAGTGCCTGCTCCAACTACGCCACCACTGTGCAAGTGAAAGAGGTCAACCG GATGCACATCAGTCCCAACAATCGAAACGCCATCCACCCTGGGGACCGCATCCTGGAGATCAATGGGACC CCCGTCCGCACACTTCGAGTGGAGGAGGTGGAGGATGCAATTAGCCAGACGAGCCAGACACTTCAGCTGT TGATTGAACATGACCCCGTCTCCCAACGCCTGGACCAGCTGCGGCTGGAGGCCCGGCTCGCTCCTCACAT GCAGAATGCCGGACACCCCCACGCCCTCAGCACCCTGGACACCAAGGAGAATCTGGAGGGGACACTGAGG AGACGTTCCCTAAGGCGCAGTAACAGTATCTCCAAGTCCCCTGGCCCCAGCTCCCCAAAGGAGCCCCTGC TGTTCAGCCGTGACATCAGCCGCTCAGAATCCCTTCGTTGTTCCAGCAGCTATTCACAGCAGATCTTCCG GCCCTGTGACCTAATCCATGGGGAGGTCCTGGGGAAGGGCTTCTTTGGGCAGGCTATCAAGGTGACACAC AAAGCCACGGGCAAAGTGATGGTCATGAAAGAGTTAATTCGATGTGATGAGGAGACCCAGAAAACTTTTC TGACTGAGGTGAAAGTGATGCGCAGCCTGGACCACCCCAATGTGCTCAAGTTCATTGGTGTGCTGTACAA GGATAAGAAGCTGAACCTCCTGACAGAGTACATTGAGGGGGGCACACTGAAGGACTTTCTGCGCAGTATG GATCCGTTCCCCTGGCAGCAGAAGGTCAGGTTTGCCAAAGGAATCGCCTCCGGAATGGCCTATTTGCACT CTATGTGCATCATCCACCGGGATCTGAACTCGCACAACTGCCTCATCAAGTTGGACAAGACTGTGGTGGT GGCAGACTTTGGGCTGTCACGGCTCATAGTGGAAGAGAGGAAAAGGGCCCCCATGGAGAAGGCCACCACC AAGAAACGCACCTTGCGCAAGAACGACCGCAAGAAGCGCTACACGGTGGTGGGAAACCCCTACTGGATGG CCCCTGAGATGCTGAACGGAAAGAGCTATGATGAGACGGTGGATATCTTCTCCTTTGGGATCGTTCTCTG TGAGATCATTGGGCAGGTGTATGCAGATCCTGACTGCCTTCCCCGAACACTGGACTTTGGCCTCAACGTG AAGCTTTTCTGGGAGAAGTTTGTTCCCACAGATTGTCCCCCGGCCTTCTTCCCGCTGGCCGCCATCTGCT GCAGACTGGAGCCTGAGAGCAGACCAGCATTCTCGAAATTGGAGGACTCCTTTGAGGCCCTCTCCCTGTA CCTGGGGGAGCTGGGCATCCCGCTGCCTGCAGAGCTGGAGGAGTTGGACCACACTGTGAGCATGCAGTAC GGCCTGACCCGGGACTCACCTCCCTAG >OriGene 5' read for mutant NM_005569 unedited
ACCGCCCGTATCAGCAATGGGCGGTAGGCGCTGTACGGATGGGAGGTCTATATAAGCAGAGCTCGTTTAG TGAACCGTCAGAATCTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGAACTAAGGG GAGCTGCTGTGTCCCCCGCCTCCTCCTCCCCATTTCCGCGCTCCCGGGACCATGTCCGCGCTGGCGGGTG AAGATGTCTGGAGGTGTCCAGGCTGTGGGGACCACATTGCTCCAAGCCAGATATGGTACAGGACTGTCAA CGAAACCTGGCACGGCTCTTGCTTCCGGTGTTCAGAATGCCAGGATTCCCTCACCAACTGGTACTATGAG AAGATGGGAAGGCTTCTTACTTGCCCCCAAGGACACTACCTGGGGGAAGAGTTTTGGGGATTCTCGTTCA TGGTTGCTCCCTGGCTGATGAACAAGGCCCCTTTTAATGGGGCCTGGGGGAGTTCAATTCCCACCCCAAA TTGCTTTTGCTTGGTTTAACCCTGCAGGGGGATTCTTTGGGAATGGGGAAAGCCATTTCCCTGGGTCCCC CTGTCCCCCCCCTCAATTGGGGGAAATGTGCCAAAGGGGGGGGGGGCCGGGCCCCCATTTTTTGAAAAAA CTCCCCAAAAAATTTTTTTCAGGAACCTGTCCCCCTATTTTTGCCCCCTTATTCTTGTCGGGGCCCACCA TAAAAGGGGGGGGGGGGTTTTTCCCGTTTTCCGGGGAAAGAGGTGTTTTTAAAATAACGACAATTGGGGC AATAAAAAAAAGCACCCCGGGAGTCCCCGATCCCCCAAAATAAAAAACCCCCCCCCCCCTGGGGCACCCT CCTCCGGAAATTATGGGGGGCACTTCTTCGCTTTTTTTGTGTGGAGGGTGGGGGAAGACCTTTTCACCCA AAGCCCAAATATTCTCTGTTGTGAGAATAATAGCACCTCTCCCAACCCGTAGCAACATGGCATCGTAAGC GCGCGGTTCTCTATGTCTGTAATAATGACTACCACTCCACCCTCTTCTCTCGCGTGCACGACGAGAATAT >SC323590 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
TKAYTTCTGCTGTGACTATCCATGGGGAGGTCCTGGGGAAGGGCTTCTTTGGGCAGGCTATCAAGGTGAC ACACAAAGCCACGGGCAAAGTGATGGTCATGATGGAGTTAATTCGATGTGATGAGGAGACCCAGAAAACT TTTCTGACTGAGGTGAAAGTGATGCGCAGCCTGGACCACCCCAATGTGCTCAAGTTCATTGGTGTGCTGT ACAAGGATAAGAAGCTGAACCTGCTGACAGAGTACATTGAGGGGG |
| Restriction Sites | Please inquire |
| ACCN | NM_005569 |
| Insert Size | 4690 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005569.3, NP_005560.1 |
| RefSeq Size | 3701 bp |
| RefSeq ORF | 1917 bp |
| Locus ID | 3985 |
| UniProt ID | P53671 |
| Domains | pkinase, TyrKc, PDZ, LIM, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Axon guidance, Fc gamma R-mediated phagocytosis, Regulation of actin cytoskeleton |
| Gene Summary | There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by this gene is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2a) differs in the 5' UTR and coding region as well as the 3' UTR and coding region, compared to variant 1. The resulting isoform (2a) is shorter and has distinct N- and C-termini compared to isoform 1. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217705 | LIMK2 (Myc-DDK-tagged)-Human LIM domain kinase 2 (LIMK2), transcript variant 2a |
CNY 4,752.00 |
|
| RC217705L3 | Lenti-ORF clone of LIMK2 (Myc-DDK-tagged)-Human LIM domain kinase 2 (LIMK2), transcript variant 2a |
CNY 7,510.00 |
|
| RC217705L4 | Lenti-ORF clone of LIMK2 (mGFP-tagged)-Human LIM domain kinase 2 (LIMK2), transcript variant 2a |
CNY 7,510.00 |
|
| RG217705 | LIMK2 (tGFP-tagged) - Human LIM domain kinase 2 (LIMK2), transcript variant 2a |
CNY 6,352.00 |
|
| SC116659 | LIMK2 (untagged)-Human LIM domain kinase 2 (LIMK2), transcript variant 2a |
CNY 4,760.00 |
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