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LIM kinase 2 (LIMK2) (NM_005569) Human Untagged Clone
CAT＃: SC116659
LIMK2 (untagged)-Human LIM domain kinase 2 (LIMK2), transcript variant 2a
CNY 4,760.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_005569 edited
ATGTCCGCGCTGGCGGGTGAAGATGTCTGGAGGTGTCCAGGCTGTGGGGACCACATTGCT CCAAGCCAGATATGGTACAGGACTGTCAACGAAACCTGGCACGGCTCTTGCTTCCGGTGT TCAGAATGCCAGGATTCCCTCACCAACTGGTACTATGAGAAGGATGGGAAGCTCTACTGC CCCAAGGACTACTGGGGGAAGTTTGGGGAGTTCTGTCATGGGTGCTCCCTGCTGATGACA GGGCCTTTTATGGTGGCTGGGGAGTTCAAGTACCACCCAGAGTGCTTTGCCTGTATGAGC TGCAAGGTGATCATTGAGGATGGGGATGCATATGCACTGGTGCAGCATGCCACCCTCTAC TGTGGGAAGTGCCACAATGAGGTGGTGCTGGCACCCATGTTTGAGAGACTCTCCACAGAG TCTGTTCAGGAGCAGCTGCCCTACTCTGTCACGCTCATCTCCATGCCGGCCACCACTGAA GGCAGGCGGGGCTTCTCCGTGTCCGTGGAGAGTGCCTGCTCCAACTACGCCACCACTGTG CAAGTGAAAGAGGTCAACCGGATGCACATCAGTCCCAACAATCGAAACGCCATCCACCCT GGGGACCGCATCCTGGAGATCAATGGGACCCCCGTCCGCACACTTCGAGTGGAGGAGGTG GAGGATGCAATTAGCCAGACGAGCCAGACACTTCAGCTGTTGATTGAACATGACCCCGTC TCCCAACGCCTGGACCAGCTGCGGCTGGAGGCCCGGCTCGCTCCTCACATGCAGAATGCC GGACACCCCCACGCCCTCAGCACCCTGGACACCAAGGAGAATCTGGAGGGGACACTGAGG AGACGTTCCCTAAGGCGCAGTAACAGTATCTCCAAGTCCCCTGGCCCCAGCTCCCCAAAG GAGCCCCTGCTGTTCAGCCGTGACATCAGCCGCTCAGAATCCCTTCGTTGTTCCAGCAGC TATTCACAGCAGATCTTCCGGCCCTGTGACCTAATCCATGGGGAGGTCCTGGGGAAGGGC TTCTTTGGGCAGGCTATCAAGGTGACACACAAAGCCACGGGCAAAGTGATGGTCATGAAA GAGTTAATTCGATGTGATGAGGAGACCCAGAAAACTTTTCTGACTGAGGTGAAAGTGATG CGCAGCCTGGACCACCCCAATGTGCTCAAGTTCATTGGTGTGCTGTACAAGGATAAGAAG CTGAACCTGCTGACAGAGTACATTGAGGGGGGCACACTGAAGGACTTTCTGCGCAGTATG GATCCGTTCCCCTGGCAGCAGAAGGTCAGGTTTGCCAAAGGAATCGCCTCCGGAATGGCC TATTTGCACTCTATGTGCATCATCCACCGGGATCTGAACTCGCACAACTGCCTCATCAAG TTGGACAAGACTGTGGTGGTGGCAGACTTTGGGCTGTCACGGCTCATAGTGGAAGAGAGG AAAAGGGCCCCCATGGAGAAGGCCACCACCAAGAAACGCACCTTGCGCAAGAACGACCGC AAGAAGCGCTACACGGTGGTGGGAAACCCCTACTGGATGGCCCCTGAGATGCTGAACGGA AAGAGCTATGATGAGACGGTGGATATCTTCTCCTTTGGGATCGTTCTCTGTGAGATCATT GGGCAGGTGTATGCAGATCCTGACTGCCTTCCCCGAACACTGGACTTTGGCCTCAACGTG AAGCTTTTCTGGGAGAAGTTTGTTCCCACAGATTGTCCCCCGGCCTTCTTCCCGCTGGCC GCCATCTGCTGCAGACTGGAGCCTGAGAGCAGACCAGCATTCTCGAAATTGGAGGACTCC TTTGAGGCCCTCTCCCTGTACCTGGGGGAGCTGGGCATCCCGCTGCCTGCAGAGCTGGAG GAGTTGGACCACACTGTGAGCATGCAGTACGGCCTGACCCGGGACTCACCTCCCTAG >OriGene 5' read for NM_005569 unedited
GTTCNCATTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGCACGAGGGAACTAAGGG AGCTGCTGTGTCCCCCGCCTCCTCCTCCCCATTTCCGCGCTCCCGGGACCATGTCCGCGC TGGCGGGTGAAGATGTCTGGAGGTGTCCAGGCTGTGGGGACCACATTGCTCCAAGCCAGA TATGGTACAGGACTGTCAACGAAACCTGGCACGGCTCTTGCTTCCGGTGTTCAGAATGCC AGGATTCCCTCACCAACTGGTACTATGAGAAGGATGGGAAGCTCTACTGCCCCAAGGACT ACTGGGGGAAGTTTGGGGAGTTCTGTCATGGGTGCTCCCTGCTGATGACAGGGCCTTTTA TGGTGGCTGGGGAGTTCAAGTACCACCCAGAGTGCTTTGCCTGTATGAGCTGCAAGGTGA TCATTGAGGATGGGGATGCATATGCACTGGTGCAGCATGCCACCCTCTACTGTGGGAAGT GCCACAATGAGGTGGTGCTGGCACCCATGTTTGAGAGACTCTCCACAGAGTCTGTTCAGG AGCAGCTGCCCTACTCTGTCACGCTCATCTCCATGCCGGCCACCACTGAAGGCAGGCGGG GCTTCTCCGTGTCCGTGGAGAGTGCCTGCTCCAACTACGCCACCACTGTGCAAGTGAAAG AGGTCAACCGGATGCACATCAGTCCCAACAATCGAAACGCCATCCACCCTTGGGACCGCA TCCTGGAGATCAATGGGACCCCCGTCCGCACACTTCNAGTGGANGAGGTGNGAGATGCAA TTAGCCAGACGAGCCAGACACTNTCAGCTGTGATTGAACATGACCCCGTCTCCAACGCCT GGACCAGCTGCGGCTGGNAGCCCNGCTCGCTNCTCACATGCAGAAATGCCGACACCCNCA CGCCCTCAGCACCCTGGACACAAGN >OriGene 3' read for NM_005569 unedited
NTTTTACTCTGGACCCGCGGCCGCAATCTAGNGATCGGTTTTTTTTTTTTTTTTTTTATG TTAACTGCTTAATTTATTTAGTCTGATCAATGGCCCAGTTCAGGCCCACCACTCCTACAC TTGCTGGGGACAGCCAGGAAACAACCAGCCACACAATGCTGAACTGTGCTGCTGCTGGGA GTCCAGGGCTCAGCCCTAAAGCAAGCTTGCAAACTTCACACATAAGTACAGTCTATATAG CAAGTAAACTCTGACCAGAGATGACATCTGGTCCCACAACTCATCAGGTCTATGTACAAT ATTTCACATACCACCCAATAGATAAGATAATATTAACAGCAACCACTCTCCTTTATCAAT TCCCCCTGCTCCAATACAACCACCACACATTGCATTAATACCCCAAACCCATTCCCAATT TATTAAATATGGTGCAAGCTCATAGACACTTAGAAGAGGCAAATCTAGTTGTGATGAAGA GTTCCTAGAGCTCTGGGAGCCAAGATGGAGGTTCCCCAGTACCTGCACATGTGGCTCAGG AGGATGCTGCCCAGGAGCTAATGAGTCGGGAGAGCAAACATGGGAGGTAGAAGTCAAATG GCCCAGCTCAGGGAGCTATCTCTCTCAGCATATCAGGTTTGAGACTCTGCCGACACCTGT TTCCAGCCCAAGCTGCTGCCTAAACCAACACTGCTGCAAGGTGACCCGTGGTTGCCTGAA TTCCTGACCACAGATGCTGGGACCCCCGCTGCGCCCGCTGAAACACCAGCGCCGCAACGA CCCCCGTGGCTCGTAGATCGCTGCACTTCCTCCAGACGTATCGAGCCGCCCGTACAGCCC GCGCCCCGACTGAACGATCCACCGACTATCCGCCTCTGTTAAGGCCCACCCCTTTAGAGA CGAGACCAGGACGGCAG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_005569 |
| Insert Size | 4690 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005569.3, NP_005560.1 |
| RefSeq Size | 3701 bp |
| RefSeq ORF | 1917 bp |
| Locus ID | 3985 |
| UniProt ID | P53671 |
| Domains | pkinase, TyrKc, PDZ, LIM, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Axon guidance, Fc gamma R-mediated phagocytosis, Regulation of actin cytoskeleton |
| Gene Summary | There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by this gene is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2a) differs in the 5' UTR and coding region as well as the 3' UTR and coding region, compared to variant 1. The resulting isoform (2a) is shorter and has distinct N- and C-termini compared to isoform 1. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217705 | LIMK2 (Myc-DDK-tagged)-Human LIM domain kinase 2 (LIMK2), transcript variant 2a |
CNY 4,752.00 |
|
| RC217705L3 | Lenti-ORF clone of LIMK2 (Myc-DDK-tagged)-Human LIM domain kinase 2 (LIMK2), transcript variant 2a |
CNY 7,510.00 |
|
| RC217705L4 | Lenti-ORF clone of LIMK2 (mGFP-tagged)-Human LIM domain kinase 2 (LIMK2), transcript variant 2a |
CNY 7,510.00 |
|
| RG217705 | LIMK2 (tGFP-tagged) - Human LIM domain kinase 2 (LIMK2), transcript variant 2a |
CNY 6,352.00 |
|
| SC323590 | LIMK2 (untagged)-Kinase deficient mutant (K360M) of Human LIM domain kinase 2 (LIMK2), transcript variant 2a |
CNY 4,760.00 |
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