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MYLK3 (NM_182493) Human Untagged Clone
CAT＃: SC323576
MYLK3 (untagged)-Kinase deficient mutant (K520M) of Human myosin light chain kinase 3 (MYLK3)
CNY 5,832.00
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | caMLCK; MLCK; MLCK2 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323576 sequence for NM_182493 edited (data generated by NextGen Sequencing)
ATGTCAGGAACCTCCAAGGAGAGTCTGGGGCATGGGGGGCTGCCAGGGTTGGGCAAGACC TGCTTAACAACCATGGACACAAAGCTGAACATGCTGAACGAGAAGGTGGACCAGCTCCTG CACTTCCAAGAAGATGTCACAGAGAAGTTGCAGAGCATGTGCCGAGACATGGGCCACCTG GAGCGGGGCCTGCACAGGCTGGAGGCCTCCCGGGCACCGGGCCCGGGCGGGGCTGATGGG GTTCCCCACATTGACACCCAGGCTGGGTGGCCCGAGGTCCTGGAGCTGGTGAGGGCCATG CAGCAGGATGCGGCCCAGCACGGTGCCAGGCTGGAGGCCCTCTTCAGGATGGTGGCTGCG GTGGACAGGGCCATCGCTTTGGTGGGGGCCACGTTCCAGAAATCAAAGGTGGCGGATTTC CTCATGCAGGGGCGTGTGCCCTGGAGGAGAGGCAGCCCAGGTGACAGCCCTGAGGAGAAT AAAGAGCGAGTGGAAGAAGAGGGAGGAAAACCAAAGCATGTGCTGAGCACCAGTGGGTTG CAGTCTGATGCCAGGGAGCCTGGGGAAGAGAGCCAGAAGGCGGACGTGCTGGAGGGGACA GCGGAGAGGCTGCCCCCCATCAGAGCGTCAGGGCTGGGAGCTGACCCCGCCCAGGCAGTG GTCTCACCGGGCCAGGGAGATGGTGTTCCTGGCCCAGCCCAGGCATTCCCTGGCCACCTG CCCCTGCCCACAAAGGTGGAAGCCAAGGCTCCTGAGACACCCAGCGAGAACCTCAGGACT GGCCTGGAATTGGCTCCAGCACCCGGCAGGGTCAATGTGGTCTCCCCGAGCCTGGAGGTT GCACCAGGTGCAGGACAAGGAGCATCGTCCAGCAGGCCTGACCCTGAGCCCTTAGAGGAA GGCACGAGGCTGACTCCAGGGCCTGGCCCTCAGTGCCCAGGGCCTCCAGGGCTGCCAGCC CAGGCCAGGGCAACCCACAGTGGTGGAGAAACACCTCCAAGGATCTCCATCCACATACAA GAGATGGATACTCCTGGGGAGATGCTGATGACAGGCAGGGGCAGCCTTGGACCCACCCTC ACCACAGAGGCTCCAGCAGCTGCCCAGCCAGGCAAGCAGGGCCCACCTGGGACCGGGCGC TGCCTCCAAGCCCCTGGGACTGAGCCCGGAGAACAGACCCCTGAAGGAGCCAGAGAGCTC TCCCCGCTGCAGGAGAGCAGCAGCCCCGGGGGAGTGAAGGCAGAGGAGGAGCAAAGGGCT GGGGCCGAGCCTGGCACGAGACCAAGCTTGGCCAGGAGTGACGACAATGACCACGAGGTT GGGGCCCTGGGCCTGCAGCAGGGCAAAAGCCCAGGGGCGGGAAACCCTGAGCCTGAGCAG GACTGTGCAGCCAGGGCTCCGGTGAGAGCTGAAGCAGTAAGGAGGATGCCCCCAGGCGCC GAGGCTGGCAGCGTGGTTCTGGATGACAGTCCGGCCCCACCAGCTCCTTTTGAACACCGG GTAGTGAGCGTCAAGGAGACCTCCATCTCTGCGGGTTACGAGGTGTGCCAGCACGAAGTC TTGGGAGGGGGTCGGTTTGGCCAGGTCCACAGGTGCACAGAGAAGTCCACAGGCCTCCCA CTGGCTGCCAWGATCATCAAAGTGAAGAGCGCCAAGGACCGGGAGGACGTGAAGAACGAG ATCAACATCATGAACCAGCTCAGCCACGTGAACCTGATCCAGCTCTATGACGCCTTCGAG AGCAAGCACAGCTGCACCCTTGTCATGGAGTACGTGGACGGGGGTGAGCTCTTCGACCGG ATCACAGATGAGAAGTACCACCTGACTGAGCTGGATGTGGTCCTGTTCACCAGGCAGATC TGTGAGGGTGTGCATTACCTGCACCAGCACTACATCCTGCACCTGGACCTCAAGCCGGAG AACATATTGTGCGTCAATCAGACAGGACATCAAATTAAGATCATTGACTTTGGGCTGGCC AGAAGGTACAAGCCTCGAGAGAAGCTGAAGGTGAACTTCGGCACTCCTGAGTTCCTGGCC CCAGAAGTCGTCAATTATGAGTTTGTCTCATTCCCCACAGACATGTGGAGTGTGGGAGTC ATCACCTACATGCTACTCAGTGGCTTGTCCCCATTTCTAGGGGAAACAGATGCAGAGACC ATGAATTTCATTGTAAACTGTAGCTGGGATTTTGATGCTGACACCTTTGAAGGGCTCTCG GAGGAGGCCAAGGACTTTGTTTCCCGGTTGCTGGTCAAAGAGAAGAGCTGCAGAATGAGT GCCACACAGTGCCTGAAACACGAGTGGCTGAATAATTTGCCTGCCAAAGCTTCAAGATCC AAAACTCGTCTCAAATCCCAACTACTGCTGCAGAAATACATAGCTCAAAGAAAATGGAAG AAACATTTCTATGTGGTGACTGCTGCCAACAGGTTAAGGAAATTTCCAACTTCTCCCTAA Clone variation with respect to NM_182493.2 538 g=>t;1631 a=>w >OriGene 5' read for mutant NM_182493 unedited
CACCCCCCCCGTTGAAACCCAAGGGCCGGGAGGCGTGGCAGAAAAAAGGTCTACACAAGCCGAGCTCGTT TAGGGAACCGAAAAAATTCCGTCCACCACTCACTATAGGGCGGGCGCCAATTCCGCACGAGGCTGACAGC AGTCTGTAAACTACGGAGGACAATGACCTTGCACACACCAATGCCTGAGTGAAAACCATGGGTCTGTGCC TCTCCTTATTCCCTCCTCATGCCCTTGTCCGCCCTTCCCACCGGTTTGGAACCTCCCAGAGAGTCTGGGG GCAGGGGGGCGCCGCCAGGGATGAGCAAAACCTGCTTAACACACTGGACCCAAAACTGAACTTGCTTATT GAATATGGTGAACCCCATCCCGCACTCCCAAAAAAATTTAAAACACCAATTTCCAAGACATGTCCCCAGA AATAGGCCCACGTGAACAGGGGCCTTGAAAGCCTTGAAAGACCCCCCCGGCACCGGGCCCCAGACAGGGA TAAAAGGGCTCCCCCAAAATGACACCAAACTAGGATGTTCCAGGTTCTCCAAACC >SC323576 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
AWATCTCTGCGGATMGAGGTGTGCCAGCACGAAGTCTTGGGAGGGGGTCGGTTTGGCCAGGTCCACAGGT GCACAGAGAAGTCCACAGGCCTCCCACTGGCTGCCATGATCATCAAAGTGAAGAGCGCCAAGGACCGGGA GGACGTGAAGAACGAGATCAACATCATGAACCAGCTCAGCCACGTGAACCTGATCCAGCTCTATGACGCC TTCGAGAGCAAGCACAGCTGCACCCTTGTCATGGAGTACGTGGAC |
| Restriction Sites | Please inquire |
| ACCN | NM_182493 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_182493.1, NP_872299.1 |
| RefSeq Size | 2760 bp |
| RefSeq ORF | 2388 bp |
| Locus ID | 91807 |
| UniProt ID | Q32MK0 |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Calcium signaling pathway, Focal adhesion, Regulation of actin cytoskeleton, Vascular smooth muscle contraction |
| Gene Summary | Phosphorylation of cardiac myosin heavy chains (see MYH7B, MIM 609928) and light chains (see MYL2, MIM 160781) by a kinase, such as MYLK3, potentiates the force and rate of cross-bridge recruitment in cardiac myocytes (Chan et al., 2008 [PubMed 18202317]).[supplied by OMIM, Jul 2008] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer protein (isoform 1). |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222246 | MYLK3 (Myc-DDK-tagged)-Human myosin light chain kinase 3 (MYLK3) |
CNY 6,000.00 |
|
| RC222246L1 | Lenti-ORF clone of MYLK3 (Myc-DDK-tagged)-Human myosin light chain kinase 3 (MYLK3) |
CNY 8,400.00 |
|
| RC222246L2 | Lenti-ORF clone of MYLK3 (mGFP-tagged)-Human myosin light chain kinase 3 (MYLK3) |
CNY 7,700.00 |
|
| RC222246L3 | Lenti-ORF clone of MYLK3 (Myc-DDK-tagged)-Human myosin light chain kinase 3 (MYLK3) |
CNY 8,400.00 |
|
| RC222246L4 | Lenti-ORF clone of MYLK3 (mGFP-tagged)-Human myosin light chain kinase 3 (MYLK3) |
CNY 7,700.00 |
|
| RG222246 | MYLK3 (tGFP-tagged) - Human myosin light chain kinase 3 (MYLK3) |
CNY 7,600.00 |
|
| SC307402 | MYLK3 (untagged)-Human myosin light chain kinase 3 (MYLK3) |
CNY 7,220.00 |
|
| SC318299 | MYLK3 (untagged)-Human myosin light chain kinase 3 (MYLK3) |
CNY 7,220.00 |
