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TYK2 (NM_003331) Human Untagged Clone
CAT＃: SC323566
TYK2 (untagged)-Kinase deficient mutant (K930M) of Human tyrosine kinase 2 (TYK2)
CNY 8,272.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | IMD35; JTK1 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_003331, the custom clone sequence may differ by one or more nucleotides
ATGCCTCTGCGCCACTGGGGGATGGCCAGGGGCAGTAAGCCCGTTGGGGATGGAGCCCAGCCCATGGCTG CCATGGGAGGCCTGAAGGTGCTTCTGCACTGGGCTGGTCCAGGCGGCGGGGAGCCCTGGGTCACTTTCAG TGAGTCATCGCTGACAGCTGAGGAAGTCTGCATCCACATTGCACATAAAGTTGGTATCACTCCTCCTTGC TTCAATCTCTTTGCCCTCTTCGATGCTCAGGCCCAAGTCTGGTTGCCCCCAAACCACATCCTAGAGATCC CCAGAGATGCAAGCCTGATGCTATATTTCCGCATAAGGTTTTATTTCCGGAACTGGCATGGCATGAATCC TCGGGAACCGGCTGTGTACCGTTGTGGGCCCCCAGGAACCGAGGCATCCTCAGATCAGACAGCACAGGGG ATGCAACTCCTGGACCCAGCCTCATTTGAGTACCTCTTTGAGCAGGGCAAGCATGAGTTTGTGAATGACG TGGCATCACTGTGGGAGCTGTCGACCGAGGAGGAGATCCACCACTTTAAGAATGAGAGCCTGGGCATGGC CTTTCTGCACCTCTGTCACCTCGCTCTCCGCCATGGCATCCCCCTGGAGGAGGTGGCCAAGAAGACCAGC TTCAAGGACTGCATCCCGCGCTCCTTCCGCCGGCATATCCGGCAGCACAGCGCCCTGACCCGGCTGCGCC TTCGGAACGTCTTCCGCAGGTTCCTGCGGGACTTCCAGCCGGGCCGACTCTCCCAGCAGATGGTCATGGT CAAATACCTAGCCACACTCGAGCGGCTGGCACCCCGCTTCGGCACAGAGCGTGTGCCCGTGTGCCACCTG AGGCTGCTGGCCCAGGCCGAGGGGGAGCCCTGCTACATCCGGGACAGTGGGGTGGCCCCTACAGACCCTG GCCCTGAGTCTGCTGCTGGGCCCCCAACCCACGAGGTGCTGGTGACAGGCACTGGTGGCATCCAGTGGTG GCCAGTAGAGGAGGAGGTGAACAAGGAGGAGGGTTCTAGTGGCAGCAGTGGCAGGAACCCCCAAGCCAGC CTGTTTGGGAAGAAGGCCAAGGCTCACAAGGCAGTCGGCCAGCCGGCAGACAGGCCGCGGGAGCCACTGT GGGCCTACTTCTGTGACTTCCGGGACATCACCCACGTGGTGCTGAAAGAGCACTGTGTCAGCATCCACCG GCAGGACAACAAGTGCCTGGAGCTGAGCTTGCCTTCCCGGGCTGCGGCGCTGTCCTTCGTGTCGCTGGTG GACGGCTATTTCCGCCTGACGGCCGACTCCAGCCACTACCTGTGCCACGAGGTGGCTCCCCCACGGCTGG TGATGAGCATCCGGGATGGGATCCACGGACCCCTGCTGGAGCCATTTGTGCAGGCCAAGCTGCGGCCCGA GGACGGCCTGTACCTCATTCACTGGAGCACCAGCCACCCCTACCGCCTGATCCTCACAGTGGCCCAGCGT AGCCAGGCACCAGACGGCATGCAGAGCTTGCGGCTCCGAAAGTTCCCCATTGAGCAGCAGGACGGGGCCT TCGTGCTGGAGGGCTGGGGCCGGTCCTTCCCCAGCGTTCGGGAACTTGGGGCTGCCTTGCAGGGCTGCTT GCTGAGGGCCGGGGATGACTGCTTCTCTCTGCGTCGCTGTTGCCTGCCCCAACCAGGAGAAACCTCCAAT CTCATCATCATGCGGGGGGCTCGGGCCAGCCCCAGGACACTCAACCTCAGCCAGCTCAGCTTCCACCGGG TTGACCAGAAGGAGATCACCCAGCTGTCCCACTTGGGCCAGGGCACAAGGACCAACGTGTATGAGGGCCG CCTGCGAGTGGAGGGCAGCGGGGACCCTGAGGAGGGCAAGATGGATGACGAGGACCCCCTCGTGCCTGGC AGGGACCGTGGGCAGGAGCTACGAGTGGTGCTCAAAGTGCTGGACCCTAGTCACCATGACATCGCCCTGG CCTTCTACGAGACAGCCAGCCTCATGAGCCAGGTCTCCCACACGCACCTGGCCTTCGTGCATGGCGTCTG TGTGCGCGGCCCTGAAAATATCATGGTGACAGAGTACGTGGAGCACGGACCCCTGGATGTGTGGCTGCGG AGGGAGCGGGGCCATGTGCCCATGGCTTGGAAGATGGTGGTGGCCCAGCAGCTGGCCAGCGCCCTCAGCT ACCTGGAGAACAAGAACCTGGTTCATGGTAATGTGTGTGGCCGGAACATCCTGCTGGCCCGGCTGGGGTT GGCAGAGGGCACCAGCCCCTTCATCAAGCTGAGTGATCCTGGCGTGGGCCTGGGCGCCCTCTCCAGGGAG GAGCGGGTGGAGAGGATCCCCTGGCTGGCCCCCGAATGCCTACCAGGTGGGGCCAACAGCCTAAGCACCG CCATGGACAAGTGGGGGTTTGGCGCCACCCTCCTGGAGATCTGCTTTGACGGAGAGGCCCCTCTGCAGAG CCGCAGTCCCTCCGAGAAGGAGCATTTCTACCAGAGGCAGCACCGGCTGCCCGAGCCCTCCTGCCCACAG CTGGCCACACTCACCAGCCAGTGTCTGACCTATGAGCCAACCCAGAGGCCATCATTCCGCACCATCCTGC GTGACCTCACCCGGCTGCAGCCCCACAATCTTGCTGACGTCTTGACTGTGAACCCGGACTCACCGGCGTC GGACCCTACGGTTTTCCACAAGCGCTATTTGAAAAAGATCCGAGATCTGGGCGAGGGTCACTTCGGCAAG GTCAGCTTGTACTGCTACGATCCGACCAACGACGGCACTGGCGAGATGGTGGCGGTGAAAGCCCTCAAGG CAGACTGCGGCCCCCAGCACCGCTCGGGCTGGAAGCAGGAGATTGACATTCTGCGCACGCTCTACCACGA GCACATCATCAAGTACAAGGGCTGCTGCGAGGACCAAGGCGAGAAGTCGCTGCAGCTGGTCATGGAGTAC GTGCCCCTGGGCAGCCTCCGAGACTACCTGCCCCGGCACAGCATCGGGCTGGCCCAGCTGCTGCTCTTCG CCCAGCAGATCTGCGAGGGCATGGCCTATCTGCACGCGCAGCACTACATCCACCGAGACCTAGCCGCGCG CAACGTGCTGCTGGACAACGACAGGCTGGTCAAGATCGGGGACTTTGGCCTAGCCAAGGCCGTGCCCGAA GGCCACGAGTACTACCGCGTGCGCGAGGATGGGGACAGCCCCGTGTTCTGGTATGCCCCAGAGTGCCTGA AGGAGTATAAGTTCTACTATGCGTCAGATGTCTGGTCCTTCGGGGTGACCCTGTATGAGCTGCTGACGCA CTGTGACTCCAGCCAGAGCCCCCCCACGAAATTCCTTGAGCTCATAGGCATTGCTCAGGGTCAGATGACA GTTCTGAGACTCACTGAGTTGCTGGAACGAGGGGAGAGGCTGCCACGGCCCGACAAATGTCCCTGTGAGG TCTATCATCTCATGAAGAACTGCTGGGAGACAGAGGCGTCCTTTCGCCCAACCTTCGAGAACCTCATACC CATTCTGAAGACAGTCCATGAGAAGTACCAAGGCCAGGCCCCTTCAGTGTTCAGCGTGTGCTGA >OriGene 5' read for mutant NM_003331 unedited
CCGCCCGTCCAGCACTGGGCGGTAGGCGCTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGA ACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCGCGGCCGGAGG TCCTCAGGAAGAAGCCGCGGGGACTGGCTGCGCTTGACAGGCTGCACTTGGATGGGAGCGGCTGGTGCCT CGAGATTGCTCTGATGCCGGGTTCTAGGGTGAATATGAAATAACAATAACTCATGTACAGTGTTACAGTT ACTTTGGGGGCAACCCACAAATGAGTCCTAAAAAAAAAAATAAAACCAAAAATAAGGGGGTTGTGTGGTT GTTTAGGCCATATCCAGTCCAATACCTCTGATTCTATTAAAGGAAAAATTACTGAGGGGAGGTTGAAAAA AAAAAAAAAAAAAAATTAACCATCCGCACAAC >SC323566 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
AWGGCGAAGGTCMTTCGGCAGGTCAGCTTGTACTGCTACGATCCGACCAACGACGGCACTGGCGAGATGG TGGCGGTGATGGCCCTCAAGGCAGACTGCGGCCCCCAGCACCGCTCGGGCTGGAAGCAGGAGATTGACAT TCTGCGCACGCTCTACCACGAGCACATCATCAAGTACAAGGGCTGCTGCGAGGACCAAGAAATTCCTTGA GCTCATAGGCATTGCTCAGGGTCAGATGACAGTTCTGAGACTCAC |
| Restriction Sites | Please inquire |
| ACCN | NM_003331 |
| Insert Size | 4200 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003331.3, NP_003322.2 |
| RefSeq Size | 4224 bp |
| RefSeq ORF | 3564 bp |
| Locus ID | 7297 |
| UniProt ID | P29597 |
| Domains | B41, pkinase, SH2, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Jak-STAT signaling pathway |
| Gene Summary | This gene encodes a member of the tyrosine kinase and, more specifically, the Janus kinases (JAKs) protein families. This protein associates with the cytoplasmic domain of type I and type II cytokine receptors and promulgate cytokine signals by phosphorylating receptor subunits. It is also a component of both the type I and type III interferon signaling pathways. As such, it may play a role in anti-viral immunity. A mutation in this gene has been associated with Immunodeficiency 35. [provided by RefSeq, Sep 2020] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204351 | TYK2 (Myc-DDK-tagged)-Human tyrosine kinase 2 (TYK2) |
CNY 12,400.00 |
|
| RC204351L1 | Lenti ORF clone of Human tyrosine kinase 2 (TYK2), Myc-DDK-tagged |
CNY 14,800.00 |
|
| RC204351L2 | Lenti ORF clone of Human tyrosine kinase 2 (TYK2), mGFP tagged |
CNY 12,070.00 |
|
| RC204351L3 | Lenti ORF clone of Human tyrosine kinase 2 (TYK2), Myc-DDK-tagged |
CNY 12,070.00 |
|
| RC204351L4 | Lenti ORF clone of Human tyrosine kinase 2 (TYK2), mGFP tagged |
CNY 14,800.00 |
|
| RG204351 | TYK2 (tGFP-tagged) - Human tyrosine kinase 2 (TYK2) |
CNY 14,000.00 |
|
| SC128128 | TYK2 (untagged)-Human tyrosine kinase 2 (TYK2) |
CNY 8,272.00 |
