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TEC (NM_003215) Human Untagged Clone
CAT＃: SC323542
TEC (untagged)-Kinase deficient mutant (K398M) of Human tec protein tyrosine kinase (TEC)
CNY 7,056.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | PSCTK4 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_003215, the custom clone sequence may differ by one or more nucleotides
ATGAATTTTAACACTATTTTGGAGGAGATTCTTATTAAAAGGTCACAGCAGAAAAAGAAGACATCGCCCT TAAACTACAAAGAGAGACTTTTTGTACTTACAAAGTCCATGCTAACCTACTATGAGGGTCGAGCAGAGAA GAAATACAGAAAGGGGTTTATTGATGTTTCAAAAATCAAGTGTGTGGAAATAGTGAAGAATGATGATGGT GTCATTCCCTGTCAAAATAAGTATCCATTTCAGGTTGTTCATGATGCTAACACACTTTACATTTTTGCAC CTAGTCCACAAAGCAGGGACCTGTGGGTGAAGAAGTTAAAAGAAGAAATAAAGAACAACAATAATATTAT GATTAAATATCATCCTAAATTCTGGACAGATGGAAGTTATCAGTGTTGTAGACAAACTGAAAAATTAGCA CCCGGATGTGAAAAATACAATCTTTTTGAGAGCAGTATAAGAAAAGCACTACCTCCAGCACCAGAAACAA AGAAGCGAAGGCCTCCCCCACCAATTCCACTAGAAGAAGAAGATAATAGTGAAGAAATCGTTGTAGCCAT GTATGATTTCCAAGCAGCAGAAGGACATGATCTCAGATTAGAGAGAGGCCAAGAGTATCTCATTTTAGAA AAGAATGATGTTCATTGGTGGAGAGCAAGAGATAAATATGGGAATGAAGGATATATCCCAAGTAATTACG TAACGGGAAAGAAATCAAACAACTTAGATCAATATGAATGGTATTGCAGAAATATGAATAGAAGCAAGGC AGAGCAACTCCTCCGCAGTGAAGATAAAGAAGGTGGTTTTATGGTAAGGGATTCCAGTCAACCAGGCTTG TACACAGTCTCCCTTTATACCAAGTTTGGAGGAGAAGGTTCATCGGGTTTTAGGCATTATCATATAAAGG AAACAACAACATCTCCAAAGAAGTATTACCTAGCTGAAAAACATGCTTTTGGCTCCATTCCTGAGATTAT TGAATATCATAAGCACAATGCAGCAGGACTTGTCACCAGGCTTCGGTACCCAGTTAGTGTGAAAGGGAAG AATGCACCCACCACTGCAGGATTCAGCTATGAGAAATGGGAGATTAACCCTTCAGAACTGACCTTTATGA GGGAATTGGGAAGTGGACTGTTTGGAGTGGTGAGGCTTGGCAAATGGCGAGCCCAGTACAAAGTCGCAAT CAAAGCTATTCGGGAAGGTGCAATGTGCGAGGAGGACTTTATAGAAGAAGCTAAAGTGATGATGAAACTG ACACACCCGAAGTTAGTGCAGCTTTATGGTGTGTGCACCCAGCAGAAACCAATATACATTGTTACTGAGT TCATGGAAAGGGGCTGCCTTCTGAATTTCCTCCGACAGAGACAAGGTCATTTCAGTAGAGACGTACTGCT GAGCATGTGTCAGGATGTGTGTGAAGGGATGGAGTATCTGGAGAGAAACAGCTTCATCCACAGAGATCTG GCTGCCAGAAATTGTCTAGTAAGTGAGGCGGGAGTTGTAAAAGTATCTGATTTTGGAATGGCCAGGTATG TTCTGGATGATCAGTACACAAGTTCTTCTGGTGCTAAGTTTCCTGTGAAGTGGTGTCCACCTGAAGTGTT TAATTACAGCCGCTTCAGCAGCAAATCAGATGTCTGGTCATTTGGTGTTTTAATGTGGGAAGTATTCACG GAAGGCAGAATGCCTTTTGAAAAATACACCAATTATGAAGTGGTAACCATGGTTACTCGAGGCCACCGAC TCTACCAGCCGAAGTTGGCGTCCAACTATGTGTATGAGGTGATGCTGAGATGTTGGCAGGAGAAACCAGA GGGAAGGCCTTCTTTCGAAGATCTGCTGCGCACAATAGATGAACTAGTTGAATGTGAAGAAACTTTTGGA AGATAA >OriGene 5' read for mutant NM_003215 unedited
CCGCCGTCTGAGCAATGGGCGGTAGGCGCTGTACGGCTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTG AACCGTCAGAATATTGTAATACGACTCACTATAGGGCGGCCGCGAATACGGCACGAGCAGGTCCGCCCAG CAGCCGCGCTTCAGCCAGAATACTGGGAATCTTCAGTGGCAGGAGGAGTAATCAGAAGACGGAGATGAAT TTTAACACTATTTTGGAGGAGATTCTTATTAAAAGGTCACAGCAGAAAAAGAAGACATCGCCCTTAAACT ACAAAGAGAGACTTTTTGTACTTACAAAGTCCATGCTAACCTACTATGAGGGTCGAGCAGAGAAGAAATA CAGAAAGGGGTTTATTGATGTTTCAAAAATCAAGTGTGTGGAAATAGTGAAGATGATGATGGGTGTCATT CCCTGTCAAAATAAGTATCCATTTCAGGTTTGTTCATGATGCTAACACACTTTACATTTTTGCACCTAGT CCAACAAAGCAGGGACTGGTGGGTTGAAGAAGTTAAAGAAGAATTAAGGACCACATTATTTATGATTAAT TCCTCCTAAATTCTGGAACGAAGGGGATTACGTGGTGAAACAACTGAAAATAGCCCGGAGTGAAAATCCA TCTTTTAGAGATGTAAGAAAGCCACACTCCCCCGACCAAACGAAAGCTCCCCCATTCCTAGAGAGATATT TGAAATCTGTGCCCGTGTAATTCCACCAAGACGTCTCGATAAGAAGCCAAGTTCTTTAAGAGGTGTCTTG TGGACCATATTGGATAGAGATCCGTTCTCCGAATCACCTTTCTATGTGCCATTAGAGCCGGACCTCCCTG AATAAGTTGAGGAGTCACTGAATC >SC323542 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
TMGACTTTATGAGGGATTGGGAGTGGACTGTTTGGAGTGGTGAGGCTTGGCAAATGGCGAGCCCAGTACA AAGTCGCAATCATGGCTATTCGGGAAGGTGCAATGTGCGAGGAGGACTTTATAGAAGAAGCTAAAGTGAT GATGAAACTGACACACCCGAAGTTAGTGCAGCTTTATGGTGTGTGCACCCAGCAGAAACCAATATACATT GTTACTGAGTTCATGGAAAGGGGCTGCCTTCTGAATTTCCTCCGA |
| Restriction Sites | Please inquire |
| ACCN | NM_003215 |
| Insert Size | 2600 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003215.1, NP_003206.1 |
| RefSeq Size | 3620 bp |
| RefSeq ORF | 1896 bp |
| Locus ID | 7006 |
| UniProt ID | P42680 |
| Domains | pkinase, SH2, TyrKc, SH3, BTK, PH, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | T cell receptor signaling pathway |
| Gene Summary | The protein encoded by this gene belongs to the Tec family of non-receptor protein-tyrosine kinases containing a pleckstrin homology domain. Tec family kinases are involved in the intracellular signaling mechanisms of cytokine receptors, lymphocyte surface antigens, heterotrimeric G-protein coupled receptors, and integrin molecules. They are also key players in the regulation of the immune functions. Tec kinase is an integral component of T cell signaling and has a distinct role in T cell activation. This gene may be associated with myelodysplastic syndrome. [provided by RefSeq, Jul 2008] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210992 | TEC (Myc-DDK-tagged)-Human tec protein tyrosine kinase (TEC) |
CNY 7,048.00 |
|
| RC210992L1 | Lenti ORF clone of Human tec protein tyrosine kinase (TEC), Myc-DDK-tagged |
CNY 9,448.00 |
|
| RC210992L2 | Lenti ORF clone of Human tec protein tyrosine kinase (TEC), mGFP tagged |
CNY 9,448.00 |
|
| RC210992L3 | Lenti ORF clone of Human tec protein tyrosine kinase (TEC), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC210992L4 | Lenti ORF clone of Human tec protein tyrosine kinase (TEC), mGFP tagged |
CNY 5,890.00 |
|
| RG210992 | TEC (tGFP-tagged) - Human tec protein tyrosine kinase (TEC) |
CNY 8,648.00 |
|
| SC128212 | TEC (untagged)-Human tec protein tyrosine kinase (TEC) |
CNY 7,056.00 |
