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PSKH1 (NM_006742) Human Untagged Clone
CAT＃: SC323538
PSKH1 (untagged)-Kinase deficient mutant (K127M) of Human protein serine kinase H1 (PSKH1)
CNY 3,656.00
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323538 sequence for NM_006742 edited (data generated by NextGen Sequencing)
ATGGGCTGTGGGACAAGCAAGGTCCTTCCCGAGCCACCCAAGGATGTCCAGCTGGATCTG GTCAAGAAGGTGGAGCCCTTCAGTGGCACTAAGAGTGACGTGTACAAGCACTTCATCACA GAGGTGGACAGTGTTGGCCCTGTCAAAGCCGGGTTCCCAGCAGCAAGTCAGTATGCACAC CCCTGCCCCGGTCCCCCGACTGCTGGCCACACGGAGCCTCCCTCAGAACCACCACGCAGG GCCAGGGTAGCTAAGTACAGGGCCAAGTTTGACCCACGTGTTACAGCTAAGTATGACATC AAGGCCCTAATTGGCCGAGGCAGCTTCAGCCGAGTGGTACGTGTAGAGCACCGGGCAACC CGGCAGCCGTATGCCATCATGATGATTGAGACCAAGTACCGGGAGGGGCGGGAGGTGTGT GAGTCGGAGCTGCGTGTGCTGCGTCGGGTGCGTCATGCCAACATCATCCAGCTGGTGGAG GTGTTCGAGACACAGGAGCGGGTGTACATGGTGATGGAGCTGGCCACTGGTGGAGAGCTC TTTGACCGCATCATTGCCAAGGGCTCCTTCACCGAGCGTGACGCCACGCGGGTGCTGCAG ATGGTGCTGGATGGCGTCCGGTATCTGCATGCACTGGGCATCACACACCGAGACCTCAAA CCTGAGAATCTGCTCTACTACCATCCGGGCACTGACTCCAAGATCATCATCACCGACTTC GGCCTGGCCAGTGCTCGCAAGAAGGGTGATGACTGCTTGATGAAGACCACCTGTGGCACG CCTGAGTACATTGCCCCAGAAGTCCTGGTCCGCAAGCCATACACCAACTCAGTGGACATG TGGGCGCTGGGCGTCATTGCCTACATCCTACTCAGTGGCACCATGCCGTTTGAGGATGAC AACCGTACCCGGCTGTACCGGCAGATCCTCAGGGGCAAGTACAGTTACTCTGGGGAGCCC TGGCCTAGTGTGTCCAACCTGGCCAAGGACTTCATTGACCGCCTGCTGACAGTGGACCCT GGAGCCCGTATGACTGCACTGCAGGCCCTGAGGCACCCGTGGGTGGTGAGCATGGCTGCC TCTTCATCCATGAAGAACCTGCACCGCTCCATATCCCAGAACCTCCTTAAACGTGCCTCC TCGCGCTGCCAGAGCACCAAATCTGCCCAGTCCACGCGTTCCAGCCGCTCCACACGCTCC AATAAGTCACGCCGTGTGCGGGAACGGGAGCTGCGGGAGCTCAACCTGCGCTACCAGCAG CAATACAATGGCTGA Clone variation with respect to NM_006742.2 380 a=>t >OriGene 5' read for mutant NM_006742 unedited
ACGGTACAACTAAGCACGAGCGGAGGCTGAACTCGAGGGTAATATAGCAGACACATTAGGCGAACAACAG AACAAAGCAACACGAACAAAACGAGCGGCCGCGAACCCGGCACGAAAAGCGGCGGCGGCGGCGGCGGCCG CTGCCATTGCCCGGAGATGGCCGGCAGAGCCGCCGATACGCCTTATGGCCGGCCTCCCCGCTTTATGTAG ATTGTCTTTTTGGTAAAAAGAAACTGAACTAAACTTATGCCTTGGCTTTTCTTGCGCTCCTTGTTCTGAT TTACCCCGATCTACTCACTAATTCTCAGTTTAACCGCTTGGGTGTAGTGATTTAAGGTCACACCCACACA AAAATAACACACAAATATCATAATACGAAAGCCGAAATCCCCATTAATGTATCAAATTCAGTACTACTAA TACCACCTAGACACCCAAAAAATATAAAAATAATTTAAATTTTAAATAACCAATCAATTATTACATATAA CACCAAATACTAACTACAT >SC323538 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
TAGATATTGGCCGAGGCAGCTTCAGCCGAGTGGTACGTGTAGAGCACCGGGCAACCCGGCAGCCGTATGC CATCATGATGATTGAGACCAAGTACCGGGAGGGGCGGGAGGTGTGTGAGTCGGAGCTGCGTGTGCTGCGT CGGGTGCGTCATGCCAACATCATCCAGCTGGTGGAGGTGTTCGAGACACAGGAGCGGGTGTACATGGTGA TGGAGCTGGCCACTGGTGGAGAGCTCTTTGACCGCATCATTGCCA |
| Restriction Sites | Please inquire |
| ACCN | NM_006742 |
| Insert Size | 4000 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_006742.1, NP_006733.1 |
| RefSeq Size | 3460 bp |
| RefSeq ORF | 1275 bp |
| Locus ID | 5681 |
| UniProt ID | P11801 |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | May be a SFC-associated serine kinase (splicing factor compartment-associated serine kinase) with a role in intranuclear SR protein (non-snRNP splicing factors containing a serine/arginine-rich domain) trafficking and pre-mRNA processing.[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208968 | PSKH1 (Myc-DDK-tagged)-Human protein serine kinase H1 (PSKH1) |
CNY 3,656.00 |
|
| RC208968L1 | Lenti ORF clone of Human protein serine kinase H1 (PSKH1), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC208968L2 | Lenti ORF clone of Human protein serine kinase H1 (PSKH1), mGFP tagged |
CNY 5,890.00 |
|
| RC208968L3 | Lenti ORF clone of Human protein serine kinase H1 (PSKH1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC208968L4 | Lenti ORF clone of Human protein serine kinase H1 (PSKH1), mGFP tagged |
CNY 5,890.00 |
|
| RG208968 | PSKH1 (tGFP-tagged) - Human protein serine kinase H1 (PSKH1) |
CNY 5,256.00 |
|
| SC101252 | PSKH1 (untagged)-Human protein serine kinase H1 (PSKH1) |
CNY 3,656.00 |
