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FLT4 (NM_002020) Human Untagged Clone
CAT＃: SC323508
FLT4 (untagged)-Kinase deficient mutant (K879M) of Human fms-related tyrosine kinase 4 (FLT4), transcript variant 2
CNY 9,048.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CHTD7; FLT-4; FLT41; LMPH1A; LMPHM1; PCL; VEGFR-3; VEGFR3 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>SC323508 representing NM_002020.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCGGCCGCGAATTCGGCACGAGGGTCGGACCCACGCGCAGCGGCCGGAGATGCAGCGGGGCGCCGCGCT GTGCCTGCGACTGTGGCTCTGCCTGGGACTCCTGGACGGCCTGGTGAGTGGCTACTCCATGACCCCCCC GACCTTGAACATCACGGAGGAGTCACACGTCATCGACACCGGTGACAGCCTGTCCATCTCCTGCAGGGG ACAGCACCCCCTCGAGTGGGCTTGGCCAGGAGCTCAGGAGGCGCCAGCCACCGGAGACAAGGACAGCGA GGACACGGGGGTGGTGCGAGACTGCGAGGGCACAGACGCCAGGCCCTACTGCAAGGTGTTGCTGCTGCA CGAGGTACATGCCAACGACACAGGCAGCTACGTCTGCTACTACAAGTACATCAAGGCACGCATCGAGGG CACCACGGCCGCCAGCTCCTACGTGTTCGTGAGAGACTTTGAGCAGCCATTCATCAACAAGCCTGACAC GCTCTTGGTCAACAGGAAGGACGCCATGTGGGTGCCCTGTCTGGTGTCCATCCCCGGCCTCAATGTCAC GCTGCGCTCGCAAAGCTCGGTGCTGTGGCCAGACGGGCAGGAGGTGGTGTGGGATGACCGGCGGGGCAT GCTCGTGTCCACGCCACTGCTGCACGATGCCCTGTACCTGCAGTGCGAGACCACCTGGGGAGACCAGGA CTTCCTTTCCAACCCCTTCCTGGTGCACATCACAGGCAACGAGCTCTATGACATCCAGCTGTTGCCCAG GAAGTCGCTGGAGCTGCTGGTAGGGGAGAAGCTGGTCCTGAACTGCACCGTGTGGGCTGAGTTTAACTC AGGTGTCACCTTTGACTGGGACTACCCAGGGAAGCAGGCAGAGCGGGGTAAGTGGGTGCCCGAGCGACG CTCCCAGCAGACCCACACAGAACTCTCCAGCATCCTGACCATCCACAACGTCAGCCAGCACGACCTGGG CTCGTATGTGTGCAAGGCCAACAACGGCATCCAGCGATTTCGGGAGAGCACCGAGGTCATTGTGCATGA AAATCCCTTCATCAGCGTCGAGTGGCTCAAAGGACCCATCCTGGAGGCCACGGCAGGAGACGAGCTGGT GAAGCTGCCCGTGAAGCTGGCAGCGTACCCCCCGCCCGAGTTCCAGTGGTACAAGGATGGAAAGGCACT GTCCGGGCGCCACAGTCCACATGCCCTGGTGCTCAAGGAGGTGACAGAGGCCAGCACAGGCACCTACAC CCTCGCCCTGTGGAACTCCGCTGCTGGCCTGAGGCGCAACATCAGCCTGGAGCTGGTGGTGAATGTGCC CCCCCAGATACATGAGAAGGAGGCCTCCTCCCCCAGCATCTACTCGCGTCACAGCCGCCAGGCCCTCAC CTGCACGGCCTACGGGGTGCCCCTGCCTCTCAGCATCCAGTGGCACTGGCGGCCCTGGACACCCTGCAA GATGTTTGCCCAGCGTAGTCTCCGGCGGCGGCAGCAGCAAGACCTCATGCCACAGTGCCGTGACTGGAG GGCGGTGACCACGCAGGATGCCGTGAACCCCATCGAGAGCCTGGACACCTGGACCGAGTTTGTGGAGGG AAAGAATAAGACTGTGAGCAAGCTGGTGATCCAGAATGCCAACGTGTCTGCCATGTACAAGTGTGTGGT CTCCAACAAGGTGGGCCAGGATGAGCGGCTCATCTACTTCTATGTGACCACCATCCCCGACGGCTTCAC CATCGAATCCAAGCCATCCGAGGAGCTACTAGAGGGCCAGCCGGTGCTCCTGAGCTGCCAAGCCGACAG CTACAAGTACGAGCATCTGCGCTGGTACCGCCTCAACCTGTCCACGCTGCACGATGCGCACGGGAACCC GCTTCTGCTCGACTGCAAGAACGTGCATCTGTTCGCCACCCCTCTGGCCGCCAGCCTGGAGGAGGTGGC ACCTGGGGCGCGCCACGCCACGCTCAGCCTGAGTATCCCCCGCGTCGCGCCCGAGCACGAGGGCCACTA TGTGTGCGAAGTGCAAGACCGGCGCAGCCATGACAAGCACTGCCACAAGAAGTACCTGTCGGTGCAGGC CCTGGAAGCCCCTCGGCTCACGCAGAACTTGACCGACCTCCTGGTGAACGTGAGCGACTCGCTGGAGAT GCAGTGCTTGGTGGCCGGAGCGCACGCGCCCAGCATCGTGTGGTACAAAGACGAGAGGCTGCTGGAGGA AAAGTCTGGAGTCGACTTGGCGGACTCCAACCAGAAGCTGAGCATCCAGCGCGTGCGCGAGGAGGATGC GGGACGCTATCTGTGCAGCGTGTGCAACGCCAAGGGCTGCGTCAACTCCTCCGCCAGCGTGGCCGTGGA AGGCTCCGAGGATAAGGGCAGCATGGAGATCGTGATCCTTGTCGGTACCGGCGTCATCGCTGTCTTCTT CTGGGTCCTCCTCCTCCTCATCTTCTGTAACATGAGGAGGCCGGCCCACGCAGACATCAAGACGGGCTA CCTGTCCATCATCATGGACCCCGGGGAGGTGCCTCTGGAGGAGCAATGCGAATACCTGTCCTACGATGC CAGCCAGTGGGAATTCCCCCGAGAGCGGCTGCACCTGGGGAGAGTGCTCGGCTACGGCGCCTTCGGGAA GGTGGTGGAAGCCTCCGCTTTCGGCATCCACAAGGGCAGCAGCTGTGACACCGTGGCCGTGATGATGCT GAAAGAGGGCGCCACGGCCAGCGAGCACCGCGCGCTGATGTCGGAGCTCAAGATCCTCATTCACATCGG CAACCACCTCAACGTGGTCAACCTCCTCGGGGCGTGCACCAAGCCGCAGGGCCCCCTCATGGTGATCGT GGAGTTCTGCAAGTACGGCAACCTCTCCAACTTCCTGCGCGCCAAGCGGGACGCCTTCAGCCCCTGCGC GGAGAAGTCTCCCGAGCAGCGCGGACGCTTCCGCGCCATGGTGGAGCTCGCCAGGCTGGATCGGAGGCG GCCGGGGAGCAGCGACAGGGTCCTCTTCGCGCGGTTCTCGAAGACCGAGGGCGGAGCGAGGCGGGCTTC TCCAGACCAAGAAGCTGAGGACCTGTGGCTGAGCCCGCTGACCATGGAAGATCTTGTCTGCTACAGCTT CCAGGTGGCCAGAGGGATGGAGTTCCTGGCTTCCCGAAAGTGCATCCACAGAGACCTGGCTGCTCGGAA CATTCTGCTGTCGGAAAGCGACGTGGTGAAGATCTGTGACTTTGGCCTTGCCCGGGACATCTACAAAGA CCCTGACTACGTCCGCAAGGGCAGTGCCCGGCTGCCCCTGAAGTGGATGGCCCCTGAAAGCATCTTCGA CAAGGTGTACACCACGCAGAGTGACGTGTGGTCCTTTGGGGTGCTTCTCTGGGAGATCTTCTCTCTGGG GGCCTCCCCGTACCCTGGGGTGCAGATCAATGAGGAGTTCTGCCAGCGGCTGAGAGACGGCACAAGGAT GAGGGCCCCGGAGCTGGCCACTCCCGCCATACGCCGCATCATGCTGAACTGCTGGTCCGGAGACCCCAA GGCGAGACCTGCATTCTCGGAGCTGGTGGAGATCCTGGGGGACCTGCTCCAGGGCAGGGGCCTGCAAGA GGAAGAGGAGGTCTGCATGGCCCCGCGCAGCTCTCAGAGCTCAGAAGAGGGCAGCTTCTCGCAGGTGTC CACCATGGCCCTACACATCGCCCAGGCTGACGCTGAGGACAGCCCGCCAAGCCTGCAGCGCCACAGCCT GGCCGCCAGGTATTACAACTGGGTGTCCTTTCCCGGGTGCCTGGCCAGAGGGGCTGAGACCCGTGGTTC CTCCAGGATGAAGACATTTGAGGAATTCCCCATGACCCCAACGACCTACAAAGGCTCTGTGGACAACCA GACAGACAGTGGGATGGTGCTGGCCTCGGAGGAGTTTGAGCAGATAGAGAGCAGGCATAGACAAGAAAG CGGCTTCAGGTAGCTGAAGCAGAGAGAGAGAAGGCAGCATACGTCAGCATTTTCTTCTCTGCACTTATA AGAAAGATCAAAGACTTTAAGACTTTCGCTATTTCTTCTACTGCTATCTACTACAAACTTCAAAGAGGA ACCAGGAGGACAAGAGGAGCATGAAAGTGGACAAGGAGTGTGACCACTGAAGCACCACAGGGAGGGGTT AGGCCTCCGGATGACTGCGGGCAGGCCTGGATAATATCCAGCCTCCCACAAGAAGCTGGTGGAGCAGAG TGTTCCCTGACTCCTCCAAGGAAAGGGAGACGCCCTTTCATGGTCTGCTGAGTAACAGGTGCCTTCCCA GACACTGGCGTTACTGCTTGACCAAAGAGCCCTCAAGCGGCCCTTATGCCAGCGTGACAGAGGGCTCAC CTCTTGCCTTCTAGGTCACTTCTCACAATGTCCCTTCAGCACCTGACCCTGTGCCCGCCAGTTATTCCT TGGTAATATGAGTAATACATCAAAGAGTAGTATTAAAAGCTAATTAATCATGTTTATACTAAAAAAAAA AAAAAAAAACTCGACTCTAGATTGCGGCCGC >OriGene 5' read for mutant NM_002020 unedited
ACCGCCGTCTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCATTTAGGTG ACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAATTCGGCACGAGGGTCGGACCCACG CGCAGCGGCCGGAGATGCAGCGGGGCGCCGCGCTGTGCCTGCGACTGTGGCTCTGCCTGGGACTCCTGGA CGGCCTGGTGAGTGGCTACTCCATGACCCCCCCGACCTTGAACATCACGGAGGAGTCACACGTCATCGAC ACCGGTGACAGCCTGTCCATCTCCTGCAGGGACAGCACCCCCCTCGAGTGGGCTTTGGCCAGGAAGCCTC CAGGGAGGGCGGCCAGCCCACCCGGAAGACACAAGGACAAGCCGAGGGACACGGGGGTGTGTGTGCCGAG ACTCTGCAGAGGCGCACCAGACACGCCCAGGGCCCTAACTGCAAAGGTGGTTGCTTGCTTGCACCAGAGG TACATGGCAACCCGACCCCAGGCGGCTCGTTTCTGCTACACACAGTTTACCTCAAGGGCCCCCATGCAGG GGAAAAAGGGGGGGAGGTTTTTAAGGTTTCGTAGAAAACATTTAGACCGCCCTTTTTTCAAAAACCTAAA CCCCCTTTGGTCAAAAGGGAAGGGCCCCGTGGGGGGGCCCGGGTTGGGTTGCCCCCCCGGCCCCCAAAGT TCCCCCCTCCCCCCCAAAACCCGGGCTCTTGGCCCAAAACCCCAAAATGTTTTTTAAAAAAACCGGGGCA TCCTCCTTTTTCCCCCCCCCGTGGCCAAAAACCCCTTGTTCCGGGGGGAAACCCTGGGGGGAAAAACAAT TTTTTTTTTACCCCTTTTTGGTGCGCATCACAAGGGAGAGAATTTTTTAAACCTCCCTTTTTTCCCCAAA AATTCTTGAGTCTCTGTGTTAGAGAAAACACGGCTCTCACATCTCGCCGGGCCGACTTATCATCACGTGT ACCCTTTAATGCGATACACCCAGGAACGAGCGCA >SC323508 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
ARRACGGCTGCCCTGGGGAGAGTGCTCGGCTACGGCGCCTTCGGGAAGGTGGTGGAAGCCTCCGCTTTCG GCATCCACAAGGGCAGCAGCTGTGACACCGTGGCCGTGATGATGCTGAAAGAGGGCGCCACGGCCAGCGA GCACCGCGCGCTGATGTCGGAGCTCAAGATCCTCATTCACATCGGCAACCACCTCAACGTGGTCAACCTC CTCGGGGCGTGCACCAAGCCGCAGGGCCCCCTCATGGTGATCGTG |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_002020 |
| Insert Size | 4516 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002020.1 |
| RefSeq Size | 4450 bp |
| RefSeq ORF | 4516 bp |
| Locus ID | 2324 |
| UniProt ID | P35916 |
| Domains | pkinase, TyrKc, S_TKc, ig, IGc2, IG |
| Protein Families | Druggable Genome, Protein Kinase, Transmembrane |
| Protein Pathways | Cytokine-cytokine receptor interaction, Focal adhesion |
| MW | 161.4 kDa |
| Gene Summary | This gene encodes a tyrosine kinase receptor for vascular endothelial growth factors C and D. The protein is thought to be involved in lymphangiogenesis and maintenance of the lymphatic endothelium. Mutations in this gene cause hereditary lymphedema type IA. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2) contains an alternate 3' terminal exon compared to variant 1. This results in an isoform (2) with a shorter C-terminus compared to isoform 1. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214232 | FLT4 (Myc-DDK-tagged)-Human fms-related tyrosine kinase 4 (FLT4), transcript variant 2 |
CNY 12,920.00 |
|
| RC214232L3 | Lenti-ORF clone of FLT4 (Myc-DDK-tagged)-Human fms-related tyrosine kinase 4 (FLT4), transcript variant 2 |
CNY 14,820.00 |
|
| RC214232L4 | Lenti-ORF clone of FLT4 (mGFP-tagged)-Human fms-related tyrosine kinase 4 (FLT4), transcript variant 2 |
CNY 14,820.00 |
|
| RC600029 | FLT4 (DDK-His-tagged)-Extra Cellular Domain Clone of Homo sapiens fms-related tyrosine kinase 4, transcript variant 2, Signal peptide (1-24) plus EC domain (25-775) |
CNY 8,650.00 |
|
| RG214232 | FLT4 (tGFP-tagged) - Human fms-related tyrosine kinase 4 (FLT4), transcript variant 2 |
CNY 14,250.00 |
|
| SC118894 | FLT4 (untagged)-Human fms-related tyrosine kinase 4 (FLT4), transcript variant 2 |
CNY 9,048.00 |
