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RIOK2 (NM_018343) Human Untagged Clone
CAT＃: SC323501
RIOK2 (untagged)-Kinase deficient mutant (D228A) of Human RIO kinase 2 (yeast) (RIOK2)
CNY 4,120.00
CNY 7,220.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | RIO2 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323501 sequence for NM_018343 edited (data generated by NextGen Sequencing)
ATGGGGAAAGTGAATGTGGCCAAGTTGCGTTACATGAGCCGAGATGACTTCAGGGTCTTG ACCGCGGTTGAAATGGGCATGAAGAACCATGAAATTGTTCCCGGCAGTTTGATTGCTTCT ATAGCCAGCCTTAAACATGGTGGCTGTAATAAAGTTTTAAGAGAATTAGTGAAACATAAA CTCATAGCTTGGGAGCGTACCAAAACTGTCCAGGGCTATCGGTTGACAAATGCAGGATAT GATTACCTAGCTTTGAAAACACTTTCTTCTAGGCAAGTAGTTGAGTCTGTTGGAAACCAG ATGGGTGTTGGCAAAGAATCAGATATTTACATTGTTGCAAATGAAGAAGGACAACAATTT GCATTAAAGCTTCACAGACTAGGAAGAACCTCGTTTCGAAATTTGAAAAACAAACGCGAT TATCATAAACATAGGCACAATGTGTCTTGGCTTTATTTATCTCGTCTCTCTGCCATGAAG GAATTTGCCTATATGAAGGCATTGTATGAGAGGAAATTTCCAGTTCCAAAGCCAATTGAT TACAATCGTCATGCAGTGGTCATGGAACTCATAAATGGTTATCCACTATGTCAGATACAC CATGTTGAAGATCCTGCATCAGTATATGATGAAGCTATGGAACTAATTGTCAAACTTGCA AATCATGGGCTGATTCATGGAGCKTTTAATGAATTTAATCTCATTTTGGATGAAAGTGAC CATATCACCATGATTGATTTTCCACAGATGGTTTCAACTTCTCATCCCAATGCTGAGTGG TATTTTGACAGAGATGTTAAATGCATTAAAGATTTCTTTATGAAACGTTTCAGCTACGAA AGTGAGCTTTTTCCAACTTTTAAGGATATCAGGAGAGAAGACACTCTTGATGTGGAGGTT TCTGCCAGTGGCTACACAAAGGAAATGCAGGCAGATGATGAACTGCTTCATCCATTAGGT CCAGATGATAAAAATATTGAAACAAAAGAGGGATCTGAATTCTCATTTTCAGATGGAGAA GTGGCAGAAAAAGCAGAGGTTTACAGGTCAGAAAATGAAAGTGAACGGAACTGTCTAGAA GAATCAGAGGGCTGCTATTGCAGATCATCTGGAGACCCTGAACAAATAAAGGAAGACAGT TTATCAGAAGAGAGTGCTGATGCACGGAGTTTTGAAATGACTGAATTCAATCAAGCTTTA GAAGAAATAAAAGGGCAGGTTGTTGAAAACAACTCTGTAACTGAATTTTCTGAGGAGAAA AACAGAACTGAAAATTACAACAGGCAAGATGGTCAGAGAGTTCAAGGAGGAGTCCCTGCT GGCTCTGACGAGTATGAAGATGAATGCCCTCATCTAATTGCCTTGTCGTCATTAAATAGA GAATTCAGGCCTTTCAGAGATGAAGAAAATGTGGGAGCTATGAATCAGTATAGAACAAGA ACTCTGAGTATCACTTCTTCAGGCAGTGCTGTAAGCTGTTCAACAATTCCTCCAGAACTG GTGAAACAGAAGGTGAAACGTCAGTTGACAAAACAGCAAAAATCAGCTGTCAGACGTCGA TTGCAGAAAGGAGAAGCAAATATATTTACCAAGCAACGTAGGGAAAACATGCAAAATATC AAATCAAGTTTGGAAGCAGCTAGCTTTTGGGGAGAATAA Clone variation with respect to NM_018343.2 447 a=>t;453 a=>t;683 a=>c;684 t=>k;1045 g=>a;1641 c=>t >OriGene 5' read for mutant NM_018343 unedited
CCCGCCCGTTCAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGA ACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTCGTCGGAGAGG CATCTGGGTTCGGACTGGGGCCGCCATGGGGAAAGTGAATGTGGCCAAGTTGCGTTACATGAGCCGAGAT GACTTCAGGGTCTTGACCGCGGTTGAAATGGGCATGAAGAACCATGAAATTGTTCCCGGCAGTTTGATTG CTTCTATAGCCAGCCTTAAACATGGTGGCTGTAATAAAGTTTTAAGAGAATTAGTGAAAACATAAACTCA TAGCTTGGGGAGCGTACCCAAAACTGTCCAGGGCTATCGGTTGACAAATGCAGGATATGATTACCTAGCT TTGAAAACCCTTTTCTTCTAGGCAAGTAGTTGAGTCCGGTGGAAACCCAGAGGGTGGTTGGCAAAGAATC AGATATTAAATGGTGCAAAGGAGAAGGGACAACATTTCCTTAAACCTCACACATAGAAAAACCCTGTTCC AATTTGAAAACACAACGCATATATAAACCTAGGCCATGTGGCTGGCTAATTATCTCTCATGGCCGAGAGA TTCCTATAAGCCTGTGAGAGAGAATCCGTTCAACGAAGTGATCACACACCACC >SC323501 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
GWCCCATGTTGAGWCCTGCATCAGTATATGATGAAGCTATGGAACTAATTGTCAAACTTGCAAATCATGG GCTGATTCATGGAGCGTTTAATGAATTTAATCTCATTTTGGATGAAAGTGACCATATCACCATGATTGAT TTTCCACAGATGGTTTCAACTTCTCATCCCAATGCTGAGTGGTATTTTGACAGAGATGTTAAATGCATTA AAGATTTCTTTATGAAACGTTTCAGCTACGAAAGTGAGCTTTTTC |
| Restriction Sites | Please inquire |
| ACCN | NM_018343 |
| Insert Size | 2110 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_018343.1, NP_060813.1 |
| RefSeq Size | 1832 bp |
| RefSeq ORF | 1659 bp |
| Locus ID | 55781 |
| UniProt ID | Q9BVS4 |
| Domains | RIO |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | Serine/threonine-protein kinase involved in the final steps of cytoplasmic maturation of the 40S ribosomal subunit. Involved in export of the 40S pre-ribosome particles (pre-40S) from the nucleus to the cytoplasm. Its kinase activity is required for the release of NOB1, PNO1 and LTV1 from the late pre-40S and the processing of 18S-E pre-rRNA to the mature 18S rRNA (PubMed:19564402). Regulates the timing of the metaphase-anaphase transition during mitotic progression, and its phosphorylation, most likely by PLK1, regulates this function (PubMed:21880710).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201484 | RIOK2 (Myc-DDK-tagged)-Human RIO kinase 2 (RIOK2), transcript variant 1 |
CNY 3,990.00 |
|
| RC201484L1 | Lenti ORF clone of Human RIO kinase 2 (RIOK2), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201484L2 | Lenti ORF clone of Human RIO kinase 2 (RIOK2), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC201484L3 | Lenti ORF clone of Human RIO kinase 2 (RIOK2), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201484L4 | Lenti ORF clone of Human RIO kinase 2 (RIOK2), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG201484 | RIOK2 (tGFP-tagged) - Human RIO kinase 2 (RIOK2), transcript variant 1 |
CNY 4,370.00 |
|
| SC113560 | RIOK2 (untagged)-Human RIO kinase 2 (RIOK2), transcript variant 1 |
CNY 2,950.00 |
