United States
Germany
Japan
United Kingdom
China
RIOK2 (NM_018343) Human Tagged ORF Clone
CAT＃: RG201484
- TrueORF®
RIOK2 (tGFP-tagged) - Human RIO kinase 2 (RIOK2), transcript variant 1
ORF Plasmid: DDK
"NM_018343" in other vectors (7)
Need custom modification / cloning service?
Get a free quote
CNY 4,370.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | RIO2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG201484 representing NM_018343
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGAAAGTGAATGTGGCCAAGTTGCGTTACATGAGCCGAGATGACTTCAGGGTCTTGACCGCGGTTG AAATGGGCATGAAGAACCATGAAATTGTTCCCGGCAGTTTGATTGCTTCTATAGCCAGCCTTAAACATGG TGGCTGTAATAAAGTTTTAAGAGAATTAGTGAAACATAAACTCATAGCTTGGGAGCGTACCAAAACTGTC CAGGGCTATCGGTTGACAAATGCAGGATATGATTACCTAGCTTTGAAAACACTTTCTTCTAGGCAAGTAG TTGAGTCTGTTGGAAACCAGATGGGTGTTGGCAAAGAATCAGATATTTACATTGTTGCAAATGAAGAAGG ACAACAATTTGCATTAAAGCTTCACAGACTAGGAAGAACCTCGTTTCGAAATTTGAAAAACAAACGCGAT TATCATAAATATAGGCACAATGTGTCATGGCTATATTTATCTCGTCTCTCTGCCATGAAGGAATTTGCCT ATATGAAGGCATTGTATGAGAGGAAATTTCCAGTTCCAAAGCCAATTGATTACAATCGTCATGCAGTGGT CATGGAACTCATAAATGGTTATCCACTATGTCAGATACACCATGTTGAAGATCCTGCATCAGTATATGAT GAAGCTATGGAACTAATTGTCAAACTTGCAAATCATGGGCTGATTCATGGAGATTTTAATGAATTTAATC TCATTTTGGATGAAAGTGACCATATCACCATGATTGATTTTCCACAGATGGTTTCAACTTCTCATCCCAA TGCTGAGTGGTATTTTGACAGAGATGTTAAATGCATTAAAGATTTCTTTATGAAACGTTTCAGCTACGAA AGTGAGCTTTTTCCAACTTTTAAGGATATCAGGAGAGAAGACACTCTTGATGTGGAGGTTTCTGCCAGTG GCTACACAAAGGAAATGCAGGCAGATGATGAACTGCTTCATCCATTAGGTCCAGATGATAAAAATATTGA AACAAAAGAGGGATCTGAATTCTCATTTTCAGATGGAGAAGTGGCAGAAAAAGCAGAGGTTTACGGGTCA GAAAATGAAAGTGAACGGAACTGTCTAGAAGAATCAGAGGGCTGCTATTGCAGATCATCTGGAGACCCTG AACAAATAAAGGAAGACAGTTTATCAGAAGAGAGTGCTGATGCACGGAGTTTTGAAATGACTGAATTCAA TCAAGCTTTAGAAGAAATAAAAGGGCAGGTTGTTGAAAACAACTCTGTAACTGAATTTTCTGAGGAGAAA AACAGAACTGAAAATTACAACAGGCAAGATGGTCAGAGAGTTCAAGGAGGAGTCCCTGCTGGCTCTGACG AGTATGAAGATGAATGCCCTCATCTAATTGCCTTGTCGTCATTAAATAGAGAATTCAGGCCTTTCAGAGA TGAAGAAAATGTGGGAGCTATGAATCAGTATAGAACAAGAACTCTGAGTATCACTTCTTCAGGCAGTGCT GTAAGCTGTTCAACAATTCCTCCAGAACTGGTGAAACAGAAGGTGAAACGTCAGTTGACAAAACAGCAAA AATCAGCTGTCAGACGTCGATTGCAGAAAGGAGAAGCAAATATATTTACCAAGCAACGTAGGGAAAACAT GCAAAATATCAAATCAAGTTTGGAAGCAGCCAGCTTTTGGGGAGAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG201484 representing NM_018343
Red=Cloning site Green=Tags(s) MGKVNVAKLRYMSRDDFRVLTAVEMGMKNHEIVPGSLIASIASLKHGGCNKVLRELVKHKLIAWERTKTV QGYRLTNAGYDYLALKTLSSRQVVESVGNQMGVGKESDIYIVANEEGQQFALKLHRLGRTSFRNLKNKRD YHKYRHNVSWLYLSRLSAMKEFAYMKALYERKFPVPKPIDYNRHAVVMELINGYPLCQIHHVEDPASVYD EAMELIVKLANHGLIHGDFNEFNLILDESDHITMIDFPQMVSTSHPNAEWYFDRDVKCIKDFFMKRFSYE SELFPTFKDIRREDTLDVEVSASGYTKEMQADDELLHPLGPDDKNIETKEGSEFSFSDGEVAEKAEVYGS ENESERNCLEESEGCYCRSSGDPEQIKEDSLSEESADARSFEMTEFNQALEEIKGQVVENNSVTEFSEEK NRTENYNRQDGQRVQGGVPAGSDEYEDECPHLIALSSLNREFRPFRDEENVGAMNQYRTRTLSITSSGSA VSCSTIPPELVKQKVKRQLTKQQKSAVRRRLQKGEANIFTKQRRENMQNIKSSLEAASFWGE TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_018343 |
| ORF Size | 1656 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_018343.1, NP_060813.1 |
| RefSeq Size | 1832 bp |
| RefSeq ORF | 1659 bp |
| Locus ID | 55781 |
| UniProt ID | Q9BVS4 |
| Domains | RIO |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | Serine/threonine-protein kinase involved in the final steps of cytoplasmic maturation of the 40S ribosomal subunit. Involved in export of the 40S pre-ribosome particles (pre-40S) from the nucleus to the cytoplasm. Its kinase activity is required for the release of NOB1, PNO1 and LTV1 from the late pre-40S and the processing of 18S-E pre-rRNA to the mature 18S rRNA (PubMed:19564402). Regulates the timing of the metaphase-anaphase transition during mitotic progression, and its phosphorylation, most likely by PLK1, regulates this function (PubMed:21880710).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201484 | RIOK2 (Myc-DDK-tagged)-Human RIO kinase 2 (RIOK2), transcript variant 1 |
CNY 3,990.00 |
|
| RC201484L1 | Lenti ORF clone of Human RIO kinase 2 (RIOK2), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201484L2 | Lenti ORF clone of Human RIO kinase 2 (RIOK2), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC201484L3 | Lenti ORF clone of Human RIO kinase 2 (RIOK2), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201484L4 | Lenti ORF clone of Human RIO kinase 2 (RIOK2), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| SC113560 | RIOK2 (untagged)-Human RIO kinase 2 (RIOK2), transcript variant 1 |
CNY 2,950.00 |
|
| SC323501 | RIOK2 (untagged)-Kinase deficient mutant (D228A) of Human RIO kinase 2 (yeast) (RIOK2) |
CNY 4,120.00 |
