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CGK2 (PRKG2) (NM_006259) Human Untagged Clone
CAT＃: SC323491
PRKG2 (untagged)-Kinase deficient mutant (K482M) of Human protein kinase, cGMP-dependent, type II (PRKG2)
CNY 5,592.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | cGK2; cGKII; PKG2; PRKGR2 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323491 sequence for NM_006259 edited (data generated by NextGen Sequencing)
ATGGGAAATGGTTCAGTGAAACCTAAACATTCTAAGCACCCAGATGGACACTCTGGGAAC CTCACCACTGATGCTCTGCGGAACAAGGTGACAGAGCTGGAGAGAGAGTTGAGGAGGAAG GATGCTGAGATCCAGGAGCGGGAGTACCATTTGAAGGAGCTGCGGGAGCAGCTGTCGAAG CAGACTGTGGCCATTGCTGAACTCACAGAGGAGCTCCAGAACAAGTGCATCCAGCTGAAC AAGCTGCAGGATGTGGTGCATATGCAGGGAGGAAGCCCGCTTCAGGCCTCTCCAGATAAA GTGCCTCTTGAGGTCCACCGGAAGACCTCTGGATTGGTCTCTCTCCATAGCAGGAGGGGA GCAAAGGCTGGCGTGTCTGCTGAGCCAACAACCCGGACCTATGACCTGAACAAACCCCCT GAATTTTCCTTTGAGAAAGCAAGAGTCAGAAAAGACTCCAGTGAGAAGAAGCTCATTACA GATGCCCTTAATAAAAATCAGTTTCTGAAAAGACTGGATCCTCAGCAGATCAAAGACATG GTGGAATGCATGTATGGGAGAAACTATCAGCAAGGGAGTTACATTATTAAGCAAGGAGAA CCAGGAAACCATATCTTTGTGCTGGCAGAGGGTCGACTAGAGGTGTTCCAAGGGGAGAAA TTGCTGTCCTCCATCCCTATGTGGACCACATTTGGGGAGCTTGCCATTTTATACAATTGT ACAAGGACTGCCTCTGTGAAAGCTATTACCAATGTTAAAACATGGGCACTAGATCGAGAG GTATTCCAGAATATAATGAGGAGGACAGCCCAAGCTAGAGATGAACAATACAGAAACTTC CTCAGAAGTGTATCCTTGCTGAAGAATTTACCTGAAGATAAATTAACCAAGATCATTGAC TGCTTGGAAGTGGAATACTATGACAAAGGAGATTACATCATTAGAGAGGGCGAGGAAGGA AGTACCTTTTTCATTTTGGCAAAAGGAAAGGTAAAAGTAACACAGAGCACAGAAGGCCAT GATCAACCACAGCTGATAAAAACACTGCAGAAAGGAGAATACTTTGGAGAAAAAGCTCTT ATCAGTGATGATGTCAGGTCAGCTAACATTATTGCTGAAGAAAATGATGTTGCATGCCTG GTTATAGATCGAGAAACATTCAACCAAACTGTCGGTACATTTGAAGAGCTGCAAAAATAC CTTGAAGGATATGTGGCAAACCTGAACCGTGATGATGAAAAAAGACATGCGAAGCGGTCC ATGTCTAACTGGAAGCTGTCCAAAGCACTCTCTCTGGAAATGATTCAGCTGAAGGAGAAG GTGGCCAGATTTTCCTCATCATCCCCATTCCAGAACCTTGAGATTATTGCAACACTGGGC GTTGGTGGGTTCGGAAGAGTTGAGCTTGTTAAAGTAAAAAATGAGAATGTTGCTTTTGCT ATGATGTGTATAAGGAAGAAGCACATAGTTGACACCAAGCAGCAGGAGCATGTCTACTCA GAGAAGAGGATCCTAGAGGAGCTGTGCTCTCCATTCATTGTGAAATTATATCGTACTTTC AAGGACAATAAGTATGTATACATGCTTCTGGAGGCCTGCTTAGGTGGGGAGCTCTGGAGT ATATTAAGGGACAGAGGCAGCTTTGATGAACCCACCTCCAAATTCTGCGTTGCTTGTGTG ACAGAAGCATTTGATTACCTGCATCGACTAGGTATTATCTACAGAGACTTGAAACCAGAA AACTTAATTCTAGATGCTGAGGGTTACCTTAAATTGGTTGACTTTGGATTTGCGAAGAAA ATAGGGTCTGGACAGAAAACATGGACATTCTGTGGGACTCCAGAATATGTAGCTCCTGAA GTCATTCTCAACAAGGGACATGACTTCAGTGTGGATTTCTGGTCACTGGGAATTCTAGTG TATGAGCTCCTAACGGGCAACCCACCCTTTTCTGGGGTTGACCAAATGATGACCTACAAT TTGATTCTCAAAGGAATTGAAAAAATGGATTTTCCCAGGAAGATAACACGACGACCTGAG GATTTGATTCGGAGGCTTTGCAGGCAAAATCCAACAGAAAGGCTGGGAAATCTGAAGAAT GGAATAAATGACATTAAGAAACACAGGTGGTTAAATGGTTTTAATTGGGAGGGACTGAAA GCACGGAGCCTTCCATCACCTTTGCAAAGAGAGCTCAAGGGACCCATAGATCACAGCTAC TTTGACAAATATCCTCCTGAAAAGGGAATGCCTCCAGATGAGCTATCAGGCTGGGATAAA GACTTCTGA Clone variation with respect to NM_006259.1 1445 a=>t >OriGene 5' read for mutant NM_006259 unedited
ACCGCCGTCTGAGCAACTGGGCGGTAGGCGCTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGT GAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGCCAGAGTGAACT ATGGTTTTTGGTTGCCTTCAAAGATAATTAGGTCCCTGAGCAAAATGGGAAATGGTTCAGTGAAACCTAA ACATTCTAAGCACCCAGATGGACACTCTGGGAACCTCACCACTGATGCTCTGCGGAACAAGGTGACAGAG CTGGAGAGAGAGTTGAGGAGGAAGGATGCTGAGATCCAGGAGCGGGAGTACCATTTTGAAGGAGCTGCGG GGAGCAGCTGTCGAAGCAGACTTGTGGGCCATTTGCTTGACCTCCACAGAAGGAGCTTCCAGAACCAAGG TGCATCCCAGCTGAACCAAGCCTGCAGGAAGTGTGGTTGCATATGCCAGGGGAGGGAGCCCCGGCTCAAG GCCCTTCTCCGAATAAAGTGCCCCTTTGGAGGTCACACCGAAGAGACTCCTGGTTTGGTTTCTCTCCATT GGCAAGAAAGGGGGAGAAAAGGTTGCCGTGCCTCGTGAGGCAAACAAACCGGAACCTAGGACCTGAAAAC CCCCTGAATTCCTTTGGAAACCGAGATTCAAAAGAGACTCATGAGAAGAACTCATATACGATGCCCTATA TAAATATAGTTTCGAAAAACTGGTTCCCACACATACAGACATGTGTGAATGCAGTGTATGGAAACACTTC CCAGGGTTCACTTTTAGCCAGGGAACCGGGAACATCTTGGCTGCAAGTCCACTAAGTGTTCCAAGGAGAT GCGCTCCATCCATTTGACCATGAGCTGCATTACATGCAAGGTCTGGACTCAGTACTGGCTATTCAAGATC GAATTAAGAAGAG >SC323491 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
TYATATCCCATTCAGACCTTGAGATTATTGCAACACTGGGCGTTGGTGGGTTCGGAAGAGTTGAGCTTGT TAAAGTAAAAAATGAGAATGTTGCTTTTGCTATGATGTGTATAAGGAAGAAGCACATAGTTGACACCAAG CAGCAGGAGCATGTCTACTCAGAGAAGAGGATCCTAGAGGAGCTGTGCTCTCCATTCATTGTGAAATTAT ATCGTACTTTCAAGGACAATAAGTATGTATACATGCTTCTGGAGG |
| Restriction Sites | Please inquire |
| ACCN | NM_006259 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_006259.1, NP_006250.1 |
| RefSeq Size | 3328 bp |
| RefSeq ORF | 2289 bp |
| Locus ID | 5593 |
| UniProt ID | Q13237 |
| Domains | cNMP, pkinase, S_TK_X, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Gap junction, Long-term depression, Olfactory transduction |
| Gene Summary | This gene encodes a protein that belongs to the serine/threonine protein kinase family of proteins. The encoded protein binds to and inhibits the activation of several receptor tyrosine kinases. The membrane-bound protein is a regulator of intestinal secretion, bone growth and renin secretion. Alternate splicing results in multiple transcript variants encoding distinct isoforms whose regulatory N-termini differ in length but whose C-terminal catalytic domains are identical. [provided by RefSeq, May 2018] Transcript Variant: This variant (1) encodes the longest isoform (a). Variants 1 and 7 encode the same isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC215879 | PRKG2 (Myc-DDK-tagged)-Human protein kinase, cGMP-dependent, type II (PRKG2) |
CNY 5,584.00 |
|
| RC215879L1 | Lenti ORF clone of Human protein kinase, cGMP-dependent, type II (PRKG2), Myc-DDK-tagged |
CNY 7,984.00 |
|
| RC215879L2 | Lenti ORF clone of Human protein kinase, cGMP-dependent, type II (PRKG2), mGFP tagged |
CNY 7,700.00 |
|
| RC215879L3 | Lenti ORF clone of Human protein kinase, cGMP-dependent, type II (PRKG2), Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC215879L4 | Lenti ORF clone of Human protein kinase, cGMP-dependent, type II (PRKG2), mGFP tagged |
CNY 7,984.00 |
|
| RG215879 | PRKG2 (tGFP-tagged) - Human protein kinase, cGMP-dependent, type II (PRKG2) |
CNY 7,184.00 |
|
| SC116254 | PRKG2 (untagged)-Human protein kinase, cGMP-dependent, type II (PRKG2) |
CNY 5,592.00 |
