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CAMK2B (NM_172078) Human Untagged Clone
CAT＃: SC323488
CAMK2B (untagged)-Kinase deficient mutant (K43M) of Human calcium/calmodulin-dependent protein kinase II beta (CAMK2B), transcript variant 2
CNY 4,040.00
CNY 8,650.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CAM2; CAMK2; CAMKB; CaMKIIbeta; MRD54 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323488 sequence for NM_172078 edited (data generated by NextGen Sequencing)
ATGGCCACCACGGTGACCTGCACCCGCTTCACCGACGAGTACCAGCTCTACGAGGATATT GGCAAGGGGGCTTTCTCTGTGGTCCGACGCTGTGTCAAGCTCTGCACCGGCCATGAGTAT GCAGCCAAGATCATCAACACCAAGAAGCTGTCAGCCAGAGATCACCAGAAGCTGGAGAGA GAGGCTCGGATCTGCCGCCTTCTGAAGCATTCCAACATCGTGCGTCTCCACGACAGCATC TCCGAGGAGGGCTTCCACTACCTGGTCTTCGATCTGGTCACTGGTGGGGAGCTCTTTGAA GACATTGTGGCGAGAGAGTACTACAGCGAGGCTGATGCCAGTCACTGTATCCAGCAGATC CTGGAGGCCGTTCTCCATTGTCACCAAATGGGGGTCGTCCACAGAGACCTCAAGCCGGAG AACCTGCTTCTGGCCAGCAAGTGCAAAGGGGCTGCAGTGAAGCTGGCAGACTTCGGCCTA GCTATCGAGGTGCAGGGGGACCAGCAGGCATGGTTTGGTTTCGCTGGCACACCAGGCTAC CTGTCCCCTGAGGTCCTTCGCAAAGAGGCGTATGGCAAGCCTGTGGACATCTGGGCATGT GGGGTGATCCTGTACATCCTGCTCGTGGGCTACCCACCCTTCTGGGACGAGGACCAGCAC AAGCTGTACCAGCAGATCAAGGCTGGTGCCTATGACTTCCCGTCCCCTGAGTGGGACACC GTCACTCCTGAAGCCAAAAACCTCATCAACCAGATGCTGACCATCAACCCTGCCAAGCGC ATCACAGCCCATGAGGCCCTGAAGCACCCGTGGGTCTGCCAACGCTCCACGGTAGCATCC ATGATGCACAGACAGGAGACTGTGGAGTGTCTGAAAAAGTTCAATGCCAGGAGAAAGCTC AAGGGAGCCATCCTCACCACCATGCTGGCCACACGGAATTTCTCAGTGGGCAGACAGACC ACCGCTCCGGCCACAATGTCCACCGCGGCCTCCGGCACCACCATGGGGCTGGTGGAACAA GCCAAGAGTTTACTCAACAAGAAAGCAGATGGAGTCAAGCCCCAGACGAATAGCACCAAA AACAGTGCAGCCGCCACCAGCCCCAAAGGGACGCTTCCTCCTGCCGCCCTGGAGCCTCAA ACCACCGTCATCCATAACCCAGTGGACGGGATTAAGGAGTCTTCTGACAGTGCCAATACC ACCATAGAGGATGAAGACGCTAAAGCCCGGAAGCAGGAGATCATTAAGACCACGGAGCAG CTCATCGAGGCCGTCAACAACGGTGACTTTGAGGCCTACGCGAAAATCTGTGACCCAGGG CTGACCTCGTTTGAGCCTGAAGCACTGGGCAACCTGGTTGAAGGGATGGACTTCCACAGA TTCTACTTCGAGAACCTGCTGGCCAAGAACAGCAAGCCGATCCACACGACCATCCTGAAC CCACACGTGCACGTCATTGGAGAGGATGCCGCCTGCATCGCTTACATCCGGCTCACGCAG TACATTGACGGGCAGGGCCGGCCCCGCACCAGCCAGTCTGAGGAGACCCGCGTGTGGCAC CGCCGCGACGGCAAGTGGCAGAACGTGCACTTCCACTGCTCGGGCGCGCCTGTGGCCCCG CTGCAGTGA Clone variation with respect to NM_172078.2 >OriGene 5' read for mutant NM_172078 unedited
CCCGCCGTCTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGA ACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGGGAGCCGGAGT CGCCGCCGCCCGAGCGCAGCCGAGCGCACGCCGAGCCCGTCCGCCGCCGCCATGGCCACCACGGTGACCT GCACCCGCTTCACCGACGAGTACCAGCTCTACGAGGATATTGGCAAGGGGGCTTTCTCTGTGGTCCGACG CTGTGTCAAGCTCTGCACCGGCCATGAGTATGCAGCCATGATCATCAACACCAAGAAGCTGTCAGCCAGA GATCACCAGAAGCTGGAGAGAGAGGCTCGGATCTGCCGCCTTCTGAAGCATTCCACATCGTGCGTCTCCA CGACAGCATCTCCGGAGGAGGGCTTCCACTACCTGGTCCTTCGATCTGTTCACTGGGTGGGGGAGCTTCT TTGAAGACTTGGTGGCGAAGAGAGTACTACAGCCGAGGCCTGGATGCAGTCACTTGTATCCAGCAGATCC CTGGGAGGGCCGTCCTCCTTGGCCCCAAATGGGGGTCGTCCCCAGAGACCTCAGCCCGGAAACCTGCTTC TGGCACGCAGTGCAAAGGGGCTGCATGGAGCTGGCAATTCGGCTAGCTATCAGTCCAGGGGACAGCGGCA TGTTGGTCTCTTGCACCGGCACTGTCCCTGGTCCTCCAAAGCTACGGACCTGGACCTGGCATGGGGATCT CGAACATCGTCTTGGCCACCACCATCTGACAAAAAAAAGCGAA >SC323488 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
CCCTGMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTC AGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGGGAGCCGGAGTCGCCGC CGCCCGAGCGCAGCCGAGCGCACGCCGAGCCCGTCCGCCGCCGCCATGGCCACCACGGTGACCTGCACCC GCTTCACCGACGAGTACCAGCTCTACGAGGATATTGGCAAGGGGG |
| Restriction Sites | Please inquire |
| ACCN | NM_172078 |
| Insert Size | 1920 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_172078.1, NP_742075.1 |
| RefSeq Size | 2066 bp |
| RefSeq ORF | 1629 bp |
| Locus ID | 816 |
| UniProt ID | Q13554 |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Calcium signaling pathway, ErbB signaling pathway, Glioma, GnRH signaling pathway, Long-term potentiation, Melanogenesis, Neurotrophin signaling pathway, Olfactory transduction, Oocyte meiosis, Wnt signaling pathway |
| Gene Summary | The product of this gene belongs to the serine/threonine protein kinase family and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells, the enzyme is composed of four different chains: alpha, beta, gamma, and delta. The product of this gene is a beta chain. It is possible that distinct isoforms of this chain have different cellular localizations and interact differently with calmodulin. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014] Transcript Variant: This variant (2) lacks an in-frame segment of the coding region, compared to variant 1. It encodes a shorter isoform (2) that is missing an internal segment compared to isoform 1. Variants 2 and 9 encode the same protein (isoform 2). Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC218145 | CAMK2B (Myc-DDK-tagged)-Human calcium/calmodulin-dependent protein kinase II beta (CAMK2B), transcript variant 2 |
CNY 4,040.00 |
|
| RC218145L1 | Lenti ORF clone of Human calcium/calmodulin-dependent protein kinase II beta (CAMK2B), transcript variant 2, Myc-DDK-tagged |
CNY 6,440.00 |
|
| RC218145L2 | Lenti ORF clone of Human calcium/calmodulin-dependent protein kinase II beta (CAMK2B), transcript variant 2, mGFP tagged |
CNY 6,460.00 |
|
| RC218145L3 | Lenti ORF clone of Human calcium/calmodulin-dependent protein kinase II beta (CAMK2B), transcript variant 2, Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC218145L4 | Lenti ORF clone of Human calcium/calmodulin-dependent protein kinase II beta (CAMK2B), transcript variant 2, mGFP tagged |
CNY 6,460.00 |
|
| RG218145 | CAMK2B (tGFP-tagged) - Human calcium/calmodulin-dependent protein kinase II beta (CAMK2B), transcript variant 2 |
CNY 5,640.00 |
|
| SC109004 | CAMK2B (untagged)-Human calcium/calmodulin-dependent protein kinase II beta (CAMK2B), transcript variant 2 |
CNY 4,040.00 |
