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DYRK3 (NM_003582) Human Untagged Clone
CAT＃: SC323461
DYRK3 (untagged)-Kinase deficient mutant (K238M) of Human dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 3 (DYRK3), transcript variant 1
CNY 6,184.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | DYRK5; hYAK3-2; RED; REDK |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_003582, the custom clone sequence may differ by one or more nucleotides
ATGGGAGGCACAGCTCGTGGGCCTGGGCGGAAGGATGCGGGGCCGCCTGGGGCCGGGCTCCCGCCCCAGC AGCGGAGGTTGGGGGATGGTGTCTATGACACCTTCATGATGATAGATGAAACCAAATGTCCCCCCTGTTC AAATGTACTCTGCAATCCTTCTGAACCACCTCCACCCAGAAGACTAAATATGACCACTGAGCAGTTTACA GGAGATCATACTCAGCACTTTTTGGATGGAGGTGAGATGAAGGTAGAACAGCTGTTTCAAGAATTTGGCA ACAGAAAATCCAATACTATTCAGTCAGATGGCATCAGTGACTCTGAAAAATGCTCTCCTACTGTTTCTCA GGGTAAAAGTTCAGATTGCTTGAATACAGTAAAATCCAACAGTTCATCCAAGGCACCCAAAGTGGTGCCT CTGACTCCAGAACAAGCCCTGAAGCAATATAAACACCACCTCACTGCCTATGAGAAACTGGAAATAATTA ATTATCCAGAAATTTACTTTGTAGGTCCAAATGCCAAGAAAAGACATGGAGTTATTGGTGGTCCCAATAA TGGAGGGTATGATGATGCAGATGGGGCCTATATTCATGTACCTCGAGACCATCTAGCTTATCGATATGAG GTGCTGAAAATTATTGGCAAGGGGAGTTTTGGGCAGGTGGCCAGGGTCTATGATCACAAACTTCGACAGT ACGTGGCCCTAAAAATGGTGCGCAATGAGAAGCGCTTTCATCGTCAAGCAGCTGAGGAGATCCGGATTTT GGAGCATCTTAAGAAACAGGATAAAACTGGTAGTATGAACGTTATCCACATGCTGGAAAGTTTCACATTC CGGAACCATGTTTGCATGGCCTTTGAATTGCTGAGCATAGACCTTTATGAGCTGATTAAAAAAAATAAGT TTCAGGGTTTTAGCGTCCAGTTGGTACGCAAGTTTGCCCAGTCCATCTTGCAATCTTTGGATGCCCTCCA CAAAAATAAGATTATTCACTGCGATCTGAAGCCAGAAAACATTCTCCTGAAACACCACGGGCGCAGTTCA ACCAAGGTCATTGACTTTGGGTCCAGCTGTTTCGAGTACCAGAAGCTCTACACATATATCCAGTCTCGGT TCTACAGAGCTCCAGAAATCATCTTAGGAAGCCGCTACAGCACACCAATTGACATATGGAGTTTTGGCTG CATCCTTGCAGAACTTTTAACAGGACAGCCTCTCTTCCCTGGAGAGGATGAAGGAGACCAGTTGGCCTGC ATGATGGAGCTTCTAGGGATGCCACCACCAAAACTTCTGGAGCAATCCAAACGTGCCAAGTACTTTATTA ATTCCAAGGGCATACCCCGCTACTGCTCTGTGACTACCCAGGCAGATGGGAGGGTTGTGCTTGTGGGGGG TCGCTCACGTAGGGGTAAAAAGCGGGGTCCCCCAGGCAGCAAAGACTGGGGGACAGCACTGAAAGGGTGT GATGACTACTTGTTTATAGAGTTCTTGAAAAGGTGTCTTCACTGGGACCCCTCTGCCCGCTTGACCCCAG CTCAAGCATTAAGACACCCTTGGATTAGCAAGTCTGTCCCCAGACCTCTCACCACCATAGACAAGGTGTC AGGGAAACGGGTAGTTAATCCTGCAAGTGCTTTCCAGGGATTGGGTTCTAAGCTGCCTCCAGTTGTTGGA ATAGCCAATAAGCTTAAAGCTAACTTAATGTCAGAAACCAATGGTAGTATACCCCTATGCAGTGTATTGC CAAAACTGATTAGCTAG >OriGene 5' read for mutant NM_003582 unedited
CCCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTATGACACCTTCA TGATGATAGATGAAACCAAATGTCCCCCCTGTTCAAATGTACTCTGCAATCCTTCTGAACCACCTCCACC CAGAAGACTAAATATGACCACTGAGCAGTTTACAGGAGATCATACTCAGCACTTTTTGGATGGAGGTGAG ATGAAGGTAGAACAGCTGTTTCAAGAATTTGGCAACAGAAAAATCCAATACTATTTCAGTCAGATGGGCA TCAGTGACTCTGAAAAAATGCTCCTCCTACTGTTTCTCAGGGTAAAAAGTTCAGAATTGCTTGAATTACA GTAAAATCCAACAGTTTCATCCCAAGGGCACCCCAAGTTGGGTGCCCTCGGAACTCCGGAACAACCCTTG GAGCCATTTTAACCACCAACCTCATTGCCAATAGAAAACTGGAATTAATTTATTTATCCAGAAATTTACT TTGTGGGTCCAAATGCCAGAAAAGACATGGGGAGTTATTGGGGTTCCAAATAGGGGGGGTTAAATGATGC CATGGGGGCCCAATTTCTAGTGACCCGAGACCCACTATGCTTAACGAAATGAAGGTTGCGCTGAAATATT TGGCAAGGGGAGATTTGGGCAGGTGGGGCCGGGCTATGATTATCAAACTTTACAGTACGTGGGGCCCATG ATAGTGCGGCACTGAGGAGAAGCCTTTATCTCTAGCGCTGAGAGGATCTGCATTTGTAGCACTTATAAGA AGAGAACGTGTAGTACGTGTTCACACGCTGCGAGATTACTACGCACACGTTGTCGGCGCGATAGTGCATA CATCTAAACGATTAACAATTCGTGTATCACGCTTAGAGCAATGAGCACACATCGCCGACTCGATGGCTTC ACGACATAAAGAT >SC323461 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
TRRGGKCTGAATTATTGGCAGGGGAGTTTTGGGCAGGTGGCCAGGGTCTATGATCACAAACTTCGACAGT ACGTGGCCCTAATGATGGTGCGCAATGAGAAGCGCTTTCATCGTCAAGCAGCTGAGGAGATCCGGATTTT GGAGCATCTTAAGAAACAGGATAAAACTGGTAGTATGAACGTTATCCACATGCTGGAAAGTTTCACATTC CGGAACCATGTTTGCATGGCCTTTGAATTGCTGAGCATAGACCTT |
| Restriction Sites | Please inquire |
| ACCN | NM_003582 |
| Insert Size | 2050 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_003582.1, NP_003573.1 |
| RefSeq Size | 2141 bp |
| RefSeq ORF | 1662 bp |
| Locus ID | 8444 |
| UniProt ID | O43781 |
| Domains | pkinase |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | This gene product belongs to the DYRK family of dual-specificity protein kinases that catalyze autophosphorylation on serine/threonine and tyrosine residues. The members of this family share structural similarity, however, differ in their substrate specificity, suggesting their involvement in different cellular functions. The encoded protein has been shown to autophosphorylate on tyrosine residue and catalyze phosphorylation of histones H3 and H2B in vitro. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) represents the shorter transcript, but encodes the longer isoform (a), also known as the RED-L isoform. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC224654 | DYRK3 (Myc-DDK-tagged)-Human dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 3 (DYRK3), transcript variant 1 |
CNY 6,568.00 |
|
| RC224654L3 | Lenti-ORF clone of DYRK3 (Myc-DDK-tagged)-Human dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 3 (DYRK3), transcript variant 1 |
CNY 6,460.00 |
|
| RC224654L4 | Lenti-ORF clone of DYRK3 (mGFP-tagged)-Human dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 3 (DYRK3), transcript variant 1 |
CNY 6,460.00 |
|
| RG224654 | DYRK3 (tGFP-tagged) - Human dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 3 (DYRK3), transcript variant 1 |
CNY 8,168.00 |
|
| SC117867 | DYRK3 (untagged)-Human dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 3 (DYRK3), transcript variant 1 |
CNY 6,184.00 |
|
| SC318010 | DYRK3 (untagged)-Human dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 3 (DYRK3), transcript variant 1 |
CNY 9,500.00 |
