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PKMYT1 (NM_004203) Human Untagged Clone
CAT＃: SC323456
PKMYT1 (untagged)-Kinase deficient mutant (K139M) of Human protein kinase, membrane associated tyrosine/threonine 1 (PKMYT1), transcript variant 1
CNY 3,656.00
CNY 7,980.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | MYT1; PPP1R126 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323456 sequence for NM_004203 edited (data generated by NextGen Sequencing)
ATGCTAGAACGGCCTCCTGCACTGGCCATGCCCATGCCCACGGAGGGCACCCCGCCACCT CTGAGTGGCACCCCCATCCCAGTCCCAGCCTACTTCCGCCACGCAGAACCTGGATTCTCC CTCAAGAGGCCCAGGGGGCTCAGCCGGAGCCTCCCACCTCCGCCCCCTGCCAAGGGCAGC ATTCCCATCAGCCGCCTCTTCCCTCCTCGGACCCCAGGCTGGCACCAGCTGCAGCCCCGG CGGGTGTCATTCCGGGGCGAGGCCTCAGAGACTCTGCAGAGCCCTGGGTATGACCCAAGC CGGCCAGAGTCCTTCTTCCAGCAGAGCTTCCAGAGGCTCAGCCGCCTGGGCCATGGCTCC TACGGAGAGGTCTTCAAGGTGCGCTCCAAGGAGGACGGCCGGCTCTATGCGGTAATGCGT TCCATGTCACCATTCCGGGGCCCCAAGGACCGGGCCCGCAAGTTGGCCGAGGTGGGCAGC CACGAGAAGGTGGGGCAGCACCCATGCTGCGTGCGGCTGGAGCAGGCCTGGGAGGAGGGC GGCATCCTGTACCTGCAGACGGAGCTGTGCGGGCCCAGCCTGCAGCAACACTGTGAGGCC TGGGGTGCCAGCCTGCCTGAGGCCCAGGTCTGGGGCTACCTGCGGGACACGCTGCTTGCC CTGGCCCATCTGCACAGCCAGGGCCTGGTGCACCTTGATGTCAAGCCTGCCAACATCTTC CTGGGGCCCCGGGGCCGCTGCAAGCTGGGTGACTTCGGACTGCTGGTGGAGCTGGGTACA GCAGGAGCTGGTGAGGTCCAGGAGGGAGACCCCCGCTACATGGCCCCCGAGCTGCTGCAG GGCTCCTATGGGACAGCAGCGGATGTGTTCAGTCTGGGCCTCACCATCCTGGAAGTGGCA TGCAACATGGAGCTGCCCCACGGTGGGGAGGGCTGGCAGCAGCTGCGCCAGGGCTACCTG CCCCCTGAGTTCACTGCCGGTCTGTCTTCCGAGCTGCGTTCTGTCCTTGTCATGATGCTG GAGCCAGACCCCAAGCTGCGGGCCACGGCCGAGGCCCTGCTGGCACTGCCTGTGTTGAGG CAGCCGCGGGCCTGGGGTGTGCTGTGGTGCATGGCAGCGGAGGCCCTGAGCCGAGGGTGG GCCCTGTGGCAGGCCCTGCTTGCCCTGCTCTGCTGGCTCTGGCATGGGCTGGCTCACCCT GCCAGCTGGCTACAGCCCCTGGGCCCGCCAGCCACCCCGCCTGGCTCACCACCCTGCAGT TTGCTCCTGGACAGCAGCCTCTCCAGCAACTGGGATGACGACAGCCTAGGGCCTTCACTC TCCCCTGAGGCTGTCCTGGCCCGGACTGTGGGGAGCACCTCCACCCCCCGGAGCAGGTGC ACACCCAGGGATGCCCTGGACCTAAGTGACATCAACTCAGAGCCTCCTCGGGGCTCCTTC CCCTCCTTTGAGCCTCGGAACCTCCTCAGCCTGTTTGAGGACACCCTAGACCCAACCTGA Clone variation with respect to NM_004203.4 416 a=>t >OriGene 5' read for mutant NM_004203 unedited
ACCGCCCGTATCAGCAACTGGGCGGTAGGCGCTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAG TGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGCGCGCGTT CACGGGCCGTTCCCCCTCACGGGAGTCCTCCGCCCGGGCGTCCGGAACAGTCGACGGCAGACTCCGGCCC GCTGAGCCACCCGAGGGGTCCCGTGGCCTCCGCGGACCCGGAATCTGGGCCCTCGCGGACCCGCGCCCCG CCCAGTCGCCCCAGGGCTTCCCCACACCCACGGAGTGAAGTCAGCCGCGGCCCTGCCTGGGAGGGAACTT ACCGTCTACCGGGAAAAGGTGGGCCAGCAGATGTGTCGGGCCTGGTGAAGAGGGTGAAGGCGAAGACGGC CCGATCGCCAAGGGCCCCGGAAGCTGCGGAGGTCACCCCGCCCTGCCCTAAGCTCAGGGACACCCTGAAT TCCTTGGAACCCCTGGTTCTTGCCTCCCCGTTCCCACCATTGAGGCTGTTGGGCCAGGCCAGTTATGCTA AACGGCCTCTGCTGGGCCATGCCATCCCCGAGGGGCCCCGCCCCCCTTAAGGGCACAATACCATTGCAAC TATTTTGCGCACCAAACGGGTTTCACCAAAGCCCAGGGCGTCC >SC323456 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
GSGRTCTTCTCAGCAGAGCTTCCAGAGGCTCAGCCGCCTGGGCCATGGCTCCTACGGAGAGGTCTTCAAG GTGCGCTCCAAGGAGGACGGCCGGCTCTATGCGGTAATGCGTTCCATGTCACCATTCCGGGGCCCCAAGG ACCGGGCCCGCAAGTTGGCCGAGGTGGGCAGCCACGAGAAGGTGGGGCAGCACCCATGCTGCGTGCGGCT GGAGCAGGCCTGGGAGGAGGGCGGCATCCTGTACCTGCAGACGGA |
| Restriction Sites | Please inquire |
| ACCN | NM_004203 |
| Insert Size | 2190 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_004203.3, NP_004194.3 |
| RefSeq Size | 2121 bp |
| RefSeq ORF | 1500 bp |
| Locus ID | 9088 |
| UniProt ID | Q99640 |
| Domains | pkinase, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation |
| Gene Summary | This gene encodes a member of the serine/threonine protein kinase family. The encoded protein is a membrane-associated kinase that negatively regulates the G2/M transition of the cell cycle by phosphorylating and inactivating cyclin-dependent kinase 1. The activity of the encoded protein is regulated by polo-like kinase 1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012] Transcript Variant: This variant (1) encodes the longest isoform (1). |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222657 | PKMYT1 (Myc-DDK-tagged)-Human protein kinase, membrane associated tyrosine/threonine 1 (PKMYT1), transcript variant 1 |
CNY 3,656.00 |
|
| RC222657L1 | Lenti ORF clone of Human protein kinase, membrane associated tyrosine/threonine 1 (PKMYT1), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC222657L2 | Lenti ORF clone of Human protein kinase, membrane associated tyrosine/threonine 1 (PKMYT1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC222657L3 | Lenti ORF clone of Human protein kinase, membrane associated tyrosine/threonine 1 (PKMYT1), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC222657L4 | Lenti ORF clone of Human protein kinase, membrane associated tyrosine/threonine 1 (PKMYT1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG222657 | PKMYT1 (tGFP-tagged) - Human protein kinase, membrane associated tyrosine/threonine 1 (PKMYT1), transcript variant 1 |
CNY 5,256.00 |
|
| SC117519 | PKMYT1 (untagged)-Human protein kinase, membrane associated tyrosine/threonine 1 (PKMYT1), transcript variant 1 |
CNY 3,656.00 |
