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PIM2 (NM_006875) Human Untagged Clone
CAT＃: SC323418
PIM2 (untagged)-Kinase deficient mutant (K61M) of Human pim-2 oncogene (PIM2)
CNY 3,600.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323418 sequence for NM_006875 edited (data generated by NextGen Sequencing)
ATGTTGACCAAGCCTCTACAGGGGCCTCCCGCGCCCCCCGGGACCCCCACGCCGCCGCCA GGAGGCAAGGATCGGGAAGCGTTCGAGGCCGAGTATCGACTCGGCCCCCTCCTGGGTAAG GGGGGCTTTGGCACCGTCTTCGCAGGACACCGCCTCACAGATCGACTCCAGGTGGCCATC AYGGTGATTCCCCGGAATCGTGTGCTGGGCTGGTCCCCCTTGTCAGACTCAGTCACATGC CCACTCGAAGTCGCACTGCTATGGAAAGTGGGTGCAGGTGGTGGGCACCCTGGCGTGATC CGCCTGCTTGACTGGTTTGAGACACAGGAGGGCTTCATGCTGGTCCTCGAGCGGCCTTTG CCCGCCCAGGATCTCTTTGACTATATCACAGAGAAGGGCCCACTGGGTGAAGGCCCAAGC CGCTGCTTCTTTGGCCAAGTAGTGGCAGCCATCCAGCACTGCCATTCCCGTGGAGTTGTC CATCGTGACATCAAGGATGAGAACATCCTGATAGACCTACGCCGTGGCTGTGCCAAACTC ATTGATTTTGGTTCTGGTGCCCTGCTTCATGATGAACCCTACACTGACTTTGATGGGACA AGGGTGTACAGCCCCCCAGAGTGGATCTCTCGACACCAGTACCATGCACTCCCGGCCACT GTCTGGTCACTGGGCATCCTCCTCTATGACATGGTGTGTGGGGACATTCCCTTTGAGAGG GACCAGGAGATTCTGGAAGCTGAGCTCCACTTCCCAGCCCATGTCTCCCCAGACTGCTGT GCCCTAATCCGCCGGTGCCTGGCCCCCAAACCTTCTTCCCGACCCTCACTGGAAGAGATC CTGCTGGACCCCTGGATGCAAACACCAGCCGAGGATGTACCCCTCAACCCCTCCAAAGGA GGCCCTGCCCCTTTGGCCTGGTCCTTGCTACCCTAA Clone variation with respect to NM_006875.3 182 a=>y;183 a=>g >OriGene 5' read for mutant NM_006875 unedited
ACCGCCGTTCAGCAATGGGCGGTAGGCGTGTACGGCGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGATGTTGACCAAGCCT CTACAGGGGCCTCCCGCGCCCCCCGGGACCCCCACGCCGCCGCCAGGAGGCAAGGATCGGGAAGCGTTCG AGGCCGAGTATCGACTCGGCCCCCTCCTGGGTAAGGGGGGCTTTGGCACCGTCTTCGCAGGACACCGCCT CACAGATCGACTCCAGGTGGCCATCATGGTGATTCCCCGGAATCGTGTGCTGGGCTGGTCGCTGCTTTGT GTGTCTCAGTCACATGCCCACTCGAAGTCGCACTGCTATGGAAAGTGGGTGCAGGTGGTGGGCACCCTGC GTGATCCGCCTGCTTGACTGTTTGAGACACAGGAGGCTTCATGCTGGTCCTCGAGCGCCTTTGCCCGCCC AGGATTCTCTTTGACTTATATCCACAGAGAAGCCCACTGGGTGAAGCCCAAGCCGCTGCTTCTTGCAAGT ATGGCAGCATCAGCACTGCATTCCATGAAGTTGTCATCGTGACTCAAGATGAAAACCATCTTGATAGACC TACGGCTGGGCTGTGCAAATCTTATTGGATTTGTTTCTGGTGCTCTGCTTCGTGATGAACCTTACCTTGA CTTGAGTGGCACAGGGTGTCACGGCCCCAATGTGTATTCTTGGACACAGATACATAGTCATCCGGACAGT TGTGATATTGACTCCTTAAGAGTGTGTGGGACATTCCTTTAGAGCACAAGAATTCGAGAATCAGTATCTT ATCTAGCACACAGTGTCCAATGCTTGTGCCTATACGGTGCTGCGCGCCCAACTTCGACACTGGAATCGTG TGCAACCGTATTACACCC >SC323418 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
CSACTGMGCATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTC AGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGATGTTGACCAAGCCTCTACA GGGGCCTCCCGCGCCCCCCGGGACCCCCACGCCGCCGCCAGGAGGCAAGGATCGGGAAGCGTTCGAGGCC GAGTATCGACTCGGCCCCCTCCTGGGTAAGGGGGGCTTTGGCACC |
| Restriction Sites | Please inquire |
| ACCN | NM_006875 |
| Insert Size | 2000 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_006875.2, NP_006866.2 |
| RefSeq Size | 2101 bp |
| RefSeq ORF | 936 bp |
| Locus ID | 11040 |
| UniProt ID | Q9P1W9 |
| Domains | pkinase, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Acute myeloid leukemia |
| Gene Summary | This gene encodes a protooncogene that acts as a serine/threonine protein kinase. Studies determined the encoded protein functions to prevent apoptosis and to promote cell survival.[provided by RefSeq, Nov 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201933 | PIM2 (Myc-DDK-tagged)-Human pim-2 oncogene (PIM2) |
CNY 3,600.00 |
|
| RC201933L1 | Lenti ORF clone of Human pim-2 oncogene (PIM2), Myc-DDK-tagged |
CNY 6,000.00 |
|
| RC201933L2 | Lenti ORF clone of Human pim-2 oncogene (PIM2), mGFP tagged |
CNY 6,000.00 |
|
| RC201933L3 | Lenti ORF clone of Human pim-2 oncogene (PIM2), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201933L4 | Lenti ORF clone of Human pim-2 oncogene (PIM2), mGFP tagged |
CNY 6,000.00 |
|
| RG201933 | PIM2 (tGFP-tagged) - Human pim-2 oncogene (PIM2) |
CNY 5,200.00 |
|
| SC115825 | PIM2 (untagged)-Human pim-2 oncogene (PIM2) |
CNY 3,600.00 |
