United States
Germany
Japan
United Kingdom
China
ERK5 (MAPK7) (NM_139033) Human Untagged Clone
CAT＃: SC323406
MAPK7 (untagged)-Kinase deficient mutant (K84M) of Human mitogen-activated protein kinase 7 (MAPK7), transcript variant 1
CNY 15,680.00
Product images
CNY 1,999.00
CNY 2,700.00
热销推荐Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | BMK1; ERK4; ERK5; PRKM7 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_139033, the custom clone sequence may differ by one or more nucleotides
ATGGCCGAGCCTCTGAAGGAGGAAGACGGCGAGGACGGCTCTGCGGAGCCCCCCGGGCCCGTGAAGGCCG AACCCGCCCACACCGCTGCCTCTGTAGCGGCCAAGAACCTGGCCCTGCTTAAAGCCCGCTCCTTCGATGT GACCTTTGACGTGGGCGACGAGTACGAGATCATCGAGACCATAGGCAACGGGGCCTATGGAGTGGTGTCC TCCGCCCGCCGCCGCCTCACCGGCCAGCAGGTGGCCATCAAGAAGATCCCTAATGCTTTCGATGTGGTGA CCAATGCCAAGCGGACCCTCAGGGAGCTGAAGATCCTCAAGCACTTTAAACACGACAACATCATCGCCAT CAAGGACATCCTGAGGCCCACCGTGCCCTATGGCGAATTCAAATCTGTCTACGTGGTCCTGGACCTGATG GAAAGCGACCTGCACCAGATCATCCACTCCTCACAGCCCCTCACACTGGAACACGTGCGCTACTTCCTGT ACCAACTGCTGCGGGGCCTGAAGTACATGCACTCGGCTCAGGTCATCCACCGTGACCTGAAGCCCTCCAA CCTATTGGTGAATGAGAACTGTGAGCTCAAGATTGGTGACTTTGGTATGGCTCGTGGCCTGTGCACCTCG CCCGCTGAACATCAGTACTTCATGACTGAGTATGTGGCCACGCGCTGGTACCGTGCGCCCGAGCTCATGC TCTCTTTGCATGAGTATACACAGGCTATTGACCTCTGGTCTGTGGGCTGCATCTTTGGTGAGATGCTGGC CCGGCGCCAGCTCTTCCCAGGCAAAAACTATGTACACCAGCTACAGCTCATCATGATGGTGCTGGGTACC CCATCACCAGCCGTGATTCAGGCTGTGGGGGCTGAGAGGGTGCGGGCCTATATCCAGAGCTTGCCACCAC GCCAGCCTGTGCCCTGGGAGACAGTGTACCCAGGTGCCGACCGCCAGGCCCTATCACTGCTGGGTCGCAT GCTGCGTTTTGAGCCCAGCGCTCGCATCTCAGCAGCTGCTGCCCTTCGCCACCCTTTCCTGGCCAAGTAC CATGATCCTGATGATGAGCCTGACTGTGCCCCGCCCTTTGACTTTGCCTTTGACCGCGAAGCCCTCACTC GGGAGCGCATTAAGGAGGCCATTGTGGCTGAAATTGAGGACTTCCATGCAAGGCGTGAGGGCATCCGCCA ACAGATCCGCTTCCAGCCTTCTCTACAGCCTGTGGCTAGTGAGCCTGGCTGTCCAGATGTTGAAATGCCC AGTCCCTGGGCTCCCAGTGGGGACTGTGCCATGGAGTCTCCACCACCAGCCCCGCCACCATGCCCCGGCC CTGCACCTGACACCATTGATCTGACCCTGCAGCCACCTCCACCAGTCAGTGAGCCTGCCCCACCAAAGAA AGATGGTGCCATCTCAGACAATACTAAGGCTGCCCTTAAAGCTGCCCTGCTCAAGTCTTTGAGGAGCCGG CTCAGAGATGGCCCCAGCGCACCCCTGGAGGCTCCTGAGCCTCGGAAGCCGGTGACAGCCCAGGAGCGCC AGCGGGAGCGGGAGGAGAAGCGGCGGAGGCGGCAAGAACGAGCCAAGGAGCGGGAGAAACGGCGGCAGGA GCGGGAGCGAAAGGAACGGGGGGCTGGGGCCTCTGGGGGCCCCTCCACTGACCCCTTGGCTGGACTAGTG CTCAGTGACAATGACAGAAGCCTGTTGGAACGCTGGACTCGAATGGCCCGGCCCGCAGCCCCAGCCCTCA CCTCTGTGCCGGCCCCTGCCCCAGCGCCAACGCCAACCCCAACCCCAGTCCAACCTACCAGTCCTCCTCC TGGCCCTGTAGCCCAGCCCACTGGCCCGCAACCACAATCTGCGGGCTCTACCTCTGGCCCTGTACCCCAG CCTGCCTGCCCACCCCCTGGCCCTGCACCCCACCCCACTGGCCCTCCTGGGCCCATCCCTGTCCCCGCGC CACCCCAGATTGCCACCTCCACCAGCCTCCTGGCTGCCCAGTCACTTGTGCCACCCCCTGGGCTGCCTGG CTCCAGCACCCCAGGAGTTTTGCCTTACTTCCCACCTGGCCTGCCGCCCCCAGACGCCGGGGGAGCCCCT CAGTCTTCCATGTCAGAGTCACCTGATGTCAACCTTGTGACCCAGCAGCTATCTAAGTCACAGGTGGAGG ACCCCCTGCCCCCTGTGTTCTCAGGCACACCAAAGGGCAGTGGGGCTGGCTACGGTGTTGGCTTTGACCT GGAGGAATTCTTAAACCAGTCTTTCGACATGGGCGTGGCTGATGGGCCACAGGATGGCCAGGCAGATTCA GCCTCTCTCTCAGCCTCCCTGCTTGCTGACTGGCTCGAAGGCCATGGCATGAACCCTGCCGATATTGAGT CCCTGCAGCGTGAGATCCAGATGGACTCCCCAATGCTGCTGGCTGACCTGCCTGACCTCCAGGACCCCTG A >OriGene 5' read for mutant NM_139033 unedited
ACCGCCCGTCCAGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGA ACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGCGGAGACCCCCGCG CTGGGGACGGGAGGCCGGCGAGCCTCGGGACCTCTGAAAGCCTTGAGGAGGCGCGGGGACACCATGGCCG AGCCTCTGAAGGAGGAAGACGGCGAGGACGGCTCTGCGGAGCCCCCCGGGCCCGTGAAGGCCGAACCCGC CCACACCGCTGCCTCTGTAGCGGCCAAGAACCTGGCCCTGCTTAAAGCCCGCTCCTTCGATGTGGCGCTT GTGATCGTGGGCGACGAGTACGAGATCATCGAGACCATAGGCAACGGGGCCTATGGAGTGGGTGTGGGGG GGGCGGCGGCGGCTTAACGGGCGCGCCAGGGTGGCCTCCTGGAAAATCCTTAAGGCTTTGAATGGGTTGA CAAAGGCCAACCGACCCCCTAGGGACCGAAGAATCCCAAGGCCCTTAAAAAGAAAAAATTATTGGAATTA GGGGACTCTGGGGGCACCCTGGCCTATGGCGCAATTCAAATCTGTTTACGTGGTCCGGGCCCGGAGGAAA GCGCCCTGCCCCATTATATCCTTCTCAAAAGCCTTTACAAGTGAAACGTTTGCTCATTTTGTTCACAATG TGGCGGGGGGCTCAAATTTAGCCCTTCGCAAGGGTCACCCGGGACCTGGCCCCTCCACTTTGTGTAGAAG AACATGGCGCCCAATGTGAAATTTTGGGGGGTGGGCGTGACCCTCCCCTGAGAACATACTTCTTATGAGT ATTGTGCGCCCCTGTGAGTGCGCGACACTGATCTCTTTGTGATAGTATCCACGAGTATGCTTCTTGTCGT GCGCAATCTTTGAAGATGCGCGCCGCCCGTCGTCGACAACTATATAGC >SC323406 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
CGACGMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTC AGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGCGGAGACCCCCGCGCTGGGG ACGGGAGGCCGGCGAGCCTCGGGACCTCTGAAAGCCTTGAGGAGGCGCGGGGACACCATGGCCGAGCCTC TGAAGGAGGAAGACGGCGAGGACGGCTCTGCGGAGCCCCCCGGGC |
| Restriction Sites | Please inquire |
| ACCN | NM_139033 |
| Insert Size | 3000 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_139033.1, NP_620602.1 |
| RefSeq Size | 3113 bp |
| RefSeq ORF | 2451 bp |
| Locus ID | 5598 |
| UniProt ID | Q13164 |
| Domains | pkinase, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Gap junction, GnRH signaling pathway, MAPK signaling pathway, Neurotrophin signaling pathway |
| Gene Summary | The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is specifically activated by mitogen-activated protein kinase kinase 5 (MAP2K5/MEK5). It is involved in the downstream signaling processes of various receptor molecules including receptor type kinases, and G protein-coupled receptors. In response to extracelluar signals, this kinase translocates to cell nucleus, where it regulates gene expression by phosphorylating, and activating different transcription factors. Four alternatively spliced transcript variants of this gene encoding two distinct isoforms have been reported. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) is also known as BMK1 alpha. It encodes a longer isoform (1), as compared to variant 2. Variants 1, 3 and 4 encode the same isoform. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC212228 | MAPK7 (Myc-DDK-tagged)-Human mitogen-activated protein kinase 7 (MAPK7), transcript variant 1 |
CNY 8,960.00 |
|
| RC212228L3 | Lenti-ORF clone of MAPK7 (Myc-DDK-tagged)-Human mitogen-activated protein kinase 7 (MAPK7), transcript variant 1 |
CNY 5,890.00 |
|
| RC212228L4 | Lenti-ORF clone of MAPK7 (mGFP-tagged)-Human mitogen-activated protein kinase 7 (MAPK7), transcript variant 1 |
CNY 5,890.00 |
|
| RG212228 | MAPK7 (tGFP-tagged) - Human mitogen-activated protein kinase 7 (MAPK7), transcript variant 1 |
CNY 4,370.00 |
|
| SC120584 | MAPK7 (untagged)-Human mitogen-activated protein kinase 7 (MAPK7), transcript variant 1 |
CNY 8,976.00 |
