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Snf1lk2 (SIK2) (NM_015191) Human Untagged Clone
CAT＃: SC323400
SIK2 (untagged)-Kinase deficient mutant (K49M) of Human salt-inducible kinase 2 (SIK2)
CNY 6,792.00
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | LOH11CR1I; QIK; SIK-2; SNF1LK2 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323400 sequence for NM_015191 edited (data generated by NextGen Sequencing)
ATGGTCATGGCGGATGGCCCGAGGCACTTGCAGCGCGGGCCGGTCCGGGTGGGGTTCTAC GACATCGAGGGCACGCTGGGCAAGGGCAACTTCGCTGTGGTGAAGCTGGGGCGGCACCGG ATCACCAAGACGGAGGTGGCAATAATGATAATCGATAAGTCTCAGCTGGATGCAGTGAAC CTTGAGAAAATCTACCGAGAAGTACAAATAATGAAAATGTTAGACCACCCTCACATAATC AAACTTTATCAGGTAATGGAGACCAAAAGTATGTTGTACCTTGTGACAGAATATGCCAAA AATGGAGAAATTTTTGACTATCTTGCTAATCATGGCCGGTTAAATGAGTCTGAAGCCAGG CGAAAATTCTGGCAAATCCTGTCTGCTGTTGATTATTGTCATGGTCGGAAGATTGTGCAC CGTGACCTCAAAGCTGAAAATCTCCTGCTGGATAACAACATGAATATCAAAATAGCAGAT TTCGGTTTTGGAAATTTCTTTAAAAGTGGTGAACTGCTGGCAACATGGTGTGGCAGCCCC CCTTATGCAGCCCCAGAAGTCTTTGAAGGGCAGCAGTATGAAGGACCACAGCTGGACATC TGGAGTATGGGAGTTGTTCTTTATGTCCTTGTCTGTGGAGCTCTGCCCTTTGATGGACCG ACTCTTCCAATTTTGAGGCAGAGGGTTCTGGAAGGAAGATTCCGGATTCCGTATTTCATG TCAGAAGATTGCGAGCACCTTATCCGAAGGATGTTGGTCCTAGACCCATCCAAACGGCTA ACCATAGCCCAAATCAAGGAGCATAAATGGATGCTCATAGAAGTTCCTGTCCAGAGACCT GTTCTCTATCCACAAGAGCAAGAAAATGAGCCATCCATCGGGGAGTTTAATGAGCAGGTT CTGCGACTGATGCACAGCCTTGGAATAGATCAGCAGAAAACCATTGAGTCTTTGCAGAAC AAGAGCTATAACCACTTTGCTGCCATTTATTTCTTGTTGGTGGAGCGCCTGAAATCACAT CGGAGCAGTTTCCCAGTGGAGCAGAGACTTGATGGCCGCCAGCGTCGGCCTAGCACCATT GCTGAGCAAACAGTTGCCAAGGCACAGACTGTGGGGCTCCCAGTGACCATGCATTCACCG AACATGAGGCTGCTGCGATCTGCCCTCCTCCCCCAGGCATCCAACGTGGAGGCCTTTTCA TTTCCAGCATCTGGCTGTCAGGCGGAAGCTGCATTCATGGAAGAAGAGTGTGTGGACACT CCAAAGGTCAATGGCTGTCTGCTTGACCCTGTGCCTCCTGTCCTGGTGCGGAAGGGATGC CAGTCACTGCCCAGCAACATGATGGAGACCTCCATTGACGAAGGGCTGGAGACAGAAGGA GAGGCCGAGGAAGACCCCGCTCATGCCTTTGAGGCATTTCAGTCCACACGCAGCGGGCAG AGACGGCACACTCTGTCAGAAGTGACCAATCAACTGGTCGTGATGCCTGGGGCAGGGAAA ATTTTCTCCATGAATGACAGCCCCTCCCTTGACAGTGTGGACTCTGAGTATGATATGGGG TCTGTTCAGAGGGACCTGAACTTTCTGGAAGACAACCCTTCCCTTAAGGACATCATGTTA GCCAATCAGCCTTCACCCCGCATGACATCTCCCTTCATAAGCCTGAGACCTACCAACCCA GCCATGCAGGCTCTGAGCTCCCAGAAACGAGAGGTCCACAACAGGTCTCCAGTGAGCTTC AGAGAGGGCCGCAGAGCATCAGATACCTCCCTCACCCAGGGAATTGTAGCATTTAGACAA CATCTTCAGAATCTGGCTAGAACCAAAGGAATTCTAGAGTTGAACAAAGTGCAGTTGTTG TATGAACAAATAGGACCGGAGGCAGACCCTAACCTGGCGCCGGCGGCTCCTCAGCTCCAG GACCTTGCTAGCAGCTGCCCTCAGGAAGAAGTTTCTCAGCAGCAGGAAAGCGTCTCCACT CTCCCTGCCAGCGTGCATCCCCAGCTGTCCCCACGGCAGAGCCTGGAGACCCAGTACCTG CAGCACAGACTCCAGAAGCCCAGCCTTCTGTCAAAGGCCCAGAACACCTGTCAGCTTTAT TGCAAAGAACCACCGCGGAGCCTTGAGCAGCAGCTGCAGGAACATAGGCTCCAGCAGAAG CGACTCTTTCTTCAGAAGCAGTCTCAACTGCAGGCCTATTTTAATCAGATGCAGATAGCA GAGAGCTCCTACCCACAGCCAAGTCAGCAGCTGCCCCTTCCCCGCCAGGAGACTCCACCG CCTTCTCAGCAGGCCCCACCGTTCAGCCTGACCCAGCCCCTGAGCCCCGTCCTGGAGCCT TCCTCCGAGCAGATGCAATACAGCCCTTTCCTCAGCCAGTACCAAGAGATGCAGCTTCAG CCCCTGCCCTCCACTTCCGGTCCCCGGGCTGCTCCTCCTCTGCCCACGCAGCTACAGCAG CAGCAGCCGCCACCGCCACCACCCCCTCCACCACCACGACAGCCAGGAGCTGCCCCAGCC CCCTTACAGTTCTCCTATCAGACTTGTGAGCTGCCAAGCGCTGCTTCCCCTGCGCCAGAC TATCCCACTCCCTGTCAGTATCCTGTGGATGGAGCCCAGCAGAGCGACCTAACGGGGCCA GACTGTCCCAGAAGCCCAGGACTGCAAGAGGCCCCCTCCAGCTACGACCCACTAGCCCTC TCTGAGCTACCTGGACTCTTTGATTGTGAAATGCTAGACGCTGTGGATCCACAACACAAC GGGTATGTCCTGGTGAATTAG Clone variation with respect to NM_015191.1 146 a=>t;147 a=>g >OriGene 5' read for mutant NM_015191 unedited
CCCGCCGTTGTGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGGCGGCGCGCGCC TGGCTGGGCCCTGCGGAGCGGGAGGGAAGGAGCGAAGGAGCGAAGGAGCAAGCGGAGCGGCCGTCGCCCA AGCCAAGCCGCGCTGCCAACCCTCCCGCCCGCCCGCGCTCCTGTCCGCCGTGTCTAGCAGCGGGGCCCAG CATGGTCATGGCGGATGGCCCGAGGCACTTGCAGCGCGGGCCGGTCCGGGGTGGGGGTTCTACGACATCC GAGGGGCACGCTTGGGCCAAGGGCAACTTCGCTGTGGTGGAAGCTGGGGCCGGGCACCCGAATCACCAAG AACGGAAGGTGGGCAATAAGGATAATCATTAATTTCTAAGCTTGGATGCAGTGGAACCTGGGAAAAAACC TACCGAAAAGACACAAATAAGGAAAAGTGTAAAACCCCCCCCTCATATATCAAATTTTTTCGGGAAGGGG AAACCAAAGTTTTGGTTATCCCTTTGTGACGAAATTGCCCAAAAGGGGAAAATTTTTACAATACTGGGAA ACCGGGCCGTTAAAAAGACTCAAAACCCAGGAAAAATTTGGGAAAACCCGGTCGGGTGAAATTGGGCCGG GGGCAAAAAAATGGCCCCGGGACCCAAACTAAAAAATTCCGGGGGGATAACCACGGGAATATAAAAATAG AATTTTGTGTTTGGGAAATTTTTTTAAAGGGGGGAACGCGGGGCACAGGGGGGGGGAGCCCCCCCTATTA TGCCCCCAAATTTTTTTTTAGGGCGCACTTTAAAGACACACACCTGGCAGCTCGGGTGATGTGAGTGTTT CTTATTACTGTCGTGGAGCGTCCTTCGATGGAGCACACATTCATTATTATTGCGAAGATATGTGAGACAG AATGCGATGCCTGCTCATCATTGCTA >SC323400 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
CCCTGMGCATGGGCGGTAGGCKGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCAG AATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGGCGGCGCGCGCCTGGCTGG GCCCTGCGGAGCGGGAGGGAAGGAGCGAAGGAGCGAAGGAGCAAGCGGAGCGGCCGTCGCCCAAGCCAAG CCGCGCTGCCAACCCTCCCGCCCGCCCGCGCTCCTGTCCGCCGTG |
| Restriction Sites | Please inquire |
| ACCN | NM_015191 |
| Insert Size | 4730 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_015191.1, NP_056006.1 |
| RefSeq Size | 5694 bp |
| RefSeq ORF | 2781 bp |
| Locus ID | 23235 |
| UniProt ID | Q9H0K1 |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | Phosphorylates 'Ser-794' of IRS1 in insulin-stimulated adipocytes, potentially modulating the efficiency of insulin signal transduction. Inhibits CREB activity by phosphorylating and repressing TORCs, the CREB-specific coactivators.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221327 | SIK2 (Myc-DDK-tagged)-Human salt-inducible kinase 2 (SIK2) |
CNY 10,168.00 |
|
| RC221327L1 | Lenti ORF clone of Human salt-inducible kinase 2 (SIK2), Myc-DDK-tagged |
CNY 9,030.00 |
|
| RC221327L2 | Lenti ORF clone of Human salt-inducible kinase 2 (SIK2), mGFP tagged |
CNY 12,568.00 |
|
| RC221327L3 | Lenti ORF clone of Human salt-inducible kinase 2 (SIK2), Myc-DDK-tagged |
CNY 9,030.00 |
|
| RC221327L4 | Lenti ORF clone of Human salt-inducible kinase 2 (SIK2), mGFP tagged |
CNY 12,568.00 |
|
| RG221327 | SIK2 (tGFP-tagged) - Human salt-inducible kinase 2 (SIK2) |
CNY 11,768.00 |
|
| SC116431 | SIK2 (untagged)-Human salt-inducible kinase 2 (SIK2) |
CNY 6,792.00 |
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