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Snf1lk2 (SIK2) (NM_015191) Human Tagged ORF Clone
CAT＃: RC221327
SIK2 (Myc-DDK-tagged)-Human salt-inducible kinase 2 (SIK2)
ORF Plasmid: tGFP
"NM_015191" in other vectors (7)
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CNY 10,168.00
| Cited in 2 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | LOH11CR1I; QIK; SIK-2; SNF1LK2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC221327 representing NM_015191
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTCATGGCGGATGGCCCGAGGCACTTGCAGCGCGGGCCGGTCCGGGTGGGGTTCTACGACATCGAGG GCACGCTGGGCAAGGGCAACTTCGCTGTGGTGAAGCTGGGGCGGCACCGGATCACCAAGACGGAGGTGGC AATAAAAATAATCGATAAGTCTCAGCTGGATGCAGTGAACCTTGAGAAAATCTACCGAGAAGTACAAATA ATGAAAATGTTAGACCACCCTCACATAATCAAACTTTATCAGGTAATGGAGACCAAAAGTATGTTGTACC TTGTGACAGAATATGCCAAAAATGGAGAAATTTTTGACTATCTTGCTAATCATGGCCGGTTAAATGAGTC TGAAGCCAGGCGAAAATTCTGGCAAATCCTGTCTGCTGTTGATTATTGTCATGGTCGGAAGATTGTGCAC CGTGACCTCAAAGCTGAAAATCTCCTGCTGGATAACAACATGAATATCAAAATAGCAGATTTCGGTTTTG GAAATTTCTTTAAAAGTGGTGAACTGCTGGCAACATGGTGTGGCAGCCCCCCTTATGCAGCCCCAGAAGT CTTTGAAGGGCAGCAGTATGAAGGACCACAGCTGGACATCTGGAGTATGGGAGTTGTTCTTTATGTCCTT GTCTGTGGAGCTCTGCCCTTTGATGGACCGACTCTTCCAATTTTGAGGCAGAGGGTTCTGGAAGGAAGAT TCCGGATTCCGTATTTCATGTCAGAAGATTGCGAGCACCTTATCCGAAGGATGTTGGTCCTAGACCCATC CAAACGGCTAACCATAGCCCAAATCAAGGAGCATAAATGGATGCTCATAGAAGTTCCTGTCCAGAGACCT GTTCTCTATCCACAAGAGCAAGAAAATGAGCCATCCATCGGGGAGTTTAATGAGCAGGTTCTGCGACTGA TGCACAGCCTTGGAATAGATCAGCAGAAAACCATTGAGTCTTTGCAGAACAAGAGCTATAACCACTTTGC TGCCATTTATTTCTTGTTGGTGGAGCGCCTGAAATCACATCGGAGCAGTTTCCCAGTGGAGCAGAGACTT GATGGCCGCCAGCGTCGGCCTAGCACCATTGCTGAGCAAACAGTTGCCAAGGCACAGACTGTGGGGCTCC CAGTGACCATGCATTCACCGAACATGAGGCTGCTGCGATCTGCCCTCCTCCCCCAGGCATCCAACGTGGA GGCCTTTTCATTTCCAGCATCTGGCTGTCAGGCGGAAGCTGCATTCATGGAAGAAGAGTGTGTGGACACT CCAAAGGTCAATGGCTGTCTGCTTGACCCTGTGCCTCCTGTCCTGGTGCGGAAGGGATGCCAGTCACTGC CCAGCAACATGATGGAGACCTCCATTGACGAAGGGCTGGAGACAGAAGGAGAGGCCGAGGAAGACCCCGC TCATGCCTTTGAGGCATTTCAGTCCACACGCAGCGGGCAGAGACGGCACACTCTGTCAGAAGTGACCAAT CAACTGGTCGTGATGCCTGGGGCAGGGAAAATTTTCTCCATGAATGACAGCCCCTCCCTTGACAGTGTGG ACTCTGAGTATGATATGGGGTCTGTTCAGAGGGACCTGAACTTTCTGGAAGACAACCCTTCCCTTAAGGA CATCATGTTAGCCAATCAGCCTTCACCCCGCATGACATCTCCCTTCATAAGCCTGAGACCTACCAACCCA GCCATGCAGGCTCTGAGCTCCCAGAAACGAGAGGTCCACAACAGGTCTCCAGTGAGCTTCAGAGAGGGCC GCAGAGCATCAGATACCTCCCTCACCCAGGGAATTGTAGCATTTAGACAACATCTTCAGAATCTGGCTAG AACCAAAGGAATTCTAGAGTTGAACAAAGTGCAGTTGTTGTATGAACAAATAGGACCGGAGGCAGACCCT AACCTGGCGCCGGCGGCTCCTCAGCTCCAGGACCTTGCTAGCAGCTGCCCTCAGGAAGAAGTTTCTCAGC AGCAGGAAAGCGTCTCCACTCTCCCTGCCAGCGTGCATCCCCAGCTGTCCCCACGGCAGAGCCTGGAGAC CCAGTACCTGCAGCACAGACTCCAGAAGCCCAGCCTTCTGTCAAAGGCCCAGAACACCTGTCAGCTTTAT TGCAAAGAACCACCGCGGAGCCTTGAGCAGCAGCTGCAGGAACATAGGCTCCAGCAGAAGCGACTCTTTC TTCAGAAGCAGTCTCAACTGCAGGCCTATTTTAATCAGATGCAGATAGCAGAGAGCTCCTACCCACAGCC AAGTCAGCAGCTGCCCCTTCCCCGCCAGGAGACTCCACCGCCTTCTCAGCAGGCCCCACCGTTCAGCCTG ACCCAGCCCCTGAGCCCCGTCCTGGAGCCTTCCTCCGAGCAGATGCAATACAGCCCTTTCCTCAGCCAGT ACCAAGAGATGCAGCTTCAGCCCCTGCCCTCCACTTCCGGTCCCCGGGCTGCTCCTCCTCTGCCCACGCA GCTACAGCAGCAGCAGCCGCCACCGCCACCACCCCCTCCACCACCACGACAGCCAGGAGCTGCCCCAGCC CCCTTACAGTTCTCCTATCAGACTTGTGAGCTGCCAAGCGCTGCTTCCCCTGCGCCAGACTATCCCACTC CCTGTCAGTATCCTGTGGATGGAGCCCAGCAGAGCGACCTAACGGGGCCAGACTGTCCCAGAAGCCCAGG ACTGCAAGAGGCCCCCTCCAGCTACGACCCACTAGCCCTCTCTGAGCTACCTGGACTCTTTGATTGTGAA ATGCTAGACGCTGTGGATCCACAACACAACGGGTATGTCCTGGTGAAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC221327 representing NM_015191
Red=Cloning site Green=Tags(s) MVMADGPRHLQRGPVRVGFYDIEGTLGKGNFAVVKLGRHRITKTEVAIKIIDKSQLDAVNLEKIYREVQI MKMLDHPHIIKLYQVMETKSMLYLVTEYAKNGEIFDYLANHGRLNESEARRKFWQILSAVDYCHGRKIVH RDLKAENLLLDNNMNIKIADFGFGNFFKSGELLATWCGSPPYAAPEVFEGQQYEGPQLDIWSMGVVLYVL VCGALPFDGPTLPILRQRVLEGRFRIPYFMSEDCEHLIRRMLVLDPSKRLTIAQIKEHKWMLIEVPVQRP VLYPQEQENEPSIGEFNEQVLRLMHSLGIDQQKTIESLQNKSYNHFAAIYFLLVERLKSHRSSFPVEQRL DGRQRRPSTIAEQTVAKAQTVGLPVTMHSPNMRLLRSALLPQASNVEAFSFPASGCQAEAAFMEEECVDT PKVNGCLLDPVPPVLVRKGCQSLPSNMMETSIDEGLETEGEAEEDPAHAFEAFQSTRSGQRRHTLSEVTN QLVVMPGAGKIFSMNDSPSLDSVDSEYDMGSVQRDLNFLEDNPSLKDIMLANQPSPRMTSPFISLRPTNP AMQALSSQKREVHNRSPVSFREGRRASDTSLTQGIVAFRQHLQNLARTKGILELNKVQLLYEQIGPEADP NLAPAAPQLQDLASSCPQEEVSQQQESVSTLPASVHPQLSPRQSLETQYLQHRLQKPSLLSKAQNTCQLY CKEPPRSLEQQLQEHRLQQKRLFLQKQSQLQAYFNQMQIAESSYPQPSQQLPLPRQETPPPSQQAPPFSL TQPLSPVLEPSSEQMQYSPFLSQYQEMQLQPLPSTSGPRAAPPLPTQLQQQQPPPPPPPPPPRQPGAAPA PLQFSYQTCELPSAASPAPDYPTPCQYPVDGAQQSDLTGPDCPRSPGLQEAPSSYDPLALSELPGLFDCE MLDAVDPQHNGYVLVN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_015191 |
| ORF Size | 2778 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_015191.3 |
| RefSeq Size | 5694 bp |
| RefSeq ORF | 2781 bp |
| Locus ID | 23235 |
| UniProt ID | Q9H0K1 |
| Protein Families | Druggable Genome, Protein Kinase |
| MW | 103.7 kDa |
| Gene Summary | Phosphorylates 'Ser-794' of IRS1 in insulin-stimulated adipocytes, potentially modulating the efficiency of insulin signal transduction. Inhibits CREB activity by phosphorylating and repressing TORCs, the CREB-specific coactivators.[UniProtKB/Swiss-Prot Function] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
SIK2 kinase synthetic lethality is driven by spindle assembly defects in FANCA‐deficient cells
,null,
Molecular Oncology
,PubMed ID 34058059
[Snf1lk2]
|
|
Molecular Mechanisms of Tyrosine Kinase Inhibitor Resistance Induced by Membranous/Cytoplasmic/Nuclear Translocation of Epidermal Growth Factor Receptor
,Rong, X;Liang, Y;Han, Q;Zhao, Y;Jiang, G;Zhang, X;Lin, X;Liu, Y;Zhang, Y;Han, X;Zhang, M;Luo, Y;Li, P;Wei, L;Yan, T;Wang, E;,
J Thorac Oncol
,PubMed ID 31228625
[Snf1lk2]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221327L1 | Lenti ORF clone of Human salt-inducible kinase 2 (SIK2), Myc-DDK-tagged |
CNY 9,030.00 |
|
| RC221327L2 | Lenti ORF clone of Human salt-inducible kinase 2 (SIK2), mGFP tagged |
CNY 12,568.00 |
|
| RC221327L3 | Lenti ORF clone of Human salt-inducible kinase 2 (SIK2), Myc-DDK-tagged |
CNY 9,030.00 |
|
| RC221327L4 | Lenti ORF clone of Human salt-inducible kinase 2 (SIK2), mGFP tagged |
CNY 12,568.00 |
|
| RG221327 | SIK2 (tGFP-tagged) - Human salt-inducible kinase 2 (SIK2) |
CNY 11,768.00 |
|
| SC116431 | SIK2 (untagged)-Human salt-inducible kinase 2 (SIK2) |
CNY 6,792.00 |
|
| SC323400 | SIK2 (untagged)-Kinase deficient mutant (K49M) of Human salt-inducible kinase 2 (SIK2) |
CNY 6,792.00 |
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