United States
Germany
Japan
United Kingdom
China
STK16 (NM_003691) Human Untagged Clone
CAT＃: SC323397
STK16 (untagged)-Kinase deficient mutant (K49M) of Human serine/threonine kinase 16 (STK16), transcript variant 1
CNY 2,400.00
CNY 6,270.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | FLJ39635; KRCT; KRCT, MPSK, TSF1, PKL12, FLJ39635; MPSK; PKL12; protein kinase expressed in day 12 fetal liver; serine/threonine kinase 16; TSF1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323397 sequence for NM_003691 edited (data generated by NextGen Sequencing)
ATGGGCCACGCGCTGTGTGTCTGCTCTCGGGGAACTGTCATCATTGACAATAAGCGCTAC CTCTTCATCCAGAAACTGGGGGAGGGTGGGTTCAGCTATGTGGACCTAGTGGAAGGGTTA CATGATGGACACTTCTACGCCCTGATGCGAATCCTGTGTCACGAGCAGCAGGACCGGGAG GAGGCCCAGCGAGAAGCCGACATGCATCGCCTCTTCAATCACCCCAACATCCTTCGCCTC GTGGCTTACTGTCTGAGGGAACGGGGTGCTAAGCATGAGGCCTGGCTGCTGCTACCATTC TTCAAGAGAGGTACGCTGTGGAATGAGATAGAAAGGCTGAAGGACAAAGGCAACTTCCTG ACCGAGGATCAAATCCTTTGGCTGCTGCTGGGGATCTGCAGAGGCCTTGAGGCCATTCAT GCCAAGGGTTATGCCCACAGAGACTTGAAGCCCACCAATATATTGCTTGGAGATGAGGGG CAGCCAGTTTTAATGGACTTGGGTTCCATGAATCAAGCATGCATCCATGTGGAGGGCTCC CGCCAGGCTCTGACCCTGCAGGACTGGGCAGCCCAGCGGTGCACCATCTCCTACCGAGCC CCAGAGCTCTTCTCTGTGCAGAGTCACTGTGTCATCGATGAGCGGACTGATGTCTGGTCC CTAGGCTGCGTGCTATATGCCATGATGTTTGGGGAAGGCCCTTATGACATGGTGTTCCAA AAGGGTGACAGTGTGGCCCTTGCTGTGCAGAACCAACTCAGCATCCCACAAAGCCCCAGG CATTCTTCAGCATTGTGGCAGCTCCTGAACTCGATGATGACCGTGGACCCGCATCAGCGT CCTCACATTCCTCTCCTCCTCAGTCAGCTGGAGGCGCTGCAGCCCCCAGCTCCTGGCCAA CATACTACCCAAATCTGA Clone variation with respect to NM_003691.2 146 a=>t;796 c=>t >OriGene 5' read for mutant NM_003691 unedited
ACCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGCGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTTCTCTCTGGCCC GAGCCAGCATGATCCGCTGGGCCCCCAGCGCATCTCCTGGAAGAGCCCACTCACCCTGGACGAGCTCTTC GGTAGCCTCAGACCGTCCTTGAAGAGGATGACTGAGACATTATGGGCCACGCGCTGTGTGTCTGCTCTCG GGGAACTGTCATCATTGACAATAAGCGCTACCTCTTCATCCAGAAACTGGGGGGAGGGTGGGGTTTCTGC TATTGTGGACCTAGTGGAAGGGTTACATGATGGGACACTTTCTACCGCCCCTGATGCGAATCTGGTGTCC ACGAAGCAGCAGGACCCGGGAGGAAGGCCCAGCGAAGAAGCCGACATGGCATCCGCCCTCTTCATTCACC CCAACCATCCTTCGCCCCTGGTGGCCTTACTGTCTAGAGGGAACGGGGTGCTAAGCATGAGGCCTGCCTG CTGCTCCCATTCTTAAAAAAAGGTACCCGGGGGAATGAGATAAAAAGGGCTAAAGAGACAAAGGCCATTT CCTGACGAGGGATCAAACTCTTTGGTCTGGCGTGTGGGGATTCGCAGAGCCCTGTGAGGCCATCTCGCCC AGGGTATATGCCACAGACATGTAGACCCACACTATATTTTGCTGGATATGAGGCGCCACCTTTATAGGGC ACGTGGTCTCGAGATACACTCTGCATCGTGTAGAGGCTCCCACGTCTACACCTGATGTGAACACAGTGTG CACTATCCTAGACCAGAGCTTTTGTGACACTCGTGTCCATAGACGAGTTGTGCCACATGCGTCTCTACCA GTTGGAAGC >SC323397 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
CSACGMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTC AGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTTCTCTCTGGCCCGAGCC AGCATGATCCGCTGGGCCCCCAGCGCATCTCCTGGAAGAGCCCACTCACCCTGGACGAGCTCTTCGGTAG CCTCAGACCGTCCTTGAAGAGGATGACTGAGACATTATGGGCCAC |
| Restriction Sites | Please inquire |
| ACCN | NM_003691 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_003691.2, NP_003682.2 |
| RefSeq Size | 1682 bp |
| RefSeq ORF | 918 bp |
| Locus ID | 8576 |
| Domains | pkinase, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | Membrane-associated protein kinase that phosphorylates on serine and threonine residues. In vitro substrates include DRG1, ENO1 and EIF4EBP1. Also autophosphorylates. May be involved in secretory vesicle trafficking or intracellular signaling. May have a role in regulating stromal-epithelial interactions that occur during ductal morphogenesis in the mammary gland. May be involved in TGF-beta signaling. Able to autophosphorylate on Tyr residue; it is however unclear whether it has tyrosine-protein kinase toward other proteins.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200566 | STK16 (Myc-DDK-tagged)-Human serine/threonine kinase 16 (STK16), transcript variant 1 |
CNY 2,400.00 |
|
| RC200566L1 | Lenti ORF clone of Human serine/threonine kinase 16 (STK16), transcript variant 1, Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC200566L2 | Lenti ORF clone of Human serine/threonine kinase 16 (STK16), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC200566L3 | Lenti ORF clone of Human serine/threonine kinase 16 (STK16), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC200566L4 | Lenti ORF clone of Human serine/threonine kinase 16 (STK16), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG200566 | STK16 (tGFP-tagged) - Human serine/threonine kinase 16 (STK16), transcript variant 1 |
CNY 4,000.00 |
|
| SC319372 | STK16 (untagged)-Human serine/threonine kinase 16 (STK16), transcript variant 1 |
CNY 2,400.00 |
