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CKII alpha (CSNK2A1) (NM_001895) Human Untagged Clone
CAT＃: SC323395
CSNK2A1 (untagged)-Kinase deficient mutant (K68M) of Human casein kinase 2, alpha 1 polypeptide (CSNK2A1), transcript variant 2
CNY 3,656.00
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CK2A1; Cka1; Cka2; CKII; OCNDS |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323395 sequence for NM_001895 edited (data generated by NextGen Sequencing)
ATGTCGGGACCCGTGCCAAGCAGGGCCAGAGTTTACACAGATGTTAATACACACAGACCT CGAGAATACTGGGATTACGAGTCACATGTGGTGGAATGGGGAAATCAAGATGACTACCAG CTGGTTCGAAAATTAGGCCGAGGTAAATACAGTGAAGTATTTGAAGCCATCAACATCACA AATAATGAAAAAGTTGTTGTTATGATTCTCAAGCCAGTAAAAAAGAAGAAAATTAAGCGT GAAATAAAGATTTTGGAGAATTTGAGAGGAGGTCCCAACATCATCACACTGGCAGACATT GTAAAAGACCCTGTGTCACGAACCCCCGCCTTGGTTTTTGAACACGTAAACAACACAGAC TTCAAGCAATTGTACCAGACGTTAACAGACTATGATATTCGATTTTACATGTATGAGATT CTGAAGGCCCTGGATTATTGTCACAGCATGGGAATTATGCACAGAGATGTCAAGCCCCAT AATGTCATGATTGATCATGAGCACAGAAAGCTACGACTAATAGACTGGGGTTTGGCTGAG TTTTATCATCCTGGCCAAGAATATAATGTCCGAGTTGCTTCCCGATACTTCAAAGGTCCT GAGCTACTTGTAGACTATCAGATGTACGATTATAGTTTGGATATGTGGAGTTTGGGTTGT ATGCTGGCAAGTATGATCTTTCGGAAGGAGCCATTTTTCCATGGACATGACAATTATGAT CAGTTGGTGAGGATAGCCAAGGTTCTGGGGACAGAAGATTTATATGACTATATTGACAAA TACAACATTGAATTAGATCCACGTTTCAATGATATCTTGGGCAGACACTCTCGAAAGCGA TGGGAACGCTTTGTCCACAGTGAAAATCAGCACCTTGTCAGCCCTGAGGCCTTGGATTTC CTGGACAAACTGCTGCGATATGACCACCAGTCACGGCTTACTGCAAGAGAGGCAATGGAG CACCCCTATTTCTACACTGTTGTGAAGGACCAGGCTCGAATGGGTTCATCTAGCATGCCA GGGGGCAGTACGCCCGTCAGCAGCGCCAATATGATGTCAGGGATTTCTTTAGTGCCAACC CCTTCACCCCTTGGACCTCTGGCAGGCTCACCAGTGATTGCTGCTGCCAACCCCCTTGGG ATGCCTGTTCCAGCTGCCGCTGGCGCTCAGCAGTAA Clone variation with respect to NM_001895.3 203 a=>t;204 a=>g;1070 c=>t >OriGene 5' read for mutant NM_001895 unedited
CCGCCCGTCCAGCAACGGGCGGTAGGCGCGTACGGCGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA CCGTCAGAATCTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGAGAGCGGCCGCC GCCGCTGCCGCTTCCACCACAGTTTGAAGAAAACAGGTCTGAAACAAGGTCTTACCCCCAGCTGCTTCTG AACACAGTGACTGCCAGATCTCCAAACATCAAGTCCAGCTTTGTCCGCCAACCTGTCTGACATGTCGGGA CCCGTGCCAAGCAGGGCCAGAGTTTACACAGATGTTAATACACACAGACCTCGAGAATACTGGTGATTAC TGAGTCACATGTGGTGGAATGGGGAAATCAAGATGACTACCCAGCTGGTTCGAAAATTAGGCCGAGGTGA ATTACAGTTGAAGTATTTGAAGCCCATCACATCACAAATAATGAAAAAGTTGTTGGTTATGATTCTCAGC CAGTAAAAAAGAAGAAAATTAGCGTGAAATAAGATTTGGAGATTTGGAAGGAGGTCCCAACTCATCACCT GCAGGACATTGAAAAGACCTGGGTAAGAACCCCCGCCTGGTTTTTGAACCGTAACCACCGATTCAGCATT GTCCAACTTACGACATGGATTCGATTTACGGTAAATCGAGCCTGATATGCCAACTGGATTAGCAGAATTC AGCCTATGTCGATGACTGGCCGGAGACCACTAGACTGGTGCGATTAAACTGCCAATATGCAAGTTCGGAC TCAGTCGACATGACTGATGCAATTGATTGATGGGCGGCATTTT >SC323395 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
CKCTCGAATAGGCCGAGGTAATACAGTGAAGTATTTGAAGCCATCAACATCACAAATAATGAAAAAGTTG TTGTTATGATTCTCAAGCCAGTAAAAAAGAAGAAAATTAAGCGTGAAATAAAGATTTTGGAGAATTTGAG AGGAGGTCCCAACATCATCACACTGGCAGACATTGTAAAAGACCCTGTGTCACGAACCCCCGCCTTGGTT TTTGAACACGTAAACAACACAGACTTCAAGCAATTGTACCAGACG |
| Restriction Sites | Please inquire |
| ACCN | NM_001895 |
| Insert Size | 2680 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001895.3, NP_001886.1 |
| RefSeq Size | 2732 bp |
| RefSeq ORF | 1176 bp |
| Locus ID | 1457 |
| UniProt ID | P68400 |
| Domains | pkinase, TyrKc, S_TKc |
| Protein Families | Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase |
| Protein Pathways | Adherens junction, Tight junction, Wnt signaling pathway |
| Gene Summary | Casein kinase II is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. It is involved in various cellular processes, including cell cycle control, apoptosis, and circadian rhythm. The kinase exists as a tetramer and is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. The protein encoded by this gene represents the alpha subunit. Multiple transcript variants encoding different protein isoforms have been found for this gene. [provided by RefSeq, Apr 2018] Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2, 4 and 5 encode the same protein. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222302 | CSNK2A1 (Myc-DDK-tagged)-Human casein kinase 2, alpha 1 polypeptide (CSNK2A1), transcript variant 2 |
CNY 3,656.00 |
|
| RC222302L1 | Lenti-ORF clone of CSNK2A1 (Myc-DDK-tagged)-Human casein kinase 2, alpha 1 polypeptide (CSNK2A1), transcript variant 2 |
CNY 6,056.00 |
|
| RC222302L2 | Lenti-ORF clone of CSNK2A1 (mGFP-tagged)-Human casein kinase 2, alpha 1 polypeptide (CSNK2A1), transcript variant 2 |
CNY 5,890.00 |
|
| RC222302L3 | Lenti-ORF clone of CSNK2A1 (Myc-DDK-tagged)-Human casein kinase 2, alpha 1 polypeptide (CSNK2A1), transcript variant 2 |
CNY 5,890.00 |
|
| RC222302L4 | Lenti-ORF clone of CSNK2A1 (mGFP-tagged)-Human casein kinase 2, alpha 1 polypeptide (CSNK2A1), transcript variant 2 |
CNY 6,056.00 |
|
| RG222302 | CSNK2A1 (tGFP-tagged) - Human casein kinase 2, alpha 1 polypeptide (CSNK2A1), transcript variant 2 |
CNY 5,256.00 |
|
| SC109127 | CSNK2A1 (untagged)-Human casein kinase 2, alpha 1 polypeptide (CSNK2A1), transcript variant 2 |
CNY 3,656.00 |
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