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PRKACG (NM_002732) Human Untagged Clone
CAT＃: SC323393
PRKACG (untagged)-Kinase deficient mutant (K73M) of Human protein kinase, cAMP-dependent, catalytic, gamma (PRKACG)
CNY 3,656.00
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | BDPLT19; KAPG; PKACg |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323393 sequence for NM_002732 edited (data generated by NextGen Sequencing)
ATGGGCAACGCCCCCGCCAAGAAGGACACCGAGCAGGAGGAGAGCGTGAACGAGTTCCTA GCCAAAGCCAGAGGAGATTTCCTCTACAGATGGGGAAACCCCGCTCAAAACACCGCCAGC TCGGATCAGTTCGAACGGCTCAGGACGCTGGGCATGGGCTCCTTCGGGCGGGTGATGCTG GTGAGGCACCAGGAGACCGGCGGCCACTACGCCATGATGATCCTCAACAAGCAGAAGGTG GTGAAGATGAAGCAGGTCGAGCACATACTGAACGAGAAGCGCATCCTGCAGGCGATCGAC TTTCCGTTCCTCGTCAAACTCCAGTTCTCCTTTAAGGACAACTCCTACCTGTACCTGGTG ATGGAGTACGTGCCGGGTGGGGAGATGTTCTCCCGCCTACAGCGCGTCGGAAGGTTTAGC GAGCCCCATGCCTGTTTCTATGCCGCCCAGGTCGTCCTGGCCGTCCAGTACCTACACTCG CTCGACCTCATCCACCGCGACCTGAAGCCCGAGAATCTCCTCATCGACCAGCAGGGCTAC CTGCAGGTGACGGACTTCGGTTTCGCCAAGCGCGTGAAGGGCCGCACTTGGACCTTGTGC GGGACCCCAGAGTACCTGGCCCCCGAGATCATCCTGAGCAAAGGCTACAACAAGGCCGTG GACTGGTGGGCCCTAGGGGTGCTCATCTATGAGATGGCCGTGGGCTTCCCACCCTTCTAC GCCGACCAGCCCATCCAGATCTACGAGAAGATCGTCTCTGGGAGGGTGCGGTTTCCCTCC AAACTCAGCTCTGACCTCAAGCATCTGCTGCGGAGCCTGCTGCAGGTGGACCTCACCAAG CGCTTCGGAAACCTCAGGAACGGGGTTGGCGACATCAAGAACCACAAGTGGTTCGCCACA ACCAGCTGGATCGCCATCTATGAGAAGAAGGTGGAAGCTCCCTTCATCCCGAAGTACACA GGCCCTGGGGATGCCAGTAACTTTGACGACTACGAGGAGGAAGAGCTCCGGATCTCCATC AATGAGAAGTGTGCCAAGGAGTTTTCTGAGTTTTAG Clone variation with respect to NM_002732.3 218 a=>t;318 g=>a >OriGene 5' read for mutant NM_002732 unedited
CCCGCCCGTTGAGCAATGGGCGGTAGGCGTGTACGGATGCGTGAGGTCTATATAAGCAGAGCTCGTTTAG TGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGCCCCTGCCGCC GCCACCGCCATGGGCAACGCCCCCGCCAAGAAGGACACCGAGCAGGAGGAGAGCGTGAACGAGTTCCTAG CCAAAGCCAGAGGAGATTTCCTCTACAGATGGGGAAACCCCGCTCAAAACACCGCCAGCTCGGATCAGTT CGAACGGCTCAGGACGCTGGGCATGGGCTCCTTCGGGCGGGTGATGCTGGTGAGGCACCAGGAGACCGGC GGCCACTACGCCATGATGATCCTCAACAAGCAGACGTGGTGAAGATGAAGCAGTCGAGCACATACTGACG AGAGCGCATCCTGCAGGCGATCGACTTTCCGTTCCTCGTCAACTCCAGTTCTCCTTTAGGACACTCCTAC CTGTACCTGTGATGAGTACGTGCTGGTGGGAGATGTCTCCGCTACAGCGCGTCGAGGTTACGAGCCATGC TGTTCATGCCGCCAGTCGTCTGCGTCAGTACTACATCGCTCGACCTATCACCGGGACTGGAGCCGAAATC TCTATCGACCACAGGCCTACCTGGAGGTGAACGGACTCGGTTTCCAGCCGTGAAGGCCGACTTGGACCTT GGCGGAACAGATACCTGCCTGATATCTCTGACTAGGTCAACACGGGCTGACGTGACCATAGGGCTCTATT AGAATGCCTGCTTCACCTACGCAACGCACATCTGAGATGTTCTGAGGACTGCGTTCTCACCTCTGATCAC TGAGCGCAATCTGAAGAGA >SC323393 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
CSACGMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTC AGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGCCCCTGCCGCCGCCACCGCC ATGGGCAACGCCCCCGCCAAGAAGGACACCGAGCAGGAGGAGAGCGTGAACGAGTTCCTAGCCAAAGCCA GAGGAGATTTCCTCTACAGATGGGGAAACCCCGCTCAAAACACCG |
| Restriction Sites | Please inquire |
| ACCN | NM_002732 |
| Insert Size | 2100 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002732.2, NP_002723.2 |
| RefSeq Size | 1560 bp |
| RefSeq ORF | 1056 bp |
| Locus ID | 5568 |
| UniProt ID | P22612 |
| Domains | pkinase, S_TK_X, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Apoptosis, Calcium signaling pathway, Chemokine signaling pathway, Dilated cardiomyopathy, Gap junction, GnRH signaling pathway, Hedgehog signaling pathway, Insulin signaling pathway, Long-term potentiation, MAPK signaling pathway, Melanogenesis, Olfactory transduction, Oocyte meiosis, Prion diseases, Progesterone-mediated oocyte maturation, Taste transduction, Vascular smooth muscle contraction, Vibrio cholerae infection, Wnt signaling pathway |
| Gene Summary | Cyclic AMP-dependent protein kinase (PKA) consists of two catalytic subunits and a regulatory subunit dimer. This gene encodes the gamma form of its catalytic subunit. The gene is intronless and is thought to be a retrotransposon derived from the gene for the alpha form of the PKA catalytic subunit. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207946 | PRKACG (Myc-DDK-tagged)-Human protein kinase, cAMP-dependent, catalytic, gamma (PRKACG) |
CNY 3,656.00 |
|
| RC207946L1 | Lenti ORF clone of Human protein kinase, cAMP-dependent, catalytic, gamma (PRKACG), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC207946L2 | Lenti ORF clone of Human protein kinase, cAMP-dependent, catalytic, gamma (PRKACG), mGFP tagged |
CNY 5,890.00 |
|
| RC207946L3 | Lenti ORF clone of Human protein kinase, cAMP-dependent, catalytic, gamma (PRKACG), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC207946L4 | Lenti ORF clone of Human protein kinase, cAMP-dependent, catalytic, gamma (PRKACG), mGFP tagged |
CNY 5,890.00 |
|
| RG207946 | PRKACG (tGFP-tagged) - Human protein kinase, cAMP-dependent, catalytic, gamma (PRKACG) |
CNY 5,256.00 |
|
| SC118449 | PRKACG (untagged)-Human protein kinase, cAMP-dependent, catalytic, gamma (PRKACG) |
CNY 3,656.00 |
