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RPS6KA3 (NM_004586) Human Untagged Clone
CAT＃: SC323378
RPS6KA3 (untagged)-Kinase deficient mutant (K100M) of Human ribosomal protein S6 kinase, 90kDa, polypeptide 3 (RPS6KA3)
CNY 5,432.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CLS; HU-3; ISPK-1; MAPKAPK1B; MRX19; p90-RSK2; pp90RSK2; RSK; RSK2; S6K-alpha3 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323378 sequence for NM_004586 edited (data generated by NextGen Sequencing)
ATGCCGCTGGCGCAGCTGGCGGACCCGTGGCAGAAGATGGCTGTGGAGAGCCCGTCCGAC AGCGCTGAGAATGGACAGCAAATTATGGATGAACCTATGGGAGAGGAGGAGATTAACCCA CAAACTGAAGAAGTCAGTATCAAAGAAATTGCAATCACACATCATGTAAAGGAAGGACAT GAAAAGGCAGATCCTTCCCAGTTTGAACTTTTAAAAGTATTAGGGCAGGGATCATTTGGA AAGGTTTTCTTAGTTAAAAAAATCTCAGGCTCTGATGCTAGGCAGCTTTATGCCATGATG GTATTGAAGAAGGCCACACTGAAAGTTCGAGACCGAGTTCGGACAAAAATGGAACGTGAT ATCTTGGTAGAGGTTAATCATCCTTTTATTGTCAAGTTGCATTATGCTTTTCAAACTGAA GGGAAGTTGTATCTTATTTTGGATTTTCTCAGGGGAGGAGATTTGTTTACACGCTTATCC AAAGAGGTGATGTTCACAGAAGAAGATGTCAAATTCTACTTGGCTGAACTTGCACTTGCT TTAGACCATCTACATAGCCTGGGAATAATTTATAGAGACTTAAAACCAGAAAATATACTT CTTGATGAAGAAGGTCACATCAAGTTAACAGATTTCGGCCTAAGTAAAGAGTCTATTGAC CATGAAAAGAAGGCATATTCTTTTTGTGGAACTGTGGAGTATATGGCTCCAGAAGTAGTT AATCGTCGAGGTCATACTCAGAGTGCTGACTGGTGGTCTTTTGGTGTGTTAATGTTTGAA ATGCTTACTGGTACACTACCTTTCCAAGGAAAAGATCGAAAAGAAACAATGACTATGATT CTTAAAGCCAAACTTGGAATGCCACAGTTTTTGAGTCCTGAAGCGCAGAGTCTTTTACGA ATGCTTTTCAAGCGAAATCCTGCAAACAGATTAGGTGCAGGACCAGATGGAGTTGAAGAA ATTAAAAGACATTCATTTTTCTCAACGATAGACTGGAATAAACTGTATAGAAGAGAAATT CATCCGCCATTTAAACCTGCAACGGGCAGGCCTGAAGATACATTCTATTTTGATCCTGAG TTTACTGCAAAAACTCCCAAAGATTCACCTGGCATTCCACCTAGTGCTAATGCACATCAG CTTTTTCGGGGGTTTAGTTTTGTTGCTATTACCTCAGATGATGAAAGCCAAGCTATGCAG ACAGTTGGTGTACATTCAATTGTTCAGCAGTTACACAGGAACAGTATTCAGTTTACTGAT GGATATGAAGTAAAAGAAGATATTGGAGTTGGCTCCTACTCTGTTTGCAAGAGATGTATA CATAAAGCTACAAACATGGAGTTTGCAGTGAAGATTATTGATAAAAGCAAGAGAGACCCA ACAGAAGAAATTGAAATTCTTCTTCGTTATGGACAGCATCCAAACATTATCACTCTAAAG GATGTATATGATGATGGAAAGTATGTGTATGTAGTAACAGAACTTATGAAAGGAGGTGAA TTGCTGGATAAAATTCTTAGACAAAAATTTTTCTCTGAACGAGAGGCCAGTGCTGTCCTG TTCACTATAACTAAAACCGTTGAATATCTTCACGCACAAGGGGTGGTTCATAGAGACTTG AAACCTAGCAACATTCTTTATGTGGATGAATCTGGTAATCCGGAATCTATTCGAATTTGT GATTTTGGCTTTGCAAAACAGCTGAGAGCGGAAAATGGTCTTCTCATGACTCCTTGTTAC ACTGCAAATTTTGTTGCACCAGAGGTTTTAAAAAGACAAGGCTATGATGCTGCTTGTGAT ATATGGAGTCTTGGTGTCCTACTCTATACAATGCTTACCGGTTACACTCCATTTGCAAAT GGTCCTGATGATACACCAGAGGAAATATTGGCACGAATAGGTAGCGGAAAATTCTCACTC AGTGGTGGTTACTGGAATTCTGTTTCAGACACAGCAAAGGACCTGGTGTCAAAGATGCTT CATGTAGACCCTCATCAGAGACTGACTGCTGCTCTTGTGCTCAGACATCCTTGGATCGTC CACTGGGACCAACTGCCACAATACCAACTAAACAGACAGGATGCACCACATCTAGTAAAG GGTGCCATGGCAGCTACATATTCTGCTTTGAACCGTAATCAGTCACCAGTTTTGGAACCA GTAGGCCGCTCTACTCTTGCTCAGCGGAGAGGTATTAAAAAAATCACCTCAACAGCCCTG TGA Clone variation with respect to NM_004586.2 299 a=>t;798 c=>a >OriGene 5' read for mutant NM_004586 unedited
CCCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGAGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGAGGAGGCGGTGAAG GCGGCGGGCCGGGGGGGAAGATGCCGCTGGCGCAGCTGGCGGACCCGTGGCAGAAGATGGCTGTGGAGAG CCCGTCCGACAGCGCTGAGGATTATATCTTTATCCTTGAAATTTAAAAACATTTGCCAGGACATGTCTTG GTGTTGATGATCTCTGTTGAACCTTCTCAGTCTCCAGTCTCAGGATAAATGAGGAGCTGATGGTTAATCC CGGTCACATTGAATGGACAGCAAATTATGGGATGAACCCTATGGGAGAGGAGGAGATTAGCCCACAACTG AAGAAGTCAGGTGTCAAGGAAATTGCATCCCACATCTGGTAAGGAGGGCATGAAAAGGCGGATCCTTCCA GTTTGAACTTTAAAATATTAGGGCAGGATCATTTGGAAGGGTTTTCTAGTAAAAAATCTCAGGCTCTGAG CTAGGCACCTTTTGCCTGGAGGGTATTGAAAGGCCACCGAAGGTTCAGACCGATTTCGACAATGGACTGG ATCTGTAAGGTAACTCCTTATATGACATTCTTGCTTTCACGAGGGATTGACTTTTTTGTTTTTCAGAGAG AGGGTACCGTACCAGAGAGAGTACGAGGGATACAACATAGG >SC323378 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
CSCTTGMGCATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTC AGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGAGGAGGCGGTGAAGGCGGC GGGCCGGGGGGGAAGATGCCGCTGGCGCAGCTGGCGGACCCGTGGCAGAAGATGGCTGTGGAGAGCCCGT CCGACAGCGCTGAGGATTATATCTTTATCCTTGAAATTTAAAAAC |
| Restriction Sites | Please inquire |
| ACCN | NM_004586 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_004586.2, NP_004577.1 |
| RefSeq Size | 7723 bp |
| RefSeq ORF | 2223 bp |
| Locus ID | 6197 |
| UniProt ID | P51812 |
| Domains | pkinase, S_TK_X, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Long-term potentiation, MAPK signaling pathway, mTOR signaling pathway, Neurotrophin signaling pathway, Oocyte meiosis, Progesterone-mediated oocyte maturation |
| Gene Summary | This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Mutations in this gene have been associated with Coffin-Lowry syndrome (CLS). [provided by RefSeq, Jul 2008] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC213645 | RPS6KA3 (Myc-DDK-tagged)-Human ribosomal protein S6 kinase, 90kDa, polypeptide 3 (RPS6KA3) |
CNY 5,424.00 |
|
| RC213645L1 | Lenti ORF clone of Human ribosomal protein S6 kinase, 90kDa, polypeptide 3 (RPS6KA3), Myc-DDK-tagged |
CNY 7,824.00 |
|
| RC213645L2 | Lenti ORF clone of Human ribosomal protein S6 kinase, 90kDa, polypeptide 3 (RPS6KA3), mGFP tagged |
CNY 9,600.00 |
|
| RC213645L3 | Lenti ORF clone of Human ribosomal protein S6 kinase, 90kDa, polypeptide 3 (RPS6KA3), Myc-DDK-tagged |
CNY 9,600.00 |
|
| RC213645L4 | Lenti ORF clone of Human ribosomal protein S6 kinase, 90kDa, polypeptide 3 (RPS6KA3), mGFP tagged |
CNY 7,824.00 |
|
| RG213645 | RPS6KA3 (tGFP-tagged) - Human ribosomal protein S6 kinase, 90kDa, polypeptide 3 (RPS6KA3) |
CNY 7,024.00 |
|
| SC314588 | RPS6KA3 (untagged)-Human ribosomal protein S6 kinase, 90kDa, polypeptide 3 (RPS6KA3) |
CNY 14,160.00 |
|
| SC323412 | RPS6KA3 (untagged)-Kinase deficient mutant (K100M) of Human ribosomal protein S6 kinase, 90kDa, polypeptide 3 (RPS6KA3) |
CNY 5,432.00 |
|
| SC323677 | RPS6KA3 (untagged)-Kinase deficient mutant (K451M) of Human ribosomal protein S6 kinase, 90kDa, polypeptide 3 (RPS6KA3) |
CNY 5,432.00 |
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