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HIPK4 (NM_144685) Human Untagged Clone
CAT＃: SC323367
HIPK4 (untagged)-Kinase deficient mutant (K40M) of Human homeodomain interacting protein kinase 4 (HIPK4)
CNY 4,592.00
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323367 sequence for NM_144685 edited (data generated by NextGen Sequencing)
ATGTCCACCATCCAGTCGGAGACTGACTGCTACGACATCATCGAGGTCTTGGGCAAGGGG ACCTTCGGGGAGGTAGCCAAGGGCTGGCGGCGGAGCACGGGCGAGATGGTGGCCATCATG ATCCTCAAGAATGACGCCTACCGCAACCGCATCATCAAAAACGAGCTGAAGCTGCTGCAC TGCATGCGAGGCCTAGACCCTGAAGAGGCCCACGTCATCCGCTTCCTTGAGTTCTTCCAT GACGCCCTCAAGTTCTACCTGGTCTTTGAGCTGCTGGAGCAAAACCTTTTCGAGTTCCAG AAGGAGAACAACTTCGCGCCCCTCCCCGCCCGCCACATCCGTACAGTCACCCTGCAGGTG CTCACAGCCCTGGCCCGGCTCAAGGAGCTGGCTATCATCCACGCTGATCTCAAGCCTGAG AACATCATGCTGGTGGACCAGACCCGCTGCCCCTTCAGGGTCAAGGTGATTGACTTCGGA TCCGCCAGCATTTTCAGCGAGGTGCGCTACGTGAAGGAGCCATACATCCAGTCGCGCTTC TACCGGGCCCCTGAGATCCTGCTGGGGCTGCCCTTCTGCGAGAAGGTGGACGTGTGGTCC CTGGGCTGCGTCATGGCTGAGCTGCACCTGGGCTGGCCTCTCTACCCCGGCAACAACGAG TACGACCAGGTGCGCTACATCTGCGAAACCCAGGGCCTGCCCAAGCCACACCTGTTGCAC GCCGCCTGCAAGGCCCACCACTTCTTCAAGCGCAACCCCCACCCTGACGCTGCCAACCCC TGGCAGCTCAAGTCCTCGGCTGACTACCTGGCCGAGACGAAGGTGCGCCCATTGGAGCGC CGCAAGTATATGCTCAAGTCGTTGGACCAGATTGAGACAGTGAATGGTGGCAGTGTGGCC AGTCGGCTAACCTTCCCTGACCGGGAGGCGCTGGCGGAGCACGCCGACCTCAAGAGCATG GTGGAGCTGATCAAGCGCATGCTGACCTGGGAGTCACACGAACGCATCAGCCCCAGTGCT GCCCTGCGCCACCCCTTCGTGTCCATGCAGCAGCTGCGCAGTGCCCACGAGACCACCCAC TACTACCAGCTCTCGCTGCGCAGCTACCGCCTCTCGCTGCAAGTGGAGGGGAAGCCCCCC ACGCCCGTCGTGGCCGCAGAAGATGGGACCCCCTACTACTGTCTGGCTGAGGAGAAGGAG GCTGCGGGTATGGGCAGTGTGGCCGGCAGCAGCCCCTTCTTCCGAGAGGAGAAGGCACCA GGTATGCAAAGAGCCATCGACCAGCTGGATGACCTGAGTCTGCAGGAGGCTGGGCATGGG CTGTGGGGTGAGACCTGCACCAATGCGGTCTCCGACATGATGGTCCCCCTCAAGGCAGCC ATCACTGGCCACCATGTGCCCGACTCGGGCCCTGAGCCCATCCTGGCCTTCTACAGCAGC CGCCTGGCAGGCCGCCACAAGGCCCGCAAGCCACCTGCGGGTTCCAAGTCCGACTCCAAC TTCAGCAACCTCATTCGGCTGAGCCAGGTCTCGCCTGAGGATGACAGGCCCTGCCGGGGC AGCAGCTGGGAGGAAGGAGAGCATCTCGGGGCCTCTGCTGAGCCACTGGCCATCCTGCAG CGAGATGAGGATGGGCCCAACATTGACAACATGACCATGGAAGCTGAGAGGCCAGACCCT GAGCTCTTCGACCCCAGCAGCTGTCCTGGAGAATGGCTGAGTGAGCCAGACTGCACCCTG GAGAGCGTCAGGGGCCCACGGGCTCAGGGGCTCCCACCCCGCCGCTCCCACCAGCATGGT CCACCCCGGGGGGCCACCAGCTTCCTCCAGCATGTCACCGGGCACCACTGA Clone variation with respect to NM_144685.3 119 a=>t >OriGene 5' read for mutant NM_144685 unedited
CCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAAC CGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCATAACTTCGTATAGCATACATTATACGAAG TTATGGATCAGGCCAAATCGGCCGAGCTCGAATTCGTCGAGAGCGGAGAGCCACTCAACAGCGCTGGAAC CCATTCGGTGGGGCCTGGGGCCCCTCATCCCAAGCCAGGAGGGTTTCTGGGGAGGGGTGCAGCCCCCTGG GCAGACTGACAGTGTGGGCCTGGGGGGTTTTGGGGGTGCCAGGGAAACAGGGCCCAACCCCCTGGGGGGG GTGGGTTAAGTGCGGTTTTCCTTTTCCCCACTGGGGGCCCTCCGTATTTCCCCATAGGGGGGGGGGGGGG GGGGGGCAGAACCGGGGGACCCAGAAAAAAGGGAAAGCCGGTGTTCGAAAAGTGAGGGGGGAATTCAAGG AACAGGGGGAGGAAAGACCCCCCAAGAAACAAGCAGAAAGGGCGGGGGACAT >SC323367 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
CSTTGMGCAATGGGCGGTAGGCKGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCA GAATTTTGTAATACGACTCACTATAGGGCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATG GATCAGGCCAAATCGGCCGAGCTCGAATTCGTCGAGAGCGGAGAGCCACTCAACAGCGCTGGAACCCATT CGGTGGGGCCTGGGGCCCCTCATCCCAAGCCAGGAGGGTTTCTGG |
| Restriction Sites | Please inquire |
| ACCN | NM_144685 |
| Insert Size | 2472 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_144685.3, NP_653286.2 |
| RefSeq Size | 2467 bp |
| RefSeq ORF | 1851 bp |
| Locus ID | 147746 |
| UniProt ID | Q8NE63 |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | This gene encodes a member of the homeodomain interacting protein kinase (HIPK) family of proteins. While other members of this family are found throughout vertebrates, this member is present only in mammals. Compared to other members of this family, the encoded protein lacks a nuclear localization signal and a C-terminal autoinhibitory domain. The encoded protein exhibits kinase activity and may phosphorylate the tumor suppressor protein p53. [provided by RefSeq, Jul 2016] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205549 | HIPK4 (Myc-DDK-tagged)-Human homeodomain interacting protein kinase 4 (HIPK4) |
CNY 4,584.00 |
|
| RC205549L1 | Lenti-ORF clone of HIPK4 (Myc-DDK-tagged)-Human homeodomain interacting protein kinase 4 (HIPK4) |
CNY 6,984.00 |
|
| RC205549L2 | Lenti-ORF clone of HIPK4 (mGFP-tagged)-Human homeodomain interacting protein kinase 4 (HIPK4) |
CNY 5,890.00 |
|
| RC205549L3 | Lenti-ORF clone of HIPK4 (Myc-DDK-tagged)-Human homeodomain interacting protein kinase 4 (HIPK4) |
CNY 5,890.00 |
|
| RC205549L4 | Lenti-ORF clone of HIPK4 (mGFP-tagged)-Human homeodomain interacting protein kinase 4 (HIPK4) |
CNY 5,890.00 |
|
| RG205549 | HIPK4 (tGFP-tagged) - Human homeodomain interacting protein kinase 4 (HIPK4) |
CNY 4,370.00 |
|
| SC111529 | HIPK4 (untagged)-Human homeodomain interacting protein kinase 4 (HIPK4) |
CNY 4,592.00 |
