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PRAK (MAPKAPK5) (NM_003668) Human Untagged Clone
CAT＃: SC323359
MAPKAPK5 (untagged)-Kinase deficient mutant (K45M) of Human mitogen-activated protein kinase-activated protein kinase 5 (MAPKAPK5), transcript variant 1
CNY 3,656.00
CNY 7,220.00
Product images
CNY 3,990.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | MAPKAP-K5; MK-5; MK5; PRAK |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323359 sequence for NM_003668 edited (data generated by NextGen Sequencing)
ATGTCGGAGGAGAGCGACATGGACAAAGCCATCAAGGAAACTTCCATTTTAGAAGAATAC AGTATCAATTGGACTCAGAAGCTGGGAGCTGGAATTAGTGGTCCAGTTAGAGTCTGTGTA AAGAAATCTACTCAAGAACGGTTTGCGCTGAWRATTCTTCTTGATCGTCCAAAAGCTAGA AATGAGGTACGTCTGCACATGATGTGTGCCACACACCCAAACATAGTTCAGATTATTGAA GTGTTTGCTAACAGTGTCCAGTTTCCCCATGAGTCCAGCCCTAGGGCCCGACTCTTAATT GTAATGGAGATGATGGAAGGGGGAGAGCTATTTCACAGAATCAGCCAGCACCGGCACTTT ACAGAGAAGCAAGCCAGCCAAGTAACAAAGCAGATAGCTTTGGCTCTGCGGCACTGTCAC TTGTTAAACATTGCGCACAGAGACCTCAAGCCTGAAAATCTGCTTTTTAAGGATAACTCT TTGGATGCCCCAGTGAAGTTGTGTGACTTTGGATTTGCCAAGATTGACCAAGGTGACTTG ATGACACCCCAGTTCACCCCTTATTATGTAGCACCCCAGGTACTGGAGGCGCAAAGAAGG CATCAGAAGGAGAAATCTGGCATCATACCTACCTCACCGACGCCCTACACTTACAACAAG AGCTGTGACTTGTGGTCCCTAGGGGTGATTATCTATGTGATGCTGTGCGGATACCCTCCT TTTTACTCCAAACACCACAGCCGGACTATCCCAAAGGATATGCGAAGAAAGATCATGACA GGCAGTTTTGAGTTCCCAGAGGAAGAGTGGAGTCAGATCTCAGAGATGGCCAAAGATGTT GTGAGGAAGCTCCTGAAGGTCAAACCGGAGGAGAGACTCACCATCGAGGGAGTGCTGGAC CACCCCTGGCTCAATTCCACCGAGGCCCTGGATAATGTGCTGCCTTCTGCTCAGCTGATG ATGGACAAGGCAGTGGTTGCAGGAATCCAGCAGGCTCACGCGGAACAGTTGGCCAACATG AGAATCCAGGATCTGAAAGTCAGCCTCAAACCCCTGCACTCAGTGAACAACCCCATTCTG CGGAAGAGGAAGTTACTTGGCACCAAGCCAAAGGACAGTGTCTATATCCACGACCATGAG AATGGAGCCGAGGATTCCAATGTTGCCTTGGAAAAACTCCGAGATGTGATTGCTCAGTGT ATTCTCCCCCAGGCTGGAGAGAATGAAGATGAGAAACTGAATGAAGTAATGCAGGAGGCT TGGAAGTATAACCGGGAATGCAAACTCCTAAGAGATACTCTGCAGAGCTTCAGCTGGAAT GGTCGTGGATTCACAGATAAAGTAGATCGACTAAAACTGGCAGAAATTGTGAAGCAGGTG ATAGAAGAGCAAACCACGTCCCACGAATCCCAATAA Clone variation with respect to NM_003668.2 152 a=>w;153 a=>r >OriGene 5' read for mutant NM_003668 unedited
TCGGCACAAAGGAACAACGAGCGGAGGCGCGCACGGGAGAGCCAACACAAGCAGAGCACGACAAGCGAAC CGACAGAATACGCAACACGACACACCACAGGGCGGCCGCGAACCCGGCACGAGGGCCGAGCCCCCCGCCC CCCCGGCCGCGCGGGGACAGGGCTGCTGAGCAGCCTCCGCCTCTCCCGGCTGTGGGGGCCCCACTGAGTA TGTCGGAGGAGAGCGACATGGACAAAGCCATCAAGGAAACTTCCATTTTAGAAGAATACAGTATCAATTG GACTCAGAAGCTGGGAGCTGGAATTAGTGGTCCAGTTAGAGTCTGTGTAAAGAAATCTACTCAAGAACGG TTTGCGCTGATGATTCTTCTTGATCGTCCAAAAGCTAGAACGGTTTGCGCTGATGATTCTTCTTGATCGT CCAAAAGCTAGAACGGTTTGCGCTGATGATTCTTCTTGATCGTCCAAAGCTAGAACGGTTTGCGCTGATG ATTCTCTGATCGTCCAAAGCTAGACGGTTTGCGCTGATGATCTCTGATCGTCCAAAGCTAGACGGTTGCG CTGATGATCTCTGATCGTCAAAGCTAAACGGTTTGCGCTGATGATTCTTCCTGATCGTCAAAGCTAAACG GTTGCGCCTGATGATCCTTCTGATTCGGTCAAGCTAAATGAGGTACGTCTGGCATGAAGTGTGGCCCACA CCAACTAGTTCAATATATGGAATGTTTGGCTACGTGCAGTTTCCATGAGTCAGCCTTAGGCGATCTTATT GTATTGGATGATTGAGAGGGGGAGCAATTCATGATCTGACGCGGGCTTACGTAAGCAGCACCGCGTATCA ACGATAGCTTGTCTGCGCAGTCTGCACTTGTTAC >SC323359 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
AWATTTTAGAGWAAGTATCAATTGGACTCAGAAGCTGGGAGCTGGAATTAGTGGTCCAGTTAGAGTCTGT GTAAAGAAATCTACTCAAGAACGGTTTGCGCTGATGATTCTTCTTGATCGTCCAAAAGCTAGAACGGTTT GCGCTGATGATTCTTCTTGATCGTCCAAAAGCTAGAACGGTTTGCGCTGATGATTCTTCTTGATCGTCCA AAAGCTAGAACGGTTTGCGCTGATGATTCTTCTTGATCGTCCAAA |
| Restriction Sites | Please inquire |
| ACCN | NM_003668 |
| Insert Size | 1900 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003668.2, NP_003659.2 |
| RefSeq Size | 2060 bp |
| RefSeq ORF | 1416 bp |
| Locus ID | 8550 |
| UniProt ID | Q8IW41 |
| Domains | pkinase, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | MAPK signaling pathway |
| Gene Summary | The protein encoded by this gene is a tumor suppressor and member of the serine/threonine kinase family. In response to cellular stress and proinflammatory cytokines, this kinase is activated through its phosphorylation by MAP kinases including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta. The encoded protein is found in the nucleus but translocates to the cytoplasm upon phosphorylation and activation. This kinase phosphorylates heat shock protein HSP27 at its physiologically relevant sites. Two alternately spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Nov 2012] Transcript Variant: This variant (1) is the more frequently occurring transcript. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216715 | MAPKAPK5 (Myc-DDK-tagged)-Human mitogen-activated protein kinase-activated protein kinase 5 (MAPKAPK5), transcript variant 1 |
CNY 3,656.00 |
|
| RC216715L1 | Lenti ORF clone of Human mitogen-activated protein kinase-activated protein kinase 5 (MAPKAPK5), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC216715L2 | Lenti ORF clone of Human mitogen-activated protein kinase-activated protein kinase 5 (MAPKAPK5), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC216715L3 | Lenti ORF clone of Human mitogen-activated protein kinase-activated protein kinase 5 (MAPKAPK5), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC216715L4 | Lenti ORF clone of Human mitogen-activated protein kinase-activated protein kinase 5 (MAPKAPK5), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG216715 | MAPKAPK5 (tGFP-tagged) - Human mitogen-activated protein kinase-activated protein kinase 5 (MAPKAPK5), transcript variant 1 |
CNY 5,256.00 |
|
| SC109943 | MAPKAPK5 (untagged)-Human mitogen-activated protein kinase-activated protein kinase 5 (MAPKAPK5), transcript variant 1 |
CNY 2,950.00 |
