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IKK alpha (CHUK) (NM_001278) Human Untagged Clone
CAT＃: SC323326
CHUK (untagged)-Kinase deficient mutant (K44M) of Human conserved helix-loop-helix ubiquitous kinase (CHUK)
CNY 8,192.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | BPS2; IKBKA; IKK-alpha; IKK1; IKKA; NFKBIKA; TCF16 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323326 sequence for NM_001278 edited (data generated by NextGen Sequencing)
ATGGAGCGGCCCCCGGGGCTGCGGCCGGGCGCGGGCGGGCCCTGGGAGATGCGGGAGCGG CTGGGCACCGGCGGCTTCGGGAACGTCTGTCTGTACCAGCATCGGGTACTTGATCTCAAA ATAGCAATTATGTCTTGTCGCCTAGAGCTAAGTACCAAAAACAGAGAACGATGGTGCCAT GAAATCCAGATTATGAAGAAGTTGAACCATGCCAATGTTGTAAAGGCCTGTGATGTTCCT GAAGAATTGAATATTTTGATTCATGATGTGCCTCTTCTAGCAATGGAATACTGTTCTGGA GGAGATCTCCGAAAGCTGCTCAACAAACCAGAAAATTGTTGTGGACTTAAAGAAAGCCAG ATACTTTCTTTACTAAGTGATATAGGGTCTGGGATTCGATATTTGCATGAAAACAAAATT ATACATCGAGATCTAAAACCTGAAAACATAGTTCTTCAGGATGTTGGTGGAAAGATAATA CATAAAATAATTGATCTGGGATATGCCAAAGATGTTGATCAAGGAAGTCTGTGTACATCT TTTGTGGGAACACTGCAGTATCTGGCCCCAGAGCTCTTTGAGAATAAGCCTTACACAGCC ACTGTTGATTATTGGAGCTTTGGGACCATGGTATTTGAATGTATTGCTGGATATAGGCCT TTTTTGCATCATCTGCAGCCATTTACCTGGCATGAGAAGATTAAGAAGAAGGATCCAAAG TGTATATTTGCATGTGAAGAGATGTCAGGAGAAGTTCGGTTTAGTAGCCATTTACCTCAA CCAAATAGCCTTTGTAGTTTAGTAGTAGAACCCATGGAAAACTGGCTACAGTTGATGTTG AATTGGGACCCTCAGCAGAGAGGAGGACCTGTTGACCTTACTTTGAAGCAGCCAAGATGT TTTGTATTAATGGATCACATTTTGAATTTGAAGATAGTACACATCCTAAATATGACTTCT GCAAAGATAATTTCTTTTCTGTTACCACCTGATGAAAGTCTTCATTCACTACAGTCTCGT ATTGAGCGTGAAACTGGAATAAATACTGGTTCTCAAGAACTTCTTTCAGAGACAGGAATT TCTCTGGATCCTCGGAAACCAGCCTCTCAATGTGTTCTAGATGGAGTTAGAGGCTGTGAT AGCTATATGGTTTATTTGTTTGATAAAAGTAAAACTGTATATGAAGGGCCATTTGCTTCC AGAAGTTTATCTGATTGTGTAAATTATATTGTACAGGACAGCAAAATACAGCTTCCAATT ATACAGCTGCGTAAAGTGTGGGCTGAAGCAGTGCACTATGTGTCTGGACTAAAAGAAGAC TATAGCAGGCTCTTTCAGGGACAAAGGGCAGCAATGTTAAGTCTTCTTAGATATAATGCT AACTTAACAAAAATGAAGAACACTTTGATCTCAGCATCACAACAACTGAAAGCTAAATTG GAGTTTTTTCACAAAAGCATTCAGCTTGACTTGGAGAGATACAGCGAGCAGATGACGTAT GGGATATCTTCAGAAAAAATGCTAAAAGCATGGAAAGAAATGGAAGAAAAGGCCATCCAC TATGCTGAGGTTGGTGTCATTGGATACCTGGAGGATCAGATTATGTCTTTGCATGCTGAA ATCATGGAGCTACAGAAGAGCCCCTATGGAAGACGTCAGGGAGACTTGATGGAATCTCTG GAACAGCGTGCCATTGATCTATATAAGCAGTTAAAACACAGACCTTCAGATCACTCCTAC AGTGACAGCACAGAGATGGTGAAAATCATTGTGCACACTGTGCAGAGTCAGGACCGTGTG CTCAAGGAGCTGTTTGGTCATTTGAGCAAGTTGTTGGGCTGTAAGCAGAAGATTATTGAT CTACTCCCTAAGGTGGAAGTGGCCCTCAGTAATATCAAAGAAGCTGACAATACTGTCATG TTCATGCAGGGAAAAAGGCAGAAAGAAATATGGCATCTCCTTAAAATTGCCTGTACACAG AGTTCTGCCCGGTCCCTTGTAGGATCCAGTCTAGAAGGTGCAGTAACCCCTCAGACATCA GCATGGCTGCCCCCGACTTCAGCAGAACATGATCATTCTCTGTCATGTGTGGTAACTCCT CAAGATGGGGAGACTTCAGCACAAATGATAGAAGAAAATTTGAACTGCCTTGGCCATTTA AGCACTATTATTCATGAGGCAAATGAGGAACAGGGCAATAGTATGATGAATCTTGATTGG AGTTGGTTAACAGAATGA Clone variation with respect to NM_001278.3 107 a=>t;131 a=>t >OriGene 5' read for mutant NM_001278 unedited
TCGGTACGACGAACAACGGGCGGAGGCGGCACGGGAGGGCCACACAAGCAGACACGAAAGCGAACCGACA GAGTACGAAAACGACACACAAAAGGGCGGCCGCGAACACGGCACGAGGCGGCCACGGAACAACCGCGGAA CCCGAGGCCGCCCGCCCACCCGCCCCACGGAGCGGCCCCCGGGGCAGCGGCCGGGCGCGGGCGGGCCCTG GGAGATGCGGGAGCGGCTGGGCACCGGCGGCTTTCGGGAACGTTCTGTCTGTACCAGCATCGGGTACTTG ATTCTCAAAATAGCAATTATGTCTTTGTCGCCTAGAGCTAAGTACCAAAAAACAGAGAAACGAATGGTTG CCATGAAATCCAGATTATGAAAGAAAGTTGACCCTGGCCATGGTTGGTAAAGGCCGGGGAGGTTCCTGAG AAATTGATTATTTTTGATTCTGGATGTGCCTCTTCTACCATGGGATTACGGTTCGGAAGGAAATCTCCAA AGGCGGCTCACCAAACCAAAAAATGTTTGGGGACTTAGAAAACCCAGTACCTTTTTTTCTTAATTGATTA GGGCCGGGTTCGATTTTTCATGAAAAAAAATTTTAATTCGATCTAAAACCTGAAACCAAGTCTTCCGAAT TTGTGGGAAGATATACATATAATATATGATCTGGAATGCCCAGATATGGTCAAGAGATCTGGGAAACTTT TGGAAACTGAGATCTGCCCAAGCTCTTGGATACCTACAGCACTGTGATATTGACTTGGACATGTATGATG ATGCGGATAAGCCTTTTGCTCTCGGACATACTGCTGGAGATAGAGAAGTCCAATGTATGCTGAAGAGTCG GAATCGTGTAAACAATACACAACCTGAATAAG >SC323326 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
CSCTGMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTC AGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGGCCTTGGAACAACTGTG GAACCTGAGGCCGCTTGCCCTCCCGCCCCATGGAGCGGCCCCCGGGGCTGCGGCCGGGCGCGGGCGGGCC CTGGGAGATGCGGGAGCGGCTGGGCACCGGCGGCTTCGGGAACGT |
| Restriction Sites | Please inquire |
| ACCN | NM_001278 |
| Insert Size | 3670 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001278.3, NP_001269.3 |
| RefSeq Size | 3539 bp |
| RefSeq ORF | 2238 bp |
| Locus ID | 1147 |
| UniProt ID | O15111 |
| Domains | pkinase, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Acute myeloid leukemia, Adipocytokine signaling pathway, Apoptosis, B cell receptor signaling pathway, Chemokine signaling pathway, Chronic myeloid leukemia, Cytosolic DNA-sensing pathway, Epithelial cell signaling in Helicobacter pylori infection, MAPK signaling pathway, NOD-like receptor signaling pathway, Pancreatic cancer, Pathways in cancer, Prostate cancer, RIG-I-like receptor signaling pathway, Small cell lung cancer, T cell receptor signaling pathway, Toll-like receptor signaling pathway |
| Gene Summary | This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216718 | CHUK (Myc-DDK-tagged)-Human conserved helix-loop-helix ubiquitous kinase (CHUK) |
CNY 8,184.00 |
|
| RC216718L1 | Lenti ORF clone of Human conserved helix-loop-helix ubiquitous kinase (CHUK), Myc-DDK-tagged |
CNY 10,584.00 |
|
| RC216718L2 | Lenti ORF clone of Human conserved helix-loop-helix ubiquitous kinase (CHUK), mGFP tagged |
CNY 5,890.00 |
|
| RC216718L3 | Lenti ORF clone of Human conserved helix-loop-helix ubiquitous kinase (CHUK), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC216718L4 | Lenti ORF clone of Human conserved helix-loop-helix ubiquitous kinase (CHUK), mGFP tagged |
CNY 10,584.00 |
|
| RG216718 | CHUK (tGFP-tagged) - Human conserved helix-loop-helix ubiquitous kinase (CHUK) |
CNY 9,784.00 |
|
| SC119358 | CHUK (untagged)-Human conserved helix-loop-helix ubiquitous kinase (CHUK) |
CNY 7,220.00 |
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