United States
Germany
Japan
United Kingdom
China
GEN1 (NM_001130009) Human Untagged Clone
CAT＃: SC323232
GEN1 (untagged)-Human Gen homolog 1, endonuclease (Drosophila) (GEN1), transcript variant 2
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | Gen |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC323232 representing NM_001130009.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGGAGTGAATGACTTGTGGCAAATTTTGGAGCCTGTTAAGCAACACATCCCCTTGCGTAATCTTGGT GGGAAAACCATTGCAGTTGATCTGAGTCTCTGGGTGTGTGAGGCACAGACAGTCAAAAAAATGATGGGC AGCGTCATGAAGCCCCACCTCAGGAACTTATTTTTTCGTATCTCATATTTAACACAAATGGATGTAAAA CTGGTATTTGTTATGGAAGGGGAACCACCAAAGCTGAAAGCTGATGTCATAAGCAAGAGGAATCAGTCT CGGTATGGGTCTTCTGGAAAATCGTGGTCTCAGAAAACAGGGAGATCACATTTTAAATCAGTCTTAAGA GAGTGCCTCCATATGCTCGAATGCTTAGGAATCCCCTGGGTTCAGGCTGCTGGGGAAGCTGAAGCCATG TGTGCTTATCTCAATGCTGGTGGTCATGTCGATGGCTGCCTCACCAATGATGGAGATACTTTCCTTTAT GGGGCCCAGACTGTTTACAGGAATTTCACTATGAATACAAAGGACCCACATGTTGACTGTTACACAATG TCATCTATCAAGAGTAAACTAGGTTTGGATAGAGATGCTCTGGTTGGATTAGCAATACTTCTTGGCTGT GATTATCTCCCAAAGGGAGTCCCTGGAGTTGGAAAAGAGCAAGCATTAAAACTTATACAGATTTTGAAA GGGCAAAGTTTACTTCAGAGGTTTAATCGGTGGAATGAAACATCTTGTAACTCTAGTCCACAACTGCTA GTCACTAAAAAACTGGCTCATTGTTCCGTATGTTCCCATCCAGGTTCACCTAAGGATCATGAACGTAAT GGATGCAGATTATGTAAAAGTGATAAATATTGTGAGCCACATGACTATGAATACTGCTGTCCTTGTGAG TGGCACCGTACAGAACATGATAGGCAACTCAATGAAGTAGAGAACAATATTAAGAAGAAAGCTTGCTGT TGTGAGGGATTCCCATTCCATGAGGTTATTCAAGAATTCCTTTTAAACAAGGATAAATTGGTGAAGGTT ATCAGGTACCAAAGACCTGATTTGTTATTGTTTCAGAGATTTACTCTTGAAAAAATGGAGTGGCCCAAT CACTATGCATGTGAGAAATTGCTGGTACTTTTGACCCATTATGACATGATAGAAAGAAAGCTTGGTAGC AGAAACTCTAATCAACTACAGCCAATTCGAATTGTTAAGACTCGAATCAGAAATGGAGTTCATTGTTTT GAAATAGAATGGGAAAAGCCTGAACATTATGCTATGGAAGATAAACAACATGGAGAATTTGCTTTATTA ACAATTGAGGAAGAATCATTGTTTGAAGCAGCATATCCTGAGATCGTTGCTGTTTACCAAAAACAAAAG TTAGAAATTAAAGGGAAGAAACAAAAACGTATTAAGCCTAAAGAAAACAATTTGCCAGAACCAGATGAA GTAATGAGCTTTCAGTCACACATGACTTTAAAACCCACATGTGAAATCTTTCATAAGCAGAATTCCAAG TTAAATTCGGGGATTTCCCCTGATCCTACATTACCACAGGAATCTATTTCTGCCTCATTGAATAGCTTG CTTTTACCTAAAAATACTCCATGTTTGAATGCACAAGAACAGTTCATGTCTTCTCTAAGACCTTTGGCT ATACAGCAAATTAAAGCTGTCAGTAAGTCTCTAATTTCAGAATCTAGTCAACCCAATACCTCATCTCAT AATATATCCGTGATTGCTGATCTACACTTGAGCACTATTGACTGGGAAGGTACTTCTTTTAGTAATTCT CCAGCTATTCAAAGGAATACTTTTTCTCATGATTTAAAATCAGAAGTTGAATCAGAGCTATCAGCCATC CCTGATGGCTTTGAAAATATCCCAGAACAACTGTCCTGTGAATCAGAAAGGTACACTGCAAACATAAAG AAAGTGTTGGATGAGGATTCTGATGGGATTAGTCCTGAAGAGCATCTACTTTCTGGCATTACTGATTTA TGTCTTCAGGATTTGCCTTTAAAGGAACGAATATTTACAAAATTATCATATCCTCAGGATAATCTACAA CCAGATGTCAACCTGAAAACTTTGTCCATACTTAGTGTAAAAGAATCTTGTATTGCTAACAGTGGTTCT GATTGTACATCACATCTTTCAAAGGATCTTCCAGGAATTCCCTTGCAAAATGAATCCAGAGACTCTAAA ATTCTAAAAGGAGACCAGCTGCTTCAAGAAGACTATAAAGTCAATACTTCTGTCCCTTATTCTGTCAGT AACACAGTGGTAAAGACCTGCAATGTTAGACCACCAAATACTGCTTTAGATCATAGTAGAAAAGTTGAT ATGCAAACCACTCGGAAAATTTTAATGAAGAAGAGTGTTTGCCTTGACAGACATTCCTCTGATGAACAA AGTGCCCCAGTGTTTGGGAAAGCTAAGTACACAACTCAAAGAATGAAGCACAGTTCTCAAAAGCATAAT TCATCCCATTTCAAAGAAAGTGGCCATAACAAGTTGAGTAGCCCTAAGATACATATTAAAGAAACTGAA CAGTGTGTCAGATCTTATGAAACAGCTGAAAATGAAGAAAGCTGTTTCCCAGATTCAACAAAAAGTTCT CTGAGTTCTCTACAATGTCATAAGAAAGAAAACAACTCTGGTACTTGTTTGGATAGCCCTCTTCCTTTA CGCCAGAGATTAAAACTAAGATTCCAAAGCACTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001130009 |
| Insert Size | 2727 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001130009.2 |
| RefSeq Size | 6931 bp |
| RefSeq ORF | 2727 bp |
| Locus ID | 348654 |
| UniProt ID | Q17RS7 |
| MW | 102.9 kDa |
| Gene Summary | This gene encodes a member of the Rad2/xeroderma pigmentosum group G nuclease family, whose members are characterized by N-terminal and internal xeroderma pigmentosum group G nuclease domains followed by helix-hairpin-helix domains and disordered C-terminal domains. The protein encoded by this gene is involved in resolution of Holliday junctions, which are intermediate four-way structures that covalently link DNA during homologous recombination and double-strand break repair. The protein resolves Holliday junctions by creating dual incisions across the junction to produce nicked duplex products that can be ligated. In addition, this protein has been found to localize to centrosomes where it has been implicated in regulation of centrosome integrity. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2016] Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because transcript sequence data consistent with the reference genome assembly was not available for all regions of the RefSeq transcript. The extent of this transcript is supported by transcript alignments. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC226255 | GEN1 (Myc-DDK-tagged)-Human Gen homolog 1, endonuclease (Drosophila) (GEN1), transcript variant 2 |
CNY 3,990.00 |
|
| RC226255L1 | Lenti ORF clone of Human Gen homolog 1, endonuclease (Drosophila) (GEN1), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC226255L2 | Lenti ORF clone of Human Gen homolog 1, endonuclease (Drosophila) (GEN1), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RC226255L3 | Lenti ORF clone of Human Gen homolog 1, endonuclease (Drosophila) (GEN1), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC226255L4 | Lenti ORF clone of Human Gen homolog 1, endonuclease (Drosophila) (GEN1), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG226255 | GEN1 (tGFP-tagged) - Human Gen homolog 1, endonuclease (Drosophila) (GEN1), transcript variant 2 |
CNY 4,370.00 |
